##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12954470
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.682481645331688	30.0	18.0	33.0	18.0	33.0
2	27.811749149135395	29.0	25.0	32.0	18.0	33.0
3	27.608325311649185	29.0	25.0	31.0	18.0	33.0
4	30.81716395962166	33.0	30.0	33.0	27.0	33.0
5	31.70607944593642	33.0	32.0	33.0	30.0	33.0
6	35.79975444769257	38.0	36.0	38.0	31.0	38.0
7	36.05848182133271	38.0	37.0	38.0	33.0	38.0
8	36.49719672051423	38.0	37.0	38.0	34.0	38.0
9	36.7350871938412	38.0	38.0	38.0	34.0	38.0
10-11	36.79872773644927	38.0	38.0	38.0	34.5	38.0
12-13	36.85603235794286	38.0	38.0	38.0	35.0	38.0
14-15	36.84853946166844	38.0	38.0	38.0	35.0	38.0
16-17	36.86185687256986	38.0	38.0	38.0	35.0	38.0
18-19	36.870838289794946	38.0	38.0	38.0	35.0	38.0
20-21	36.884125875859596	38.0	38.0	38.0	35.0	38.0
22-23	36.89330004641688	38.0	38.0	38.0	35.0	38.0
24-25	36.88648459229409	38.0	38.0	38.0	35.0	38.0
26-27	36.83882851186958	38.0	38.0	38.0	35.0	38.0
28-29	36.830480937791194	38.0	38.0	38.0	35.0	38.0
30-31	36.81779127417766	38.0	38.0	38.0	35.0	38.0
32-33	36.80219165513705	38.0	38.0	38.0	35.0	38.0
34-35	36.77581630364742	38.0	38.0	38.0	35.0	38.0
36-37	36.74880680533691	38.0	38.0	38.0	35.0	38.0
38-39	36.736144398890445	38.0	38.0	38.0	35.0	38.0
40-41	36.729657378896206	38.0	38.0	38.0	35.0	38.0
42-43	36.68165682837089	38.0	38.0	38.0	34.0	38.0
44-45	36.63633233669605	38.0	38.0	38.0	34.0	38.0
46-47	36.595693328607766	38.0	38.0	38.0	34.0	38.0
48-49	36.55150344022266	38.0	38.0	38.0	34.0	38.0
50-51	36.53060343043002	38.0	38.0	38.0	34.0	38.0
52-53	36.50371625748491	38.0	38.0	38.0	34.0	38.0
54-55	36.43457166658646	38.0	38.0	38.0	34.0	38.0
56-57	36.44087036866502	38.0	38.0	38.0	34.0	38.0
58-59	36.435642967337124	38.0	38.0	38.0	34.0	38.0
60-61	36.39660414387909	38.0	38.0	38.0	34.0	38.0
62-63	36.403030365850086	38.0	38.0	38.0	34.0	38.0
64-65	36.42931806972454	38.0	38.0	38.0	34.0	38.0
66-67	36.41092794316748	38.0	38.0	38.0	34.0	38.0
68-69	36.392664459907174	38.0	38.0	38.0	34.0	38.0
70-71	36.389788127217436	38.0	38.0	38.0	34.0	38.0
72-73	36.38007130803908	38.0	38.0	38.0	34.0	38.0
74-75	36.368111903005214	38.0	38.0	38.0	34.0	38.0
76-77	36.324231685347456	38.0	37.5	38.0	33.0	38.0
78-79	36.313832889412254	38.0	37.0	38.0	33.0	38.0
80-81	36.29513910153	38.0	37.0	38.0	33.0	38.0
82-83	36.25352596577031	38.0	37.0	38.0	33.0	38.0
84-85	36.21761455478101	38.0	37.0	38.0	33.0	38.0
86-87	36.23980018716398	38.0	37.0	38.0	33.0	38.0
88-89	36.235849694828346	38.0	37.0	38.0	33.0	38.0
90-91	36.22614305448536	38.0	37.0	38.0	33.0	38.0
92-93	36.22048332813701	38.0	37.0	38.0	33.0	38.0
94-95	36.197082628445926	38.0	37.0	38.0	33.0	38.0
96-97	36.13691784843319	38.0	37.0	38.0	33.0	38.0
98-99	36.16195731034004	38.0	37.0	38.0	33.0	38.0
100-101	35.65686297029201	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	21.0
19	53.0
20	157.0
21	672.0
22	2675.0
23	8224.0
24	18659.0
25	35094.0
26	57681.0
27	88086.0
28	125034.0
29	167011.0
30	215533.0
31	273614.0
32	350229.0
33	461817.0
34	650240.0
35	1046249.0
36	2495259.0
37	6958162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.40531029057924	18.520611032330926	13.648593883038055	21.425484794051783
2	14.678647601947436	20.545024227158656	40.68159484718402	24.094733323709885
3	19.953896094221673	25.768867388897377	29.45761986231974	24.819616654561212
4	12.918946124387954	18.230850046354654	39.53656151120038	29.31364231805701
5	13.8123032137874	38.3021379017852	34.41820782724545	13.467351057181945
6	29.627881341343954	37.89820038951806	18.996917666257286	13.477000602880704
7	26.621049860702517	33.167730535598636	22.789994665859727	17.421224937839124
8	24.018142000406037	37.099580299309814	21.038984999000345	17.843292701283804
9	25.417867346174717	17.813364807668705	21.625987014520856	35.142780831635726
10-11	24.0122637205536	27.173006691898628	28.64276191924486	20.17196766830291
12-13	24.898791691207744	24.64551232122966	28.958502354785647	21.49719363277695
14-15	22.13518747854505	26.036254361332755	26.727560195635963	25.100997964486233
16-17	21.49744451143119	29.198168663017476	27.462061357971418	21.84232546757992
18-19	21.498598553240697	28.31451614770809	29.228316557913985	20.958568741137228
20-21	22.355517021760868	27.43422244322159	29.22814230086252	20.982118234155024
22-23	22.09419774417876	27.29264473447944	29.080606863559915	21.532550657781886
24-25	21.78768154837903	27.432907995414833	29.187708222472754	21.591702233733386
26-27	21.681908799230456	27.586559949990168	29.145301930275476	21.5862293205039
28-29	21.659421804671762	27.508834774472508	29.239831508346942	21.591911912508785
30-31	21.867845872288147	27.418969683467026	29.157590373442083	21.55559407080274
32-33	21.66639462736909	27.6008476783237	29.071861939572425	21.660895754734792
34-35	21.85106777873893	27.584693968620705	29.055970867867227	21.508267384773138
36-37	21.826876978639124	27.484412935787628	29.026589909770838	21.662120175802407
38-39	21.6780020414523	27.64755226438636	29.09892357117627	21.575522122985067
40-41	21.884773038398247	27.43713192707932	29.011596016050216	21.666499018472212
42-43	21.871188641915246	27.468032667945025	28.92983122636896	21.730947463770768
44-45	21.80257070673439	27.540483156134055	28.804621408311874	21.85232472881968
46-47	21.84848924985295	27.55828864303317	28.565218682217257	22.02800342489662
48-49	21.787264167290814	27.747248536671638	28.59377362542895	21.8717136706086
50-51	21.81019522723559	27.903309215938364	28.42490008744436	21.861595469381683
52-53	21.890750250431772	27.82307418111288	28.164933099321328	22.12124246913402
54-55	21.925126131380498	27.578669158458247	28.279391311515628	22.21681339864563
56-57	21.8312766892644	27.364476630844532	28.610272476696924	22.19397420319415
58-59	21.68662122845904	27.189021628557846	28.75775413946129	22.366603003521824
60-61	21.78128295732402	27.025094082150318	28.949664086579503	22.243958873946156
62-63	21.561371460556064	27.481567853604293	28.98024048057341	21.97682020526623
64-65	21.263895176535193	27.60218912363464	28.80699607880461	22.326919621025556
66-67	21.316986548654135	28.125316350784864	28.357081381174954	22.20061571938605
68-69	21.371836912431156	28.33096074167521	28.06776356147577	22.229438784417866
70-71	21.39302966888385	27.985956487590197	28.037201678481317	22.583812165044638
72-73	21.320285458905573	27.695960929998485	27.961254210785004	23.022499400310938
74-75	21.65485334031661	27.462129745138032	28.000694551258448	22.882322363286907
76-77	22.024203248168025	27.325402209724174	27.893999319244905	22.756395222862892
78-79	22.159210742051478	27.19525401572277	27.302375454853443	23.343159787372308
80-81	22.03646520956081	27.646209996108517	27.24469973431741	23.07262506001326
82-83	22.085346760377885	27.74027826068859	27.295698186484834	22.87867679244869
84-85	22.092016239890146	27.75052931639606	27.237417368958393	22.9200370747554
86-87	22.335882854233684	28.026231812413304	27.171659644954698	22.46622568839831
88-89	22.49689463272816	27.767408049568488	27.459510273158187	22.27618704454516
90-91	22.269571623278427	27.825412019120737	27.591320384446817	22.313695973154015
92-93	22.098783348284122	27.390396562329084	27.529587165449808	22.981232923936982
94-95	21.926304423011782	27.452421227218892	28.054463542699036	22.566810807070286
96-97	21.699618545313207	27.630064700774636	28.232487024311265	22.43782972960089
98-99	21.975873366855026	27.823098217804908	28.553297485490347	21.647730929849722
100-101	22.735099839766733	30.636786771028923	25.879824425478322	20.748288963726026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1743.0
1	1692.0
2	2489.0
3	3676.5
4	4750.0
5	6960.0
6	9217.5
7	11642.0
8	15125.0
9	17487.5
10	17104.0
11	15879.5
12	16664.0
13	20371.0
14	27961.5
15	37112.5
16	44958.5
17	50929.5
18	52407.5
19	49290.0
20	44899.5
21	41401.5
22	40494.0
23	45134.5
24	55569.5
25	71184.5
26	91756.5
27	114723.5
28	142524.5
29	174798.5
30	205182.5
31	235748.0
32	268723.5
33	302629.0
34	334514.0
35	362785.5
36	394907.5
37	423680.5
38	447100.0
39	469313.5
40	488148.0
41	504833.0
42	515789.5
43	525958.0
44	534881.0
45	540813.5
46	542512.5
47	540119.5
48	534557.0
49	525672.5
50	515690.5
51	499526.5
52	475207.5
53	445835.0
54	413262.5
55	380710.5
56	345494.5
57	306840.0
58	270083.5
59	231611.5
60	190965.0
61	152536.5
62	118835.5
63	90394.0
64	66612.5
65	48692.5
66	35996.0
67	26943.5
68	20189.5
69	14690.5
70	10499.5
71	7315.5
72	4898.0
73	3189.5
74	2039.5
75	1251.0
76	790.5
77	476.5
78	289.5
79	191.0
80	130.0
81	88.5
82	63.0
83	42.0
84	30.0
85	23.0
86	17.5
87	14.5
88	7.5
89	3.0
90	2.5
91	3.0
92	2.5
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001952993831472843
4	0.0
5	0.03966198539963426
6	0.0
7	0.0013894817773324574
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.719343207402541E-6
16-17	0.0
18-19	0.0
20-21	3.8597250849052674E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	5.215958747383494E-4
48-49	1.0918613704865989E-4
50-51	4.8064025844434015E-4
52-53	0.0010031091059665217
54-55	0.002781720369096193
56-57	0.002336412109648558
58-59	0.00613120231606065
60-61	0.005323453661062406
62-63	0.006341725909612055
64-65	0.003577307962636709
66-67	0.005619021266669658
68-69	0.0062367927210642635
70-71	0.003019622923195441
72-73	0.0024410378117621125
74-75	0.0035162055027352546
76-77	0.002184733888422783
78-79	0.0
80-81	4.883929604382884E-4
82-83	2.081193606157003E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.8248615500043576E-5
92-93	6.853819407474938E-4
94-95	8.661987172463196E-5
96-97	0.0
98-99	5.03641250696347E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	105.0
20-21	439.0
22-23	1033.0
24-25	1303.0
26-27	3089.0
28-29	7534.0
30-31	22524.0
32-33	29576.0
34-35	40795.0
36-37	62392.0
38-39	42876.0
40-41	73978.0
42-43	83072.0
44-45	98392.0
46-47	99814.0
48-49	91234.0
50-51	82306.0
52-53	7660.0
54-55	6597.0
56-57	6405.0
58-59	6704.0
60-61	93145.0
62-63	91686.0
64-65	96980.0
66-67	102298.0
68-69	103129.0
70-71	101955.0
72-73	97541.0
74-75	99533.0
76-77	105288.0
78-79	108585.0
80-81	109091.0
82-83	103805.0
84-85	104286.0
86-87	108056.0
88-89	112585.0
90-91	115270.0
92-93	113656.0
94-95	123710.0
96-97	202674.0
98-99	1218633.0
100-101	8874736.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.03494448372022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.68448677678515	71.9319200481707
2	8.277269189831271	13.580506369292323
3	2.2334509650142134	5.49663077766157
4	0.8144579865520274	2.6725606284447245
5	0.3776646841439364	1.5490850698604777
6	0.21042288144135887	1.035721763828779
7	0.12539117403549677	0.7200520600525334
8	0.0739633887979583	0.4854065995094658
9	0.04531345356740021	0.3345557982990579
>10	0.15428751865117485	1.9146851911811722
>50	0.0026501946649808918	0.14301477177865984
>100	5.749808448705906E-4	0.08167632922525683
>500	5.010627591493345E-5	0.03152588622426191
>1k	1.669939431800659E-5	0.022658706470957062
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5438686414805082E-5	0.0	0.0	0.0
3	0.0	1.5438686414805082E-5	0.0	7.719343207402541E-6	0.0
4	0.0	3.8596716037012704E-5	0.0	1.5438686414805082E-5	0.0
5	0.0	3.8596716037012704E-5	0.0	2.3158029622207625E-5	0.0
6	0.0	3.8596716037012704E-5	0.0	2.3158029622207625E-5	0.0
7	0.0	3.8596716037012704E-5	0.0	2.3158029622207625E-5	0.0
8	0.0	5.403540245181779E-5	0.0	2.3158029622207625E-5	0.0
9	0.0	9.26321184888305E-5	0.0	3.8596716037012704E-5	0.0
10-11	0.0	9.26321184888305E-5	0.0	4.631605924441525E-5	0.0
12-13	0.0	9.26321184888305E-5	0.0	5.017573084811652E-5	0.0
14-15	0.0	1.3894817773324575E-4	0.0	7.333376047032413E-5	0.0
16-17	0.0	1.466675209406483E-4	0.0	8.491277528142795E-5	0.0
18-19	0.0	1.6210620735545336E-4	0.0	8.491277528142795E-5	0.0
20-21	0.0	1.85264236977661E-4	0.0	8.877244688512923E-5	0.0
22-23	0.0	1.9298358018506354E-4	0.0	1.1579014811103811E-4	0.0
24-25	0.0	2.0456259499616734E-4	0.0	1.6596587895915463E-4	0.0
26-27	0.0	2.2000128141097243E-4	0.0	2.740366838627902E-4	0.0
28-29	0.0	2.238609530146737E-4	0.0	7.680746491365529E-4	0.0
30-31	0.0	2.7403668386279025E-4	0.0	0.0026322960337242665	0.0
32-33	0.0	2.778963554664915E-4	0.0	0.0066810915460068995	0.0
34-35	0.0	3.3579142952201057E-4	0.0	0.011984280329492445	0.0
36-37	0.0	3.9368650357752964E-4	0.0	0.019371691778976678	0.0
38-39	0.0	4.091251899923347E-4	0.0	0.04408130938587221	0.0
40-41	0.0	5.017573084811652E-4	0.0	0.31260638219857706	0.0
42-43	0.0	5.094766516885677E-4	0.0	0.3378061780991426	0.0
44-45	0.0	5.55792710932983E-4	0.0	0.3795987022240199	0.0
46-47	0.0	5.943894269699957E-4	0.0	0.42683722298171983	0.0
48-49	0.0	5.943894269699957E-4	0.0	0.47253959444114657	0.0
50-51	0.0	6.715828590440211E-4	0.0	0.5166093248122077	0.0
52-53	0.0	6.870215454588262E-4	0.0	0.6972535348802382	0.0
54-55	0.0	6.9860056026993E-4	0.0	1.322018577371363	0.0
56-57	0.0	7.178989182884363E-4	0.0	2.000668495121761	0.0
58-59	0.0	7.217585898921376E-4	0.0	2.6946644671684754	0.0
60-61	0.0	7.256182614958389E-4	0.0	3.2146664433203367	0.0
62-63	0.0	7.371972763069427E-4	0.0	3.2231885982213093	0.0
64-65	0.0	7.873730071550593E-4	0.0	3.2290668780737457	0.0
66-67	0.0	7.873730071550593E-4	0.0	3.2344626989757206	0.0
68-69	0.0	8.221100515883706E-4	0.0	3.239831502176469	0.0
70-71	0.0	8.375487380031757E-4	0.0	3.2450999539155214	0.0
72-73	0.0	8.41408409606877E-4	0.0	3.249168047785822	0.0
74-75	0.0	8.529874244179808E-4	0.0	3.252302101128028	0.0
76-77	0.0	8.838647972475909E-4	0.0	3.2554940495442883	0.0
78-79	0.0	9.031631552660973E-4	0.0	3.258126345578013	0.0
80-81	0.0	9.031631552660973E-4	0.0	3.259311264760349	0.0
82-83	0.0	9.070228268697986E-4	0.0	3.2597705656811895	0.0
84-85	0.0	9.301808564920063E-4	0.0	3.2602028489008044	0.0
86-87	0.0	9.456195429068113E-4	0.0	3.260318639048915	0.0
88-89	0.0	9.726372441327202E-4	0.0	3.260372674451367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17735	0.0	50.85951	1
GTATCAA	31650	0.0	38.15615	1
TATCTCG	13775	0.0	35.860348	94-95
TCAACGC	36215	0.0	32.957355	4
ATCAACG	36275	0.0	32.879105	3
CAACGCA	37155	0.0	32.159065	5
TATCAAC	37765	0.0	31.442131	2
AACGCAG	38555	0.0	31.083288	6
CCGTCTT	6975	0.0	30.115126	92-93
TGCCGTC	7075	0.0	28.703325	90-91
TATGCCG	7115	0.0	28.223051	88-89
GCCGTCT	7120	0.0	27.546473	90-91
TCTCGTA	6535	0.0	27.346138	82-83
ACGCAGA	44705	0.0	26.787539	7
ATGCCGT	7265	0.0	26.620811	88-89
CGCAGAG	46035	0.0	26.089613	8
CTCGTAT	8005	0.0	24.377853	84-85
GTGGTAT	8595	0.0	24.30443	1
CGTATGC	8670	0.0	23.029005	86-87
TCGTATG	8280	0.0	22.952253	84-85
>>END_MODULE