FastQCFastQC Report
Fri 27 May 2016
SRR2139498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14379321
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA254000.040.2618181
TATCTCG144650.034.0796294-95
GTATCAA417650.032.065261
CCGTCTT74850.029.95893992-93
TATGCCG76050.028.28551388-89
TGCCGTC79050.027.87998290-91
TCAACGC501750.026.4795674
GCCGTCT79100.026.45871590-91
ATCAACG505250.026.2795543
TCTCGTA73750.026.19020882-83
CAACGCA512600.025.8933535
TATCAAC519400.025.775242
AACGCAG528050.025.2167196
ATGCCGT81600.025.0519988-89
CTCGTAT87300.024.38765784-85
CGTATGC95250.023.01251486-87
CTATCTC232400.022.75369594-95
ACGCAGA592200.022.4034947
TCGTATG90800.022.3563384-85
CGCAGAG599900.022.2032998
Produced by FastQC (version 0.11.5)