##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11578970
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.38386514517267	33.0	31.0	33.0	18.0	33.0
2	31.476272414558462	33.0	31.0	33.0	28.0	34.0
3	31.079289695024688	33.0	31.0	33.0	27.0	34.0
4	31.7412412330285	33.0	31.0	33.0	29.0	34.0
5	31.83168969260651	33.0	32.0	33.0	30.0	34.0
6	34.88462937549713	37.0	34.0	38.0	29.0	38.0
7	35.92951100141032	38.0	36.0	38.0	31.0	38.0
8	36.0493891080122	38.0	37.0	38.0	32.0	38.0
9	36.48530922871378	38.0	37.0	38.0	34.0	38.0
10-11	36.65044032413937	38.0	38.0	38.0	34.0	38.0
12-13	36.717465154499926	38.0	38.0	38.0	34.0	38.0
14-15	36.75680980259902	38.0	38.0	38.0	34.0	38.0
16-17	36.77095790903681	38.0	38.0	38.0	34.5	38.0
18-19	36.79643159106553	38.0	38.0	38.0	35.0	38.0
20-21	36.81428588823632	38.0	38.0	38.0	35.0	38.0
22-23	36.82894994291887	38.0	38.0	38.0	35.0	38.0
24-25	36.829726955916016	38.0	38.0	38.0	35.0	38.0
26-27	36.794191508254876	38.0	38.0	38.0	35.0	38.0
28-29	36.78975139116856	38.0	38.0	38.0	35.0	38.0
30-31	36.77083005640931	38.0	38.0	38.0	35.0	38.0
32-33	36.75324899960417	38.0	38.0	38.0	35.0	38.0
34-35	36.73260311388354	38.0	38.0	38.0	35.0	38.0
36-37	36.71144740336018	38.0	38.0	38.0	34.0	38.0
38-39	36.698857384666056	38.0	38.0	38.0	34.0	38.0
40-41	36.689770767538064	38.0	38.0	38.0	34.0	38.0
42-43	36.648011430238974	38.0	38.0	38.0	34.0	38.0
44-45	36.6056579956505	38.0	38.0	38.0	34.0	38.0
46-47	36.575077677780584	38.0	38.0	38.0	34.0	38.0
48-49	36.540216944009394	38.0	38.0	38.0	34.0	38.0
50-51	36.52682330465769	38.0	38.0	38.0	34.0	38.0
52-53	36.50327054790051	38.0	38.0	38.0	34.0	38.0
54-55	36.43437069023568	38.0	38.0	38.0	34.0	38.0
56-57	36.4419432634683	38.0	38.0	38.0	34.0	38.0
58-59	36.43849086107779	38.0	38.0	38.0	34.0	38.0
60-61	36.39483398247593	38.0	38.0	38.0	34.0	38.0
62-63	36.39759585716027	38.0	38.0	38.0	34.0	38.0
64-65	36.41756084294755	38.0	38.0	38.0	34.0	38.0
66-67	36.39680200783708	38.0	38.0	38.0	34.0	38.0
68-69	36.38275507259871	38.0	38.0	38.0	34.0	38.0
70-71	36.38248399214876	38.0	38.0	38.0	34.0	38.0
72-73	36.36979677714544	38.0	38.0	38.0	34.0	38.0
74-75	36.35703060035938	38.0	38.0	38.0	34.0	38.0
76-77	36.3214705876229	38.0	38.0	38.0	33.0	38.0
78-79	36.3182578131764	38.0	38.0	38.0	33.0	38.0
80-81	36.3083563308394	38.0	37.5	38.0	33.0	38.0
82-83	36.27955456982738	38.0	37.0	38.0	33.0	38.0
84-85	36.23355543631489	38.0	37.0	38.0	33.0	38.0
86-87	36.24837132494818	38.0	37.0	38.0	33.0	38.0
88-89	36.24247770095029	38.0	37.0	38.0	33.0	38.0
90-91	36.23335308803492	38.0	37.0	38.0	33.0	38.0
92-93	36.217253315319724	38.0	37.0	38.0	33.0	38.0
94-95	36.19943301193855	38.0	37.0	38.0	33.0	38.0
96-97	36.191916597231014	38.0	37.0	38.0	33.0	38.0
98-99	36.2324869566076	38.0	37.0	38.0	33.0	38.0
100-101	35.702077065636146	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	5.0
19	17.0
20	87.0
21	516.0
22	2494.0
23	7493.0
24	17034.0
25	32174.0
26	53184.0
27	79073.0
28	111532.0
29	149408.0
30	193292.0
31	245014.0
32	312971.0
33	408808.0
34	563899.0
35	875972.0
36	1817164.0
37	6708830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.477279067136365	19.994559101543572	14.568307889216397	24.959853942103656
2	14.136542369485369	20.544754844342805	42.649311639981796	22.669391146190033
3	18.18517705712917	25.5092455005812	29.85307919790654	26.452498244383083
4	12.5015782923697	18.113027324537505	38.88927944368108	30.49611493941171
5	13.865021768800947	36.8121506071804	35.19982925414043	14.122998369878218
6	30.003307720807637	37.771908900359875	18.611793622403376	13.612989756429112
7	26.798964261028953	32.76598460624706	22.628647539472198	17.806403593251787
8	24.259748492309765	36.906063319967146	20.763081690340332	18.071106497382754
9	25.363352698901544	17.493956716357328	21.347045548956427	35.7956450357847
10-11	24.19175126076634	26.94808894694817	28.479391020073074	20.380768772212416
12-13	25.053365068454493	24.41760901088823	28.754596857691006	21.77442906296627
14-15	22.230364378728364	25.78710591590668	26.55332698446641	25.42920272089855
16-17	21.647689734060975	28.975547911429082	27.224856787779917	22.15190556673003
18-19	21.660739254009638	28.05224903424052	29.12569943613292	21.16131227561692
20-21	22.554670022800153	27.087031081798024	29.066442052900825	21.291856842501
22-23	22.311192798650083	26.961251633984812	28.91254111830558	21.81501444905953
24-25	21.993390048218327	27.125499844273694	29.010295913687305	21.87081419382067
26-27	21.904295262442155	27.288891693820226	28.96268932205866	21.844123721678958
28-29	21.832375408629318	27.28402862828172	29.037390007035935	21.846205956053026
30-31	22.104912609769766	27.201389473964944	28.863687588813104	21.83001032745219
32-33	21.880823535628487	27.376714039774598	28.832801311040374	21.909661113556542
34-35	22.062816756697664	27.31496205934437	28.831492016625166	21.7907291673328
36-37	22.03358078653928	27.20728060237874	28.805240025212825	21.953898585869158
38-39	21.8935950464504	27.38277643181487	28.869885945586176	21.85374257614855
40-41	22.132940226213364	27.260064321396797	28.742497822049913	21.864497630339923
42-43	22.01556648130059	27.34227558448795	28.572215723155626	22.069942211055835
44-45	22.061003933456806	27.423236743951257	28.46623950937042	22.049519813221515
46-47	22.127996033723296	27.434703429749202	28.20981766470997	22.22748287181754
48-49	22.069401626888872	27.51414194590842	28.227897330712263	22.188559096490433
50-51	22.097695555582803	27.627653112578095	28.092173551812653	22.182477780026453
52-53	22.29929215182498	27.661145995175435	27.802736028861542	22.236825824138048
54-55	22.288266799475533	27.575693401171385	27.812548533975278	22.323491265377804
56-57	22.225082620841043	27.65916054095094	27.842820380404397	22.27293645780362
58-59	22.158401689306885	27.63450489048641	27.915446769800546	22.291646650406165
60-61	22.266007035648443	27.64258718818613	27.8636037968142	22.227801979351227
62-63	22.214190004467742	27.71406112825533	27.903943717913513	22.167805149363417
64-65	22.16683549278565	27.65208964855957	27.867530777200223	22.313544081454555
66-67	22.22311517855982	27.758074076985416	27.67586017359658	22.34295057085819
68-69	22.142709886918713	27.731725890327724	27.789410762055823	22.33615346069774
70-71	22.18712749421784	27.488514068058034	27.839465219189556	22.48489321853457
72-73	22.220547761809975	27.55439438058897	27.711146975028957	22.513910882572095
74-75	22.12502483779017	27.696287427187855	27.6865726412482	22.49211509377377
76-77	22.31226264452913	27.5868156031254	27.638290960882205	22.46263079146327
78-79	22.260539636313208	27.584952498169514	27.56906214736835	22.58544571814893
80-81	22.246271125944105	27.74004048677927	27.49498344314786	22.518704944128764
82-83	22.332438957381	27.6636959787602	27.59040447511775	22.41346058874105
84-85	22.381831329899445	27.67073636422171	27.543437012374206	22.403995293504643
86-87	22.296546128827995	27.702026926552264	27.611346205303644	22.3900807393161
88-89	22.326932695199012	27.769227550631282	27.56939504788284	22.33444470628687
90-91	22.35936973549684	27.77618235963604	27.57279257370572	22.291655331161408
92-93	22.423526727985248	27.742182634895812	27.538059559377924	22.29623107774102
94-95	22.291579708209742	27.778396273598183	27.544918772813105	22.385105245378966
96-97	22.380250846831334	27.747150718234593	27.538349213887177	22.334249221046896
98-99	22.521925730445805	28.324733882383313	27.672683354294175	21.480657032876707
100-101	23.1433728414681	30.846385098707223	25.045352143849787	20.96488991597489
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2814.0
1	2467.5
2	2439.0
3	3128.0
4	4209.5
5	5946.5
6	7301.0
7	7615.5
8	7389.5
9	7290.0
10	7480.5
11	8746.0
12	11138.5
13	17171.5
14	27508.5
15	39307.0
16	49492.0
17	54814.5
18	55945.5
19	51688.0
20	46141.5
21	42711.5
22	41619.5
23	46048.5
24	55402.5
25	69955.5
26	88732.0
27	109448.5
28	132948.0
29	158617.0
30	183484.0
31	209893.5
32	239715.0
33	270835.0
34	299724.5
35	327153.5
36	358480.0
37	384921.0
38	404476.5
39	422177.0
40	437160.0
41	449988.5
42	457731.5
43	465497.5
44	475589.0
45	483352.0
46	484916.0
47	481435.0
48	479555.0
49	476554.0
50	468170.0
51	456294.5
52	438234.5
53	413282.0
54	387292.5
55	360019.0
56	329231.0
57	295992.0
58	264302.0
59	230899.0
60	193726.0
61	157313.0
62	124345.0
63	96446.0
64	72781.5
65	53992.5
66	39992.0
67	30425.5
68	23300.0
69	17280.0
70	12642.5
71	9025.0
72	6135.0
73	4062.0
74	2632.5
75	1613.5
76	1001.5
77	601.5
78	381.5
79	242.5
80	167.0
81	115.0
82	78.0
83	54.5
84	38.5
85	27.5
86	17.5
87	14.0
88	9.0
89	4.0
90	5.0
91	4.5
92	3.5
93	3.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0036186292908609316
4	0.0
5	0.053674895089977775
6	0.0
7	0.0032559027270992152
8	0.0
9	0.0
10-11	4.31817337811567E-6
12-13	4.31817337811567E-6
14-15	8.63634675623134E-6
16-17	0.0
18-19	0.0
20-21	4.319272130082309E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	8.1349589693007E-4
48-49	1.595022290892236E-4
50-51	7.714930029028801E-4
52-53	0.0014464310773505446
54-55	0.003173274606597947
56-57	0.0030175067563715336
58-59	0.006837221245329773
60-61	0.0059749996850239784
62-63	0.006723524211684822
64-65	0.003901800833763945
66-67	0.006799367575717508
68-69	0.006904347174247968
70-71	0.0035535322209815277
72-73	0.0026664554927652434
74-75	0.0037226808407444228
76-77	0.0021975692431070682
78-79	0.0
80-81	4.5332481707952834E-4
82-83	1.3155407672402143E-4
84-85	0.0
86-87	0.0
88-89	5.419921126055821E-6
90-91	2.191758844281178E-5
92-93	6.539876564817881E-4
94-95	8.969115635108123E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1371.0
20-21	4392.0
22-23	2618.0
24-25	2803.0
26-27	4503.0
28-29	9052.0
30-31	22838.0
32-33	28960.0
34-35	40435.0
36-37	62052.0
38-39	68985.0
40-41	69312.0
42-43	79835.0
44-45	94515.0
46-47	93674.0
48-49	85948.0
50-51	105643.0
52-53	68990.0
54-55	75593.0
56-57	76523.0
58-59	83634.0
60-61	81334.0
62-63	79359.0
64-65	85353.0
66-67	89863.0
68-69	91837.0
70-71	91111.0
72-73	85328.0
74-75	87089.0
76-77	91037.0
78-79	95103.0
80-81	95205.0
82-83	90594.0
84-85	90162.0
86-87	94536.0
88-89	98552.0
90-91	104570.0
92-93	100258.0
94-95	107534.0
96-97	170595.0
98-99	969253.0
100-101	7698621.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.37666784083744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3173884284878	75.36303615841574
2	7.456818734349266	12.583630349950436
3	1.797402397990327	4.549764753346635
4	0.6500336361166292	2.193906887999385
5	0.2986797890907842	1.260080267744224
6	0.14959502162412772	0.7573397670133151
7	0.1029884187963037	0.6082873722960144
8	0.06103720736427156	0.41200929373659667
9	0.037315532361578654	0.2833704251439248
>10	0.1246208655867891	1.6426814663005118
>50	0.0030956164446197076	0.16978721947469427
>100	9.717376817872717E-4	0.12934673227694513
>500	1.754171407918765E-5	0.007948198122513656
>1k	3.5072391630033564E-5	0.038811108179127876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.63634675623134E-6	0.0	0.0	0.0
3	0.0	1.727269351246268E-5	0.0	0.0	0.0
4	0.0	6.045442729361938E-5	0.0	8.63634675623134E-6	0.0
5	0.0	6.045442729361938E-5	0.0	1.727269351246268E-5	0.0
6	0.0	6.045442729361938E-5	0.0	2.590904026869402E-5	0.0
7	0.0	6.045442729361938E-5	0.0	3.454538702492536E-5	0.0
8	0.0	7.772712080608207E-5	0.0	3.454538702492536E-5	0.0
9	0.0	1.2090885458723876E-4	0.0	3.454538702492536E-5	0.0
10-11	0.0	1.2090885458723876E-4	0.0	3.8863560403041027E-5	0.0
12-13	1.727269351246268E-5	1.2090885458723876E-4	0.0	4.31817337811567E-5	8.63634675623134E-6
14-15	1.727269351246268E-5	1.554542416121641E-4	0.0	4.31817337811567E-5	4.3181733781156705E-5
16-17	4.31817337811567E-5	1.6409058836839546E-4	0.0	4.31817337811567E-5	5.181808053738804E-5
18-19	4.31817337811567E-5	1.8136328188085815E-4	0.0	5.181808053738804E-5	5.6136253915503714E-5
20-21	4.31817337811567E-5	1.986359753933208E-4	0.0	7.340894742796639E-5	6.045442729361938E-5
22-23	4.31817337811567E-5	2.2022684228389916E-4	0.0	8.636346756231341E-5	6.909077404985072E-5
24-25	6.045442729361938E-5	2.677267494431715E-4	0.0	1.1227250783100743E-4	8.204529418419773E-5
26-27	6.477260067173506E-5	2.849994429556342E-4	0.0	1.8136328188085815E-4	8.63634675623134E-5
28-29	6.909077404985072E-5	2.849994429556342E-4	0.0	6.218169664486564E-4	9.93179876966604E-5
30-31	7.772712080608207E-5	3.3249935011490657E-4	0.0	0.0018956781129927792	1.1227250783100741E-4
32-33	8.63634675623134E-5	3.3681752349302226E-4	0.0	0.00509544458617649	1.295452013434701E-4
34-35	9.93179876966604E-5	4.015901241647573E-4	0.0	0.00934452719024231	1.3818154809970145E-4
36-37	1.1227250783100741E-4	4.663627248364924E-4	0.0	0.015454742520275982	1.3818154809970145E-4
38-39	1.1227250783100741E-4	4.663627248364924E-4	0.0	0.026202676058405885	1.4681789485593278E-4
40-41	1.295452013434701E-4	5.311353255082274E-4	0.0	0.056330571717518915	1.4681789485593278E-4
42-43	1.295452013434701E-4	5.440898456425744E-4	0.0	0.06645237011582206	1.5113606823404847E-4
44-45	1.295452013434701E-4	6.045442729361939E-4	0.0	0.08075847851751927	1.597724149902798E-4
46-47	1.3818154809970145E-4	6.649987002298132E-4	0.0	0.09693435599194056	1.6840876174651113E-4
48-49	1.5113606823404847E-4	6.693168736079289E-4	0.0	0.11151682748983718	1.8999962863708948E-4
50-51	1.5545424161216413E-4	7.945439015732832E-4	0.0	0.12556816366222556	1.9431780201520515E-4
52-53	1.6840876174651113E-4	7.945439015732832E-4	0.0	0.14870925479554745	2.1159049552766783E-4
54-55	1.8568145525897382E-4	8.11816595085746E-4	0.0	0.20408982836988093	2.2454501566201483E-4
56-57	1.8999962863708948E-4	8.3772563535444E-4	0.0	0.2660728890393532	2.288631890401305E-4
58-59	1.8999962863708948E-4	8.593165022450183E-4	0.0	0.33562570764066235	2.3749953579636185E-4
60-61	1.8999962863708948E-4	8.852255425137123E-4	0.0	0.35414635325940047	2.5045405593070885E-4
62-63	1.8999962863708948E-4	9.068164094042907E-4	0.0	0.3655549673243821	2.5477222930882454E-4
64-65	2.029541487714365E-4	0.001023407090613414	0.0	0.3735133608602492	2.590904026869402E-4
66-67	2.2022684228389916E-4	0.001036361610747761	0.0	0.3791615316388245	2.590904026869402E-4
68-69	2.2454501566201483E-4	0.0010795433445289176	0.0	0.38455493018809095	2.6340857606505587E-4
70-71	2.288631890401305E-4	0.0011054523847976116	0.0	0.38950787505278966	2.849994429556342E-4
72-73	2.331813624182462E-4	0.0011097705581757274	0.0	0.3935842307217309	3.540902170054849E-4
74-75	2.331813624182462E-4	0.0011356795984444213	0.0	0.3971769509723231	3.540902170054849E-4
76-77	2.4181770917447752E-4	0.0012090885458723877	0.0	0.40033353571172564	3.540902170054849E-4
78-79	2.4181770917447752E-4	0.0012393157595191975	0.0	0.40317057562114766	3.540902170054849E-4
80-81	2.4181770917447752E-4	0.001243633932897313	0.0	0.4045351184086322	3.7568108389606327E-4
82-83	2.4181770917447752E-4	0.001243633932897313	0.0	0.4049798902665781	3.972719507866416E-4
84-85	2.4181770917447752E-4	0.001243633932897313	0.0	0.4054376166446584	3.972719507866416E-4
86-87	2.4181770917447752E-4	0.0012522702796535442	0.0	0.4056017072330268	3.972719507866416E-4
88-89	2.4181770917447752E-4	0.0013559064407283204	0.0	0.4056578434869423	4.05908297542873E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16195	0.0	49.694	1
GTATCAA	29515	0.0	38.572536	1
TCAACGC	35980	0.0	31.123539	4
ATCAACG	36255	0.0	30.78177	3
CAACGCA	36895	0.0	30.351671	5
TATCAAC	37055	0.0	30.315498	2
AACGCAG	38245	0.0	29.320965	6
GTGGTAT	8190	0.0	28.709146	1
TGGTATC	7780	0.0	28.277658	2
ACGCAGA	43615	0.0	25.72079	7
CGCAGAG	44635	0.0	25.180864	8
GCAGAGT	51290	0.0	21.83779	9
GAGTACT	27325	0.0	20.092049	12-13
GTACATG	39155	0.0	19.516426	1
CAGAGTA	50505	0.0	18.931458	10-11
TACATGG	39580	0.0	18.870056	2
AGAGTAC	46770	0.0	18.656185	10-11
ACATGGG	39625	0.0	18.215977	3
GTACTTT	29895	0.0	18.145723	14-15
AGTACTT	30125	0.0	17.185013	12-13
>>END_MODULE