FastQCFastQC Report
Fri 27 May 2016
SRR2139494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9798378
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143650.054.2793541
GTATCAA264000.037.6504251
TCAACGC317550.031.0372944
ATCAACG319700.030.6238353
TATCAAC325850.030.2748992
CAACGCA326000.030.2058685
AACGCAG335350.029.4124726
TGGTATC63750.026.8585012
GTGGTAT68550.026.1946871
ACGCAGA384450.025.5076947
CGCAGAG395050.024.855838
GCAGAGT444750.022.0289129
TATCTCG163050.021.36298694-95
GAGTACT266950.021.07888812-13
CAGAGTA440850.019.86506510-11
GTACTTT290850.019.24166914-15
GTACATG303550.019.0915451
AGAGTAC410800.018.56263410-11
TACATGG307100.018.5277442
ACATGGG305250.018.1366393
Produced by FastQC (version 0.11.5)