##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10353966
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.51004948248816	33.0	31.0	33.0	18.0	33.0
2	31.53138014940362	33.0	31.0	33.0	28.0	34.0
3	31.14735918584241	33.0	31.0	33.0	27.0	34.0
4	31.777301953666836	33.0	32.0	33.0	29.0	34.0
5	31.862356028598125	33.0	32.0	33.0	30.0	34.0
6	34.928738610885915	37.0	34.0	38.0	29.0	38.0
7	35.94816836369754	38.0	36.0	38.0	31.0	38.0
8	36.0646803360181	38.0	37.0	38.0	32.0	38.0
9	36.49555069043109	38.0	37.0	38.0	34.0	38.0
10-11	36.651773919288516	38.0	38.0	38.0	34.0	38.0
12-13	36.72063144692575	38.0	38.0	38.0	34.0	38.0
14-15	36.75932222493294	38.0	38.0	38.0	34.0	38.0
16-17	36.7724899811338	38.0	38.0	38.0	34.5	38.0
18-19	36.79849446096307	38.0	38.0	38.0	35.0	38.0
20-21	36.81575738090808	38.0	38.0	38.0	35.0	38.0
22-23	36.833282555524235	38.0	38.0	38.0	35.0	38.0
24-25	36.83354252680992	38.0	38.0	38.0	35.0	38.0
26-27	36.79586137006387	38.0	38.0	38.0	35.0	38.0
28-29	36.79218086233905	38.0	38.0	38.0	35.0	38.0
30-31	36.77149853893019	38.0	38.0	38.0	35.0	38.0
32-33	36.75243951462063	38.0	38.0	38.0	35.0	38.0
34-35	36.729559781714855	38.0	38.0	38.0	34.5	38.0
36-37	36.7062890778037	38.0	38.0	38.0	34.0	38.0
38-39	36.69105613209733	38.0	38.0	38.0	34.0	38.0
40-41	36.6808244939207	38.0	38.0	38.0	34.0	38.0
42-43	36.63572591786138	38.0	38.0	38.0	34.0	38.0
44-45	36.59071816480227	38.0	38.0	38.0	34.0	38.0
46-47	36.54976413408893	38.0	38.0	38.0	34.0	38.0
48-49	36.507245369166114	38.0	38.0	38.0	34.0	38.0
50-51	36.490455998994136	38.0	38.0	38.0	34.0	38.0
52-53	36.460583233456745	38.0	38.0	38.0	34.0	38.0
54-55	36.389361693931235	38.0	38.0	38.0	34.0	38.0
56-57	36.39946814216057	38.0	38.0	38.0	34.0	38.0
58-59	36.39667188299037	38.0	38.0	38.0	34.0	38.0
60-61	36.357408432433814	38.0	38.0	38.0	33.5	38.0
62-63	36.36128561663749	38.0	38.0	38.0	33.5	38.0
64-65	36.3807491418439	38.0	38.0	38.0	34.0	38.0
66-67	36.36233087898269	38.0	38.0	38.0	34.0	38.0
68-69	36.34810372377356	38.0	38.0	38.0	34.0	38.0
70-71	36.3468848525081	38.0	38.0	38.0	34.0	38.0
72-73	36.336641041538414	38.0	38.0	38.0	33.0	38.0
74-75	36.325058520138015	38.0	38.0	38.0	33.0	38.0
76-77	36.28881199403452	38.0	37.5	38.0	33.0	38.0
78-79	36.28467903524216	38.0	37.0	38.0	33.0	38.0
80-81	36.27445646853584	38.0	37.0	38.0	33.0	38.0
82-83	36.247788750366155	38.0	37.0	38.0	33.0	38.0
84-85	36.202773220814436	38.0	37.0	38.0	33.0	38.0
86-87	36.215041440307154	38.0	37.0	38.0	33.0	38.0
88-89	36.21501228144096	38.0	37.0	38.0	33.0	38.0
90-91	36.206784870221426	38.0	37.0	38.0	33.0	38.0
92-93	36.19471589844971	38.0	37.0	38.0	33.0	38.0
94-95	36.17621097566217	38.0	37.0	38.0	33.0	38.0
96-97	36.168190947921815	38.0	37.0	38.0	33.0	38.0
98-99	36.21103949486279	38.0	37.0	38.0	33.0	38.0
100-101	35.684291823727584	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	4.0
19	23.0
20	70.0
21	459.0
22	2146.0
23	6739.0
24	15272.0
25	29075.0
26	48016.0
27	72310.0
28	101977.0
29	136321.0
30	175545.0
31	222774.0
32	284333.0
33	368823.0
34	508120.0
35	782514.0
36	1613547.0
37	5985895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56126898620297	20.08389828593217	14.468880813400393	24.885951914464467
2	14.166687431656625	20.646281820898388	42.62156163155259	22.5654691158924
3	18.247737237853435	25.601860298541368	29.900198066239135	26.250204397366055
4	12.531188532007928	18.140555995644565	38.88227950526397	30.445975967083534
5	13.82084163607685	36.82900698430602	35.211717423039715	14.13843395657742
6	29.868583690539452	37.85166959211572	18.576215143066918	13.703531574277914
7	26.548216996283053	32.86361179610827	22.740043802901624	17.848127404707054
8	24.13017388699171	36.98475540676877	20.811213789962224	18.073856916277297
9	25.283191001399853	17.46325031393767	21.419396200451114	35.83416248421136
10-11	24.16575445582881	26.9569409441754	28.487639422420358	20.389665177575434
12-13	25.02345960958342	24.45951145676932	28.80357150100744	21.71345743263982
14-15	22.192668325258406	25.785741297451814	26.64003242429506	25.381557952994722
16-17	21.549462302657744	28.994097527459527	27.310988852001252	22.145451317881477
18-19	21.602248838754154	27.968558135114602	29.303428270867414	21.12576475526383
20-21	22.43801455535835	27.0139272303554	29.287845218676868	21.260212995609383
22-23	22.207329257531246	26.893155851521705	29.144657232359357	21.7548576585877
24-25	21.9422524284712	27.06816952597683	29.21426776759744	21.775310277954528
26-27	21.838061961066842	27.16134468500916	29.223277347381888	21.77731600654211
28-29	21.783758913548922	27.157903862577736	29.27429802713982	21.78403919673352
30-31	22.027289995759414	27.076447578683645	29.145773901867088	21.75048852368985
32-33	21.826214867919937	27.266552511194224	29.07018603607044	21.837046584815397
34-35	22.00087516634113	27.23353893992136	29.081291582245832	21.684294311491673
36-37	21.955870242038817	27.107760011403332	29.05181422522573	21.88455552133212
38-39	21.808329963130866	27.25768734842674	29.143617327098802	21.790365361343593
40-41	22.019868546495278	27.14052650295993	29.012496778608398	21.82710817193639
42-43	21.94934577629001	27.222912853776222	28.823485004631344	22.004256365302428
44-45	21.982966707655873	27.292900659480797	28.7373472726663	21.98678536019703
46-47	22.086186278364693	27.310602076534813	28.459371054980494	22.143840590120007
48-49	22.009759791805234	27.426238461285347	28.41820652874286	22.145795218166555
50-51	22.01088025263003	27.55257058470704	28.290197164915504	22.146351997747427
52-53	22.204400458158684	27.46044120010765	28.166726070631487	22.16843227110218
54-55	22.191363037544967	27.430067061489495	28.16111295942208	22.21745694154346
56-57	22.129710593818867	27.48453042896713	28.206271508538315	22.179487468675692
58-59	22.05932287603257	27.511295618547436	28.24309524262587	22.186286262794123
60-61	22.156212962381222	27.520180731189907	28.209038808297056	22.114567498131816
62-63	22.11316203092519	27.608308894786106	28.181459476235354	22.097069598053352
64-65	22.083313028138125	27.5275040637464	28.160999080580762	22.22818382753471
66-67	22.139152034013254	27.633709634730625	27.99629407174391	22.230844259512214
68-69	22.062325895308906	27.582704553023767	28.07983616789923	22.275133383768093
70-71	22.082976146208843	27.384627631031083	28.124550500878403	22.40784572188167
72-73	22.13427909109404	27.394565956889505	28.021877352902326	22.44927759911413
74-75	22.043623386494076	27.53599437857801	28.014213182316194	22.40616905261172
76-77	22.227131184992718	27.462265352437782	27.92964094656245	22.380962516007052
78-79	22.164550321445986	27.464432231699742	27.84336874677466	22.527648700079606
80-81	22.20329733264725	27.567794208668538	27.804280407199016	22.424628051485197
82-83	22.260282072125857	27.50983815140246	27.892877728659354	22.337002047812327
84-85	22.31870587703416	27.53734099709377	27.809766368558957	22.334186757313105
86-87	22.248149095230215	27.58070426136791	27.863209037168513	22.30793760623336
88-89	22.309058420146727	27.58414461024199	27.842993100231656	22.26380386937963
90-91	22.33772160622323	27.5989820941692	27.841345310152484	22.221950989455085
92-93	22.396444835404967	27.549328878970584	27.847377541200224	22.20684874442422
94-95	22.259992452105184	27.634511280101698	27.85460054506143	22.25089572273169
96-97	22.307290200292584	27.621182880529233	27.85935735470933	22.21216956446885
98-99	22.505269174318986	28.202594120821534	27.930205186202706	21.361931518656775
100-101	23.097214023881175	30.69637782119976	25.327148939053	20.87925921586606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2479.0
1	2331.5
2	2841.0
3	3732.5
4	4680.5
5	6614.0
6	8553.5
7	11264.5
8	14477.0
9	15987.5
10	15289.0
11	14191.5
12	15076.5
13	18580.0
14	26266.5
15	35618.5
16	42661.5
17	46793.5
18	46966.0
19	43632.0
20	39171.5
21	35295.0
22	34360.0
23	38299.0
24	46321.5
25	59116.0
26	75926.0
27	95234.0
28	117630.0
29	141463.0
30	165132.5
31	189493.5
32	215886.5
33	243539.5
34	269766.0
35	294029.5
36	320712.5
37	342729.0
38	358301.0
39	373054.5
40	387290.5
41	400319.5
42	407646.5
43	414697.0
44	423853.5
45	429943.0
46	430869.0
47	427639.5
48	423028.0
49	419092.5
50	414599.5
51	404838.5
52	388234.0
53	367316.0
54	344945.5
55	321536.0
56	294524.0
57	265446.0
58	237662.0
59	207244.0
60	173788.0
61	142024.5
62	112693.0
63	86701.0
64	64999.5
65	48119.5
66	35708.0
67	26920.5
68	20291.0
69	14720.5
70	10489.5
71	7282.0
72	4830.0
73	3120.0
74	1993.5
75	1201.5
76	735.5
77	455.5
78	292.0
79	196.5
80	138.0
81	87.5
82	49.0
83	30.0
84	23.5
85	22.0
86	16.0
87	12.5
88	9.5
89	6.0
90	4.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0034672704159932535
4	0.0
5	0.05291692091706695
6	0.0
7	0.00303265434713616
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.4487202295236434E-5
16-17	0.0
18-19	0.0
20-21	7.243724978362993E-5
22-23	4.829454339754914E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.5014358982212638E-5
46-47	8.583945406511165E-4
48-49	2.393997728147092E-4
50-51	7.855605146273678E-4
52-53	0.0013652727163279792
54-55	0.0029837080169370475
56-57	0.0027486948551618787
58-59	0.0064006169962226855
60-61	0.005499137003165361
62-63	0.006332368797373598
64-65	0.0037360163345131563
66-67	0.00615613801373163
68-69	0.006825871575030719
70-71	0.0031950990964994515
72-73	0.0024832940884847122
74-75	0.0034690534358933663
76-77	0.0020653834018123313
78-79	0.0
80-81	5.079044824321969E-4
82-83	1.7103901364513865E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.074425886321627E-5
92-93	6.094900334697098E-4
94-95	6.920052134414589E-5
96-97	1.2748190537770305E-5
98-99	6.577218252359445E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	96.0
20-21	489.0
22-23	1057.0
24-25	1300.0
26-27	2982.0
28-29	6738.0
30-31	18685.0
32-33	25405.0
34-35	34979.0
36-37	54054.0
38-39	61281.0
40-41	62791.0
42-43	72531.0
44-45	87002.0
46-47	87687.0
48-49	80283.0
50-51	72196.0
52-53	65190.0
54-55	69579.0
56-57	70372.0
58-59	76896.0
60-61	75062.0
62-63	73540.0
64-65	79272.0
66-67	84550.0
68-69	85382.0
70-71	85398.0
72-73	78538.0
74-75	80555.0
76-77	84858.0
78-79	88389.0
80-81	87974.0
82-83	83735.0
84-85	83843.0
86-87	86567.0
88-89	90050.0
90-91	92814.0
92-93	90602.0
94-95	97286.0
96-97	155499.0
98-99	861489.0
100-101	6856970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.41684695077171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.26342167607321	75.3533660593127
2	7.529488333527571	12.712313285380358
3	1.7643194576582133	4.468148568882058
4	0.6637476298382443	2.2412592832797014
5	0.2996327352430561	1.2647025376227081
6	0.15455342149608672	0.7828147516891959
7	0.09879885024391587	0.5838201193967002
8	0.06271840047830435	0.42355916913393804
9	0.03611979567875246	0.27442073373357523
>10	0.12399427539779913	1.6229244965876843
>50	0.002496077014701236	0.1423028252888952
>100	6.514714334507294E-4	0.08336771695240126
>500	3.858395730267603E-5	0.02750651309598651
>1k	1.929195942680225E-5	0.01949393964416497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9316269726981912E-5
2	0.0	9.658134863490956E-6	0.0	0.0	1.9316269726981912E-5
3	0.0	9.658134863490956E-6	0.0	0.0	1.9316269726981912E-5
4	0.0	5.7948809180945734E-5	0.0	9.658134863490956E-6	1.9316269726981912E-5
5	0.0	7.726507890792765E-5	0.0	9.658134863490956E-6	1.9316269726981912E-5
6	2.8974404590472867E-5	7.726507890792765E-5	0.0	9.658134863490956E-6	1.9316269726981912E-5
7	2.8974404590472867E-5	7.726507890792765E-5	0.0	9.658134863490956E-6	1.9316269726981912E-5
8	2.8974404590472867E-5	9.658134863490956E-5	0.0	1.9316269726981912E-5	2.8974404590472867E-5
9	3.8632539453963825E-5	1.1589761836189147E-4	0.0	1.9316269726981912E-5	2.8974404590472867E-5
10-11	3.8632539453963825E-5	1.1589761836189147E-4	0.0	9.175228120316408E-5	3.3803472022218344E-5
12-13	3.8632539453963825E-5	1.1589761836189147E-4	0.0	9.658134863490956E-5	3.8632539453963825E-5
14-15	3.8632539453963825E-5	1.786754949745827E-4	0.0	9.658134863490956E-5	4.34616068857093E-5
16-17	3.8632539453963825E-5	1.8350456240632816E-4	0.0	9.658134863490956E-5	4.829067431745478E-5
18-19	4.829067431745478E-5	2.0764989956505555E-4	0.0	1.1589761836189147E-4	6.277787661269121E-5
20-21	6.277787661269121E-5	2.414533715872739E-4	0.0	1.1589761836189147E-4	6.760694404443669E-5
22-23	6.760694404443669E-5	2.414533715872739E-4	0.0	1.4487202295236434E-4	7.243601147618217E-5
24-25	6.760694404443669E-5	2.5111150645076483E-4	0.0	1.8833362983807362E-4	9.658134863490956E-5
26-27	7.726507890792765E-5	2.607696413142558E-4	0.0	2.7525684360949225E-4	9.658134863490956E-5
28-29	8.209414633967313E-5	2.607696413142558E-4	0.0	7.002147776030943E-4	1.0623948349840052E-4
30-31	9.658134863490956E-5	3.283765853586925E-4	0.0	0.002110302467672774	1.1589761836189147E-4
32-33	9.658134863490956E-5	3.525219225174199E-4	0.0	0.005394068321259699	1.2072668579363695E-4
34-35	9.658134863490956E-5	4.0564166426662016E-4	0.0	0.009875442897919503	1.3521388808887338E-4
36-37	9.658134863490956E-5	4.635904734475659E-4	0.0	0.016814812797337757	1.4487202295236434E-4
38-39	1.0623948349840052E-4	4.925648780380388E-4	0.0	0.02898406272533636	1.4970109038410983E-4
40-41	1.0623948349840052E-4	5.698299569459664E-4	0.0	0.06088005311201525	1.545301578158553E-4
42-43	1.0623948349840052E-4	6.036334289681847E-4	0.0	0.07257122536427105	1.5935922524760077E-4
44-45	1.0623948349840052E-4	6.857275753078579E-4	0.0	0.0887486012606184	1.786754949745827E-4
46-47	1.1589761836189147E-4	7.340182496253126E-4	0.0	0.10672239024157507	1.9316269726981912E-4
48-49	1.303848206571279E-4	7.340182496253126E-4	0.0	0.1233778438136652	2.0282083213331008E-4
50-51	1.6901736011109173E-4	8.257705308284767E-4	0.0	0.13972906613755542	2.0282083213331008E-4
52-53	1.786754949745827E-4	8.305995982602222E-4	0.0	0.16644829623740315	2.3179523672378296E-4
54-55	1.9316269726981912E-4	8.450868005554586E-4	0.0	0.22579270590612333	2.6559870874600126E-4
56-57	2.0282083213331008E-4	8.69232137714186E-4	0.0	0.29143904857327135	2.7525684360949225E-4
58-59	2.0282083213331008E-4	8.69232137714186E-4	0.0	0.36318933247414564	2.897440459047287E-4
60-61	2.2213710186029197E-4	9.078646771681499E-4	0.0	0.38252491847085457	2.897440459047287E-4
62-63	2.704277761777468E-4	9.464972166221137E-4	0.0	0.3989968674805384	3.283765853586925E-4
64-65	2.8008591104123774E-4	0.0010720529698474961	0.0	0.4073270088002994	3.283765853586925E-4
66-67	2.8491497847298324E-4	0.001091369239574478	0.0	0.4132571036064828	3.3320565279043796E-4
68-69	2.897440459047287E-4	0.0011493180487554236	0.0	0.418887796231898	3.3803472022218346E-4
70-71	2.994021807682196E-4	0.0011686343184824056	0.0	0.4239003682260498	3.525219225174199E-4
72-73	3.283765853586925E-4	0.0011686343184824056	0.0	0.4283141358586652	3.9598352940312917E-4
74-75	3.3803472022218346E-4	0.0011976087230728787	0.0	0.4317669190723632	4.0564166426662016E-4
76-77	3.4286378765392895E-4	0.0012748738019808061	0.0	0.4347947443520676	4.0564166426662016E-4
78-79	3.476928550856744E-4	0.00131350634143477	0.0	0.4377453045528641	4.0564166426662016E-4
80-81	3.476928550856744E-4	0.00131350634143477	0.0	0.4391360759732068	4.1047073169836565E-4
82-83	3.476928550856744E-4	0.00131350634143477	0.0	0.4396382989861083	4.152997991301111E-4
84-85	3.573509899491654E-4	0.00131350634143477	0.0	0.4401115475944194	4.152997991301111E-4
86-87	3.621800573809108E-4	0.0013328226111617518	0.0	0.4402853940219622	4.152997991301111E-4
88-89	3.670091248126563E-4	0.0013714551506157158	0.0	0.44036265910087014	4.297870014253475E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14430	0.0	52.083298	1
GTATCAA	27340	0.0	38.864418	1
TCAACGC	32960	0.0	31.713469	4
ATCAACG	33165	0.0	31.374178	3
CAACGCA	33940	0.0	30.835938	5
TATCAAC	34175	0.0	30.725008	2
AACGCAG	34925	0.0	30.02538	6
GTGGTAT	7040	0.0	26.443573	1
ACGCAGA	39900	0.0	26.270794	7
TGGTATC	6695	0.0	25.9998	2
CGCAGAG	41255	0.0	25.428133	8
GCAGAGT	46885	0.0	22.337866	9
GAGTACT	27955	0.0	20.724203	12-13
CAGAGTA	46050	0.0	19.4899	10-11
GTACATG	32465	0.0	19.384727	1
AGAGTAC	42820	0.0	18.984032	10-11
GTACTTT	30615	0.0	18.82518	14-15
TACATGG	32965	0.0	18.619009	2
ACATGGG	33110	0.0	17.976519	3
AGTACTT	29870	0.0	17.401073	12-13
>>END_MODULE