##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14379321
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.199082557514362	31.0	25.0	33.0	18.0	33.0
2	30.969822079916014	33.0	31.0	33.0	27.0	33.0
3	31.358129984023584	33.0	31.0	33.0	28.0	34.0
4	31.84822753452684	33.0	32.0	33.0	29.0	34.0
5	32.19684684694082	33.0	33.0	34.0	31.0	34.0
6	35.54357907442222	38.0	36.0	38.0	29.0	38.0
7	35.818823851279205	38.0	36.0	38.0	31.0	38.0
8	36.083776139360126	38.0	37.0	38.0	33.0	38.0
9	36.561821938601966	38.0	37.0	38.0	34.0	38.0
10-11	36.653453316745626	38.0	38.0	38.0	34.0	38.0
12-13	36.72550379812788	38.0	38.0	38.0	34.0	38.0
14-15	36.715953451487735	38.0	38.0	38.0	34.0	38.0
16-17	36.73180541695953	38.0	38.0	38.0	34.0	38.0
18-19	36.76462198736644	38.0	38.0	38.0	34.5	38.0
20-21	36.78709402884049	38.0	38.0	38.0	35.0	38.0
22-23	36.81210757818042	38.0	38.0	38.0	35.0	38.0
24-25	36.80854314913465	38.0	38.0	38.0	35.0	38.0
26-27	36.77464974453745	38.0	38.0	38.0	35.0	38.0
28-29	36.76927669200535	38.0	38.0	38.0	35.0	38.0
30-31	36.749993968186914	38.0	38.0	38.0	35.0	38.0
32-33	36.733078765700256	38.0	38.0	38.0	34.5	38.0
34-35	36.7068018948532	38.0	38.0	38.0	34.0	38.0
36-37	36.68350861881352	38.0	38.0	38.0	34.0	38.0
38-39	36.674119522991724	38.0	38.0	38.0	34.0	38.0
40-41	36.65822684923767	38.0	38.0	38.0	34.0	38.0
42-43	36.621353712860554	38.0	38.0	38.0	34.0	38.0
44-45	36.5744433939633	38.0	38.0	38.0	34.0	38.0
46-47	36.531292045768666	38.0	38.0	38.0	34.0	38.0
48-49	36.49024254288203	38.0	38.0	38.0	34.0	38.0
50-51	36.468878678366146	38.0	38.0	38.0	34.0	38.0
52-53	36.43252264738135	38.0	38.0	38.0	34.0	38.0
54-55	36.365371466964916	38.0	38.0	38.0	34.0	38.0
56-57	36.37372882295122	38.0	38.0	38.0	34.0	38.0
58-59	36.37119672908348	38.0	38.0	38.0	34.0	38.0
60-61	36.33047016365239	38.0	38.0	38.0	33.5	38.0
62-63	36.33347811956418	38.0	38.0	38.0	33.0	38.0
64-65	36.35872744943207	38.0	38.0	38.0	34.0	38.0
66-67	36.34004155362855	38.0	38.0	38.0	33.5	38.0
68-69	36.324880112073885	38.0	38.0	38.0	33.0	38.0
70-71	36.32454950784797	38.0	38.0	38.0	33.0	38.0
72-73	36.31300266813308	38.0	38.0	38.0	33.0	38.0
74-75	36.29430150749211	38.0	37.0	38.0	33.0	38.0
76-77	36.25360312260375	38.0	37.0	38.0	33.0	38.0
78-79	36.24207730238338	38.0	37.0	38.0	33.0	38.0
80-81	36.226818895019264	38.0	37.0	38.0	33.0	38.0
82-83	36.183172846574806	38.0	37.0	38.0	33.0	38.0
84-85	36.14340349205729	38.0	37.0	38.0	33.0	38.0
86-87	36.166650235409804	38.0	37.0	38.0	33.0	38.0
88-89	36.16297703827648	38.0	37.0	38.0	33.0	38.0
90-91	36.15867335370713	38.0	37.0	38.0	33.0	38.0
92-93	36.15108913088386	38.0	37.0	38.0	33.0	38.0
94-95	36.13022321076423	38.0	37.0	38.0	33.0	38.0
96-97	36.071209223620635	38.0	37.0	38.0	33.0	38.0
98-99	36.09725437179052	38.0	37.0	38.0	33.0	38.0
100-101	35.60420834843288	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	15.0
19	47.0
20	141.0
21	763.0
22	3263.0
23	9982.0
24	22498.0
25	41653.0
26	68464.0
27	103099.0
28	145418.0
29	194221.0
30	250395.0
31	318198.0
32	403682.0
33	522307.0
34	719095.0
35	1109702.0
36	2331177.0
37	8135196.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.61321810675205	19.971158582522776	15.714045190311838	23.701578120413338
2	14.534413690326545	21.545377559900082	41.13073211175966	22.789476638013713
3	18.52189591925556	25.686166534921767	30.781787184158233	25.010150361664447
4	12.86636552588262	18.097558292217	37.848810802679765	31.18726537922062
5	13.829292409418844	37.07006744355253	35.13080525713346	13.96983488989517
6	30.33641852768987	37.670339232290594	18.255208295301287	13.738033944718254
7	26.73020423335729	32.50867330208615	22.66073992472996	18.1003825398266
8	24.324382215265935	37.0338557710757	20.574740629268934	18.06702138438943
9	25.19837341415495	17.263708070777472	21.335374598007792	36.202543917059785
10-11	24.46977240683939	26.82288081693429	28.300791403877533	20.406555372348784
12-13	25.177308441754654	24.324702119105623	28.695819503577397	21.802169935562326
14-15	22.2457204769475	25.713343090633956	26.54174465917839	25.49919177324016
16-17	21.673961517376238	28.806763546067298	27.268512887360952	22.250762049195508
18-19	21.694678768211656	27.90779898438876	29.127335011159428	21.270187236240155
20-21	22.508336337152514	26.959241784343007	29.11700860363823	21.41541327486625
22-23	22.29774956899034	26.78404753750695	29.02046742952432	21.89773546397839
24-25	22.07622065195643	26.946780959955536	29.05251594357157	21.924482444516467
26-27	21.942248918702123	27.054681635433038	29.1050325137245	21.898036932140343
28-29	21.873653841803684	27.09631784733013	29.124273740149054	21.905754570717136
30-31	22.08940092403476	26.96912292635763	29.039597140518087	21.901879009089516
32-33	21.91945643933841	27.139671523726154	28.957121337136332	21.983750699799103
34-35	22.14146670685915	27.089897593861327	28.959778800444823	21.8088568988347
36-37	22.06547695852235	27.0054821106588	28.987076835652363	21.94196409516649
38-39	21.93865847730383	27.18109357551776	29.006339192469127	21.873908754709287
40-41	22.111582832036316	26.98281459784472	28.91862329638173	21.986979273737234
42-43	22.09004100981549	27.069038639504427	28.737674295103567	22.103246055576516
44-45	22.02011240871676	27.1256169708316	28.627755448693915	22.226515171757725
46-47	22.161415160099974	27.12365691454118	28.41720739604534	22.297720529313512
48-49	22.0528978134261	27.32512458000859	28.433632188129	22.188345418436313
50-51	21.999667727661237	27.46992995994681	28.320239065499305	22.21016324689265
52-53	22.16081700889177	27.430028841947152	27.944358790540765	22.464795358620318
54-55	22.140821853325782	27.158202833902003	28.128363850489468	22.57261146228275
56-57	22.122070932814562	26.947989843427212	28.38287681612203	22.547062407636194
58-59	21.99687227307928	26.758665067724834	28.54846188248518	22.696000776710708
60-61	22.078037470500874	26.622174954752698	28.78758012804156	22.512207446704867
62-63	21.857747286847562	27.123234677618314	28.75784540985706	22.26117262567706
64-65	21.525328469435458	27.250289208301925	28.573889472139758	22.65049285012286
66-67	21.60350898245433	27.736508090816432	28.114246611548154	22.545736315181085
68-69	21.645129680557844	27.94119511362333	27.8252919840052	22.588383221813626
70-71	21.67422252241817	27.58086068987388	27.81564999845068	22.92926678925727
72-73	21.618352179365722	27.297100819025406	27.772697771114927	23.311849230493944
74-75	21.88734667526169	27.154551619104282	27.757959382511583	23.200142323122446
76-77	22.27888076931445	26.96479718032807	27.67677750058169	23.07954454977579
78-79	22.42474445070151	26.84558270984219	27.121042576976002	23.608630262480297
80-81	22.342869385353296	27.250734099677278	27.033957785269664	23.372438729699766
82-83	22.34872193975049	27.34323009220048	27.13127671109562	23.176771256953412
84-85	22.365124018888974	27.386223973134598	27.026199545552114	23.222452462424314
86-87	22.59551855703786	27.65018113211092	26.98228180034275	22.77201851050847
88-89	22.74515416328205	27.4320956069404	27.184528217875425	22.638222011902126
90-91	22.522888000445356	27.47315320966634	27.38510089695232	22.618857892935992
92-93	22.405854539094133	27.043256375649925	27.303246558822856	23.24764252643308
94-95	22.208228193740403	27.060488736202572	27.84982309130099	22.881459978756034
96-97	21.94648184620645	27.31220666303805	27.999882220245066	22.741429270510434
98-99	22.2589336886554	27.506832592624885	28.279776161631574	21.95445755708814
100-101	23.02469218277368	30.249701516230488	25.594916047008574	21.130690253987254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4766.0
1	4215.5
2	4334.5
3	5294.5
4	6412.0
5	8843.5
6	11610.5
7	16422.5
8	21296.5
9	22800.0
10	21485.5
11	19555.5
12	20508.5
13	25109.0
14	35604.5
15	48747.5
16	57850.0
17	61753.5
18	61173.0
19	56874.5
20	50904.5
21	45879.0
22	44389.5
23	48756.0
24	58643.0
25	74703.0
26	95744.0
27	119249.0
28	147560.0
29	180215.5
30	212605.0
31	245413.5
32	280062.5
33	315159.0
34	347886.5
35	377717.0
36	412774.0
37	444871.0
38	468165.0
39	488453.0
40	509392.5
41	530118.0
42	542604.5
43	555078.5
44	569156.5
45	581084.5
46	591530.5
47	598246.5
48	599485.5
49	594281.0
50	584103.0
51	567501.0
52	544310.5
53	516339.5
54	487149.5
55	455007.0
56	416337.0
57	374728.5
58	335180.0
59	291537.0
60	242228.5
61	195484.5
62	153557.0
63	117225.5
64	87106.0
65	64488.5
66	48464.5
67	36876.0
68	27767.5
69	20499.5
70	14777.5
71	10232.5
72	6970.0
73	4561.0
74	2905.5
75	1800.0
76	1129.0
77	678.0
78	404.5
79	252.5
80	156.5
81	99.5
82	74.5
83	56.0
84	40.5
85	29.0
86	21.5
87	12.5
88	4.5
89	3.5
90	6.0
91	6.0
92	5.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0036371675686216335
4	0.0
5	0.049752001502713514
6	0.0
7	0.0029625877327587305
8	0.0
9	0.0
10-11	3.4772156487778527E-6
12-13	0.0
14-15	1.7386078243889265E-5
16-17	0.0
18-19	0.0
20-21	5.5636982597795034E-5
22-23	3.4775669286445343E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.591424468482647E-6
46-47	7.20516735775325E-4
48-49	1.8612925384723695E-4
50-51	7.021860042932608E-4
52-53	0.0012897811086241455
54-55	0.003150866832311533
56-57	0.0027901487323196065
58-59	0.006398122743393587
60-61	0.005641096725695919
62-63	0.006558054711973528
64-65	0.0038233175284041417
66-67	0.0063906757951692345
68-69	0.006759473795071138
70-71	0.0034655615710715783
72-73	0.0026068288394760393
74-75	0.0035909524812872675
76-77	0.0021043896140644563
78-79	0.0
80-81	5.574182390640802E-4
82-83	2.3247156261022007E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.2729226316351569E-5
92-93	7.164576412223815E-4
94-95	1.0845830296681613E-4
96-97	4.394766782032224E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	202.0
20-21	949.0
22-23	1416.0
24-25	2017.0
26-27	5561.0
28-29	9166.0
30-31	26606.0
32-33	34789.0
34-35	48125.0
36-37	73318.0
38-39	50280.0
40-41	83853.0
42-43	93893.0
44-45	111084.0
46-47	111614.0
48-49	102539.0
50-51	91943.0
52-53	9721.0
54-55	8387.0
56-57	7924.0
58-59	8013.0
60-61	100676.0
62-63	98015.0
64-65	104062.0
66-67	110729.0
68-69	114228.0
70-71	112059.0
72-73	105450.0
74-75	108160.0
76-77	114616.0
78-79	120834.0
80-81	120696.0
82-83	114616.0
84-85	114535.0
86-87	118897.0
88-89	124228.0
90-91	129795.0
92-93	127392.0
94-95	138998.0
96-97	224005.0
98-99	1361456.0
100-101	9834474.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.96662464220978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.24696837046847	70.6409253922247
2	8.520169006177174	13.796986516226736
3	2.2068313006661713	5.360390447091524
4	0.904350131812227	2.928887106702941
5	0.4069390449464347	1.6474240452218656
6	0.23011732806315438	1.1179093994974603
7	0.13534525442809398	0.7670913888668707
8	0.09322020290670421	0.6038180142254203
9	0.060009881146951535	0.4372917769483962
>10	0.1913852406212548	2.3376072547106164
>50	0.0037370454335174112	0.20481533636608876
>100	8.616966280943717E-4	0.10946389182051833
>500	3.2759776349813174E-5	0.014349721709334151
>1k	3.273692543834004E-5	0.0330397083875834
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.9544312975557055E-6	0.0	0.0	0.0
3	0.0	6.9544312975557055E-6	0.0	0.0	0.0
4	0.0	4.172658778533423E-5	0.0	6.9544312975557055E-6	0.0
5	1.3908862595111411E-5	4.172658778533423E-5	0.0	2.0863293892667116E-5	0.0
6	1.3908862595111411E-5	4.172658778533423E-5	0.0	2.0863293892667116E-5	6.9544312975557055E-6
7	1.3908862595111411E-5	4.172658778533423E-5	0.0	2.0863293892667116E-5	6.9544312975557055E-6
8	1.3908862595111411E-5	4.8681019082889935E-5	0.0	2.0863293892667116E-5	6.9544312975557055E-6
9	2.0863293892667116E-5	9.040760686822416E-5	0.0	2.7817725190222822E-5	6.9544312975557055E-6
10-11	2.7817725190222822E-5	9.040760686822416E-5	0.0	2.7817725190222822E-5	6.9544312975557055E-6
12-13	4.172658778533423E-5	9.040760686822416E-5	0.0	3.4772156487778524E-5	6.9544312975557055E-6
14-15	5.215823473166779E-5	1.2170254770722485E-4	0.0	3.824937213655638E-5	6.9544312975557055E-6
16-17	6.606709732677921E-5	1.2865697900478056E-4	0.0	4.172658778533423E-5	6.9544312975557055E-6
18-19	7.99759599218906E-5	1.5647470419500337E-4	0.0	4.8681019082889935E-5	6.9544312975557055E-6
20-21	9.736203816577987E-5	1.7386078243889264E-4	0.0	6.954431297555706E-5	6.9544312975557055E-6
22-23	1.0431646946333558E-4	1.8776964503400403E-4	0.0	1.0779368511211343E-4	6.9544312975557055E-6
24-25	1.18225332058447E-4	1.9472407633155974E-4	0.0	1.5299748854622551E-4	6.9544312975557055E-6
26-27	1.321341946535584E-4	2.0167850762911545E-4	0.0	2.6774560495589463E-4	6.9544312975557055E-6
28-29	1.321341946535584E-4	2.0167850762911545E-4	0.0	8.72781127843241E-4	6.9544312975557055E-6
30-31	1.390886259511141E-4	2.0863293892667116E-4	0.0	0.0026113889522321673	6.9544312975557055E-6
32-33	1.460430572486698E-4	2.0863293892667116E-4	0.0	0.006923136356716705	6.9544312975557055E-6
34-35	1.6342913549255905E-4	2.468823110632275E-4	0.0	0.012643156098956273	6.9544312975557055E-6
36-37	1.982012919803376E-4	2.781772519022282E-4	0.0	0.021207538241896123	6.9544312975557055E-6
38-39	2.0863293892667116E-4	2.990405457948953E-4	0.0	0.046782459338657226	6.9544312975557055E-6
40-41	2.1558737022422687E-4	3.2685827098511815E-4	0.0	0.3190553990692606	6.9544312975557055E-6
42-43	2.1558737022422687E-4	3.30335486633896E-4	0.0	0.34363583649047125	6.9544312975557055E-6
44-45	2.1558737022422687E-4	3.5467599617534094E-4	0.0	0.3855049901174054	6.9544312975557055E-6
46-47	2.1558737022422687E-4	3.7553929006800806E-4	0.0	0.4338278559884712	6.9544312975557055E-6
48-49	2.2949623281933828E-4	3.7553929006800806E-4	0.0	0.47873609609243717	6.9544312975557055E-6
50-51	2.4340509541444968E-4	4.0683423090700875E-4	0.0	0.5230845044769499	6.9544312975557055E-6
52-53	2.4340509541444968E-4	4.103114465557866E-4	0.0	0.6957247842231216	6.9544312975557055E-6
54-55	2.4688231106322756E-4	4.2074309350212017E-4	0.0	1.2947760189789213	6.9544312975557055E-6
56-57	2.503595267120054E-4	4.3117474044845373E-4	0.0	1.951691599346033	6.9544312975557055E-6
58-59	2.503595267120054E-4	4.4508360304356515E-4	0.0	2.625496016119259	6.9544312975557055E-6
60-61	2.5731395800956107E-4	4.5551524998989866E-4	0.0	3.1343274136518686	6.9544312975557055E-6
62-63	2.5731395800956107E-4	4.7290132823378793E-4	0.0	3.143444673082964	6.9544312975557055E-6
64-65	2.5731395800956107E-4	5.389684255605672E-4	0.0	3.149428961214511	6.9544312975557055E-6
66-67	2.607911736583389E-4	5.42445641209345E-4	0.0	3.1548221226857653	6.9544312975557055E-6
68-69	2.712228206046725E-4	5.459228568581228E-4	0.0	3.160086627178015	6.9544312975557055E-6
70-71	2.712228206046725E-4	5.528772881556786E-4	0.0	3.164944297439358	6.9544312975557055E-6
72-73	2.781772519022282E-4	5.563545038044564E-4	0.0	3.1688248701033936	6.9544312975557055E-6
74-75	2.781772519022282E-4	5.702633663995678E-4	0.0	3.1718813426586694	6.9544312975557055E-6
76-77	2.781772519022282E-4	6.050355228873463E-4	0.0	3.174670069608989	6.9544312975557055E-6
78-79	2.851316831997839E-4	6.189443854824578E-4	0.0	3.1772745041299237	6.9544312975557055E-6
80-81	2.920861144973396E-4	6.398076793751249E-4	0.0	3.1784219852940208	6.9544312975557055E-6
82-83	2.9556333014611746E-4	6.467621106726806E-4	0.0	3.178860114465767	6.9544312975557055E-6
84-85	3.0599497709245103E-4	6.467621106726806E-4	0.0	3.179287811990566	6.9544312975557055E-6
86-87	3.0599497709245103E-4	6.467621106726806E-4	0.0	3.17941994618522	6.9544312975557055E-6
88-89	3.0599497709245103E-4	6.676254045653477E-4	0.0	3.1794686272043027	6.9544312975557055E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25400	0.0	40.261818	1
TATCTCG	14465	0.0	34.07962	94-95
GTATCAA	41765	0.0	32.06526	1
CCGTCTT	7485	0.0	29.958939	92-93
TATGCCG	7605	0.0	28.285513	88-89
TGCCGTC	7905	0.0	27.879982	90-91
TCAACGC	50175	0.0	26.479567	4
GCCGTCT	7910	0.0	26.458715	90-91
ATCAACG	50525	0.0	26.279554	3
TCTCGTA	7375	0.0	26.190208	82-83
CAACGCA	51260	0.0	25.893353	5
TATCAAC	51940	0.0	25.77524	2
AACGCAG	52805	0.0	25.216719	6
ATGCCGT	8160	0.0	25.05199	88-89
CTCGTAT	8730	0.0	24.387657	84-85
CGTATGC	9525	0.0	23.012514	86-87
CTATCTC	23240	0.0	22.753695	94-95
ACGCAGA	59220	0.0	22.403494	7
TCGTATG	9080	0.0	22.35633	84-85
CGCAGAG	59990	0.0	22.203299	8
>>END_MODULE