##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11815044
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.468526058811122	33.0	31.0	33.0	18.0	33.0
2	31.489551118049157	33.0	31.0	33.0	28.0	34.0
3	31.091978921111085	33.0	31.0	33.0	27.0	34.0
4	31.7212584227363	33.0	31.0	33.0	29.0	34.0
5	31.81511985905427	33.0	32.0	33.0	30.0	34.0
6	34.8690451766409	37.0	34.0	38.0	29.0	38.0
7	35.88727007703061	38.0	36.0	38.0	31.0	38.0
8	36.006065064167345	38.0	37.0	38.0	31.0	38.0
9	36.45077987013845	38.0	37.0	38.0	34.0	38.0
10-11	36.60301722109541	38.0	38.0	38.0	34.0	38.0
12-13	36.668646896279014	38.0	38.0	38.0	34.0	38.0
14-15	36.70685276330752	38.0	38.0	38.0	34.0	38.0
16-17	36.71579538764308	38.0	38.0	38.0	34.0	38.0
18-19	36.74402537138245	38.0	38.0	38.0	34.0	38.0
20-21	36.76647492166889	38.0	38.0	38.0	34.5	38.0
22-23	36.781742460567244	38.0	38.0	38.0	35.0	38.0
24-25	36.77992830985365	38.0	38.0	38.0	35.0	38.0
26-27	36.738846916418495	38.0	38.0	38.0	34.0	38.0
28-29	36.72616100164562	38.0	38.0	38.0	34.0	38.0
30-31	36.698095694521314	38.0	38.0	38.0	34.0	38.0
32-33	36.67473365460256	38.0	38.0	38.0	34.0	38.0
34-35	36.64007336090574	38.0	38.0	38.0	34.0	38.0
36-37	36.610439912664646	38.0	38.0	38.0	34.0	38.0
38-39	36.557469939474586	38.0	38.0	38.0	34.0	38.0
40-41	36.595946209846204	38.0	38.0	38.0	34.0	38.0
42-43	36.56261619591417	38.0	38.0	38.0	34.0	38.0
44-45	36.51332405111539	38.0	38.0	38.0	34.0	38.0
46-47	36.46692596923316	38.0	38.0	38.0	34.0	38.0
48-49	36.42111620708369	38.0	38.0	38.0	34.0	38.0
50-51	36.39935722190539	38.0	38.0	38.0	34.0	38.0
52-53	36.36812267980015	38.0	38.0	38.0	34.0	38.0
54-55	36.295514478983975	38.0	38.0	38.0	33.0	38.0
56-57	36.30562925155358	38.0	38.0	38.0	33.0	38.0
58-59	36.29441916376767	38.0	38.0	38.0	33.0	38.0
60-61	36.25172225691195	38.0	37.5	38.0	33.0	38.0
62-63	36.25672601890476	38.0	37.5	38.0	33.0	38.0
64-65	36.277028739202194	38.0	38.0	38.0	33.0	38.0
66-67	36.25561507420606	38.0	37.5	38.0	33.0	38.0
68-69	36.23986435697039	38.0	37.0	38.0	33.0	38.0
70-71	36.240628253180944	38.0	37.0	38.0	33.0	38.0
72-73	36.2260130034227	38.0	37.0	38.0	33.0	38.0
74-75	36.21541642988393	38.0	37.0	38.0	33.0	38.0
76-77	36.18248240806746	38.0	37.0	38.0	33.0	38.0
78-79	36.18085251999848	38.0	37.0	38.0	33.0	38.0
80-81	36.17359397229028	38.0	37.0	38.0	33.0	38.0
82-83	36.144099328381785	38.0	37.0	38.0	33.0	38.0
84-85	36.107486319774495	38.0	37.0	38.0	33.0	38.0
86-87	36.124694851364154	38.0	37.0	38.0	33.0	38.0
88-89	36.127893169398156	38.0	37.0	38.0	33.0	38.0
90-91	36.11513852905398	38.0	37.0	38.0	33.0	38.0
92-93	36.10878097368251	38.0	37.0	38.0	33.0	38.0
94-95	36.09183741452596	38.0	37.0	38.0	33.0	38.0
96-97	36.08520298061852	38.0	37.0	38.0	33.0	38.0
98-99	36.13598639391367	38.0	37.0	38.0	33.0	38.0
100-101	35.609452011079526	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	9.0
19	20.0
20	92.0
21	683.0
22	2877.0
23	8650.0
24	19373.0
25	36441.0
26	60322.0
27	90591.0
28	127013.0
29	167773.0
30	215339.0
31	272388.0
32	343284.0
33	442529.0
34	604958.0
35	922936.0
36	1881670.0
37	6618092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09330815864926	20.17193503468967	14.542358030998445	25.19239877566262
2	13.91707893766625	20.249048585853764	43.274472782327344	22.559399694152642
3	18.061751155116127	25.5701731406691	30.06512358889792	26.302952115316852
4	12.20804594549119	18.428522145156634	38.696411117893426	30.667020791458754
5	13.6593053819439	36.819673660992265	35.370631115215026	14.150389841848805
6	29.70334261979896	38.00245686770189	18.42476422432282	13.86943628817633
7	26.371222858390702	32.83704009977439	22.369548666465505	18.4221883753694
8	24.016804338604242	37.17234569756998	20.688192104913025	18.122657858912756
9	24.90814253421316	17.518741360590788	21.19827061160331	36.374845493592744
10-11	24.057875876631346	26.97034505205165	28.415312224622784	20.556466846694217
12-13	25.008222567770378	24.25088725865092	28.69032057773124	22.050569595847463
14-15	22.097271338634624	25.664410432717442	26.4272007485373	25.811117480110628
16-17	21.619970268413727	29.077551467434233	27.050250511128016	22.252227753024027
18-19	21.58170972532984	27.792943471052666	29.159362419640587	21.465984383976902
20-21	22.291685137532212	26.84638792393678	29.177341680789166	21.68458525774184
22-23	22.065987716323846	27.036352677844196	29.054945663276737	21.84271394255522
24-25	22.015196807006205	27.128832545347926	29.20111748348989	21.654853164155973
26-27	21.949710235050972	27.22787616154801	29.120453426380084	21.701960177020936
28-29	21.627415912870653	27.055949952377347	29.21728170972328	22.099352425028723
30-31	22.217613851473782	27.02847313236249	29.15636477490834	21.59754824125539
32-33	22.14313032579697	26.966167392611272	29.290710606011377	21.59999167558038
34-35	21.71507514144106	27.085062369635704	29.540948206488704	21.658914282434534
36-37	21.935396303836324	27.120455560722316	29.190994582419265	21.753153553022088
38-39	21.655250841362825	27.06339065823869	29.400592687924988	21.8807658124735
40-41	21.88321152173375	26.918209100022946	29.364255859721165	21.834323518522144
42-43	21.967295593108457	26.924029405222814	29.23688050339626	21.87179449827246
44-45	21.787645086669148	27.303509869976132	29.031877101984787	21.876967941369934
46-47	22.082885856674643	27.026306105400245	28.568372150967104	22.322435886958008
48-49	22.020856801912498	27.0774909655714	28.561297590269675	22.340354642246428
50-51	21.804770373607127	27.300844042481554	28.665673059497138	22.22871252441418
52-53	21.946836022763236	27.377566823671323	28.716154851736604	21.95944230182884
54-55	21.944525815860278	27.357274094599088	28.418917598563237	22.279282490977394
56-57	22.14003700142719	27.570086143833255	28.17712135779787	22.112755496941688
58-59	21.773153330324803	27.880570757972606	28.60037355915006	21.74590235255253
60-61	21.9878035303455	28.04664398006086	28.109997213365162	21.85555527622848
62-63	21.85288838971203	28.37633599881641	28.09160761369685	21.679167997774705
64-65	21.6391048242677	28.281898481967765	28.078296330592874	22.000700363171664
66-67	21.7298616655709	28.25379326486246	27.94617444869764	22.070170620868996
68-69	21.735457946525813	28.21635013932054	28.100702023465285	21.947489890688367
70-71	21.83597105590928	27.704695811062063	28.238157536198077	22.221175596830577
72-73	21.972163608600955	27.542140715675995	28.09879592066861	22.386899755054436
74-75	21.8870735941663	27.648880358082767	28.084412235302686	22.379633812448247
76-77	22.014796150655798	27.42687431005557	28.12582341164657	22.432506127642057
78-79	21.97250645647225	27.442794953557755	28.070763247983262	22.513935341986738
80-81	22.16711702143827	27.487839709585344	27.90413006406418	22.440913204912206
82-83	22.245481212481344	27.655459401405135	27.9896119650212	22.10944742109232
84-85	22.164182273530443	27.517369346676418	27.85093434034805	22.467514039445092
86-87	22.04546077037702	27.70700257400069	27.913182935531644	22.33435372009065
88-89	22.323273549934182	27.524041824132862	27.85140716368288	22.30127746225008
90-91	22.327747364935473	27.737518514045927	27.835556014509976	22.099178106508624
92-93	22.169266836321597	27.66662358706035	27.910376518455028	22.25373305816302
94-95	22.206160342694446	27.58499101921427	27.920417562120235	22.288431075971047
96-97	22.212438222021415	27.788527425322627	27.937766842848568	22.06126750980739
98-99	22.519168391109286	28.20207047127164	28.052309042920093	21.226452094698978
100-101	22.9148014202373	30.79949644234146	25.50950586654347	20.776196270877765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3089.0
1	2897.0
2	3554.5
3	4711.0
4	5990.0
5	8598.0
6	11126.5
7	14394.0
8	18167.0
9	19760.0
10	18719.0
11	17399.5
12	18498.5
13	22955.0
14	32694.0
15	44788.0
16	54116.0
17	59404.5
18	59534.0
19	55052.5
20	49402.5
21	44346.5
22	42550.0
23	46378.5
24	55377.0
25	69853.5
26	88373.0
27	109185.5
28	132603.5
29	159119.0
30	185239.0
31	211970.5
32	241758.5
33	271588.0
34	299514.0
35	324750.5
36	352564.5
37	378670.5
38	397112.5
39	411393.0
40	425096.0
41	439497.5
42	448192.0
43	456481.5
44	467599.5
45	477418.5
46	504912.5
47	537476.5
48	536787.5
49	514179.5
50	493739.0
51	470486.5
52	442124.0
53	414950.0
54	387929.5
55	359611.5
56	329913.5
57	297314.0
58	264723.5
59	230088.5
60	190598.5
61	152865.5
62	119723.0
63	91565.0
64	68835.0
65	51121.5
66	37486.5
67	28202.5
68	21682.5
69	16180.5
70	11853.5
71	8456.0
72	5681.5
73	3616.5
74	2303.0
75	1479.0
76	935.5
77	565.0
78	354.5
79	239.0
80	160.5
81	100.5
82	69.0
83	45.5
84	26.5
85	15.5
86	11.0
87	9.5
88	7.5
89	5.5
90	3.5
91	2.0
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003647891620208947
4	0.0
5	0.05316103774137448
6	0.0
7	0.0032924126224159636
8	0.0
9	0.0
10-11	4.231892830868849E-6
12-13	0.0
14-15	1.2695678492606543E-5
16-17	0.0
18-19	0.0
20-21	5.924780092264908E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	8.761007000657864E-6
46-47	7.730923229060851E-4
48-49	1.6035444747068922E-4
50-51	6.417952602208252E-4
52-53	0.0012832033382083011
54-55	0.003064310929901523
56-57	0.002700159876923967
58-59	0.006308850829226781
60-61	0.005601660202458814
62-63	0.006160249079404444
64-65	0.0036225991283085563
66-67	0.006344799484627515
68-69	0.006675345095185815
70-71	0.0031899476931118833
72-73	0.0024730460066047996
74-75	0.003364098841775399
76-77	0.001990742399230765
78-79	0.0
80-81	5.13352501881869E-4
82-83	2.0012475469323337E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.1335686517318283E-5
92-93	6.739567580717374E-4
94-95	9.809696252565235E-5
96-97	5.517984518632275E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	616.0
22-23	1363.0
24-25	1574.0
26-27	3440.0
28-29	7936.0
30-31	21169.0
32-33	27236.0
34-35	38056.0
36-37	59064.0
38-39	68168.0
40-41	69282.0
42-43	79739.0
44-45	94738.0
46-47	94978.0
48-49	87091.0
50-51	77644.0
52-53	67691.0
54-55	71251.0
56-57	73755.0
58-59	46444.0
60-61	87783.0
62-63	85553.0
64-65	98162.0
66-67	108057.0
68-69	106978.0
70-71	126179.0
72-73	83704.0
74-75	83932.0
76-77	88851.0
78-79	93281.0
80-81	94556.0
82-83	89661.0
84-85	88491.0
86-87	92428.0
88-89	96871.0
90-91	101304.0
92-93	97912.0
94-95	106157.0
96-97	172880.0
98-99	1020579.0
100-101	7900343.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.87251553958413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74911209870109	70.96491431786913
2	8.121626101535929	13.13632666206313
3	2.1574099176498858	5.234255012711798
4	0.8149717161464567	2.6363525111350343
5	0.41733210209474053	1.6875348455912105
6	0.22617075969935904	1.0974598967031575
7	0.13798420548289464	0.7811390861492795
8	0.08720684530819857	0.5642109561876318
9	0.05649942560649173	0.41123256078047216
>10	0.22366912391094934	2.8876884926562227
>50	0.006244513764413121	0.34176180129387607
>100	0.0017081862806528393	0.20322222281173005
>500	2.166859107171359E-5	0.009795879487059405
>1k	4.333522785832343E-5	0.044105754560301136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	8.463785661737696E-6
4	0.0	4.231892830868848E-5	0.0	0.0	8.463785661737696E-6
5	0.0	4.231892830868848E-5	0.0	0.0	8.463785661737696E-6
6	1.0156542794085236E-4	4.231892830868848E-5	0.0	0.0	4.231892830868848E-5
7	1.0156542794085236E-4	4.231892830868848E-5	0.0	0.0	4.231892830868848E-5
8	1.0156542794085236E-4	8.463785661737696E-5	0.0	0.0	5.078271397042618E-5
9	1.0156542794085236E-4	1.5234814191127853E-4	0.0	1.6927571323475392E-5	5.924649963216388E-5
10-11	1.057973207717212E-4	1.5234814191127853E-4	0.0	1.6927571323475392E-5	5.924649963216388E-5
12-13	1.1002921360259006E-4	1.5234814191127853E-4	0.0	1.6927571323475392E-5	5.924649963216388E-5
14-15	1.1849299926432775E-4	1.94667070219967E-4	0.0	1.6927571323475392E-5	8.463785661737696E-5
16-17	1.8620328455822932E-4	2.0313085588170472E-4	0.0	1.6927571323475392E-5	1.0156542794085236E-4
18-19	1.8620328455822932E-4	2.200584272051801E-4	0.0	2.115946415434424E-5	1.057973207717212E-4
20-21	1.8620328455822932E-4	2.454497841903932E-4	0.0	2.9623249816081935E-5	1.1426110643345891E-4
22-23	1.9043517738909818E-4	2.750730340064752E-4	0.0	5.501460680129503E-5	1.3542057058780316E-4
24-25	1.94667070219967E-4	3.004643909916882E-4	0.0	9.733353510998352E-5	1.4388435624954085E-4
26-27	1.94667070219967E-4	3.1316006948429477E-4	0.0	1.481162490804097E-4	1.6081192757301624E-4
28-29	1.94667070219967E-4	3.1739196231516363E-4	0.0	4.8666767554991753E-4	1.6081192757301624E-4
30-31	1.94667070219967E-4	4.104936045942783E-4	0.0	0.001641974418377113	1.650438204038851E-4
32-33	1.94667070219967E-4	4.231892830868848E-4	0.0	0.004130327402927996	2.0313085588170472E-4
34-35	1.94667070219967E-4	4.951314612116552E-4	0.0	0.007266159990601813	2.285222128669178E-4
36-37	1.94667070219967E-4	5.58609853674688E-4	0.0	0.012230170281210971	2.285222128669178E-4
38-39	1.94667070219967E-4	5.755374249981634E-4	0.0	0.02259407582400878	2.3698599852865548E-4
40-41	1.94667070219967E-4	6.263201389685895E-4	0.0	0.05555628908364624	2.454497841903932E-4
42-43	1.94667070219967E-4	6.517114959538027E-4	0.0	0.06503572902479246	2.4968167702126205E-4
44-45	1.9889896305083586E-4	7.702044952181304E-4	0.0	0.07897558400967444	2.5814546268299976E-4
46-47	2.0313085588170472E-4	8.63306137497245E-4	0.0	0.093994571666428	2.920006053299505E-4
48-49	2.0313085588170472E-4	8.675380303281139E-4	0.0	0.10795981800829518	2.9623249816081935E-4
50-51	2.0736274871257355E-4	9.1832074429854E-4	0.0	0.12169654213729547	2.9623249816081935E-4
52-53	2.115946415434424E-4	9.352483156220155E-4	0.0	0.14648273844769433	3.089281766534259E-4
54-55	2.115946415434424E-4	9.394802084528843E-4	0.0	0.204832076799714	3.216238551460325E-4
56-57	2.115946415434424E-4	9.47943994114622E-4	0.0	0.269914356645646	3.2585574797690134E-4
58-59	2.115946415434424E-4	9.606396726072285E-4	0.0	0.33426028713900685	3.3855142646950785E-4
60-61	2.2852221286691782E-4	9.817991367615728E-4	0.0	0.6541786894741992	3.427833193003767E-4
62-63	2.623773555138686E-4	0.001007190493746786	0.0	0.6603953400427456	3.554789977929833E-4
64-65	2.623773555138686E-4	0.0011087559216876382	0.0	0.6664850338263658	3.554789977929833E-4
66-67	2.750730340064751E-4	0.0011214516001802448	0.0	0.6721684658982227	3.554789977929833E-4
68-69	2.79304926837344E-4	0.0011299153858419825	0.0	0.6772298097239418	3.597108906238521E-4
70-71	2.920006053299505E-4	0.0011341472786728514	0.0	0.6821472691934114	3.7240656911645864E-4
72-73	3.216238551460325E-4	0.0011383791715037202	0.0	0.6863495387744641	3.89334140439934E-4
74-75	3.216238551460325E-4	0.0011680024213198022	0.0	0.6897308211463283	3.89334140439934E-4
76-77	3.216238551460325E-4	0.0012187851352902282	0.0	0.6926169720569809	3.89334140439934E-4
78-79	3.216238551460325E-4	0.0012357127066137037	0.0	0.6950799336845466	3.89334140439934E-4
80-81	3.216238551460325E-4	0.0012357127066137037	0.0	0.6961548344635873	3.977979261016717E-4
82-83	3.2585574797690134E-4	0.0012357127066137037	0.0	0.6966118788893212	4.3588496157949137E-4
84-85	3.4701521213124556E-4	0.0012441764922754414	0.0	0.6970943146720401	4.401168544103602E-4
86-87	3.554789977929833E-4	0.00128649542058413	0.0	0.6972847498494292	4.443487472412291E-4
88-89	3.6394278345472093E-4	0.0013542057058780314	0.0	0.6973143730992454	4.570444257338356E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17025	0.0	51.869713	1
GTATCAA	32130	0.0	39.47206	1
ATCAACG	38875	0.0	32.099087	3
TCAACGC	39015	0.0	32.08246	4
CAACGCA	39695	0.0	31.47824	5
TATCAAC	40275	0.0	31.177134	2
AACGCAG	41085	0.0	30.479641	6
ACGCAGA	46345	0.0	26.992247	7
CTTATAC	19460	0.0	26.924477	1
CGCAGAG	47420	0.0	26.479877	8
GTGGTAT	8355	0.0	24.451056	1
TGGTATC	8090	0.0	23.80442	2
GCAGAGT	54320	0.0	23.068394	9
TATACAC	24545	0.0	22.989918	3
TTATACA	25060	0.0	21.617456	2
GAGTACT	34000	0.0	21.009094	12-13
CAGAGTA	53265	0.0	19.965178	10-11
AGAGTAC	50090	0.0	19.101994	10-11
GTACTTT	37250	0.0	19.019562	14-15
ACACATC	28570	0.0	18.782612	6
>>END_MODULE