FastQCFastQC Report
Fri 27 May 2016
SRR2139501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12006982
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167750.050.9764181
GTATCAA300950.037.15061
TCAACGC382050.029.207534
ATCAACG389200.028.660363
CAACGCA391500.028.502525
TATCAAC398550.028.0528742
AACGCAG404250.027.6853226
TGGTATC77950.025.9326522
GTGGTAT82300.025.7141321
ACGCAGA462050.024.1940657
CGCAGAG477000.023.4626798
GCAGAGT536250.020.8542339
GAGTACT313750.019.4349312-13
GTACATG383950.019.3644261
AGAGTAC490650.019.3024110-11
TACATGG395600.018.8377462
CAGAGTA527750.018.56991810-11
ACATGGG391800.018.3163953
GTACTTT342400.017.76484314-15
AGTACTT344650.017.66122212-13
Produced by FastQC (version 0.11.5)