FastQCFastQC Report
Fri 27 May 2016
SRR2139497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10353966
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144300.052.0832981
GTATCAA273400.038.8644181
TCAACGC329600.031.7134694
ATCAACG331650.031.3741783
CAACGCA339400.030.8359385
TATCAAC341750.030.7250082
AACGCAG349250.030.025386
GTGGTAT70400.026.4435731
ACGCAGA399000.026.2707947
TGGTATC66950.025.99982
CGCAGAG412550.025.4281338
GCAGAGT468850.022.3378669
GAGTACT279550.020.72420312-13
CAGAGTA460500.019.489910-11
GTACATG324650.019.3847271
AGAGTAC428200.018.98403210-11
GTACTTT306150.018.8251814-15
TACATGG329650.018.6190092
ACATGGG331100.017.9765193
AGTACTT298700.017.40107312-13
Produced by FastQC (version 0.11.5)