FastQCFastQC Report
Fri 27 May 2016
SRR2139500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10776029
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158350.051.582211
GTATCAA283150.036.542651
CTTATAC142000.030.2040651
ATCAACG356650.028.84243
TCAACGC357350.028.8332614
TATCAAC367650.028.214172
CAACGCA365400.028.1390065
AACGCAG375650.027.4634866
TATACAC186350.025.8174213
GTGGTAT75400.024.7790091
TGGTATC73900.024.1725962
ACGCAGA426600.024.1025687
CGCAGAG436200.023.6706588
TTATACA189700.023.3371282
GCAGAGT494550.020.8516889
TATGCCG127400.020.04311842-43
ACATCTC220900.020.0319618
GAGTACT298750.019.96577512-13
ACACATC220200.019.9000556
TACACAT237450.019.8973485
Produced by FastQC (version 0.11.5)