FastQCFastQC Report
Fri 27 May 2016
SRR2139493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10717355
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164350.052.334131
GTATCAA300750.038.381951
TCAACGC350400.033.2152564
ATCAACG352000.032.89233
CAACGCA360600.032.2636875
TATCAAC356800.032.0605962
AACGCAG371600.031.354586
GTGGTAT72650.028.014851
TGGTATC69600.027.6837392
ACGCAGA429200.027.1163757
CGCAGAG438400.026.6162198
GCAGAGT500050.023.3694679
CTTATAC113900.022.7839221
GAGTACT293250.021.7738112-13
CAGAGTA488500.020.61430510-11
GTACTTT320600.019.78805214-15
TATACAC157150.019.4958133
GTACATG360700.018.6241261
AGAGTAC457650.018.13675710-11
TACATGG361100.018.038662
Produced by FastQC (version 0.11.5)