##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10443046
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.12633679866966	28.0	18.0	32.0	18.0	33.0
2	28.09128945711816	29.0	27.0	32.0	18.0	33.0
3	30.059536460913797	31.0	29.0	33.0	25.0	33.0
4	30.12624056237998	31.0	29.0	33.0	25.0	33.0
5	31.63824855315202	33.0	32.0	33.0	30.0	33.0
6	35.50429836275738	37.0	36.0	38.0	29.0	38.0
7	35.905210031632535	38.0	36.0	38.0	31.0	38.0
8	36.3575063252618	38.0	37.0	38.0	33.0	38.0
9	36.557104986418715	38.0	38.0	38.0	34.0	38.0
10-11	36.701783224932655	38.0	38.0	38.0	34.0	38.0
12-13	36.76328520433598	38.0	38.0	38.0	34.0	38.0
14-15	36.78538474311039	38.0	38.0	38.0	34.0	38.0
16-17	36.80433591884973	38.0	38.0	38.0	35.0	38.0
18-19	36.81587938997875	38.0	38.0	38.0	35.0	38.0
20-21	36.82658275922539	38.0	38.0	38.0	35.0	38.0
22-23	36.84365351177627	38.0	38.0	38.0	35.0	38.0
24-25	36.8512236074645	38.0	38.0	38.0	35.0	38.0
26-27	36.807628537065675	38.0	38.0	38.0	35.0	38.0
28-29	36.79431652054576	38.0	38.0	38.0	35.0	38.0
30-31	36.770294379669544	38.0	38.0	38.0	35.0	38.0
32-33	36.75511903128301	38.0	38.0	38.0	35.0	38.0
34-35	36.7363155455853	38.0	38.0	38.0	34.5	38.0
36-37	36.71429840223993	38.0	38.0	38.0	34.0	38.0
38-39	36.70225042684713	38.0	38.0	38.0	34.0	38.0
40-41	36.68643003244648	38.0	38.0	38.0	34.0	38.0
42-43	36.640662655050775	38.0	38.0	38.0	34.0	38.0
44-45	36.59633435641588	38.0	38.0	38.0	34.0	38.0
46-47	36.55557535997484	38.0	38.0	38.0	34.0	38.0
48-49	36.51608642509068	38.0	38.0	38.0	34.0	38.0
50-51	36.49280916760861	38.0	38.0	38.0	34.0	38.0
52-53	36.46455562169949	38.0	38.0	38.0	34.0	38.0
54-55	36.39681296928301	38.0	38.0	38.0	34.0	38.0
56-57	36.40625594807699	38.0	38.0	38.0	34.0	38.0
58-59	36.403385776634295	38.0	38.0	38.0	34.0	38.0
60-61	36.36173119034642	38.0	38.0	38.0	33.5	38.0
62-63	36.37020245012886	38.0	38.0	38.0	33.5	38.0
64-65	36.394632539865	38.0	38.0	38.0	34.0	38.0
66-67	36.37748463893124	38.0	38.0	38.0	34.0	38.0
68-69	36.36300682395839	38.0	38.0	38.0	34.0	38.0
70-71	36.364644833209354	38.0	38.0	38.0	34.0	38.0
72-73	36.35497303338735	38.0	38.0	38.0	34.0	38.0
74-75	36.345907536766006	38.0	38.0	38.0	33.5	38.0
76-77	36.31114704033236	38.0	38.0	38.0	33.0	38.0
78-79	36.30753861856013	38.0	38.0	38.0	33.0	38.0
80-81	36.292672286790385	38.0	37.5	38.0	33.0	38.0
82-83	36.260179503158355	38.0	37.0	38.0	33.0	38.0
84-85	36.22186412214906	38.0	37.0	38.0	33.0	38.0
86-87	36.238837645341086	38.0	37.0	38.0	33.0	38.0
88-89	36.23679875904492	38.0	37.0	38.0	33.0	38.0
90-91	36.220544868736056	38.0	37.0	38.0	33.0	38.0
92-93	36.21163569552657	38.0	37.0	38.0	33.0	38.0
94-95	36.195276839653005	38.0	37.0	38.0	33.0	38.0
96-97	36.18747681336727	38.0	37.0	38.0	33.0	38.0
98-99	36.237063766520535	38.0	37.0	38.0	33.0	38.0
100-101	35.71792794898893	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	29.0
19	48.0
20	95.0
21	495.0
22	2119.0
23	6364.0
24	15146.0
25	29021.0
26	48499.0
27	73467.0
28	104785.0
29	141964.0
30	182778.0
31	231936.0
32	294785.0
33	386482.0
34	536436.0
35	849934.0
36	1946844.0
37	5591817.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.440571266276145	23.1041977599256	14.021196497650207	23.434034476148053
2	15.821188568929026	20.91735495563268	40.702291266360405	22.55916520907789
3	18.589943704386936	26.60042410357749	29.439183156285154	25.370449035750415
4	13.68537493754217	18.430906078552177	37.47925653109256	30.404462452813096
5	14.157318957961849	37.114398632975444	35.21239810643033	13.515884302632381
6	30.688191931740988	37.63279411007095	18.05119885519991	13.62781510298815
7	27.013651306827818	32.69160441613681	22.376904921753887	17.917839355281487
8	24.424990563098163	37.28861292002353	20.263034367559044	18.02336214931927
9	25.28273838878044	17.386651365894586	21.114318561844886	36.21629168348009
10-11	24.515668129777463	26.912095379068525	28.190845850913615	20.381390640240404
12-13	25.25742968095707	24.306557684415065	28.5451821240661	21.890830510561766
14-15	22.37370529255142	25.767787353582566	26.27815576195237	25.58035159191364
16-17	21.829904799806492	28.834805477252516	27.103701353034353	22.231588369906635
18-19	21.807315605044735	27.904119162167817	28.90984105595245	21.378724176834996
20-21	22.67043708574012	26.95851073904868	28.957764584088036	21.41328759112317
22-23	22.381835147816435	26.85382956742381	28.813378424397136	21.950956860362624
24-25	22.17038031738145	26.98465883357103	28.907076574047196	21.93788427500033
26-27	22.040973085271855	27.119248257953487	28.84917049931347	21.990608157461185
28-29	21.952954785134672	27.160151655890452	28.944047761180304	21.942845797794575
30-31	22.273099450678423	27.001297838462392	28.810210845402818	21.915391865456375
32-33	22.048563525886074	27.140567023679868	28.779159752137275	22.03170969829679
34-35	22.217501964446054	27.17004940568583	28.767630283208725	21.84481834665939
36-37	22.205232762262504	27.02320713925396	28.758325369876502	22.013234728607035
38-39	22.047434617521255	27.21482959917878	28.810627101055854	21.927108682244114
40-41	22.301172954846894	27.072306422759006	28.686005302791006	21.94051531960309
42-43	22.184055831808227	27.14079345865148	28.520197918380184	22.154952791160113
44-45	22.230441936240126	27.23585811412	28.40529584258753	22.128404107052344
46-47	22.29961236062858	27.24198188232692	28.112040334392525	22.346365422651974
48-49	22.26009178048933	27.32025884470496	28.138999578366825	22.280649796438887
50-51	22.245777616886926	27.456881175460857	28.01956802023451	22.277773187417708
52-53	22.388600289734875	27.386286924118235	27.873901100275695	22.351211685871192
54-55	22.401525384083023	27.30316880212664	27.905617219487734	22.38968859430261
56-57	22.317904963292854	27.40429458774529	27.92808686618481	22.349713582777046
58-59	22.326135003081635	27.38796016775889	27.95799371234906	22.327911116810416
60-61	22.315118765556168	27.288830435512057	28.04828212201099	22.347768676920783
62-63	22.454443321549345	27.34962901696754	28.02519258856919	22.17073507291392
64-65	22.215263599584155	27.282064961596543	28.025295395300127	22.47737604351918
66-67	22.288179829159215	27.39618752073398	27.804476758440888	22.511155891665915
68-69	22.220369925879194	27.523864815240724	27.910958784014568	22.344806474865518
70-71	22.220167796072026	27.29404415631692	27.9622574467431	22.523530600867954
72-73	22.258263667726236	27.364167224673768	27.693577525253538	22.683991582346458
74-75	22.179264806934217	27.46958252639502	27.66676065848711	22.68439200818365
76-77	22.354629009350607	27.243206996167068	27.77003870429497	22.632125290187357
78-79	22.337347538831075	27.24710111225849	27.637568368931536	22.777982979978898
80-81	22.505851791227027	27.307989601286987	27.46770352925208	22.718455078233905
82-83	22.574600339239286	27.434453845061906	27.568757578862918	22.42218823683589
84-85	22.50157768156654	27.276655291920303	27.43071227835745	22.7910547481557
86-87	22.378880295178856	27.49547270531123	27.508462510213477	22.61718448929644
88-89	22.60807678767651	27.294035022127368	27.502450892748083	22.595437297448036
90-91	22.624125247265546	27.543095363385707	27.480474830934103	22.352304558414648
92-93	22.541366709892497	27.463712656564088	27.45318637756094	22.541734255982476
94-95	22.548569261998605	27.343516637394067	27.514852842387562	22.59306125821977
96-97	22.59622184818903	27.57335708577033	27.51281476120403	22.317606304836616
98-99	22.873690317423417	27.95466861819057	27.662494443152948	21.509146621233068
100-101	23.34573028764244	30.578641904193837	24.989634381614735	21.085993426548992
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2703.0
1	2501.5
2	2736.5
3	3349.0
4	4109.0
5	5805.0
6	7597.5
7	10182.0
8	12863.0
9	14189.0
10	13969.5
11	13325.0
12	14508.5
13	17846.5
14	25222.5
15	34630.0
16	41605.0
17	45011.0
18	45041.0
19	42408.5
20	38354.0
21	34580.5
22	33165.5
23	36062.0
24	43821.5
25	56307.5
26	72876.5
27	90681.5
28	110524.5
29	133275.5
30	154775.5
31	176769.0
32	201550.0
33	228286.5
34	255390.0
35	280430.5
36	306785.0
37	329458.0
38	345941.0
39	360819.5
40	377018.5
41	391562.5
42	399824.0
43	409009.0
44	418536.5
45	426486.0
46	433202.5
47	434315.0
48	433312.0
49	433305.5
50	429294.5
51	419749.5
52	405913.5
53	385593.0
54	361063.5
55	335749.5
56	308568.0
57	279607.0
58	250422.0
59	217341.0
60	180001.5
61	144454.5
62	113759.5
63	87569.0
64	65480.5
65	48405.0
66	35856.5
67	27612.0
68	21308.0
69	15978.0
70	12032.0
71	8766.5
72	6144.5
73	4305.0
74	2896.0
75	1785.5
76	1105.5
77	674.5
78	420.0
79	268.0
80	179.5
81	125.0
82	88.5
83	57.5
84	37.0
85	27.0
86	20.5
87	14.5
88	7.5
89	2.0
90	0.5
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003006785568118727
4	0.0
5	0.0497364466267792
6	0.0
7	0.0015608472853609952
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.4363625325407931E-5
16-17	0.0
18-19	0.0
20-21	5.266748103360219E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	5.493866647274982E-4
48-49	1.9133066651950986E-4
50-51	5.782298588464832E-4
52-53	0.0011998110731534
54-55	0.0029745214513856337
56-57	0.0027101243729863492
58-59	0.00639568376760356
60-61	0.005789101313404321
62-63	0.0069806523712038025
64-65	0.003927339548012044
66-67	0.006488937191260594
68-69	0.006964508841365752
70-71	0.0034878078534550465
72-73	0.0027545211871240075
74-75	0.0037617441791572706
76-77	0.002187202921210367
78-79	0.0
80-81	6.138652028179369E-4
82-83	2.581985054208203E-4
84-85	0.0
86-87	5.843366686101704E-6
88-89	1.18015767850711E-5
90-91	1.1924312098359358E-5
92-93	7.591877992013344E-4
94-95	6.698763079487156E-5
96-97	6.165833672813859E-6
98-99	1.9117589424914232E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	103.0
20-21	425.0
22-23	1060.0
24-25	1174.0
26-27	2734.0
28-29	6250.0
30-31	18569.0
32-33	24465.0
34-35	33882.0
36-37	52431.0
38-39	58234.0
40-41	60155.0
42-43	69433.0
44-45	81664.0
46-47	82715.0
48-49	75035.0
50-51	66861.0
52-53	59393.0
54-55	64293.0
56-57	66040.0
58-59	38703.0
60-61	71661.0
62-63	68866.0
64-65	72911.0
66-67	77959.0
68-69	79263.0
70-71	78004.0
72-73	73886.0
74-75	75847.0
76-77	80180.0
78-79	83188.0
80-81	83559.0
82-83	78471.0
84-85	78718.0
86-87	82206.0
88-89	86615.0
90-91	88326.0
92-93	86802.0
94-95	94441.0
96-97	155180.0
98-99	924187.0
100-101	7059157.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.35244026533597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.87995437674576	73.19481133596742
2	7.45361704758062	12.276471053431456
3	1.9084039704910953	4.714851719459937
4	0.7155224509683356	2.3570007960750656
5	0.3603152198621476	1.483641881019445
6	0.2006012017114962	0.9911999088660362
7	0.12749858603401748	0.7349873783196855
8	0.08041253870085438	0.5297735031956902
9	0.05130792281953883	0.38027993842210855
>10	0.21585982430899273	2.802699457116201
>50	0.005391180527518157	0.3041601654999731
>100	0.0010246023786992446	0.14707375701596992
>500	4.553895712750636E-5	0.027170519492151185
>1k	4.553891380807821E-5	0.055878586118877235
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9151500433877243E-5	0.0	0.0	0.0
3	0.0	1.9151500433877243E-5	0.0	0.0	0.0
4	0.0	3.8303000867754486E-5	0.0	0.0	0.0
5	1.9151500433877243E-5	3.8303000867754486E-5	0.0	0.0	0.0
6	1.9151500433877243E-5	3.8303000867754486E-5	0.0	0.0	0.0
7	1.9151500433877243E-5	3.8303000867754486E-5	0.0	0.0	0.0
8	1.9151500433877243E-5	3.8303000867754486E-5	0.0	0.0	0.0
9	1.9151500433877243E-5	1.2448475282020207E-4	0.0	0.0	0.0
10-11	3.8303000867754486E-5	1.340605030371407E-4	0.0	4.787875108469311E-5	0.0
12-13	3.8303000867754486E-5	1.3884837814561E-4	0.0	5.2666626193162414E-5	0.0
14-15	6.224237641010104E-5	1.8672712923030314E-4	0.0	6.224237641010104E-5	0.0
16-17	6.703025151857035E-5	1.9151500433877244E-4	0.0	6.703025151857035E-5	0.0
18-19	7.660600173550897E-5	2.1066650477264966E-4	0.0	6.703025151857035E-5	4.787875108469311E-6
20-21	8.139387684397828E-5	2.298180052065269E-4	0.0	6.703025151857035E-5	9.575750216938621E-6
22-23	1.0533325238632483E-4	2.4896950564040415E-4	0.0	6.703025151857035E-5	9.575750216938621E-6
24-25	1.340605030371407E-4	2.824846313996893E-4	0.0	9.09696270609169E-5	9.575750216938621E-6
26-27	1.675756287964259E-4	2.9684825672509724E-4	0.0	1.5799987857948724E-4	9.575750216938621E-6
28-29	1.723635039048952E-4	2.9684825672509724E-4	0.0	3.6866638335213695E-4	9.575750216938621E-6
30-31	1.771513790133645E-4	3.159997571589745E-4	0.0	0.0013597565308052842	9.575750216938621E-6
32-33	1.819392541218338E-4	3.159997571589745E-4	0.0	0.0036962395837383077	9.575750216938621E-6
34-35	1.819392541218338E-4	3.830300086775448E-4	0.0	0.007100418785859987	9.575750216938621E-6
36-37	1.9151500433877244E-4	4.4048450997917656E-4	0.0	0.012046293772908785	9.575750216938621E-6
38-39	1.9151500433877244E-4	4.596360104130538E-4	0.0	0.021368286609098532	9.575750216938621E-6
40-41	2.0109075455571103E-4	4.979390112808083E-4	0.0	0.05332256508302271	9.575750216938621E-6
42-43	2.0587862966418033E-4	5.123026366062162E-4	0.0	0.06257273979258543	9.575750216938621E-6
44-45	2.2024225498958828E-4	5.889086383417251E-4	0.0	0.07547127533480175	9.575750216938621E-6
46-47	2.4896950564040415E-4	6.511510147518263E-4	0.0	0.09027538517018885	9.575750216938621E-6
48-49	2.681210060742814E-4	6.559388898602955E-4	0.0	0.10408361698301434	9.575750216938621E-6
50-51	2.7769675629122E-4	7.229691413788659E-4	0.0	0.11738433403434209	9.575750216938621E-6
52-53	3.0163613183356656E-4	7.277570164873353E-4	0.0	0.1408640735662756	9.575750216938621E-6
54-55	3.447270078097904E-4	7.421206418127431E-4	0.0	0.20062154279508104	9.575750216938621E-6
56-57	3.7824213356907555E-4	7.612721422466204E-4	0.0	0.2652147658834405	9.575750216938621E-6
58-59	3.8303000867754487E-4	7.708478924635591E-4	0.0	0.3284051415650185	9.575750216938621E-6
60-61	3.8781788378601414E-4	7.947872680059056E-4	0.0	0.6595728870676238	9.575750216938621E-6
62-63	4.021815091114221E-4	8.043630182228441E-4	0.0	0.6654380340754986	9.575750216938621E-6
64-65	4.117572593283607E-4	8.522417693075373E-4	0.0	0.6714563930868446	9.575750216938621E-6
66-67	4.117572593283607E-4	8.570296444160066E-4	0.0	0.6766703890799677	9.575750216938621E-6
68-69	4.117572593283607E-4	8.713932697414146E-4	0.0	0.6816641428181012	9.575750216938621E-6
70-71	4.117572593283607E-4	8.953326452837611E-4	0.0	0.6863658361746181	9.575750216938621E-6
72-73	4.117572593283607E-4	9.096962706091691E-4	0.0	0.6900285606325971	9.575750216938621E-6
74-75	4.213330095452993E-4	9.288477710430463E-4	0.0	0.6931358915779937	9.575750216938621E-6
76-77	4.3090875976223796E-4	9.623628968023314E-4	0.0	0.6960708590194853	9.575750216938621E-6
78-79	4.356966348707073E-4	9.767265221277393E-4	0.0	0.6990106143360855	9.575750216938621E-6
80-81	4.452723850876459E-4	9.767265221277393E-4	0.0	0.7001022498608165	9.575750216938621E-6
82-83	4.500602601961152E-4	9.767265221277393E-4	0.0	0.7005906131218804	9.575750216938621E-6
84-85	4.500602601961152E-4	9.958780225616166E-4	0.0	0.7009927946309917	9.575750216938621E-6
86-87	4.500602601961152E-4	0.0010102416478870247	0.0	0.7012178247610898	9.575750216938621E-6
88-89	4.548481353045845E-4	0.001062908274080187	0.0	0.7012896428877169	9.575750216938621E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15160	0.0	51.802704	1
GTATCAA	26270	0.0	35.233414	1
TCAACGC	31770	0.0	29.393898	4
ATCAACG	31975	0.0	29.178623	3
TATCAAC	32650	0.0	28.695454	2
CAACGCA	32845	0.0	28.445112	5
AACGCAG	34180	0.0	27.38536	6
GTGGTAT	7135	0.0	25.517504	1
TGGTATC	6920	0.0	25.366165	2
ACGCAGA	39290	0.0	23.812578	7
CGCAGAG	40290	0.0	23.242828	8
CGAGACC	8260	0.0	22.637308	88-89
AGACCGA	8450	0.0	22.239134	90-91
ACCGAGG	8780	0.0	22.025812	92-93
GAGACCG	8675	0.0	21.90459	88-89
GACCGAG	8720	0.0	21.903297	90-91
ACGAGAC	8935	0.0	21.220037	86-87
ATCTCCG	8555	0.0	21.04811	74-75
TCTCCGA	8665	0.0	20.494339	76-77
GAGTACT	27180	0.0	20.462433	12-13
>>END_MODULE