##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12893248
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.13380980494597	31.0	25.0	33.0	18.0	33.0
2	30.905204801769113	33.0	31.0	33.0	27.0	33.0
3	31.285309489121747	33.0	31.0	33.0	28.0	34.0
4	31.77334035613059	33.0	32.0	33.0	29.0	34.0
5	32.1334679981336	33.0	33.0	34.0	31.0	34.0
6	35.48454136614762	38.0	36.0	38.0	29.0	38.0
7	35.77717554180297	38.0	36.0	38.0	31.0	38.0
8	36.053938154295956	38.0	37.0	38.0	31.0	38.0
9	36.52375181180103	38.0	37.0	38.0	34.0	38.0
10-11	36.616709148850624	38.0	38.0	38.0	34.0	38.0
12-13	36.686315930632844	38.0	38.0	38.0	34.0	38.0
14-15	36.67465711510397	38.0	38.0	38.0	34.0	38.0
16-17	36.69445720736931	38.0	38.0	38.0	34.0	38.0
18-19	36.727835724559085	38.0	38.0	38.0	34.0	38.0
20-21	36.75071674186816	38.0	38.0	38.0	34.0	38.0
22-23	36.77528669817274	38.0	38.0	38.0	35.0	38.0
24-25	36.77040781444471	38.0	38.0	38.0	35.0	38.0
26-27	36.73579212296829	38.0	38.0	38.0	34.0	38.0
28-29	36.729262492535014	38.0	38.0	38.0	34.0	38.0
30-31	36.7088585035431	38.0	38.0	38.0	34.0	38.0
32-33	36.691684140201424	38.0	38.0	38.0	34.0	38.0
34-35	36.6631760906341	38.0	38.0	38.0	34.0	38.0
36-37	36.638921367657275	38.0	38.0	38.0	34.0	38.0
38-39	36.62866358369018	38.0	38.0	38.0	34.0	38.0
40-41	36.61238399851531	38.0	38.0	38.0	34.0	38.0
42-43	36.57242746735928	38.0	38.0	38.0	34.0	38.0
44-45	36.523864971566994	38.0	38.0	38.0	34.0	38.0
46-47	36.477079086923275	38.0	38.0	38.0	34.0	38.0
48-49	36.43662767186095	38.0	38.0	38.0	34.0	38.0
50-51	36.41235696371215	38.0	38.0	38.0	34.0	38.0
52-53	36.37772594349101	38.0	38.0	38.0	34.0	38.0
54-55	36.30575262942899	38.0	38.0	38.0	33.0	38.0
56-57	36.312781644318164	38.0	38.0	38.0	33.0	38.0
58-59	36.31033281992145	38.0	38.0	38.0	33.0	38.0
60-61	36.268447773201046	38.0	38.0	38.0	33.0	38.0
62-63	36.27396796030145	38.0	38.0	38.0	33.0	38.0
64-65	36.29410251849873	38.0	38.0	38.0	33.0	38.0
66-67	36.27493460949715	38.0	37.5	38.0	33.0	38.0
68-69	36.26188383025165	38.0	37.0	38.0	33.0	38.0
70-71	36.26506053701165	38.0	37.0	38.0	33.0	38.0
72-73	36.252678159009484	38.0	37.0	38.0	33.0	38.0
74-75	36.23765316780403	38.0	37.0	38.0	33.0	38.0
76-77	36.198972901822245	38.0	37.0	38.0	33.0	38.0
78-79	36.19628938336933	38.0	37.0	38.0	33.0	38.0
80-81	36.186983819578984	38.0	37.0	38.0	33.0	38.0
82-83	36.153246731351196	38.0	37.0	38.0	33.0	38.0
84-85	36.110662491571716	38.0	37.0	38.0	33.0	38.0
86-87	36.12563771795905	38.0	37.0	38.0	33.0	38.0
88-89	36.124045929735686	38.0	37.0	38.0	33.0	38.0
90-91	36.111222081387595	38.0	37.0	38.0	33.0	38.0
92-93	36.100488300713685	38.0	37.0	38.0	33.0	38.0
94-95	36.08409294868213	38.0	37.0	38.0	33.0	38.0
96-97	36.07198495585805	38.0	37.0	38.0	33.0	38.0
98-99	36.12091913095929	38.0	37.0	38.0	33.0	38.0
100-101	35.59575706419889	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	15.0
19	22.0
20	133.0
21	725.0
22	3089.0
23	9335.0
24	20926.0
25	38736.0
26	64335.0
27	96955.0
28	136163.0
29	181411.0
30	234093.0
31	296222.0
32	374442.0
33	486064.0
34	663406.0
35	1018206.0
36	2115617.0
37	7153349.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.53375844473014	19.958143983579625	15.927933752612219	23.580163819078017
2	14.586076371136272	21.495111239619373	41.160846359272696	22.75796602997166
3	18.318546044584473	25.696181521046967	30.93710224985515	25.048170184513406
4	12.87468448601935	18.165314124105887	37.74380202723162	31.21619936264314
5	13.81234444588086	37.09474839516492	35.04716460642412	14.045742552530093
6	30.328533198151465	37.70333123197506	18.206413155164626	13.761722414708846
7	26.74403452948856	32.64732335654824	22.532906575296227	18.07573553866697
8	24.441940463721785	37.04384651563361	20.46831799093603	18.04589502970857
9	25.276726236864445	17.246135341536903	21.235176737467548	36.241961684131105
10-11	24.448491351771537	26.899623233014026	28.222664615722298	20.429220799492136
12-13	25.19958120715587	24.3741879470557	28.658860823897903	21.767370021890528
14-15	22.249685348861874	25.732155533351857	26.533408282125976	25.484750835660297
16-17	21.668174691125152	28.819107489439432	27.256673415418675	22.25604440401674
18-19	21.690612792059845	27.80482078681803	29.29857162446577	21.20599479665636
20-21	22.504910134218413	26.844322810615314	29.35485616103805	21.295910894128216
22-23	22.29620112239275	26.696900476059554	29.212311066324304	21.79458733522339
24-25	22.018666501071127	26.863464707302192	29.284985280058994	21.832883511567687
26-27	21.915610387010727	27.002185620217254	29.238070447264636	21.844133545507376
28-29	21.856928270405056	26.969327845657926	29.31396709738766	21.859776786549354
30-31	22.08655260256796	26.91462905860993	29.18408276755769	21.814735571264425
32-33	21.899366272587475	27.072445716424266	29.135718202087247	21.892469808901016
34-35	22.065318610499197	27.018903131037764	29.13662194108616	21.779156317376877
36-37	22.03631712541242	26.947398081094253	29.10668601119264	21.909598782300684
38-39	21.900048594965902	27.103581317840813	29.16044132688556	21.835928760307723
40-41	22.141521445078148	26.956152414901236	28.99917554962137	21.903150590399243
42-43	22.017183810954403	27.087947116138356	28.817525460587017	22.077343612320227
44-45	22.07974137757906	27.168588811847417	28.693513505089562	22.05815630548396
46-47	22.17239398725041	27.17162309594406	28.418971827518895	22.237011089286636
48-49	22.117738226894534	27.269856891586542	28.384675997332337	22.22772888418659
50-51	22.149242403056046	27.436409538973816	28.22706528112829	22.187282776841847
52-53	22.29546200539298	27.377466165719767	28.073388118075503	22.253683710811746
54-55	22.261504206737317	27.302270236608596	28.105842421896334	22.330383134757753
56-57	22.212310155790856	27.36194542692617	28.15578673712531	22.26995768015766
58-59	22.216005140508518	27.329233617668454	28.178320644937436	22.276440596885593
60-61	22.18745594062662	27.220096783632776	28.28138897182557	22.311058303915036
62-63	22.30773703168472	27.35657477385821	28.24374244721929	22.09194574723778
64-65	22.088322714833097	27.265099764872257	28.227851372166153	22.41872614812849
66-67	22.160932892818337	27.339983883151238	28.064078484895933	22.435004739134495
68-69	22.059648204633866	27.53157840049147	28.12110666223484	22.28766673263982
70-71	22.07170779048751	27.303311901286854	28.179721222163597	22.445259086062038
72-73	22.119091209120523	27.367370313787053	27.89076141170124	22.62277706539118
74-75	22.049341568009066	27.45643696585057	27.872844070643225	22.621377395497138
76-77	22.2639231367722	27.1823950050188	27.967813297727133	22.585868560481867
78-79	22.17391794603911	27.185429709208602	27.90170645636748	22.738945888384798
80-81	22.38389253482994	27.29539709537361	27.705771178130146	22.61493919166631
82-83	22.44306665541596	27.47964107990073	27.72460691667748	22.35268534800583
84-85	22.312862910562984	27.292107012609996	27.675355548878034	22.719674527948992
86-87	22.233898477605997	27.537752237159413	27.702340677724035	22.52600860751055
88-89	22.47539160771056	27.313961996031537	27.682786106834946	22.527860289422957
90-91	22.54685909423769	27.53498442228926	27.644862437245234	22.273294046227818
92-93	22.37986533601132	27.481780640965596	27.647649119591843	22.49070490343124
94-95	22.442760889262107	27.33644994278654	27.71744931797999	22.50333984997136
96-97	22.477738668129188	27.55280208214595	27.718415803669473	22.25104344605539
98-99	22.742594937748056	27.998153904809747	27.858443023921968	21.40080813352023
100-101	23.189717335738898	30.546559881386365	25.247198270968813	21.01652451190592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4858.0
1	4281.0
2	4239.5
3	5067.5
4	6165.5
5	8593.5
6	11362.0
7	16232.5
8	21059.0
9	22656.5
10	21419.5
11	19260.0
12	20116.5
13	24979.5
14	35845.5
15	49353.0
16	59138.0
17	63664.5
18	63197.0
19	58584.0
20	52354.0
21	46910.0
22	45040.0
23	48814.5
24	58304.0
25	74578.0
26	95048.5
27	117531.5
28	143765.0
29	172853.5
30	201331.0
31	230826.0
32	262373.5
33	292668.0
34	321370.0
35	348948.0
36	380912.5
37	409885.5
38	430344.5
39	447596.0
40	463799.5
41	479702.5
42	488894.0
43	496952.0
44	507607.0
45	516170.5
46	521421.5
47	523023.5
48	522751.5
49	520696.0
50	514473.5
51	504424.5
52	489326.5
53	468241.5
54	443960.5
55	415356.5
56	382118.0
57	345269.5
58	308013.0
59	269148.5
60	226660.0
61	184534.5
62	145850.0
63	112755.0
64	85006.5
65	63052.0
66	47074.5
67	35715.5
68	26918.0
69	19941.0
70	14743.5
71	10764.0
72	7705.0
73	5179.5
74	3380.5
75	2203.0
76	1421.0
77	860.5
78	529.0
79	317.5
80	198.0
81	129.0
82	95.5
83	75.0
84	58.0
85	39.5
86	23.5
87	15.0
88	12.5
89	10.0
90	6.5
91	3.0
92	1.5
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0032420069791568424
4	0.0
5	0.04850600872642797
6	0.0
7	0.0029783030621919316
8	0.0
9	0.0
10-11	3.8779987788957445E-6
12-13	0.0
14-15	3.8779987788957445E-6
16-17	0.0
18-19	0.0
20-21	5.0415016415129345E-5
22-23	3.8783170275957765E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	7.941839478324876E-4
48-49	2.247704746081699E-4
50-51	7.94904047256016E-4
52-53	0.0013562312167679285
54-55	0.0030893945968074946
56-57	0.0027288273064372155
58-59	0.0064129398725044865
60-61	0.005794123312557303
62-63	0.0065597482017071985
64-65	0.003923394936106995
66-67	0.0064712486128214045
68-69	0.006705330997562426
70-71	0.003106211580045519
72-73	0.002475966558243509
74-75	0.0034506687161109553
76-77	0.0021195164804029506
78-79	0.0
80-81	5.253251588399649E-4
82-83	2.2538928603576733E-4
84-85	0.0
86-87	0.0
88-89	4.838052482612765E-6
90-91	1.467518460037002E-5
92-93	6.77837595158874E-4
94-95	9.008015782844364E-5
96-97	0.0
98-99	1.573734001813571E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	149.0
20-21	643.0
22-23	1308.0
24-25	1676.0
26-27	3860.0
28-29	8450.0
30-31	23102.0
32-33	30619.0
34-35	42448.0
36-37	65644.0
38-39	74177.0
40-41	76952.0
42-43	89676.0
44-45	107423.0
46-47	107306.0
48-49	97693.0
50-51	87564.0
52-53	77499.0
54-55	83892.0
56-57	85451.0
58-59	50958.0
60-61	92730.0
62-63	89333.0
64-65	95583.0
66-67	101873.0
68-69	103474.0
70-71	102188.0
72-73	97011.0
74-75	99240.0
76-77	104532.0
78-79	108229.0
80-81	108270.0
82-83	102816.0
84-85	101761.0
86-87	107184.0
88-89	112086.0
90-91	116741.0
92-93	113321.0
94-95	122304.0
96-97	197015.0
98-99	1128143.0
100-101	8572924.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.8137298905995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.40088741047883	73.20807212100692
2	7.89696752193372	13.079546706325122
3	2.0139046623420436	5.00336870197839
4	0.7630209833504	2.5275445446415863
5	0.3544479979655588	1.4676580381891775
6	0.18968718660020817	0.9425222060890432
7	0.10802546662475804	0.626219427005808
8	0.06529327848501373	0.4325743942503696
9	0.051967092243121134	0.38732298661995324
>10	0.15113019247295892	1.9271333662522334
>50	0.0032088138297979157	0.17475151609761586
>100	0.0014084925445392868	0.1837917583333247
>500	1.6973378630656696E-5	0.007379551164562906
>1k	3.3927750447099654E-5	0.03211468204588036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.755997557791489E-6
2	0.0	7.755997557791489E-6	0.0	0.0	7.755997557791489E-6
3	0.0	7.755997557791489E-6	0.0	0.0	7.755997557791489E-6
4	0.0	3.1023990231165956E-5	0.0	0.0	7.755997557791489E-6
5	0.0	3.1023990231165956E-5	0.0	0.0	7.755997557791489E-6
6	0.0	3.1023990231165956E-5	0.0	0.0	7.755997557791489E-6
7	0.0	3.1023990231165956E-5	0.0	0.0	7.755997557791489E-6
8	0.0	3.1023990231165956E-5	0.0	0.0	7.755997557791489E-6
9	0.0	6.204798046233191E-5	0.0	7.755997557791489E-6	7.755997557791489E-6
10-11	0.0	6.204798046233191E-5	0.0	1.5511995115582978E-5	7.755997557791489E-6
12-13	0.0	6.980397802012339E-5	0.0	1.9389993894478722E-5	7.755997557791489E-6
14-15	0.0	1.0858396580908085E-4	0.0	3.1023990231165956E-5	1.9389993894478722E-5
16-17	0.0	1.0858396580908085E-4	0.0	3.8779987788957444E-5	2.3267992673374465E-5
18-19	0.0	1.1246196458797658E-4	0.0	3.8779987788957444E-5	3.1023990231165956E-5
20-21	0.0	1.1633996336687233E-4	0.0	6.980397802012339E-5	3.1023990231165956E-5
22-23	2.3267992673374465E-5	1.2409596092466382E-4	0.0	8.531597313570638E-5	3.4901989010061697E-5
24-25	2.3267992673374465E-5	1.4736395359803828E-4	0.0	1.047059670301851E-4	4.265798656785319E-5
26-27	2.714599145227021E-5	1.5511995115582977E-4	0.0	1.7838794382920426E-4	4.653598534674893E-5
28-29	3.1023990231165956E-5	1.5511995115582977E-4	0.0	5.00261842477551E-4	4.653598534674893E-5
30-31	3.4901989010061697E-5	1.6287594871362127E-4	0.0	0.0016326374859151086	4.653598534674893E-5
32-33	3.8779987788957444E-5	1.6287594871362127E-4	0.0	0.00416497068853403	6.204798046233191E-5
34-35	3.8779987788957444E-5	1.8614394138699575E-4	0.0	0.007515561633499953	8.531597313570638E-5
36-37	5.041398412564468E-5	2.094119340603702E-4	0.0	0.013146415860456574	9.307197069349786E-5
38-39	5.4291982904540425E-5	2.171679316181617E-4	0.0	0.023651914552485144	1.0082796825128936E-4
40-41	5.4291982904540425E-5	2.4819192184932765E-4	0.0	0.05675839012791811	1.0082796825128936E-4
42-43	5.4291982904540425E-5	2.7921591208049357E-4	0.0	0.06666279900921784	1.0082796825128936E-4
44-45	5.4291982904540425E-5	3.2575189742724254E-4	0.0	0.08112773445449897	1.047059670301851E-4
46-47	5.4291982904540425E-5	3.567758876584085E-4	0.0	0.09723306338325301	1.1633996336687233E-4
48-49	5.4291982904540425E-5	3.567758876584085E-4	0.0	0.11182985078701659	1.396079560402468E-4
50-51	5.4291982904540425E-5	3.839218791106787E-4	0.0	0.12642663819078015	1.396079560402468E-4
52-53	5.8169981683436165E-5	3.8779987788957444E-4	0.0	0.15204857612294437	1.5124195237693403E-4
54-55	6.204798046233191E-5	3.8779987788957444E-4	0.0	0.2152522002213872	1.6287594871362127E-4
56-57	6.204798046233191E-5	4.033118730051574E-4	0.0	0.28556031808276705	1.7063194627141276E-4
58-59	6.204798046233191E-5	4.033118730051574E-4	0.0	0.35485240026407616	1.7838794382920426E-4
60-61	6.204798046233191E-5	4.0718987178405317E-4	0.0	0.7016657090595015	1.8614394138699572E-4
62-63	6.98039780201234E-5	4.110678705629489E-4	0.0	0.7085879368798305	1.8614394138699572E-4
64-65	6.98039780201234E-5	5.041398412564468E-4	0.0	0.7157389666281142	1.8614394138699572E-4
66-67	6.98039780201234E-5	5.041398412564468E-4	0.0	0.7215986227830257	1.8614394138699572E-4
68-69	6.98039780201234E-5	5.196518363720298E-4	0.0	0.7276832028671132	1.9002194016589147E-4
70-71	6.98039780201234E-5	5.274078339298213E-4	0.0	0.7334303970574365	1.9389993894478722E-4
72-73	6.98039780201234E-5	5.274078339298213E-4	0.0	0.7377504876971264	2.4043592429153616E-4
74-75	6.98039780201234E-5	5.31285832708717E-4	0.0	0.7414035625468463	2.4043592429153616E-4
76-77	6.98039780201234E-5	5.62309822939883E-4	0.0	0.744645569526003	2.4819192184932765E-4
78-79	6.98039780201234E-5	5.661878217187787E-4	0.0	0.7473718026675669	2.520699206282234E-4
80-81	6.98039780201234E-5	5.661878217187787E-4	0.0	0.7485662262914667	2.6370391696491064E-4
82-83	6.98039780201234E-5	5.700658204976744E-4	0.0	0.7489966841559241	2.753379133015979E-4
84-85	6.98039780201234E-5	5.816998168343616E-4	0.0	0.7494620440093915	2.792159120804936E-4
86-87	6.98039780201234E-5	5.894558143921531E-4	0.0	0.7496481879507786	2.830939108593893E-4
88-89	6.98039780201234E-5	6.127238070655276E-4	0.0	0.7497412599214721	2.8697190963828507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19540	0.0	52.679005	1
GTATCAA	35055	0.0	36.655304	1
TCAACGC	43695	0.0	29.317606	4
ATCAACG	43915	0.0	29.181051	3
CAACGCA	44780	0.0	28.626602	5
TATCAAC	45170	0.0	28.533337	2
AACGCAG	46280	0.0	27.731482	6
GTGGTAT	8835	0.0	26.77329	1
TGGTATC	8820	0.0	25.4435	2
ACGCAGA	52610	0.0	24.394848	7
CGCAGAG	54060	0.0	23.795488	8
GAGACCG	11160	0.0	22.391262	88-89
CGAGACC	10910	0.0	22.340078	88-89
AGACCGA	11015	0.0	22.159828	90-91
GACCGAG	11470	0.0	21.972836	90-91
ACCGAGG	11870	0.0	21.216486	92-93
GCAGAGT	60700	0.0	21.199623	9
ATCTCCG	11145	0.0	20.957216	74-75
ACGAGAC	12020	0.0	20.736498	86-87
GAGTACT	37360	0.0	20.304638	12-13
>>END_MODULE