##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10717355
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.69374934393794	30.0	18.0	33.0	18.0	33.0
2	27.857846362278753	29.0	25.0	32.0	18.0	33.0
3	27.66630413940753	29.0	25.0	31.0	18.0	33.0
4	30.810410964272435	33.0	30.0	33.0	27.0	33.0
5	31.694738860474438	33.0	32.0	33.0	30.0	33.0
6	35.76352085006049	38.0	36.0	38.0	31.0	38.0
7	36.02211375847865	38.0	36.0	38.0	32.0	38.0
8	36.46833542417882	38.0	37.0	38.0	34.0	38.0
9	36.710721815224	38.0	38.0	38.0	34.0	38.0
10-11	36.777162835419745	38.0	38.0	38.0	34.5	38.0
12-13	36.83355650717924	38.0	38.0	38.0	35.0	38.0
14-15	36.82922787385507	38.0	38.0	38.0	35.0	38.0
16-17	36.84309547458305	38.0	38.0	38.0	35.0	38.0
18-19	36.85761132294302	38.0	38.0	38.0	35.0	38.0
20-21	36.87278380604843	38.0	38.0	38.0	35.0	38.0
22-23	36.882811683151175	38.0	38.0	38.0	35.0	38.0
24-25	36.88023489562133	38.0	38.0	38.0	35.0	38.0
26-27	36.83154365835273	38.0	38.0	38.0	35.0	38.0
28-29	36.82202271033448	38.0	38.0	38.0	35.0	38.0
30-31	36.807019204607364	38.0	38.0	38.0	35.0	38.0
32-33	36.77981180422928	38.0	38.0	38.0	35.0	38.0
34-35	36.74768824022982	38.0	38.0	38.0	35.0	38.0
36-37	36.71800300644655	38.0	38.0	38.0	34.0	38.0
38-39	36.68811722647579	38.0	38.0	38.0	34.0	38.0
40-41	36.70658064276536	38.0	38.0	38.0	34.0	38.0
42-43	36.662408550752275	38.0	38.0	38.0	34.0	38.0
44-45	36.61300888255669	38.0	38.0	38.0	34.0	38.0
46-47	36.567021314405686	38.0	38.0	38.0	34.0	38.0
48-49	36.51906990962381	38.0	38.0	38.0	34.0	38.0
50-51	36.49675135413683	38.0	38.0	38.0	34.0	38.0
52-53	36.47060430320407	38.0	38.0	38.0	34.0	38.0
54-55	36.39966702832717	38.0	38.0	38.0	34.0	38.0
56-57	36.40575561362845	38.0	38.0	38.0	34.0	38.0
58-59	36.396978709032034	38.0	38.0	38.0	34.0	38.0
60-61	36.35621464939646	38.0	38.0	38.0	33.5	38.0
62-63	36.36369247508756	38.0	38.0	38.0	33.5	38.0
64-65	36.38767005873321	38.0	38.0	38.0	34.0	38.0
66-67	36.36481246604697	38.0	38.0	38.0	34.0	38.0
68-69	36.34602122791678	38.0	38.0	38.0	33.5	38.0
70-71	36.34115410030787	38.0	38.0	38.0	33.5	38.0
72-73	36.33147187863956	38.0	38.0	38.0	33.0	38.0
74-75	36.3213847631623	38.0	38.0	38.0	33.0	38.0
76-77	36.287183646239434	38.0	37.5	38.0	33.0	38.0
78-79	36.28505320850752	38.0	37.0	38.0	33.0	38.0
80-81	36.27750970133053	38.0	37.0	38.0	33.0	38.0
82-83	36.25053897410449	38.0	37.0	38.0	33.0	38.0
84-85	36.20989483135094	38.0	37.0	38.0	33.0	38.0
86-87	36.22437069072197	38.0	37.0	38.0	33.0	38.0
88-89	36.22175249694267	38.0	37.0	38.0	33.0	38.0
90-91	36.213728324040574	38.0	37.0	38.0	33.0	38.0
92-93	36.201588431278054	38.0	37.0	38.0	33.0	38.0
94-95	36.186695645259626	38.0	37.0	38.0	33.0	38.0
96-97	36.18426783336403	38.0	37.0	38.0	33.0	38.0
98-99	36.23027398574299	38.0	37.0	38.0	33.0	38.0
100-101	35.70745275177115	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	48.0
19	144.0
20	321.0
21	810.0
22	2616.0
23	6928.0
24	15598.0
25	29560.0
26	49156.0
27	74843.0
28	105992.0
29	142857.0
30	184439.0
31	232808.0
32	296606.0
33	386410.0
34	542131.0
35	872472.0
36	2077561.0
37	5696050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.61919848694011	18.235674753705556	13.529084368297962	21.616042391056375
2	14.937864799663721	20.05978154124782	40.59892576106698	24.40342789802148
3	20.215302365916795	25.219504056782448	29.33751567819471	25.227677899106048
4	13.19137044541307	17.928863978099074	38.592460546468786	30.28730503001907
5	14.07963353133066	37.86138805721051	34.1508050385335	13.908173372925331
6	30.33188692545875	37.14551771402552	18.429612530330477	14.092982830185246
7	27.009013548314858	32.87300787519128	21.993085880640464	18.124892695853394
8	24.315439770353787	36.85799341348682	20.517049215967933	18.309517600191466
9	25.3936255727276	17.709266885346246	21.07189693725737	35.82521060466878
10-11	24.4662465692328	26.823353336714145	28.037066981545355	20.673333112507702
12-13	25.337403678426252	24.167348193654124	28.39961912244206	22.095629005477562
14-15	22.5022545208216	25.531612044203072	26.196570888992664	25.76956254598266
16-17	21.950812490581864	28.846847006560854	26.8639557054889	22.33838479736838
18-19	21.93557085680189	27.720053128780375	28.788772976168094	21.555603038249643
20-21	22.72095510578268	26.77914228496182	28.891198792588042	21.608703816667465
22-23	22.436787364672163	26.823630949095502	28.73662818385545	22.002953502376887
24-25	22.256028990295196	26.948249747324155	28.91000820321577	21.88571305916488
26-27	22.223625399170395	27.05804289670605	28.86682976498591	21.851501939137652
28-29	22.183544648026267	26.998534601157687	28.880607365780197	21.937313385035846
30-31	22.325764586931896	27.03291197726152	28.8788481906043	21.762475245202282
32-33	22.235243689642466	27.186619228289565	28.696092634498697	21.882044447569264
34-35	22.397621789644305	27.05584977554708	28.78752067403586	21.759007760772754
36-37	22.152550738737023	27.11576211393042	28.749247036073562	21.982440111259
38-39	21.995403957311574	27.053858055702108	28.908474181520727	22.042263805465595
40-41	22.340611974773754	26.83587386725988	28.8028139248498	22.020700233116568
42-43	22.247935750148574	26.9329133040435	28.66659211280816	22.152558832999773
44-45	22.176225707675652	27.119524837515286	28.571129009344954	22.133120445464105
46-47	22.39334274431411	26.970531617391686	28.191816062220354	22.444309576073852
48-49	22.30697014075985	27.066722429438588	28.177344032496805	22.44896339730475
50-51	22.174498986201954	27.21767859713028	28.234437140401198	22.373385276266568
52-53	22.31490213781606	27.228258683341306	28.217448675181778	22.23939050366086
54-55	22.354926335375115	27.096834675091046	28.097797274628505	22.45044171490533
56-57	22.421157677199837	27.34047227665958	27.90608861121338	22.332281434927207
58-59	22.173245515710686	27.520778412489012	28.186401947247113	22.119574124553193
60-61	22.40055274817749	27.65110405180217	27.894753659848547	22.05358954017179
62-63	22.176814790542664	27.902167400076394	27.85039367163361	22.070624137747327
64-65	22.09592016189741	27.830876635619884	27.858553576437505	22.214649626045198
66-67	22.22843541527009	27.832100513590646	27.694463239731064	22.245000831408195
68-69	22.182832632892733	27.668288976789253	27.830305248752694	22.31857314156532
70-71	22.219647489508436	27.310617930337834	27.938807063314115	22.530927516839615
72-73	22.32047430547219	27.24321582524027	27.88103418214139	22.55527568714615
74-75	22.200729547318307	27.359235912046877	27.90095019341506	22.53908434721976
76-77	22.425657502599698	27.311348032793514	27.748915984125976	22.51407848048081
78-79	22.347321120044086	27.35363022522281	27.670647622914192	22.628401031818914
80-81	22.377427595944102	27.375899797408277	27.668529186981715	22.578143419665906
82-83	22.433862582976495	27.370026271780183	27.781119602828852	22.414991542414466
84-85	22.560232975891108	27.382215917281133	27.666581072881876	22.39097003394588
86-87	22.396854686343712	27.42899134708007	27.696291559808593	22.477862406767624
88-89	22.453492471811334	27.406550022847835	27.740772140946806	22.39918536439402
90-91	22.477665847952373	27.45576050857681	27.702962883135406	22.363610760335412
92-93	22.5586482466404	27.38333479543136	27.723546544740525	22.334470413187717
94-95	22.439690078216934	27.553684514072003	27.646488123754594	22.360137283956472
96-97	22.45091349772342	27.53530969623431	27.69695149139609	22.316825314646184
98-99	22.635607361921753	28.0659993132055	27.79509634228457	21.50329698258818
100-101	23.296255816864164	30.57411896422947	25.109646781994442	21.01997843691192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2278.0
1	1986.5
2	2381.5
3	3361.0
4	4076.5
5	5720.0
6	7839.5
7	10653.5
8	14988.0
9	17959.5
10	17014.5
11	15169.5
12	15666.0
13	18640.5
14	24933.5
15	32797.0
16	39825.5
17	45562.5
18	47514.5
19	45002.5
20	40671.5
21	36799.0
22	35651.5
23	38701.0
24	46072.0
25	58370.5
26	74918.0
27	93759.5
28	114840.0
29	137556.5
30	159908.5
31	182356.5
32	205640.5
33	229749.0
34	253393.5
35	275075.5
36	300002.0
37	324403.5
38	342741.5
39	359686.5
40	377501.5
41	393367.0
42	404098.5
43	416150.5
44	443724.5
45	463691.0
46	460031.5
47	453489.0
48	448393.5
49	443324.0
50	437911.0
51	429214.5
52	412427.5
53	391208.0
54	370502.0
55	346741.0
56	319622.5
57	290965.5
58	261262.0
59	228293.5
60	191111.5
61	154809.0
62	122278.0
63	94866.0
64	72654.5
65	55070.0
66	41821.5
67	31312.0
68	23300.0
69	17682.5
70	12748.5
71	8744.5
72	6053.0
73	4046.5
74	2562.5
75	1526.5
76	956.0
77	584.5
78	348.0
79	210.5
80	137.0
81	101.0
82	76.0
83	51.0
84	33.5
85	24.0
86	14.0
87	7.0
88	6.5
89	6.5
90	5.5
91	4.5
92	3.0
93	2.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0022486891588456293
4	0.0
5	0.03805976381299304
6	0.0
7	0.0014462523635729151
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.7323385158253254E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	5.734221645258015E-4
48-49	1.420518349104924E-4
50-51	4.045998263477282E-4
52-53	0.0011273230987956663
54-55	0.002918245988248763
56-57	0.002490243652985201
58-59	0.006529913691663655
60-61	0.005890161653802227
62-63	0.006612694872672714
64-65	0.0037775187577901606
66-67	0.00625347182716826
68-69	0.006272839365889862
70-71	0.0032866334378777997
72-73	0.002532329496369232
74-75	0.0036397268031926376
76-77	0.0022519801398364603
78-79	0.0
80-81	5.973815704993998E-4
82-83	2.4236940784024452E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.758029094678305E-5
92-93	6.871122443461176E-4
94-95	8.981565874935648E-5
96-97	0.0
98-99	6.253819520272006E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	532.0
22-23	1163.0
24-25	1223.0
26-27	2935.0
28-29	6695.0
30-31	17883.0
32-33	23810.0
34-35	33534.0
36-37	51791.0
38-39	58495.0
40-41	59112.0
42-43	67981.0
44-45	81554.0
46-47	83418.0
48-49	76372.0
50-51	67797.0
52-53	60752.0
54-55	66130.0
56-57	67757.0
58-59	73632.0
60-61	72909.0
62-63	72803.0
64-65	85109.0
66-67	92605.0
68-69	90178.0
70-71	101760.0
72-73	76490.0
74-75	77173.0
76-77	82096.0
78-79	86119.0
80-81	86097.0
82-83	81724.0
84-85	80882.0
86-87	84361.0
88-89	89314.0
90-91	91515.0
92-93	90156.0
94-95	96126.0
96-97	158617.0
98-99	910531.0
100-101	7208101.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.05216394226605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.65272322478666	73.62800503193318
2	7.801999653028862	12.959459085217118
3	1.9229091330589685	4.791052936946824
4	0.7054908681219141	2.343701729561312
5	0.34514899043095276	1.4332685268889553
6	0.18182425918103792	0.9060538909310781
7	0.1057283674564253	0.6146678795137427
8	0.07051897379713191	0.46854026982718117
9	0.045492172888899764	0.3400401060772933
>10	0.16307986290251555	2.0581926698467496
>50	0.0037291797754518546	0.20585564681846355
>100	0.0012948311921576387	0.1772865882439005
>500	2.016112698934263E-5	0.016526576960679926
>1k	4.03222521385257E-5	0.05734906123349021
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.799198123044352E-5
2	0.0	2.799198123044352E-5	0.0	0.0	2.799198123044352E-5
3	0.0	2.799198123044352E-5	0.0	0.0	2.799198123044352E-5
4	0.0	1.2129858533192192E-4	0.0	9.33066041014784E-6	2.799198123044352E-5
5	0.0	1.3062924574206976E-4	0.0	9.33066041014784E-6	2.799198123044352E-5
6	8.397594369133056E-5	1.3062924574206976E-4	0.0	9.33066041014784E-6	3.732264164059136E-5
7	8.397594369133056E-5	1.3062924574206976E-4	0.0	9.33066041014784E-6	3.732264164059136E-5
8	8.397594369133056E-5	1.4929056656236544E-4	0.0	1.866132082029568E-5	4.66533020507392E-5
9	8.397594369133056E-5	3.545650955856179E-4	0.0	5.598396246088704E-5	5.598396246088704E-5
10-11	8.864127389640448E-5	3.545650955856179E-4	0.0	8.397594369133056E-5	5.598396246088704E-5
12-13	9.797193430655232E-5	3.545650955856179E-4	0.0	9.33066041014784E-5	6.064929266596096E-5
14-15	1.1196792492177408E-4	5.038556621479834E-4	0.0	9.797193430655232E-5	6.99799530761088E-5
16-17	1.1196792492177408E-4	5.318476433784268E-4	0.0	1.2129858533192192E-4	8.397594369133056E-5
18-19	1.16633255126848E-4	5.738356152240921E-4	0.0	1.632865571775872E-4	1.2596391553699584E-4
20-21	1.2596391553699584E-4	6.251542474799052E-4	0.0	1.9127853840803072E-4	1.3062924574206976E-4
22-23	1.3062924574206976E-4	6.904688703509402E-4	0.0	2.099398592283264E-4	1.5862122697251328E-4
24-25	1.4462523635729152E-4	7.604488234270489E-4	0.0	2.7525448209936125E-4	1.9594386861310464E-4
26-27	1.5862122697251328E-4	8.11767455682862E-4	0.0	3.8722240702113533E-4	2.0527452902325248E-4
28-29	1.5862122697251328E-4	8.257634462980838E-4	0.0	7.884408046574924E-4	2.0527452902325248E-4
30-31	1.5862122697251328E-4	0.0010030459940908927	0.0	0.0021367212339238554	2.0527452902325248E-4
32-33	1.7261721758773504E-4	0.0010170419847061146	0.0	0.004823951432046433	2.2393584984354816E-4
34-35	1.9594386861310464E-4	0.0011430059002431104	0.0	0.008612199558566456	2.2393584984354816E-4
36-37	2.099398592283264E-4	0.0012596391553699583	0.0	0.014695790145982848	2.4259717066384384E-4
38-39	2.1460518943340032E-4	0.0013062924574206976	0.0	0.025468037589498525	2.519278310739917E-4
40-41	2.1927051963847424E-4	0.0014742443448033586	0.0	0.05335271622522535	2.612584914841395E-4
42-43	2.33266510253696E-4	0.0015348936374693196	0.0	0.06349047876085097	2.7525448209936125E-4
44-45	2.3793184045876992E-4	0.0017028455248519806	0.0	0.07705725899720593	2.985811331247309E-4
46-47	2.612584914841395E-4	0.0018101481195686808	0.0	0.09228023145636213	3.3590377476532224E-4
48-49	2.612584914841395E-4	0.0018241441101839026	0.0	0.10737257466977626	3.732264164059136E-4
50-51	2.612584914841395E-4	0.0021553825547441507	0.0	0.12151785585156039	3.8255707681606144E-4
52-53	2.6592382168921346E-4	0.0021833745359745946	0.0	0.14379947291099343	4.05883727841431E-4
54-55	2.7058915189428736E-4	0.00220203585679489	0.0	0.1959531992735148	4.338757090718746E-4
56-57	2.7058915189428736E-4	0.0022300278380253335	0.0	0.254475101365962	4.4320636948202237E-4
58-59	2.7525448209936125E-4	0.0022533544890507035	0.0	0.31994834546396944	4.478716996870963E-4
60-61	2.9391580291965693E-4	0.002318669111921738	0.0	0.33755530165791836	4.5720236009724416E-4
62-63	3.219077841501005E-4	0.002337330432742034	0.0	0.3518965267083156	4.5720236009724416E-4
64-65	3.265731143551744E-4	0.002612584914841395	0.0	0.35938438168745923	4.6186769030231805E-4
66-67	3.265731143551744E-4	0.0026265809054566167	0.0	0.36468886213062834	4.7119835071246594E-4
68-69	3.312384445602483E-4	0.002659238216892134	0.0	0.36999800790400245	4.7586368091753984E-4
70-71	3.405691049703962E-4	0.0026825648679175036	0.0	0.3752325083940954	4.898596715327615E-4
72-73	3.452344351754701E-4	0.0027058915189428737	0.0	0.37939864826722636	5.22516982968279E-4
74-75	3.452344351754701E-4	0.0027572101511986868	0.0	0.3826317220993426	5.22516982968279E-4
76-77	3.452344351754701E-4	0.002841186094890017	0.0	0.38535627493910574	5.22516982968279E-4
78-79	3.452344351754701E-4	0.0028925047271458303	0.0	0.38761896008856667	5.22516982968279E-4
80-81	3.452344351754701E-4	0.002897170057350904	0.0	0.38881328462106557	5.551742944037964E-4
82-83	3.452344351754701E-4	0.002901835387555978	0.0	0.3892005070280867	5.971622662494618E-4
84-85	3.4989976538054397E-4	0.0029251620385813476	0.0	0.38958772943510783	6.064929266596096E-4
86-87	3.545650955856179E-4	0.0029391580291965695	0.0	0.38972302401105496	6.158235870697574E-4
88-89	3.545650955856179E-4	0.0031211059071944523	0.0	0.3898256612755666	6.251542474799052E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16435	0.0	52.33413	1
GTATCAA	30075	0.0	38.38195	1
TCAACGC	35040	0.0	33.215256	4
ATCAACG	35200	0.0	32.8923	3
CAACGCA	36060	0.0	32.263687	5
TATCAAC	35680	0.0	32.060596	2
AACGCAG	37160	0.0	31.35458	6
GTGGTAT	7265	0.0	28.01485	1
TGGTATC	6960	0.0	27.683739	2
ACGCAGA	42920	0.0	27.116375	7
CGCAGAG	43840	0.0	26.616219	8
GCAGAGT	50005	0.0	23.369467	9
CTTATAC	11390	0.0	22.783922	1
GAGTACT	29325	0.0	21.77381	12-13
CAGAGTA	48850	0.0	20.614305	10-11
GTACTTT	32060	0.0	19.788052	14-15
TATACAC	15715	0.0	19.495813	3
GTACATG	36070	0.0	18.624126	1
AGAGTAC	45765	0.0	18.136757	10-11
TACATGG	36110	0.0	18.03866	2
>>END_MODULE