##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12006982
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.92846528794663	31.0	25.0	33.0	18.0	33.0
2	30.872120654465878	33.0	30.0	33.0	27.0	33.0
3	31.280430502852425	33.0	31.0	33.0	28.0	34.0
4	31.79852289276356	33.0	32.0	33.0	29.0	34.0
5	32.15627282526117	33.0	33.0	34.0	31.0	34.0
6	35.4980662084777	38.0	36.0	38.0	29.0	38.0
7	35.78505073131616	38.0	36.0	38.0	31.0	38.0
8	36.05738552785371	38.0	37.0	38.0	32.0	38.0
9	36.54554633295861	38.0	37.0	38.0	34.0	38.0
10-11	36.646668163573494	38.0	38.0	38.0	34.0	38.0
12-13	36.71645351846117	38.0	38.0	38.0	34.0	38.0
14-15	36.70535497596315	38.0	38.0	38.0	34.0	38.0
16-17	36.72515508060227	38.0	38.0	38.0	34.0	38.0
18-19	36.758575635409464	38.0	38.0	38.0	34.5	38.0
20-21	36.782197137258336	38.0	38.0	38.0	35.0	38.0
22-23	36.80773948327662	38.0	38.0	38.0	35.0	38.0
24-25	36.80498772721029	38.0	38.0	38.0	35.0	38.0
26-27	36.772490016657045	38.0	38.0	38.0	35.0	38.0
28-29	36.76701584452235	38.0	38.0	38.0	35.0	38.0
30-31	36.75075500508676	38.0	38.0	38.0	35.0	38.0
32-33	36.73476637984386	38.0	38.0	38.0	34.5	38.0
34-35	36.70797603491556	38.0	38.0	38.0	34.0	38.0
36-37	36.68373162498142	38.0	38.0	38.0	34.0	38.0
38-39	36.67540399547579	38.0	38.0	38.0	34.0	38.0
40-41	36.66146962439171	38.0	38.0	38.0	34.0	38.0
42-43	36.62508631608986	38.0	38.0	38.0	34.0	38.0
44-45	36.579449186434914	38.0	38.0	38.0	34.0	38.0
46-47	36.538378204793325	38.0	38.0	38.0	34.0	38.0
48-49	36.495576411824985	38.0	38.0	38.0	34.0	38.0
50-51	36.47633603486855	38.0	38.0	38.0	34.0	38.0
52-53	36.44424974182366	38.0	38.0	38.0	34.0	38.0
54-55	36.37184437141744	38.0	38.0	38.0	34.0	38.0
56-57	36.37861753544399	38.0	38.0	38.0	34.0	38.0
58-59	36.37526667804441	38.0	38.0	38.0	34.0	38.0
60-61	36.33193876991483	38.0	38.0	38.0	33.5	38.0
62-63	36.33452313507773	38.0	38.0	38.0	33.0	38.0
64-65	36.353172465244356	38.0	38.0	38.0	34.0	38.0
66-67	36.33318911074278	38.0	38.0	38.0	33.0	38.0
68-69	36.31897727066328	38.0	38.0	38.0	33.0	38.0
70-71	36.320319052716115	38.0	38.0	38.0	33.0	38.0
72-73	36.3074080696615	38.0	38.0	38.0	33.0	38.0
74-75	36.29325548803739	38.0	37.0	38.0	33.0	38.0
76-77	36.25528730198138	38.0	37.0	38.0	33.0	38.0
78-79	36.25076559707796	38.0	37.0	38.0	33.0	38.0
80-81	36.24137888996356	38.0	37.0	38.0	33.0	38.0
82-83	36.21275315053196	38.0	37.0	38.0	33.0	38.0
84-85	36.166001926536225	38.0	37.0	38.0	33.0	38.0
86-87	36.177733365495	38.0	37.0	38.0	33.0	38.0
88-89	36.17380154451017	38.0	37.0	38.0	33.0	38.0
90-91	36.166073996832964	38.0	37.0	38.0	33.0	38.0
92-93	36.15210296274425	38.0	37.0	38.0	33.0	38.0
94-95	36.13488609748646	38.0	37.0	38.0	33.0	38.0
96-97	36.125860516932946	38.0	37.0	38.0	33.0	38.0
98-99	36.16951309551588	38.0	37.0	38.0	33.0	38.0
100-101	35.63981939462646	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	16.0
19	32.0
20	110.0
21	612.0
22	2668.0
23	8207.0
24	18434.0
25	34472.0
26	56878.0
27	84353.0
28	120325.0
29	160964.0
30	207522.0
31	264398.0
32	337015.0
33	437739.0
34	603408.0
35	934593.0
36	1987543.0
37	6747685.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.39318123405199	20.02137589612444	15.969175268189792	23.616267601633783
2	14.507683945890815	21.579194505330314	41.09803779167821	22.81508375710066
3	18.12811740079225	25.944220498114497	30.99953300672955	24.928129094363708
4	12.836073211403164	18.231925391409764	37.76922460614999	31.162776791037082
5	13.771126083108722	37.171490662740176	35.174276879706966	13.883106374444129
6	30.28447948035568	37.79894064969865	18.298445021405048	13.618134848540626
7	26.760456280097877	32.64471551622183	22.709104780180333	17.885723423499957
8	24.47688353326423	37.084239819798185	20.528447531611192	17.910429115326398
9	25.363909098889298	17.28440169228204	21.18048482124817	36.17120438758049
10-11	24.403349184944993	26.877997058544523	28.4026351003637	20.316018656146788
12-13	25.206517341326908	24.338930465624085	28.72990481704728	21.72464737600173
14-15	22.273177768264794	25.727664241965925	26.467675468769	25.53148252100029
16-17	21.747933827168225	28.82332129755837	27.172706680163262	22.256038195110143
18-19	21.812804416630257	27.849579519649488	29.106235855104973	21.23138020861529
20-21	22.601039482650663	26.94285843736794	29.089385780089223	21.36671629989218
22-23	22.39670401369849	26.7286018282614	28.989555344281502	21.88513881375861
24-25	22.128125558500813	26.967671125065905	29.000521416672314	21.903681899760965
26-27	21.98610707969409	27.066354865488044	29.021607858336136	21.92593019648173
28-29	21.982060942554586	27.007942460863095	29.080105833912967	21.929890762669356
30-31	22.180703354755916	26.94610513656861	28.960177638122648	21.913013870552824
32-33	21.97835113089485	27.13919387581059	28.90930852042233	21.973146472872234
34-35	22.16351814212074	27.096336320146957	28.906081693045216	21.834063844687087
36-37	22.1217838524583	26.985275909536266	28.881792370301046	22.01114786770438
38-39	22.034241997416682	27.15475207272079	28.928019992458793	21.88298593740374
40-41	22.220941116146484	26.97470819648969	28.849987577389918	21.954363109973908
42-43	22.090218632363616	27.10383044806993	28.65465434189597	22.15129657767048
44-45	22.1075772476507	27.17861121509405	28.556011178101205	22.15780035915405
46-47	22.273299297009405	27.165173778343448	28.299509471033364	22.26201745361378
48-49	22.185991436917345	27.2899547180471	28.250716799568877	22.27333704546668
50-51	22.18912690624782	27.422162883181567	28.112931087236152	22.27577912333446
52-53	22.375121877535648	27.348784796424784	27.96098542002471	22.31510790601486
54-55	22.337354595715787	27.28126006977479	27.975077962959343	22.406307371550078
56-57	22.255399150468648	27.357463107978475	28.058977342050895	22.328160399501982
58-59	22.2447769271326	27.29941016534065	28.075329900839492	22.38048300668726
60-61	22.324292252392954	27.33241563863845	28.055837611176926	22.28745449779167
62-63	22.29826293864127	27.424286244259022	28.054875500575054	22.22257531652465
64-65	22.25812194632659	27.32227657636445	28.003666152831343	22.41593532447762
66-67	22.297521371923686	27.41341009946551	27.872986200336356	22.41608232827445
68-69	22.225140630083253	27.391374376619577	27.95357515270409	22.429909840593087
70-71	22.218936388446153	27.20912069563466	28.002522762811427	22.569420153107764
72-73	22.295242511519113	27.27414231964971	27.84641194685195	22.58420322197923
74-75	22.189403823148428	27.39858638200443	27.866364625370636	22.5456451694765
76-77	22.411375875737686	27.310027450810736	27.765759021500212	22.512837651951358
78-79	22.348542377578944	27.280054335702935	27.709898698468745	22.66150458824937
80-81	22.376988480803696	27.413818186226667	27.658416731829632	22.55077660114
82-83	22.41046022255936	27.38485657681497	27.725017038645188	22.479666161980482
84-85	22.427235365258568	27.46108509330047	27.640388835416886	22.471290706024078
86-87	22.409269279189644	27.422468032223918	27.719438022978366	22.448824665608072
88-89	22.416908072454838	27.407162640903802	27.733803130652458	22.4421261559889
90-91	22.48218177339951	27.413557365879065	27.695959247018436	22.408301613702985
92-93	22.559278362032387	27.431404945863314	27.655721603780385	22.35359508832391
94-95	22.399736862573114	27.44825868077909	27.690965811088763	22.46103864555903
96-97	22.462591875862998	27.469472130401783	27.687876263488885	22.380059730246334
98-99	22.65885763220555	28.025634851476305	27.788431242768606	21.527076273549536
100-101	23.296758042271566	30.49139407596375	25.139780662691148	21.07206721907353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3666.0
1	3331.5
2	3640.0
3	4488.0
4	5397.0
5	7449.5
6	9689.0
7	13793.5
8	18021.0
9	19220.0
10	18067.5
11	16509.5
12	17468.5
13	21434.5
14	30145.5
15	41155.0
16	49295.0
17	53183.5
18	52878.0
19	49305.5
20	44329.0
21	40559.5
22	40171.5
23	44841.0
24	54627.5
25	69552.5
26	88963.5
27	110294.5
28	134988.0
29	163045.0
30	190488.5
31	218784.0
32	248903.5
33	279682.5
34	307425.5
35	331881.5
36	361317.5
37	387535.5
38	406935.5
39	425215.5
40	440060.5
41	453118.5
42	460636.0
43	466987.0
44	476798.5
45	486414.0
46	489641.5
47	487211.5
48	485914.5
49	484121.0
50	479416.0
51	471193.5
52	457146.5
53	437041.0
54	414625.5
55	388790.5
56	359556.5
57	327779.5
58	295194.0
59	256487.5
60	213647.0
61	174329.5
62	139163.0
63	108822.0
64	82262.0
65	61292.5
66	45822.5
67	35218.5
68	27347.0
69	20718.0
70	15414.5
71	11113.0
72	7864.5
73	5369.5
74	3542.5
75	2240.5
76	1412.0
77	874.0
78	530.0
79	332.5
80	226.5
81	151.0
82	105.0
83	73.5
84	46.0
85	27.0
86	17.0
87	11.5
88	11.0
89	9.0
90	6.5
91	4.0
92	2.5
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003206467703541156
4	0.0
5	0.04799707370261736
6	0.0
7	0.0026817729884162397
8	0.0
9	0.0
10-11	8.328487541665341E-6
12-13	0.0
14-15	1.6656975083330683E-5
16-17	0.0
18-19	0.0
20-21	7.079350903477173E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.297084323062717E-5
46-47	8.288988698007536E-4
48-49	1.8485808906647589E-4
50-51	7.81004306750624E-4
52-53	0.0014349503480358078
54-55	0.0031601043932833646
56-57	0.0028075020991634235
58-59	0.0065037787731651985
60-61	0.0058758135063799585
62-63	0.0066386613336861565
64-65	0.0038359362119872306
66-67	0.006402543250833452
68-69	0.00666732513258701
70-71	0.0032546991283424636
72-73	0.0026776052917255825
74-75	0.003609065442801192
76-77	0.0022917224026259278
78-79	0.0
80-81	5.404961158282166E-4
82-83	1.8373224495795337E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.6610048997615418E-5
92-93	7.21426024517825E-4
94-95	8.714595051286481E-5
96-97	5.51943697327323E-6
98-99	1.139306348140917E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	126.0
20-21	603.0
22-23	1179.0
24-25	1536.0
26-27	3572.0
28-29	8585.0
30-31	23935.0
32-33	30861.0
34-35	42578.0
36-37	65951.0
38-39	74692.0
40-41	76393.0
42-43	87365.0
44-45	102474.0
46-47	102708.0
48-49	95144.0
50-51	85434.0
52-53	76387.0
54-55	82258.0
56-57	82810.0
58-59	90424.0
60-61	88131.0
62-63	85865.0
64-65	92032.0
66-67	97196.0
68-69	99062.0
70-71	97291.0
72-73	92711.0
74-75	93799.0
76-77	98510.0
78-79	103101.0
80-81	102872.0
82-83	96947.0
84-85	96599.0
86-87	100765.0
88-89	105100.0
90-91	108350.0
92-93	106273.0
94-95	113884.0
96-97	180415.0
98-99	1002556.0
100-101	7910508.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59203396481884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55353795723772	73.13816814669053
2	7.703305968645576	12.72463416407534
3	2.010715151673038	4.982071623016662
4	0.7598633846799017	2.5103464990441857
5	0.3665386086321687	1.5136584606782757
6	0.18534352238793603	0.9184739097734145
7	0.11249699888753065	0.6503949167141384
8	0.07781450824532547	0.5141486806362878
9	0.049266443414558415	0.3662114191038931
>10	0.17581455182767444	2.2478627568776437
>50	0.0038731379305668675	0.21644832501129976
>100	0.001372650034817472	0.17410283176060368
>500	3.807771684065684E-5	0.026165660064327613
>1k	1.9038686337685093E-5	0.01731260655341029
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.328487541665341E-6
2	0.0	2.4985462624996022E-5	0.0	0.0	8.328487541665341E-6
3	0.0	2.4985462624996022E-5	0.0	0.0	8.328487541665341E-6
4	0.0	4.9970925249992045E-5	0.0	1.6656975083330683E-5	2.4985462624996022E-5
5	0.0	5.829941279165739E-5	0.0	2.4985462624996022E-5	2.4985462624996022E-5
6	8.328487541665341E-6	5.829941279165739E-5	0.0	2.4985462624996022E-5	2.4985462624996022E-5
7	8.328487541665341E-6	5.829941279165739E-5	0.0	2.4985462624996022E-5	2.4985462624996022E-5
8	8.328487541665341E-6	7.495638787498807E-5	0.0	2.4985462624996022E-5	2.4985462624996022E-5
9	8.328487541665341E-6	1.9988370099996818E-4	0.0	4.16424377083267E-5	2.4985462624996022E-5
10-11	8.328487541665341E-6	1.9988370099996818E-4	0.0	5.4135169020824717E-5	2.4985462624996022E-5
12-13	8.328487541665341E-6	2.0821218854163352E-4	0.0	6.246365656249006E-5	2.4985462624996022E-5
14-15	8.328487541665341E-6	2.7900433264578897E-4	0.0	6.662790033332273E-5	3.331395016666136E-5
16-17	4.16424377083267E-5	2.831685764166216E-4	0.0	8.744911918748608E-5	4.9970925249992045E-5
18-19	4.16424377083267E-5	3.248110141249483E-4	0.0	9.994185049998409E-5	7.07921441041554E-5
20-21	4.16424377083267E-5	3.5812496429160964E-4	0.0	1.0410609427081676E-4	7.495638787498807E-5
22-23	4.16424377083267E-5	3.9976740199993636E-4	0.0	1.290915568958128E-4	8.32848754166534E-5
24-25	4.16424377083267E-5	4.497383272499284E-4	0.0	1.6240550706247416E-4	9.577760672915142E-5
26-27	4.16424377083267E-5	4.663953023332591E-4	0.0	2.8316857641662156E-4	9.994185049998409E-5
28-29	4.16424377083267E-5	4.663953023332591E-4	0.0	7.662208538332113E-4	9.994185049998409E-5
30-31	4.16424377083267E-5	5.246947151249165E-4	0.0	0.0022070491985413153	9.994185049998409E-5
32-33	4.16424377083267E-5	5.246947151249165E-4	0.0	0.005859090985561567	1.2492731312498012E-4
34-35	4.16424377083267E-5	6.121438343124026E-4	0.0	0.010693778003498296	1.2492731312498012E-4
36-37	4.16424377083267E-5	6.95428709729056E-4	0.0	0.017648065100788857	1.2492731312498012E-4
38-39	4.16424377083267E-5	7.287426598957174E-4	0.0	0.030594698984307632	1.2492731312498012E-4
40-41	4.16424377083267E-5	7.870420726873747E-4	0.0	0.06475399063644803	1.2492731312498012E-4
42-43	4.16424377083267E-5	8.203560228540361E-4	0.0	0.07741329169977934	1.290915568958128E-4
44-45	4.16424377083267E-5	9.119693858123548E-4	0.0	0.09415355165852668	1.3325580066664546E-4
46-47	4.16424377083267E-5	9.910900174581756E-4	0.0	0.11315499598483617	1.3742004443747813E-4
48-49	4.16424377083267E-5	9.99418504999841E-4	0.0	0.13022006695770844	1.415842882083108E-4
50-51	4.16424377083267E-5	0.0011410027932081518	0.0	0.1473101233932057	1.415842882083108E-4
52-53	4.16424377083267E-5	0.0011618240120623152	0.0	0.17341576759255573	1.4991277574997615E-4
54-55	4.16424377083267E-5	0.0011784809871456458	0.0	0.23379730226962944	1.582412632916415E-4
56-57	4.16424377083267E-5	0.0012159591810831398	0.0	0.29942578409795234	1.582412632916415E-4
58-59	4.16424377083267E-5	0.0012409446437081357	0.0	0.3717920123474825	1.665697508333068E-4
60-61	4.16424377083267E-5	0.0012659301063331317	0.0	0.3923633765753959	1.832267259166375E-4
62-63	4.16424377083267E-5	0.001286751325187295	0.0	0.40876216854493497	1.9988370099996818E-4
64-65	4.16424377083267E-5	0.0014907992699580961	0.0	0.4175862010953294	2.0821218854163352E-4
66-67	4.16424377083267E-5	0.0015074562450414267	0.0	0.4232828865738285	2.0821218854163352E-4
68-69	4.16424377083267E-5	0.00152827746389559	0.0	0.42926274062874414	2.0821218854163352E-4
70-71	4.5806681479159373E-5	0.0015324417076664227	0.0	0.43494693337593077	2.0821218854163352E-4
72-73	6.662790033332273E-5	0.0015324417076664227	0.0	0.4395067803049926	2.1654067608329887E-4
74-75	6.662790033332273E-5	0.0015615914140622515	0.0	0.4427382334711587	2.1654067608329887E-4
76-77	6.662790033332273E-5	0.0016532047770205702	0.0	0.4458281023491165	2.1654067608329887E-4
78-79	6.662790033332273E-5	0.0016906829709580642	0.0	0.44873890874492856	2.1654067608329887E-4
80-81	6.662790033332273E-5	0.0016906829709580642	0.0	0.45002149582634504	2.248691636249642E-4
82-83	6.662790033332273E-5	0.0016906829709580642	0.0	0.4505003838599908	2.3319765116662955E-4
84-85	7.07921441041554E-5	0.0016948472147288967	0.0	0.4509209724808449	2.3319765116662955E-4
86-87	7.495638787498807E-5	0.0017156684335830604	0.0	0.451066721012824	2.3319765116662955E-4
88-89	7.495638787498807E-5	0.0017614751150622195	0.0	0.45109587071921986	2.415261387082949E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16775	0.0	50.976418	1
GTATCAA	30095	0.0	37.1506	1
TCAACGC	38205	0.0	29.20753	4
ATCAACG	38920	0.0	28.66036	3
CAACGCA	39150	0.0	28.50252	5
TATCAAC	39855	0.0	28.052874	2
AACGCAG	40425	0.0	27.685322	6
TGGTATC	7795	0.0	25.932652	2
GTGGTAT	8230	0.0	25.714132	1
ACGCAGA	46205	0.0	24.194065	7
CGCAGAG	47700	0.0	23.462679	8
GCAGAGT	53625	0.0	20.854233	9
GAGTACT	31375	0.0	19.43493	12-13
GTACATG	38395	0.0	19.364426	1
AGAGTAC	49065	0.0	19.30241	10-11
TACATGG	39560	0.0	18.837746	2
CAGAGTA	52775	0.0	18.569918	10-11
ACATGGG	39180	0.0	18.316395	3
GTACTTT	34240	0.0	17.764843	14-15
AGTACTT	34465	0.0	17.661222	12-13
>>END_MODULE