FastQCFastQC Report
Fri 27 May 2016
SRR2139490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12954470
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177350.050.859511
GTATCAA316500.038.156151
TATCTCG137750.035.86034894-95
TCAACGC362150.032.9573554
ATCAACG362750.032.8791053
CAACGCA371550.032.1590655
TATCAAC377650.031.4421312
AACGCAG385550.031.0832886
CCGTCTT69750.030.11512692-93
TGCCGTC70750.028.70332590-91
TATGCCG71150.028.22305188-89
GCCGTCT71200.027.54647390-91
TCTCGTA65350.027.34613882-83
ACGCAGA447050.026.7875397
ATGCCGT72650.026.62081188-89
CGCAGAG460350.026.0896138
CTCGTAT80050.024.37785384-85
GTGGTAT85950.024.304431
CGTATGC86700.023.02900586-87
TCGTATG82800.022.95225384-85
Produced by FastQC (version 0.11.5)