FastQCFastQC Report
Fri 27 May 2016
SRR2139491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13763229
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203150.050.0927161
GTATCAA357100.036.1385841
TCAACGC413800.031.1121444
ATCAACG413700.030.9842553
CAACGCA423100.030.4282785
GTGGTAT101550.030.0373571
TGGTATC96250.030.021022
TATCAAC424100.030.019532
AACGCAG438050.029.468266
ACGCAGA509850.025.2928127
CGCAGAG525650.024.5897758
CGAGACC109450.022.20780688-89
GACCGAG115250.021.85731790-91
AGACCGA112900.021.76564890-91
GAGACCG115300.021.6339188-89
ACCGAGG118850.021.45306892-93
GCAGAGT607100.021.2406759
GAGTACT349350.020.83682812-13
ATCTCCG115300.020.27116674-75
ACGAGAC122700.020.22568386-87
Produced by FastQC (version 0.11.5)