FastQCFastQC Report
Fri 27 May 2016
SRR2139502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13960847
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197100.050.9181861
GTATCAA348250.035.15381
TATCTCG144100.034.8715194-95
CCGTCTT76600.030.016292-93
TCAACGC416050.029.6241344
ATCAACG419750.029.4165153
TATCAAC430200.028.8854872
CAACGCA429050.028.7469835
TATGCCG76800.028.5611988-89
AACGCAG442550.027.9251236
TGCCGTC81400.027.36183590-91
TGGTATC95300.026.8796442
TCTCGTA72000.026.728282-83
GTGGTAT102900.026.3436931
GCCGTCT80250.025.98683490-91
ATGCCGT82150.025.13049388-89
CTCGTAT88200.024.2190884-85
ACGCAGA511500.024.1351177
CGCAGAG527500.023.4111168
GCTATCT229250.023.3480694-95
Produced by FastQC (version 0.11.5)