FastQCFastQC Report
Fri 27 May 2016
SRR2139489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5335658
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71900.049.1252671
GTATCAA134500.037.192631
CTTATAC72850.034.6236041
TCAACGC164100.030.120074
ATCAACG164650.030.0194533
TATCAAC169050.029.5163212
CAACGCA168050.029.4120985
AACGCAG171900.028.8762426
TATACAC93350.028.0127033
TTATACA96100.026.7745022
ACGCAGA196600.025.2053767
CGCAGAG201850.024.5497998
TACACAT119050.022.2848075
TGGTATC34600.021.85772
ACACATC117500.021.4282676
GTACATG166250.021.4236491
GCAGAGT232750.021.2905569
TACATGG170050.020.9200612
ACATCTC122300.020.6563428
GTGGTAT36250.020.513141
Produced by FastQC (version 0.11.5)