##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12413188
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.33383567541231	30.0	18.0	32.0	18.0	33.0
2	27.515796586662507	29.0	25.0	32.0	18.0	33.0
3	27.332694469784876	29.0	25.0	31.0	18.0	33.0
4	30.69791523337921	32.0	30.0	33.0	27.0	33.0
5	31.6517701173945	33.0	32.0	33.0	29.0	33.0
6	35.74371241295951	38.0	36.0	38.0	31.0	38.0
7	36.01789991418804	38.0	36.0	38.0	32.0	38.0
8	36.474572607778114	38.0	37.0	38.0	34.0	38.0
9	36.7184872250384	38.0	38.0	38.0	34.0	38.0
10-11	36.78242196927977	38.0	38.0	38.0	34.5	38.0
12-13	36.83766043823714	38.0	38.0	38.0	35.0	38.0
14-15	36.831334061805876	38.0	38.0	38.0	35.0	38.0
16-17	36.841812554518626	38.0	38.0	38.0	35.0	38.0
18-19	36.8581779314065	38.0	38.0	38.0	35.0	38.0
20-21	36.87330641162285	38.0	38.0	38.0	35.0	38.0
22-23	36.88380073349019	38.0	38.0	38.0	35.0	38.0
24-25	36.876040695540055	38.0	38.0	38.0	35.0	38.0
26-27	36.82901344839894	38.0	38.0	38.0	35.0	38.0
28-29	36.81889298155858	38.0	38.0	38.0	35.0	38.0
30-31	36.80243751527503	38.0	38.0	38.0	35.0	38.0
32-33	36.77978289742438	38.0	38.0	38.0	35.0	38.0
34-35	36.74366520662629	38.0	38.0	38.0	35.0	38.0
36-37	36.71835532712505	38.0	38.0	38.0	34.0	38.0
38-39	36.68293349135388	38.0	38.0	38.0	34.0	38.0
40-41	36.70346588714526	38.0	38.0	38.0	34.0	38.0
42-43	36.661545008575814	38.0	38.0	38.0	34.0	38.0
44-45	36.60812003455515	38.0	38.0	38.0	34.0	38.0
46-47	36.55862174331722	38.0	38.0	38.0	34.0	38.0
48-49	36.50942541731508	38.0	38.0	38.0	34.0	38.0
50-51	36.48647885500198	38.0	38.0	38.0	34.0	38.0
52-53	36.46041435805135	38.0	38.0	38.0	34.0	38.0
54-55	36.39108288417168	38.0	38.0	38.0	34.0	38.0
56-57	36.397988627402064	38.0	38.0	38.0	34.0	38.0
58-59	36.38910054452795	38.0	38.0	38.0	34.0	38.0
60-61	36.34995164235161	38.0	38.0	38.0	33.5	38.0
62-63	36.35780048190303	38.0	38.0	38.0	33.5	38.0
64-65	36.38110395599486	38.0	38.0	38.0	34.0	38.0
66-67	36.358523500715606	38.0	38.0	38.0	34.0	38.0
68-69	36.33791141082695	38.0	38.0	38.0	33.5	38.0
70-71	36.33733017985821	38.0	38.0	38.0	33.5	38.0
72-73	36.32662371463805	38.0	38.0	38.0	33.0	38.0
74-75	36.31859447156122	38.0	38.0	38.0	33.0	38.0
76-77	36.286210840429106	38.0	37.5	38.0	33.0	38.0
78-79	36.284113826786154	38.0	37.0	38.0	33.0	38.0
80-81	36.2793589522671	38.0	37.0	38.0	33.0	38.0
82-83	36.25169995966198	38.0	37.0	38.0	33.0	38.0
84-85	36.21391356563784	38.0	37.0	38.0	33.0	38.0
86-87	36.23003192179041	38.0	37.0	38.0	33.0	38.0
88-89	36.229381758535396	38.0	37.0	38.0	33.0	38.0
90-91	36.217980923840784	38.0	37.0	38.0	33.0	38.0
92-93	36.20956340160171	38.0	37.0	38.0	33.0	38.0
94-95	36.197391575182905	38.0	37.0	38.0	33.0	38.0
96-97	36.19415136566373	38.0	37.0	38.0	33.0	38.0
98-99	36.239937018890316	38.0	37.0	38.0	33.0	38.0
100-101	35.712166770461415	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	56.0
19	208.0
20	473.0
21	959.0
22	3082.0
23	8219.0
24	18425.0
25	34699.0
26	57257.0
27	87251.0
28	124120.0
29	166745.0
30	213024.0
31	269929.0
32	343317.0
33	448775.0
34	629183.0
35	1020077.0
36	2458432.0
37	6528956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.84821497910126	18.37939617123337	13.63909094102176	21.133297908643613
2	14.736391650557456	20.215346774736677	40.81233604131348	24.23592553339239
3	20.028602391631704	25.695314279895477	29.627836997303135	24.64824633116968
4	12.905089329187636	18.347486560261554	39.16151112832578	29.585912982225032
5	13.740106991605927	38.22468172773529	34.37616073385014	13.659050546808645
6	29.557564100374538	37.76253932511133	18.774596823958518	13.905299750555619
7	26.50636089759888	33.21321759686677	22.416625500240553	17.863796005293793
8	24.020501421552627	37.00233976960632	20.881622029731606	18.095536779109445
9	25.238367452422377	17.79264118129847	21.41064809459101	35.558343271688145
10-11	24.09565633323431	27.077183036186312	28.37633363710973	20.450826993469647
12-13	25.063522161411	24.523165383589028	28.691740135239236	21.72157231976074
14-15	22.287942216280513	25.900024852606325	26.518016931190502	25.294015999922664
16-17	21.530041275456394	29.21157723543702	27.232645634626657	22.025735854479926
18-19	21.493024193301512	28.036423842126617	29.258164784098977	21.2123871804729
20-21	22.361418312692248	27.07455777171249	29.344038697792286	21.21998521780297
22-23	22.073013051772957	27.169375515450948	29.175451295648262	21.58216013712783
24-25	21.82132733562224	27.373710234474842	29.335246032376244	21.46971639752667
26-27	21.738078241799048	27.59082900887156	29.2288956944172	21.44219705491219
28-29	21.662701536700652	27.45895261155432	29.333455492361683	21.54489035938334
30-31	22.03913874319073	27.281089306939826	29.255894446511583	21.42387750335786
32-33	21.744686804601596	27.437416366293892	29.25203541688422	21.565861412220293
34-35	21.870328037279332	27.27254535506522	29.2939948983438	21.563131709311648
36-37	21.72915281059808	27.384668527881683	29.25233446555756	21.633844195962673
38-39	21.599577606183125	27.311473568216705	29.45253226931487	21.636416556285305
40-41	21.870692774450053	27.150175002485543	29.36537970399901	21.613752519065393
42-43	21.866773406487336	27.17255168771466	29.210456824020625	21.750218081777383
44-45	21.821756835795135	27.423137404823294	29.011509482180998	21.743596277200577
46-47	22.02831008419219	27.257415638677017	28.61525982096218	22.09901445616861
48-49	21.94191641867571	27.339806179636906	28.63622732772314	22.08205007396424
50-51	21.835404204534584	27.51364742813591	28.635915857567724	22.015032509761777
52-53	21.94266950447737	27.52242795431649	28.66679771324716	21.868104827958977
54-55	21.997768252773305	27.46025537469935	28.47575334175224	22.066223030775102
56-57	22.070428038875566	27.721113074304515	28.278616072632317	21.9298428141876
58-59	21.809098557818192	27.87125010036971	28.57574509235759	21.74390624945451
60-61	22.025836361447446	28.078768020284333	28.198723403264015	21.696672215004213
62-63	21.77375138421237	28.32125521520219	28.200490267523783	21.70450313306166
64-65	21.75529582627647	28.18622717177831	28.193506688725268	21.86497031321995
66-67	21.849922804259812	28.177381256588056	28.076729316442588	21.895966622709544
68-69	21.807783507274305	28.0594485309737	28.18824747153812	21.944520490213876
70-71	21.86297478545267	27.713485179599108	28.300999074765006	22.12254096018322
72-73	21.96789349556379	27.621266900724926	28.26705707588667	22.143782527824605
74-75	21.86898409914466	27.746488863270883	28.236059370469967	22.14846766711448
76-77	22.03185729893201	27.651479402549956	28.16735035607747	22.149312942440563
78-79	22.025079003257584	27.643650443713668	28.05472171481339	22.27654883821535
80-81	21.965211242545287	27.75089017015297	28.06821512414973	22.215683463152015
82-83	22.060515632108803	27.691162777362184	28.152107277949217	22.096214312579797
84-85	22.182092630110407	27.684507222862326	28.04607427765146	22.087325869375807
86-87	22.03534096295394	27.746128262659138	28.097269031181547	22.121261743205377
88-89	22.117389043399083	27.766756118830312	28.07487724337853	22.04097759439207
90-91	22.128250458322007	27.831062362084502	28.030970964368677	22.009716215224813
92-93	22.1958379778231	27.77410727275103	28.03812938588697	21.991925363538904
94-95	22.05845666501965	27.81453267550038	28.0724342618462	22.054576397633767
96-97	22.124255867015513	27.838777807800398	28.01509955620117	22.02186676898292
98-99	22.280163047355988	28.406589875343297	28.155362358274793	21.157884719025922
100-101	22.926464075518506	30.891790712317846	25.538260531752016	20.64348468041163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2552.0
1	2361.5
2	3057.5
3	4309.0
4	5299.0
5	7560.5
6	10218.5
7	13409.0
8	18128.5
9	21286.5
10	20389.0
11	18610.5
12	19687.5
13	23977.0
14	32753.0
15	43542.5
16	52669.0
17	59491.0
18	61417.0
19	57908.0
20	51935.5
21	46053.5
22	43522.5
23	46819.0
24	55674.5
25	70597.5
26	90205.0
27	112444.0
28	137857.5
29	165997.0
30	194216.0
31	224675.5
32	257424.5
33	289805.5
34	320723.5
35	349338.0
36	379793.5
37	407090.5
38	428687.0
39	448074.0
40	465246.0
41	479684.0
42	489116.5
43	502020.5
44	531453.5
45	549866.0
46	540589.0
47	524844.0
48	510645.5
49	498222.0
50	484909.0
51	467620.5
52	445903.0
53	421993.0
54	396578.0
55	368005.0
56	335163.0
57	299061.5
58	264902.0
59	229649.0
60	190551.5
61	153504.5
62	120341.0
63	92664.5
64	69337.0
65	50852.0
66	37744.0
67	28785.0
68	21990.0
69	16041.0
70	11699.0
71	8404.0
72	5671.5
73	3708.5
74	2429.5
75	1519.5
76	930.5
77	563.5
78	375.5
79	245.5
80	158.0
81	108.0
82	69.0
83	50.0
84	39.5
85	25.5
86	18.5
87	13.0
88	8.0
89	3.5
90	3.0
91	4.0
92	4.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0019334275771864568
4	0.0
5	0.03666262043239819
6	0.0
7	0.0012889517181243046
8	0.0
9	0.0
10-11	4.027974119138452E-6
12-13	4.027974119138452E-6
14-15	2.416784471483071E-5
16-17	0.0
18-19	0.0
20-21	2.0140326516584712E-5
22-23	8.056674191197528E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	5.155675193301143E-4
48-49	1.3519463676116237E-4
50-51	5.105951906017806E-4
52-53	9.804848407624113E-4
54-55	0.002803926944910976
56-57	0.002431896178060577
58-59	0.0062225347744130416
60-61	0.005388493456782282
62-63	0.006025266363479041
64-65	0.003563803160491259
66-67	0.005922204730617537
68-69	0.006233723654395999
70-71	0.0032454473534731017
72-73	0.002507671109617118
74-75	0.003443546604431765
76-77	0.002035454315994765
78-79	0.0
80-81	5.086386516814682E-4
82-83	2.1794018723580503E-4
84-85	0.0
86-87	0.0
88-89	4.980540033979237E-6
90-91	2.0127218107492024E-5
92-93	6.762972066891388E-4
94-95	1.2848550994382766E-4
96-97	0.0
98-99	5.364670159961052E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	169.0
20-21	640.0
22-23	1378.0
24-25	1533.0
26-27	3288.0
28-29	7800.0
30-31	19902.0
32-33	26804.0
34-35	37640.0
36-37	57382.0
38-39	64886.0
40-41	66906.0
42-43	77523.0
44-45	94190.0
46-47	95998.0
48-49	86690.0
50-51	75882.0
52-53	67841.0
54-55	73646.0
56-57	74427.0
58-59	81477.0
60-61	82772.0
62-63	82190.0
64-65	97816.0
66-67	104910.0
68-69	102648.0
70-71	118220.0
72-73	86072.0
74-75	87731.0
76-77	92771.0
78-79	97546.0
80-81	97161.0
82-83	92929.0
84-85	93152.0
86-87	97008.0
88-89	101504.0
90-91	104222.0
92-93	103155.0
94-95	111255.0
96-97	184131.0
98-99	1047769.0
100-101	8412224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.084719296372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.51679257126555	73.54392863798785
2	7.825309947781733	13.003273608371055
3	1.9802986843135355	4.935976809274946
4	0.7701785845905703	2.559602860351385
5	0.34984283867184635	1.4533297024448155
6	0.18755097226786718	0.9349571930782437
7	0.10312130192393415	0.5997463096678546
8	0.07266288340611048	0.4829740216849357
9	0.04804537242536227	0.3592652653305788
>10	0.14259212816094324	1.8148819399128342
>50	0.0027990859617133125	0.15954354111807229
>100	7.206789936577434E-4	0.0982183340332832
>500	5.097682330840196E-5	0.024014511277163905
>1k	3.397341390469627E-5	0.030287265466976764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6111896476553807E-5	0.0	8.055948238276904E-6	0.0
3	0.0	1.6111896476553807E-5	0.0	8.055948238276904E-6	0.0
4	0.0	5.6391637667938325E-5	0.0	8.055948238276904E-6	0.0
5	0.0	5.6391637667938325E-5	0.0	8.055948238276904E-6	0.0
6	6.444758590621523E-5	5.6391637667938325E-5	0.0	2.416784471483071E-5	0.0
7	6.444758590621523E-5	5.6391637667938325E-5	0.0	2.416784471483071E-5	0.0
8	6.444758590621523E-5	5.6391637667938325E-5	0.0	2.416784471483071E-5	0.0
9	6.444758590621523E-5	7.250353414449213E-5	0.0	2.416784471483071E-5	0.0
10-11	6.847556002535367E-5	7.250353414449213E-5	0.0	3.2223792953107615E-5	0.0
12-13	7.250353414449213E-5	7.250353414449213E-5	0.0	3.625176707224607E-5	0.0
14-15	7.250353414449213E-5	1.1278327533587665E-4	0.0	4.833568942966142E-5	0.0
16-17	1.1278327533587665E-4	1.168112494550151E-4	0.0	4.833568942966142E-5	0.0
18-19	1.1278327533587665E-4	1.2083922357415355E-4	0.0	4.833568942966142E-5	0.0
20-21	1.1278327533587665E-4	1.24867197693292E-4	0.0	5.6391637667938325E-5	0.0
22-23	1.1278327533587665E-4	1.5709099064639963E-4	0.0	6.847556002535367E-5	0.0
24-25	1.1278327533587665E-4	1.6514693888467651E-4	0.0	7.250353414449213E-5	0.0
26-27	1.1278327533587665E-4	1.6917491300381498E-4	0.0	1.6917491300381498E-4	0.0
28-29	1.1278327533587665E-4	1.6917491300381498E-4	0.0	5.478044802028294E-4	0.0
30-31	1.1278327533587665E-4	1.8528680948036878E-4	0.0	0.0016071616735362422	0.0
32-33	1.168112494550151E-4	1.8931478359950722E-4	0.0	0.0036855963190116836	0.0
34-35	1.2889517181243046E-4	2.1348262831433795E-4	0.0	0.006646157296578446	0.0
36-37	1.2889517181243046E-4	2.255665506717533E-4	0.0	0.011169572232370927	0.0
38-39	1.2889517181243046E-4	2.255665506717533E-4	0.0	0.02012778667333484	0.0
40-41	1.3695112005070737E-4	2.658462918631378E-4	0.0	0.04554833133921761	0.0
42-43	1.490350424081227E-4	2.739022401014147E-4	0.0	0.05426486733303322	0.0
44-45	1.5709099064639963E-4	2.9807008481624544E-4	0.0	0.06639712537987824	0.0
46-47	1.6111896476553807E-4	3.1418198129279926E-4	0.0	0.07966124415420116	0.0
48-49	1.6111896476553807E-4	3.1418198129279926E-4	0.0	0.09255478930956335	0.0
50-51	1.6111896476553807E-4	3.665456448415991E-4	0.0	0.10447759270221316	0.0
52-53	1.6111896476553807E-4	3.7057361896073755E-4	0.0	0.12460135140142886	0.0
54-55	1.6111896476553807E-4	3.7057361896073755E-4	0.0	0.17292495690873289	0.0
56-57	1.6514693888467651E-4	3.7057361896073755E-4	0.0	0.22748789432658234	0.0
58-59	1.6917491300381498E-4	3.8265754131815296E-4	0.0	0.2888017163681078	0.0
60-61	1.6917491300381498E-4	3.987694377947067E-4	0.0	0.3057111517202511	0.0
62-63	2.013987059569226E-4	4.027974119138452E-4	0.0	0.31532995391675367	0.0
64-65	2.013987059569226E-4	5.155806872497218E-4	0.0	0.32263267099475174	0.0
66-67	2.175106024334764E-4	5.155806872497218E-4	0.0	0.3275830511871729	0.0
68-69	2.255665506717533E-4	5.155806872497218E-4	0.0	0.3324528718972113	0.0
70-71	2.336224989100302E-4	5.196086613688602E-4	0.0	0.3371937974354372	0.0
72-73	2.4973439538658403E-4	5.236366354879988E-4	0.0	0.341145240046312	0.0
74-75	2.4973439538658403E-4	5.276646096071372E-4	0.0	0.3442387241698104	0.0
76-77	2.577903436248609E-4	5.397485319645525E-4	0.0	0.34683273950253557	0.0
78-79	2.577903436248609E-4	5.478044802028295E-4	0.0	0.34920118828458896	0.0
80-81	2.577903436248609E-4	5.478044802028295E-4	0.0	0.35028471332263716	0.0
82-83	2.577903436248609E-4	5.478044802028295E-4	0.0	0.35082848982872084	0.0
84-85	2.577903436248609E-4	5.478044802028295E-4	0.0	0.35122725926651555	0.0
86-87	2.6181831774399933E-4	5.478044802028295E-4	0.0	0.35141657405011506	0.0
88-89	2.7390224010141474E-4	5.800282731559371E-4	0.0	0.351472965687783	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20240	0.0	53.027946	1
GTATCAA	36490	0.0	37.7113	1
TCAACGC	42490	0.0	32.467487	4
ATCAACG	42335	0.0	32.45329	3
CAACGCA	43190	0.0	31.890907	5
TATCAAC	43675	0.0	31.238438	2
AACGCAG	44825	0.0	30.726614	6
GTGGTAT	9025	0.0	28.875252	1
TGGTATC	8905	0.0	27.017014	2
ACGCAGA	51945	0.0	26.447998	7
CGCAGAG	53175	0.0	25.91749	8
GCAGAGT	60585	0.0	22.661448	9
CTTATAC	13525	0.0	21.776976	1
GAGTACT	35640	0.0	21.43328	12-13
CAGAGTA	59700	0.0	20.302095	10-11
GTACTTT	39030	0.0	19.555424	14-15
TATACAC	18845	0.0	19.115265	3
GTACATG	43520	0.0	19.01375	1
TACATGG	43270	0.0	18.520338	2
ACATGGG	43680	0.0	18.157255	3
>>END_MODULE