FastQCFastQC Report
Fri 27 May 2016
SRR2139499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893248
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195400.052.6790051
GTATCAA350550.036.6553041
TCAACGC436950.029.3176064
ATCAACG439150.029.1810513
CAACGCA447800.028.6266025
TATCAAC451700.028.5333372
AACGCAG462800.027.7314826
GTGGTAT88350.026.773291
TGGTATC88200.025.44352
ACGCAGA526100.024.3948487
CGCAGAG540600.023.7954888
GAGACCG111600.022.39126288-89
CGAGACC109100.022.34007888-89
AGACCGA110150.022.15982890-91
GACCGAG114700.021.97283690-91
ACCGAGG118700.021.21648692-93
GCAGAGT607000.021.1996239
ATCTCCG111450.020.95721674-75
ACGAGAC120200.020.73649886-87
GAGTACT373600.020.30463812-13
Produced by FastQC (version 0.11.5)