##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13763229
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.641682050047994	30.0	18.0	32.0	18.0	33.0
2	27.76303860089809	29.0	25.0	32.0	18.0	33.0
3	27.560499211340595	29.0	25.0	31.0	18.0	33.0
4	30.73648058896644	32.0	30.0	33.0	27.0	33.0
5	31.63442648523831	33.0	32.0	33.0	29.0	33.0
6	35.71265318625448	38.0	36.0	38.0	31.0	38.0
7	35.97959025458343	38.0	36.0	38.0	31.0	38.0
8	36.43652278110028	38.0	37.0	38.0	34.0	38.0
9	36.68014330067457	38.0	38.0	38.0	34.0	38.0
10-11	36.7479430517359	38.0	38.0	38.0	34.0	38.0
12-13	36.807583634625274	38.0	38.0	38.0	35.0	38.0
14-15	36.7998541984588	38.0	38.0	38.0	35.0	38.0
16-17	36.81177215027084	38.0	38.0	38.0	35.0	38.0
18-19	36.82387305333654	38.0	38.0	38.0	35.0	38.0
20-21	36.83973514943132	38.0	38.0	38.0	35.0	38.0
22-23	36.84910455151945	38.0	38.0	38.0	35.0	38.0
24-25	36.84155975080256	38.0	38.0	38.0	35.0	38.0
26-27	36.79182151634721	38.0	38.0	38.0	35.0	38.0
28-29	36.78163381124545	38.0	38.0	38.0	35.0	38.0
30-31	36.7669024551445	38.0	38.0	38.0	35.0	38.0
32-33	36.750881986584176	38.0	38.0	38.0	35.0	38.0
34-35	36.72245036144392	38.0	38.0	38.0	34.5	38.0
36-37	36.693954369812054	38.0	38.0	38.0	34.0	38.0
38-39	36.677101371099944	38.0	38.0	38.0	34.0	38.0
40-41	36.66870548746789	38.0	38.0	38.0	34.0	38.0
42-43	36.621810098818116	38.0	38.0	38.0	34.0	38.0
44-45	36.57649648694762	38.0	38.0	38.0	34.0	38.0
46-47	36.54068520065229	38.0	38.0	38.0	34.0	38.0
48-49	36.50040508213051	38.0	38.0	38.0	34.0	38.0
50-51	36.48348890202803	38.0	38.0	38.0	34.0	38.0
52-53	36.46603341605041	38.0	38.0	38.0	34.0	38.0
54-55	36.39441444127837	38.0	38.0	38.0	34.0	38.0
56-57	36.398165278769405	38.0	38.0	38.0	34.0	38.0
58-59	36.391629339775335	38.0	38.0	38.0	34.0	38.0
60-61	36.3492571053914	38.0	38.0	38.0	33.5	38.0
62-63	36.35519190613806	38.0	38.0	38.0	33.5	38.0
64-65	36.378053147368746	38.0	38.0	38.0	34.0	38.0
66-67	36.358159297835826	38.0	38.0	38.0	34.0	38.0
68-69	36.34106918483651	38.0	38.0	38.0	33.5	38.0
70-71	36.33982800903972	38.0	38.0	38.0	33.0	38.0
72-73	36.33010057731738	38.0	38.0	38.0	33.0	38.0
74-75	36.31916365693203	38.0	37.5	38.0	33.0	38.0
76-77	36.28094566828782	38.0	37.0	38.0	33.0	38.0
78-79	36.276151474114975	38.0	37.0	38.0	33.0	38.0
80-81	36.2617575716055	38.0	37.0	38.0	33.0	38.0
82-83	36.22720539505463	38.0	37.0	38.0	33.0	38.0
84-85	36.18640250821689	38.0	37.0	38.0	33.0	38.0
86-87	36.20295951909554	38.0	37.0	38.0	33.0	38.0
88-89	36.19843040059704	38.0	37.0	38.0	33.0	38.0
90-91	36.18020658776212	38.0	37.0	38.0	33.0	38.0
92-93	36.16785466599521	38.0	37.0	38.0	33.0	38.0
94-95	36.14859231817219	38.0	37.0	38.0	33.0	38.0
96-97	36.13691554688701	38.0	37.0	38.0	33.0	38.0
98-99	36.18286239705782	38.0	37.0	38.0	33.0	38.0
100-101	35.63818274313928	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	16.0
18	41.0
19	69.0
20	191.0
21	796.0
22	3231.0
23	9316.0
24	21188.0
25	39569.0
26	65226.0
27	98909.0
28	138383.0
29	184937.0
30	237317.0
31	301543.0
32	385328.0
33	509554.0
34	716969.0
35	1158045.0
36	2715389.0
37	7177211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.698757973147146	18.365595747916423	13.706325746668895	21.229320532267536
2	14.859645218429485	20.314426214952903	40.59465260659399	24.231275960023627
3	20.351083798554704	25.348292978948894	29.317708909304685	24.98291431319172
4	13.222580253514638	17.976493742856416	39.11225338181905	29.688672621809896
5	14.051332535592515	37.96111198857917	34.22315378398591	13.764401691842409
6	30.046531958452483	37.48817955437638	18.667792274618115	13.797496212553028
7	26.736455608111036	33.030560659611	22.509491193830193	17.723492538447772
8	23.982889480368303	37.187850322042884	20.700767240013228	18.12849295757558
9	25.181619807386767	18.041602010690948	21.550466100651235	35.22631208127105
10-11	24.249564700607856	27.094169801801954	28.2413969948984	20.414868502691792
12-13	25.050088173349437	24.55268672780203	28.679160246479952	21.71806485236858
14-15	22.248082190596406	26.013924494026803	26.537061179465955	25.200932135910836
16-17	21.598209257435155	29.14391673639958	27.299578463745682	21.958295542419588
18-19	21.522503912417644	28.214222113139293	29.182145410789868	21.081128563653195
20-21	22.392714201405266	27.23397790273075	29.27068575242385	21.10262214344013
22-23	22.14163236675667	27.148143406736924	29.104566800478658	21.605657426027744
24-25	21.876599420156907	27.284178302222976	29.197414737158667	21.641807540461443
26-27	21.74643866904682	27.3971738451123	29.12601845757704	21.73036902826384
28-29	21.71075393977531	27.373271631209338	29.229199563159558	21.6867748658558
30-31	21.971442265346774	27.280877448343894	29.128119486090277	21.619560800219055
32-33	21.778031138399918	27.433836354870373	29.029997328475698	21.758135178254012
34-35	21.95835496260512	27.449253256516936	29.012376997557975	21.580014783319964
36-37	21.938176869209624	27.349142928376548	28.942523042170524	21.770157160243304
38-39	21.73695223515912	27.548436821323936	29.023703931980798	21.69090701153615
40-41	21.991881838846915	27.35584799427977	28.896094241678504	21.756175925194807
42-43	21.870609209538408	27.46721673821137	28.779148425736494	21.88302562651373
44-45	21.9463295051522	27.576603106315318	28.60542584623169	21.871641542300793
46-47	22.018286089057256	27.582814829417345	28.348442213627088	22.050456867898312
48-49	21.95549455429207	27.668432044862527	28.36060811309671	22.015465287748697
50-51	21.977021744968862	27.81847693565475	28.218133406234468	21.986367913141915
52-53	22.170635429226856	27.852921087746594	27.892337995673156	22.08410548735339
54-55	22.182061718307697	27.782161620145153	27.907973053863543	22.127803607683607
56-57	22.095971787094104	27.853426002933755	27.943432701893613	22.10716950807853
58-59	22.053003417085552	27.844730459765287	28.006043967103047	22.096222156046114
60-61	22.064971594073743	27.705202979041676	28.112207387132194	22.117618039752383
62-63	22.168634560322452	27.76507826343751	28.106568344329226	21.959718831910816
64-65	21.963984903107008	27.722177442389956	28.066086554200993	22.247751100302047
66-67	22.00855206919983	27.821327043031612	27.892634980272813	22.277485907495745
68-69	21.96592471895602	27.964203879468354	27.957416383611537	22.112455017964088
70-71	21.949491491890072	27.764162005704495	28.026253430789627	22.260093071615806
72-73	22.014083866861515	27.795892825310077	27.775424442429603	22.414598865398805
74-75	21.907253451223415	27.910571297682896	27.756501979364522	22.425673271729167
76-77	22.10394033222835	27.67243366193196	27.83958991135916	22.384036094480532
78-79	22.049694812697833	27.68949467572704	27.769043230400868	22.49176728117426
80-81	22.21293327961373	27.7554858522292	27.58077324590903	22.45080762224804
82-83	22.284985352781053	27.90868498235109	27.673449040487615	22.132880624380245
84-85	22.209089593644755	27.728710053293014	27.603200002253907	22.45900035080832
86-87	22.060558395595752	27.967335085512467	27.645291763413525	22.326814755478257
88-89	22.353543789351278	27.780376487555753	27.567095382008993	22.298984341083976
90-91	22.3839996330399	28.008116453990567	27.53888254122046	22.069001371749074
92-93	22.213861660165986	27.931638391155168	27.570387877943638	22.284112070735212
94-95	22.25478267591596	27.864903199205454	27.599176980997964	22.28113714388062
96-97	22.324559688530783	28.0383294077117	27.62242355586774	22.01468734788978
98-99	22.585433636581094	28.463523745727915	27.771460956921434	21.179581660769557
100-101	23.0058949088706	31.076390155697307	25.192918369324747	20.724796566107347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3733.0
1	3042.0
2	2698.5
3	3560.5
4	4921.5
5	7248.5
6	9184.5
7	9874.5
8	10143.0
9	10409.5
10	10525.5
11	11609.0
12	14281.0
13	20434.0
14	30856.0
15	44836.0
16	58125.5
17	66294.0
18	70512.5
19	67332.5
20	61341.5
21	56938.5
22	55310.5
23	59991.5
24	70605.5
25	88377.0
26	111574.0
27	136764.0
28	164481.0
29	195654.0
30	226803.5
31	258581.0
32	291926.5
33	325571.0
34	358400.5
35	387305.5
36	418630.0
37	448863.0
38	471358.5
39	490402.0
40	508003.0
41	524456.5
42	535826.0
43	543729.0
44	551956.0
45	560649.5
46	566529.5
47	567188.0
48	564055.5
49	557903.5
50	548620.5
51	534375.5
52	510465.5
53	479353.0
54	449803.5
55	417851.0
56	382367.5
57	345220.0
58	307580.5
59	268272.0
60	224091.0
61	181656.0
62	144412.5
63	112075.0
64	84465.5
65	62970.5
66	47266.5
67	35778.0
68	26902.0
69	19891.5
70	14446.0
71	9812.0
72	6448.0
73	4155.5
74	2634.5
75	1658.0
76	1061.0
77	657.5
78	417.5
79	266.0
80	176.0
81	109.5
82	71.5
83	52.5
84	42.0
85	31.0
86	18.5
87	11.5
88	6.5
89	3.0
90	2.0
91	2.0
92	1.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0017946369997912555
4	0.0
5	0.03785448894296535
6	0.0
7	0.0015112732629821099
8	0.0
9	0.0
10-11	3.6328684206300716E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.997162741210603E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	5.269520027095189E-4
48-49	1.1464474231186627E-4
50-51	5.350537170836108E-4
52-53	0.0010761251723936988
54-55	0.0026928995469518956
56-57	0.0024236222592707683
58-59	0.006239940077558295
60-61	0.005434227822084813
62-63	0.0062281256624146675
64-65	0.0034638405926025687
66-67	0.0058066836597276865
68-69	0.006368514327782591
70-71	0.0029428075899270885
72-73	0.002429100625493411
74-75	0.0033280019387399405
76-77	0.0018626314009020481
78-79	0.0
80-81	4.538081440063765E-4
82-83	1.4397638787238892E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	9.065219084135477E-6
92-93	5.956884073079053E-4
94-95	6.021228071150536E-5
96-97	0.0
98-99	1.4529466144794074E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1469.0
20-21	5222.0
22-23	2845.0
24-25	3137.0
26-27	5242.0
28-29	9854.0
30-31	24303.0
32-33	32272.0
34-35	45479.0
36-37	69050.0
38-39	76549.0
40-41	77709.0
42-43	87819.0
44-45	105746.0
46-47	107483.0
48-49	97643.0
50-51	122632.0
52-53	75564.0
54-55	83424.0
56-57	85748.0
58-59	51448.0
60-61	94657.0
62-63	89717.0
64-65	95276.0
66-67	102052.0
68-69	104513.0
70-71	102566.0
72-73	95989.0
74-75	97851.0
76-77	104461.0
78-79	109038.0
80-81	110048.0
82-83	102986.0
84-85	101735.0
86-87	107278.0
88-89	113499.0
90-91	120799.0
92-93	114795.0
94-95	123422.0
96-97	200250.0
98-99	1200045.0
100-101	9301614.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.81751161026335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.65350510574778	71.71591671669798
2	8.320787200808903	13.615722068174263
3	2.188639604943578	5.372071388644601
4	0.8230578587688343	2.6936218366295033
5	0.3815425489373095	1.5608430963743893
6	0.20656932684208948	1.0140592978336165
7	0.12430739126240851	0.711936499949756
8	0.07866832338243017	0.5149157169361546
9	0.04949070168914565	0.3644285454046578
>10	0.1692568645253209	2.082331110565861
>50	0.0031356266230179596	0.17149849741877915
>100	9.737561582232814E-4	0.11824186627440786
>500	3.285853287891811E-5	0.01410056487148263
>1k	3.283177804507822E-5	0.050312794224545106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4531473682520286E-5	0.0	7.265736841260143E-6	7.265736841260143E-6
3	0.0	1.4531473682520286E-5	0.0	1.4531473682520286E-5	7.265736841260143E-6
4	0.0	6.539163157134128E-5	0.0	1.4531473682520286E-5	7.265736841260143E-6
5	0.0	6.539163157134128E-5	0.0	1.4531473682520286E-5	7.265736841260143E-6
6	0.0	7.265736841260143E-5	0.0	1.4531473682520286E-5	7.265736841260143E-6
7	0.0	7.265736841260143E-5	0.0	1.4531473682520286E-5	7.265736841260143E-6
8	0.0	9.445457893638187E-5	0.0	1.4531473682520286E-5	1.4531473682520286E-5
9	0.0	2.03440631555284E-4	0.0	1.4531473682520286E-5	1.4531473682520286E-5
10-11	7.265736841260143E-6	2.03440631555284E-4	0.0	2.179721052378043E-5	1.4531473682520286E-5
12-13	7.265736841260143E-6	2.1070636839654416E-4	0.0	2.179721052378043E-5	1.8164342103150357E-5
14-15	1.4531473682520286E-5	2.760979999678854E-4	0.0	2.179721052378043E-5	3.996155262693079E-5
16-17	1.4531473682520286E-5	2.8336373680914556E-4	0.0	2.179721052378043E-5	4.722728946819093E-5
18-19	2.179721052378043E-5	2.906294736504057E-4	0.0	2.179721052378043E-5	5.449302630945107E-5
20-21	3.996155262693079E-5	2.978952104916659E-4	0.0	3.269581578567064E-5	5.8125894730081145E-5
22-23	5.0860157888821E-5	3.305910262773365E-4	0.0	6.175876315071122E-5	6.902449999197136E-5
24-25	5.8125894730081145E-5	3.6691971048363726E-4	0.0	7.265736841260143E-5	9.445457893638187E-5
26-27	7.992310525386158E-5	3.9234978942804773E-4	0.0	1.0535318419827208E-4	9.445457893638187E-5
28-29	7.992310525386158E-5	3.9961552626930784E-4	0.0	3.56021105221747E-4	9.808744735701193E-5
30-31	7.992310525386158E-5	4.4320994731686874E-4	0.0	0.001271503947220525	1.0535318419827207E-4
32-33	8.718884209512172E-5	4.4320994731686874E-4	0.0	0.0035638439206381	1.5258047366646302E-4
34-35	9.08217105157518E-5	5.55828868356401E-4	0.0	0.006775299604475083	1.5984621050772315E-4
36-37	1.01720315777642E-4	6.684477893959331E-4	0.0	0.011516192893397326	1.6711194734898328E-4
38-39	1.1625178946016228E-4	6.866121314990835E-4	0.0	0.020936220708091105	1.6711194734898328E-4
40-41	1.3441613156331265E-4	7.338394209672745E-4	0.0	0.05299991738857211	1.6711194734898328E-4
42-43	1.3804899998394273E-4	7.447380262291647E-4	0.0	0.062419945203265895	1.7437768419024344E-4
44-45	1.416818684045728E-4	8.06496789379876E-4	0.0	0.07572714222803384	1.8164342103150357E-4
46-47	1.5258047366646302E-4	8.57356947268697E-4	0.0	0.09067276291050597	1.8890915787276373E-4
48-49	1.5984621050772315E-4	8.60989815689327E-4	0.0	0.10448492864574149	2.03440631555284E-4
50-51	1.7437768419024344E-4	9.627101314669689E-4	0.0	0.11838428322307215	2.03440631555284E-4
52-53	1.780105526108735E-4	9.663429998875991E-4	0.0	0.1421759385097785	2.179721052378043E-4
54-55	1.8164342103150357E-4	9.736087367288593E-4	0.0	0.2024234283975076	2.3250357892032458E-4
56-57	1.8164342103150357E-4	9.954059472526396E-4	0.0	0.2694207878107674	2.3250357892032458E-4
58-59	1.8164342103150357E-4	0.0010099374209351598	0.0	0.3348887096189419	2.3613644734095466E-4
60-61	1.8164342103150357E-4	0.0010390003683002004	0.0	0.6675105093434106	2.397693157615847E-4
62-63	1.8527628945213365E-4	0.001057164710403351	0.0	0.6737299800795293	2.4703505260284487E-4
64-65	1.8890915787276373E-4	0.001169783631442883	0.0	0.6794917093946486	2.4703505260284487E-4
66-67	1.8890915787276373E-4	0.0011770493682841433	0.0	0.6846067881308957	2.54300789444105E-4
68-69	1.9617489471402387E-4	0.0011952137103872936	0.0	0.6900306606828964	2.6156652628536514E-4
70-71	1.9617489471402387E-4	0.0012061123156491837	0.0	0.6952183967875561	2.6156652628536514E-4
72-73	2.1070636839654416E-4	0.0012097451840698138	0.0	0.6990147442871146	2.7609799996788546E-4
74-75	2.1070636839654416E-4	0.0012315423945935943	0.0	0.7022261999709516	2.7609799996788546E-4
76-77	2.1070636839654416E-4	0.0012824025524824153	0.0	0.7050126100495748	2.797308683885155E-4
78-79	2.1433923681717424E-4	0.001322364105109346	0.0	0.7074684291019208	2.8336373680914556E-4
80-81	2.179721052378043E-4	0.0013259969735299761	0.0	0.7086200483912606	2.8336373680914556E-4
82-83	2.179721052378043E-4	0.0013296298419506062	0.0	0.7090487268648948	2.8336373680914556E-4
84-85	2.3250357892032458E-4	0.0013441613156331264	0.0	0.7094083808385372	2.8336373680914556E-4
86-87	2.397693157615847E-4	0.0013514270524743867	0.0	0.7095209997595768	2.8699660522977567E-4
88-89	2.397693157615847E-4	0.0014022872103632076	0.0	0.7095645941806243	2.906294736504057E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20315	0.0	50.092716	1
GTATCAA	35710	0.0	36.138584	1
TCAACGC	41380	0.0	31.112144	4
ATCAACG	41370	0.0	30.984255	3
CAACGCA	42310	0.0	30.428278	5
GTGGTAT	10155	0.0	30.037357	1
TGGTATC	9625	0.0	30.02102	2
TATCAAC	42410	0.0	30.01953	2
AACGCAG	43805	0.0	29.46826	6
ACGCAGA	50985	0.0	25.292812	7
CGCAGAG	52565	0.0	24.589775	8
CGAGACC	10945	0.0	22.207806	88-89
GACCGAG	11525	0.0	21.857317	90-91
AGACCGA	11290	0.0	21.765648	90-91
GAGACCG	11530	0.0	21.63391	88-89
ACCGAGG	11885	0.0	21.453068	92-93
GCAGAGT	60710	0.0	21.240675	9
GAGTACT	34935	0.0	20.836828	12-13
ATCTCCG	11530	0.0	20.271166	74-75
ACGAGAC	12270	0.0	20.225683	86-87
>>END_MODULE