FastQCFastQC Report
Fri 27 May 2016
SRR2139496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11578970
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161950.049.6941
GTATCAA295150.038.5725361
TCAACGC359800.031.1235394
ATCAACG362550.030.781773
CAACGCA368950.030.3516715
TATCAAC370550.030.3154982
AACGCAG382450.029.3209656
GTGGTAT81900.028.7091461
TGGTATC77800.028.2776582
ACGCAGA436150.025.720797
CGCAGAG446350.025.1808648
GCAGAGT512900.021.837799
GAGTACT273250.020.09204912-13
GTACATG391550.019.5164261
CAGAGTA505050.018.93145810-11
TACATGG395800.018.8700562
AGAGTAC467700.018.65618510-11
ACATGGG396250.018.2159773
GTACTTT298950.018.14572314-15
AGTACTT301250.017.18501312-13
Produced by FastQC (version 0.11.5)