##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139500_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10776029
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.573100721982097	31.0	18.0	32.0	18.0	33.0
2	30.74897237191919	33.0	30.0	33.0	27.0	33.0
3	31.20930947754502	33.0	31.0	33.0	27.0	34.0
4	31.755717899422876	33.0	32.0	33.0	29.0	34.0
5	32.123767855487394	33.0	33.0	34.0	31.0	34.0
6	35.44653962976529	38.0	36.0	38.0	29.0	38.0
7	35.73524412378623	38.0	36.0	38.0	31.0	38.0
8	36.01501786975518	38.0	37.0	38.0	31.0	38.0
9	36.52154388225941	38.0	37.0	38.0	34.0	38.0
10-11	36.62157006073387	38.0	38.0	38.0	34.0	38.0
12-13	36.692869887414	38.0	38.0	38.0	34.0	38.0
14-15	36.683900535160035	38.0	38.0	38.0	34.0	38.0
16-17	36.6979585893839	38.0	38.0	38.0	34.0	38.0
18-19	36.73406688122313	38.0	38.0	38.0	34.0	38.0
20-21	36.75983169331943	38.0	38.0	38.0	34.5	38.0
22-23	36.78586213451334	38.0	38.0	38.0	35.0	38.0
24-25	36.78020856247702	38.0	38.0	38.0	35.0	38.0
26-27	36.74571592517597	38.0	38.0	38.0	34.5	38.0
28-29	36.741356763509565	38.0	38.0	38.0	34.5	38.0
30-31	36.7199941408206	38.0	38.0	38.0	34.0	38.0
32-33	36.69677932150388	38.0	38.0	38.0	34.0	38.0
34-35	36.662377059843614	38.0	38.0	38.0	34.0	38.0
36-37	36.63507227582704	38.0	38.0	38.0	34.0	38.0
38-39	36.598040298668224	38.0	38.0	38.0	34.0	38.0
40-41	36.62368169202442	38.0	38.0	38.0	34.0	38.0
42-43	36.59706519640468	38.0	38.0	38.0	34.0	38.0
44-45	36.548062421137814	38.0	38.0	38.0	34.0	38.0
46-47	36.501167367433226	38.0	38.0	38.0	34.0	38.0
48-49	36.45594156987455	38.0	38.0	38.0	34.0	38.0
50-51	36.43251689546845	38.0	38.0	38.0	34.0	38.0
52-53	36.3972217393501	38.0	38.0	38.0	34.0	38.0
54-55	36.325758970491776	38.0	38.0	38.0	33.0	38.0
56-57	36.33373189008276	38.0	38.0	38.0	33.0	38.0
58-59	36.321706690769176	38.0	38.0	38.0	33.0	38.0
60-61	36.276879519293836	38.0	38.0	38.0	33.0	38.0
62-63	36.280263699175485	38.0	38.0	38.0	33.0	38.0
64-65	36.30014594912869	38.0	38.0	38.0	33.0	38.0
66-67	36.275051208275954	38.0	38.0	38.0	33.0	38.0
68-69	36.25737162755691	38.0	37.0	38.0	33.0	38.0
70-71	36.25485257991839	38.0	37.0	38.0	33.0	38.0
72-73	36.23882700117197	38.0	37.0	38.0	33.0	38.0
74-75	36.226845854581896	38.0	37.0	38.0	33.0	38.0
76-77	36.191551122193395	38.0	37.0	38.0	33.0	38.0
78-79	36.18875976162877	38.0	37.0	38.0	33.0	38.0
80-81	36.181638132864975	38.0	37.0	38.0	33.0	38.0
82-83	36.157129431403476	38.0	37.0	38.0	33.0	38.0
84-85	36.11496959089192	38.0	37.0	38.0	33.0	38.0
86-87	36.1284631742581	38.0	37.0	38.0	33.0	38.0
88-89	36.129678347764255	38.0	37.0	38.0	33.0	38.0
90-91	36.12116637739126	38.0	37.0	38.0	33.0	38.0
92-93	36.11259883619438	38.0	37.0	38.0	33.0	38.0
94-95	36.09605970948406	38.0	37.0	38.0	33.0	38.0
96-97	36.091926589412225	38.0	37.0	38.0	33.0	38.0
98-99	36.140666338386346	38.0	37.0	38.0	33.0	38.0
100-101	35.61572771684064	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	19.0
19	46.0
20	149.0
21	626.0
22	2669.0
23	7767.0
24	17588.0
25	32822.0
26	53794.0
27	80023.0
28	113678.0
29	150556.0
30	193821.0
31	243703.0
32	309315.0
33	401120.0
34	551795.0
35	854389.0
36	1815051.0
37	5947093.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.89012093415859	20.071280431780576	16.05389146595652	23.984707168104315
2	14.432923296698627	21.450239230054038	41.39478466511179	22.72205280813554
3	18.162220110669963	25.75503846963007	31.169360380628795	24.913381039071172
4	12.743943061029253	18.566728059102292	37.44943522330907	31.239893656559385
5	13.76639099786227	36.99990947726603	35.25814994742718	13.975549577444522
6	30.1115373761522	37.79280846404552	18.19002157473778	13.905632585064499
7	26.53260103248814	32.55895640169466	22.346837802069228	18.561604763747972
8	24.253683801333498	37.16036770131186	20.58640525187896	17.999543245475675
9	25.066524969448395	17.21577586697289	20.99163801433719	36.726061149241524
10-11	24.342037312631582	26.840383410252517	28.31997760956286	20.49760166755305
12-13	25.194610185254696	24.128948613631238	28.599547198694435	22.076894002419632
14-15	22.230224553075082	25.595555764661686	26.228015204490486	25.946204477772746
16-17	21.807031142919158	28.83623921205112	26.952284556769474	22.40444508826025
18-19	21.863958421047307	27.661033577396644	28.93695349186607	21.53805450968998
20-21	22.53039059899406	26.70441674632832	29.002235846805753	21.762956807871873
22-23	22.30741641953188	26.897875223184652	28.809097325886274	21.985611031397198
24-25	22.15287532046573	27.11305036777738	28.895935527878674	21.838138783878218
26-27	22.069777393385593	27.302633976125755	28.85817836072824	21.769410269760414
28-29	21.960455539994875	27.195083897666024	28.893890905173535	21.95056965716557
30-31	22.42544563067348	26.97439833108051	28.863780172917448	21.736375865328565
32-33	22.06624774841881	27.16924034250439	28.847971403294785	21.91654050578201
34-35	22.154013701190554	26.920985275074628	29.008638547554455	21.916362476180364
36-37	21.95028499104951	27.1868481635694	28.92460521086178	21.938261634519304
38-39	21.891514507524214	26.947858100634186	29.123531851295414	22.037095540546183
40-41	22.126150056377195	26.811991703082573	29.056884300945065	22.00497393959517
42-43	22.198427632333374	26.851265196828468	28.892445563950314	22.057861606887847
44-45	22.064049307815726	27.115201973789738	28.725012684307117	22.095736034087416
46-47	22.348546580322523	26.869231225910696	28.285550509889106	22.49667168387768
48-49	22.218890787953182	26.96502033153299	28.315865561902402	22.500223318611425
50-51	22.06387486142278	27.120536534124717	28.400625976856332	22.41496262759618
52-53	22.212173938944144	27.117924329261783	28.454088965118384	22.215812766675686
54-55	22.198548480605847	27.08951362737033	28.21942870072334	22.492509191300478
56-57	22.39784212838899	27.32238652395128	27.935148304606017	22.344623043053712
58-59	22.05253711332294	27.554427618438044	28.32553856304984	22.06749670518918
60-61	22.354140865225954	27.811013050804927	27.85093795682888	21.983908127140232
62-63	22.080913959873914	28.154453986175547	27.77522017382151	21.98941188012903
64-65	22.008280571552184	28.026872602301705	27.813222308152202	22.151624517993906
66-67	22.0810874661606	28.038672309633494	27.68119131878467	22.199048905421233
68-69	22.04588113490313	27.82833862356595	27.823665082837977	22.302115158692935
70-71	22.162841058080904	27.426729606758073	27.927258023304567	22.483171311856452
72-73	22.253247802710394	27.29841425860984	27.929767785106396	22.51857015357337
74-75	22.17851407113749	27.41834995873364	27.917562802164813	22.485573167964056
76-77	22.360568760810132	27.324171948465235	27.80316603001675	22.51209326070788
78-79	22.287829410178244	27.34514941833878	27.74121398903782	22.62580718244515
80-81	22.317665365492985	27.396105475190787	27.746311896532095	22.539917262784137
82-83	22.375170114927386	27.37021119806568	27.815695937300028	22.438922749706904
84-85	22.44324534711731	27.41027087143028	27.72943086059551	22.4170529208569
86-87	22.348072797185335	27.472385833952938	27.740665774079375	22.438875594782353
88-89	22.38865166235232	27.45948662532811	27.715193981814508	22.436667730505064
90-91	22.409788497728464	27.49398806870829	27.72029872296553	22.375924710597708
92-93	22.517689397702977	27.44312644861881	27.71874050567149	22.320443648006723
94-95	22.373194116141647	27.482879685587598	27.74343396087148	22.400492237399273
96-97	22.4043244255745	27.475255368495393	27.743935272139613	22.37648493379049
98-99	22.54355103156169	28.06860505128711	27.845074716744346	21.542769200406855
100-101	23.23208412767507	30.56598984843268	25.14926656917531	21.05265945471694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3395.0
1	3103.0
2	3345.0
3	4109.0
4	5063.5
5	7054.0
6	9124.5
7	12698.0
8	16535.0
9	17964.5
10	17040.5
11	15569.0
12	16442.0
13	20324.0
14	29155.5
15	40321.0
16	48201.0
17	51770.5
18	51357.5
19	47705.5
20	42612.0
21	38007.5
22	36322.0
23	39144.0
24	46705.5
25	59243.0
26	75621.0
27	93962.0
28	115271.0
29	139219.5
30	162596.5
31	186688.0
32	212508.5
33	238520.5
34	263042.5
35	285382.0
36	310042.0
37	334093.5
38	353586.5
39	369371.5
40	384515.5
41	400036.5
42	410195.5
43	424429.5
44	459069.0
45	479222.0
46	471480.0
47	462801.5
48	454451.5
49	448299.0
50	442288.5
51	433148.0
52	416651.5
53	393653.5
54	369384.0
55	343290.0
56	316861.0
57	287997.5
58	256900.0
59	221709.0
60	183250.0
61	148451.5
62	116849.5
63	89604.5
64	67347.5
65	49895.5
66	37003.0
67	27878.0
68	21223.5
69	15880.5
70	11792.5
71	8497.0
72	5974.5
73	4066.0
74	2591.0
75	1653.0
76	1082.0
77	673.5
78	425.5
79	279.0
80	166.0
81	86.0
82	51.5
83	39.5
84	30.0
85	20.5
86	15.5
87	12.5
88	11.0
89	10.0
90	7.0
91	4.0
92	2.5
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003582024510141909
4	0.0
5	0.04875636470540307
6	0.0
7	0.002895315148093978
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.8559712487781908E-5
16-17	0.0
18-19	0.0
20-21	5.5680377557504137E-5
22-23	9.280749780637878E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.808304210636805E-6
46-47	8.003027763667388E-4
48-49	1.8100270902351827E-4
50-51	7.148504813803141E-4
52-53	0.0013651622991815231
54-55	0.0030381063483130967
56-57	0.0028332384055882935
58-59	0.006457908931115705
60-61	0.005701773052163713
62-63	0.006622608297283634
64-65	0.0038786414121245497
66-67	0.006453671732912567
68-69	0.006623060085831679
70-71	0.0031837443373971454
72-73	0.0025842945970147445
74-75	0.003530044035386779
76-77	0.0022054678952519637
78-79	0.0
80-81	5.341483875465834E-4
82-83	2.1012315431654578E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	5.682731769317939E-4
94-95	8.470154563380473E-5
96-97	0.0
98-99	1.2650987959928247E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	135.0
20-21	615.0
22-23	1246.0
24-25	1482.0
26-27	3191.0
28-29	7156.0
30-31	19679.0
32-33	26093.0
34-35	36508.0
36-37	56074.0
38-39	63930.0
40-41	64915.0
42-43	74565.0
44-45	88956.0
46-47	89577.0
48-49	81462.0
50-51	72552.0
52-53	64535.0
54-55	69711.0
56-57	70580.0
58-59	76980.0
60-61	77722.0
62-63	77499.0
64-65	91469.0
66-67	99312.0
68-69	97046.0
70-71	114330.0
72-73	79057.0
74-75	80313.0
76-77	84752.0
78-79	89327.0
80-81	89429.0
82-83	85043.0
84-85	83935.0
86-87	87751.0
88-89	92534.0
90-91	94618.0
92-93	93624.0
94-95	100464.0
96-97	161112.0
98-99	905572.0
100-101	7121178.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.49522588467005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.37429490388052	72.90457420494069
2	7.854165111697266	12.958622500499214
3	2.0346758158711316	5.035531230970929
4	0.7660595559294167	2.52785024430029
5	0.3633730957316537	1.4988272806397303
6	0.19950107095986977	0.9874731547840828
7	0.11160671007668242	0.6444914530614558
8	0.07076013760467385	0.4669898828262324
9	0.04728964354715855	0.35110528437855365
>10	0.17365149631757137	2.257233049744912
>50	0.003494485007410833	0.1869886587531693
>100	0.0010865582210237248	0.15180195712033878
>500	2.0707691930644627E-5	0.011206354345298594
>1k	2.0707463681458233E-5	0.017304743635212047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.855971248778191E-5
2	0.0	9.279856243890955E-6	0.0	0.0	1.855971248778191E-5
3	0.0	9.279856243890955E-6	0.0	0.0	1.855971248778191E-5
4	0.0	8.35187061950186E-5	0.0	0.0	1.855971248778191E-5
5	0.0	8.35187061950186E-5	0.0	0.0	1.855971248778191E-5
6	0.0	8.35187061950186E-5	0.0	0.0	2.7839568731672863E-5
7	0.0	1.020784186828005E-4	0.0	9.279856243890955E-6	2.7839568731672863E-5
8	0.0	1.020784186828005E-4	0.0	9.279856243890955E-6	2.7839568731672863E-5
9	0.0	2.04156837365601E-4	0.0	9.279856243890955E-6	2.7839568731672863E-5
10-11	0.0	2.04156837365601E-4	0.0	1.855971248778191E-5	2.7839568731672863E-5
12-13	0.0	2.04156837365601E-4	0.0	1.855971248778191E-5	2.7839568731672863E-5
14-15	0.0	2.3663633421921933E-4	0.0	2.3199640609727387E-5	4.6399281219454774E-5
16-17	0.0	2.5055611858505576E-4	0.0	3.2479496853618346E-5	5.5679137463345726E-5
18-19	0.0	2.644759029508922E-4	0.0	3.711942497556382E-5	5.5679137463345726E-5
20-21	0.0	2.783956873167286E-4	0.0	4.17593530975093E-5	5.5679137463345726E-5
22-23	0.0	3.10875184170347E-4	0.0	4.6399281219454774E-5	6.495899370723669E-5
24-25	0.0	3.4335468102396534E-4	0.0	5.5679137463345726E-5	7.423884995112764E-5
26-27	0.0	3.526345372678563E-4	0.0	1.3455791553641886E-4	7.423884995112764E-5
28-29	0.0	3.5727446538980176E-4	0.0	4.454330997067658E-4	8.35187061950186E-5
30-31	0.0	4.315133153409294E-4	0.0	0.0017167734051198266	8.35187061950186E-5
32-33	0.0	4.4079317158482033E-4	0.0	0.0044311313564579305	9.279856243890955E-5
34-35	0.0	5.05752165292057E-4	0.0	0.008119874213404585	9.279856243890955E-5
36-37	0.0	5.707111589992937E-4	0.0	0.013567149828568575	9.279856243890955E-5
38-39	0.0	5.939107996090212E-4	0.0	0.024605538830676864	9.279856243890955E-5
40-41	0.0	6.681496495601488E-4	0.0	0.05534042271044371	9.279856243890955E-5
42-43	0.0	6.774295058040397E-4	0.0	0.06582666026604049	9.279856243890955E-5
44-45	0.0	7.331086432673854E-4	0.0	0.08052131262824182	9.743849056085502E-5
46-47	0.0	7.795079244868402E-4	0.0	0.09607435169300305	1.020784186828005E-4
48-49	0.0	7.841478526087857E-4	0.0	0.11037925009296096	1.1135827492669145E-4
50-51	0.0	9.001460556574226E-4	0.0	0.12461918949921164	1.1135827492669145E-4
52-53	0.0	9.187057681452045E-4	0.0	0.1475404344216223	1.206381311705824E-4
54-55	0.0	9.279856243890954E-4	0.0	0.20256998194789566	1.3919784365836434E-4
56-57	0.0	9.279856243890954E-4	0.0	0.2638866320794051	1.484776999022553E-4
58-59	0.0	9.372654806329864E-4	0.0	0.3348218532077076	1.484776999022553E-4
60-61	0.0	9.465453368768773E-4	0.0	0.35321452828309946	1.484776999022553E-4
62-63	0.0	9.465453368768773E-4	0.0	0.36421115793211023	1.7631726863392814E-4
64-65	0.0	0.0010486237555596778	0.0	0.3723403120017587	1.7631726863392814E-4
66-67	9.279856243890955E-6	0.0010486237555596778	0.0	0.37805206351987364	1.7631726863392814E-4
68-69	9.279856243890955E-6	0.0010579036118035688	0.0	0.38329054236955007	1.8095719675587362E-4
70-71	9.279856243890955E-6	0.0010718233961694052	0.0	0.38787943128215413	1.9487698112171005E-4
72-73	9.279856243890955E-6	0.0010811032524132961	0.0	0.39183728997017364	2.3199640609727386E-4
74-75	1.3919784365836433E-5	0.001099662964901078	0.0	0.3951409187929988	2.3199640609727386E-4
76-77	1.855971248778191E-5	0.001127502533632751	0.0	0.3981243925754097	2.3199640609727386E-4
78-79	1.855971248778191E-5	0.0011507021742424783	0.0	0.40065779332999196	2.3663633421921933E-4
80-81	1.855971248778191E-5	0.0011553421023644238	0.0	0.40166465773245413	2.459161904631103E-4
82-83	1.855971248778191E-5	0.0011599820304863692	0.0	0.4020683314790634	2.598359748289467E-4
84-85	1.855971248778191E-5	0.0011692618867302602	0.0	0.40242096601633126	2.598359748289467E-4
86-87	1.855971248778191E-5	0.0011692618867302602	0.0	0.4025601638599896	2.598359748289467E-4
88-89	1.855971248778191E-5	0.0011971014554619333	0.0	0.4025972832849652	2.598359748289467E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15835	0.0	51.58221	1
GTATCAA	28315	0.0	36.54265	1
CTTATAC	14200	0.0	30.204065	1
ATCAACG	35665	0.0	28.8424	3
TCAACGC	35735	0.0	28.833261	4
TATCAAC	36765	0.0	28.21417	2
CAACGCA	36540	0.0	28.139006	5
AACGCAG	37565	0.0	27.463486	6
TATACAC	18635	0.0	25.817421	3
GTGGTAT	7540	0.0	24.779009	1
TGGTATC	7390	0.0	24.172596	2
ACGCAGA	42660	0.0	24.102568	7
CGCAGAG	43620	0.0	23.670658	8
TTATACA	18970	0.0	23.337128	2
GCAGAGT	49455	0.0	20.851688	9
TATGCCG	12740	0.0	20.043118	42-43
ACATCTC	22090	0.0	20.031961	8
GAGTACT	29875	0.0	19.965775	12-13
ACACATC	22020	0.0	19.900055	6
TACACAT	23745	0.0	19.897348	5
>>END_MODULE