FastQCFastQC Report
Fri 27 May 2016
SRR2139492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12413188
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202400.053.0279461
GTATCAA364900.037.71131
TCAACGC424900.032.4674874
ATCAACG423350.032.453293
CAACGCA431900.031.8909075
TATCAAC436750.031.2384382
AACGCAG448250.030.7266146
GTGGTAT90250.028.8752521
TGGTATC89050.027.0170142
ACGCAGA519450.026.4479987
CGCAGAG531750.025.917498
GCAGAGT605850.022.6614489
CTTATAC135250.021.7769761
GAGTACT356400.021.4332812-13
CAGAGTA597000.020.30209510-11
GTACTTT390300.019.55542414-15
TATACAC188450.019.1152653
GTACATG435200.019.013751
TACATGG432700.018.5203382
ACATGGG436800.018.1572553
Produced by FastQC (version 0.11.5)