FastQCFastQC Report
Fri 27 May 2016
SRR2139495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11815044
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170250.051.8697131
GTATCAA321300.039.472061
ATCAACG388750.032.0990873
TCAACGC390150.032.082464
CAACGCA396950.031.478245
TATCAAC402750.031.1771342
AACGCAG410850.030.4796416
ACGCAGA463450.026.9922477
CTTATAC194600.026.9244771
CGCAGAG474200.026.4798778
GTGGTAT83550.024.4510561
TGGTATC80900.023.804422
GCAGAGT543200.023.0683949
TATACAC245450.022.9899183
TTATACA250600.021.6174562
GAGTACT340000.021.00909412-13
CAGAGTA532650.019.96517810-11
AGAGTAC500900.019.10199410-11
GTACTTT372500.019.01956214-15
ACACATC285700.018.7826126
Produced by FastQC (version 0.11.5)