##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139489_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5335658
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.160598186765345	30.0	18.0	32.0	18.0	33.0
2	30.28298046838834	32.0	29.0	33.0	25.0	33.0
3	31.950687244197436	33.0	31.0	33.0	29.0	33.0
4	32.079177488512194	33.0	32.0	33.0	31.0	33.0
5	32.90003800843307	33.0	33.0	34.0	32.0	34.0
6	36.825524424541456	38.0	37.0	38.0	35.0	38.0
7	37.005326615761355	38.0	38.0	38.0	35.0	38.0
8	36.91519752577845	38.0	38.0	38.0	35.0	38.0
9	37.24082371846171	38.0	38.0	38.0	36.0	38.0
10-11	37.4189917157359	38.0	38.0	38.0	37.0	38.0
12-13	37.48181320841778	38.0	38.0	38.0	37.0	38.0
14-15	37.434581639228	38.0	38.0	38.0	37.0	38.0
16-17	37.46805220649449	38.0	38.0	38.0	37.0	38.0
18-19	37.487740499859626	38.0	38.0	38.0	37.0	38.0
20-21	37.5048034036505	38.0	38.0	38.0	37.0	38.0
22-23	37.519167010313595	38.0	38.0	38.0	37.5	38.0
24-25	37.528903757099386	38.0	38.0	38.0	38.0	38.0
26-27	37.502445028573455	38.0	38.0	38.0	37.5	38.0
28-29	37.477539989001144	38.0	38.0	38.0	37.0	38.0
30-31	37.48047142816644	38.0	38.0	38.0	37.0	38.0
32-33	37.46057664420938	38.0	38.0	38.0	37.0	38.0
34-35	37.35883282854482	38.0	38.0	38.0	37.0	38.0
36-37	37.393735000104925	38.0	38.0	38.0	37.0	38.0
38-39	37.37879424871588	38.0	38.0	38.0	37.0	38.0
40-41	37.40815781553172	38.0	38.0	38.0	37.0	38.0
42-43	37.378720235825156	38.0	38.0	38.0	37.0	38.0
44-45	37.34250099405931	38.0	38.0	38.0	37.0	38.0
46-47	37.307389749709486	38.0	38.0	38.0	37.0	38.0
48-49	37.244564870368215	38.0	38.0	38.0	37.0	38.0
50-51	37.21237564937056	38.0	38.0	38.0	37.0	38.0
52-53	37.18748582072719	38.0	38.0	38.0	37.0	38.0
54-55	37.15469452131863	38.0	38.0	38.0	36.5	38.0
56-57	37.10880928253425	38.0	38.0	38.0	36.0	38.0
58-59	37.09534504577641	38.0	38.0	38.0	36.0	38.0
60-61	37.08945467904138	38.0	38.0	38.0	36.0	38.0
62-63	37.09514350879874	38.0	38.0	38.0	36.0	38.0
64-65	37.10070346109116	38.0	38.0	38.0	36.0	38.0
66-67	37.094782381025155	38.0	38.0	38.0	36.0	38.0
68-69	37.085204544816676	38.0	38.0	38.0	36.0	38.0
70-71	37.08279987067691	38.0	38.0	38.0	36.0	38.0
72-73	37.07265159229501	38.0	38.0	38.0	36.0	38.0
74-75	37.0714158745664	38.0	38.0	38.0	36.0	38.0
76-77	37.058730113306694	38.0	38.0	38.0	36.0	38.0
78-79	37.046816256327304	38.0	38.0	38.0	36.0	38.0
80-81	37.03799111152196	38.0	38.0	38.0	36.0	38.0
82-83	36.99009296806365	38.0	38.0	38.0	36.0	38.0
84-85	36.98076392981492	38.0	38.0	38.0	36.0	38.0
86-87	36.96736352584547	38.0	38.0	38.0	36.0	38.0
88-89	36.95348480638721	38.0	38.0	38.0	36.0	38.0
90-91	36.94771881073005	38.0	38.0	38.0	35.5	38.0
92-93	36.92918183851747	38.0	38.0	38.0	35.0	38.0
94-95	36.91141050896033	38.0	38.0	38.0	35.0	38.0
96-97	36.89971355222959	38.0	38.0	38.0	35.0	38.0
98-99	36.9135272777781	38.0	38.0	38.0	35.0	38.0
100	35.491176755754566	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	25.0
21	89.0
22	249.0
23	845.0
24	2030.0
25	4184.0
26	7431.0
27	12677.0
28	20072.0
29	29853.0
30	39721.0
31	51184.0
32	65803.0
33	87506.0
34	131738.0
35	243748.0
36	786487.0
37	3852011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.051394223542815	18.178957496901038	14.273984576972513	26.495663702583638
2	14.423017988239014	19.706502056101325	43.29029069199679	22.580189263662874
3	18.122683843311844	25.9672311562093	30.286975245786945	25.623109754691914
4	12.546531280678034	18.160140698672965	37.839213082997446	31.45411493765155
5	13.705563587471312	37.014478814046925	35.460499904604085	13.819457693877682
6	30.245641680932323	37.25233139005536	18.471442509995956	14.030584419016362
7	26.932329620826522	32.219044024185955	22.417591232421568	18.43103512256595
8	24.576612668952922	36.5477697408642	20.775432008573265	18.100185581609615
9	25.40713816365292	17.196042175116922	20.996585613245827	36.400234047984334
10-11	24.65107396313632	26.45981995097887	28.354422266194725	20.53468381969009
12-13	25.535707123657474	23.697339672070434	28.567760527380127	22.19919267689196
14-15	22.641078195041736	25.026519690729803	26.133805802395884	26.198596311832578
16-17	22.29167424148999	28.41112567559615	26.712694104457217	22.58450597845664
18-19	22.33298123680341	27.233276570574798	28.573889106085886	21.859853086535907
20-21	23.01445486194084	26.301495152818582	28.552770788527948	22.13127919671263
22-23	22.809450359074695	26.493565838389348	28.46065847526587	22.23632532727008
24-25	22.747518716122457	26.639904222039316	28.585276894016857	22.027300167821373
26-27	22.716612575699855	26.709639490205134	28.614743212883447	21.95900472121156
28-29	22.683396008981035	26.564069577235916	28.581651889716493	22.170882524066556
30-31	22.631735543395624	26.733731920445265	28.725500502144673	21.909032034014437
32-33	22.591349345873134	26.9110048298719	28.51586429180173	21.981781532453237
34-35	22.89408562342094	26.564752121459218	28.648208648768858	21.89295360635099
36-37	22.372606881213134	26.81937819249792	28.5879548090391	22.220060117249847
38-39	22.29150903963345	26.445345211182353	28.873871028382347	22.38927472080185
40-41	22.55078424603713	26.332519782450138	28.82389863126572	22.292797340247013
42-43	22.63960149097887	26.379705697953593	28.62258947578541	22.358103335282127
44-45	22.408756763538474	26.719771020592482	28.49181128212961	22.379660933739434
46-47	22.789029094444462	26.41231511168025	28.03957616879623	22.75907962507905
48-49	22.68505179936869	26.47257638155221	27.99469426785412	22.84767755122498
50-51	22.461062724303073	26.62267979216898	28.184786928542536	22.73147055498541
52-53	22.583054304860063	26.64173652344702	28.32008559934529	22.455123572347624
54-55	22.56668949657658	26.603362069588126	27.983471321540517	22.846477112294778
56-57	22.79604631640858	26.975438619533215	27.60967425072701	22.61884081333119
58-59	22.397768612374456	27.270367964879494	28.101134182429437	22.230729240316613
60-61	22.681497395555876	27.614555222279186	27.482149937599466	22.22179744456547
62-63	22.336835785935484	27.974398868130308	27.490588656134506	22.198176689799702
64-65	22.295674618076212	27.87035144320036	27.461726749780606	22.37224718894282
66-67	22.358053639742838	27.807947590724936	27.32940603833175	22.504592731200475
68-69	22.397079905387887	27.618814101149525	27.60257682330191	22.38152917016068
70-71	22.58563392621829	27.119731420089543	27.702240721173375	22.59239393251879
72-73	22.647770602080687	26.75601311502154	27.753320351868727	22.84289593102905
74-75	22.551000977894688	26.951640288717805	27.875681713052323	22.62167702033518
76-77	22.76860433692821	26.96819301596073	27.53915446013809	22.724048186972976
78-79	22.70592303902882	26.88748193110042	27.4812676397885	22.925327390082256
80-81	22.722844942152854	26.9446663295727	27.562574528272997	22.76991420000145
82-83	22.824854148564537	26.93535249646824	27.58509034809285	22.654703006874374
84-85	22.835354951457518	26.98570562829092	27.46994804283367	22.7089913774179
86-87	22.73989275629638	27.020254302030356	27.552792983746464	22.687059957926802
88-89	22.842345020627327	27.003155843799455	27.461780749657738	22.692718385915484
90-91	22.904789593441528	27.015529225048844	27.433693499429456	22.645987682080168
92-93	22.9337122280678	27.001043017350835	27.445498723564455	22.61974603101691
94-95	22.73545257606797	27.05696388376029	27.51699068087376	22.69059285929798
96-97	22.910274345932567	27.154732523177955	27.508752948993486	22.426240181896
98-99	23.94197767842387	29.077362262187833	27.671494036867845	19.309166022520444
100	30.80539236734266	40.18370298843843	29.010904644218915	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1019.0
1	907.0
2	1113.0
3	1536.0
4	2066.5
5	3094.5
6	3940.0
7	4969.5
8	6278.5
9	6824.5
10	6634.0
11	6568.0
12	7330.0
13	9316.5
14	12961.0
15	17107.0
16	20463.0
17	22432.0
18	22387.0
19	20608.0
20	18329.5
21	16346.5
22	15649.0
23	17404.5
24	21273.0
25	27029.5
26	35021.0
27	44230.0
28	54067.5
29	65088.0
30	75731.0
31	86501.0
32	99384.5
33	113129.5
34	124895.5
35	136610.0
36	150569.0
37	162319.0
38	171872.5
39	180183.0
40	188233.0
41	196125.0
42	203435.0
43	219044.5
44	236934.0
45	242824.0
46	239299.0
47	233700.5
48	229357.0
49	227801.0
50	226931.5
51	223428.0
52	217422.5
53	208850.5
54	196514.5
55	183310.0
56	169480.5
57	154250.5
58	138391.5
59	119374.0
60	99289.0
61	80877.5
62	64533.0
63	49895.5
64	37440.5
65	28217.0
66	21004.5
67	15804.0
68	12118.0
69	9023.5
70	6649.0
71	4774.0
72	3281.5
73	2155.0
74	1423.0
75	918.5
76	586.5
77	355.0
78	217.5
79	146.5
80	101.5
81	60.0
82	35.0
83	26.5
84	24.5
85	19.5
86	12.0
87	7.5
88	5.5
89	3.5
90	2.5
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010514167137398986
3	7.684150670826354E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.3119468504091964E-4
22-23	9.371524921415078E-6
24-25	2.530529194574733E-4
26-27	2.905756293844699E-4
28-29	0.0026629213240832446
30-31	0.0029846213163156107
32-33	0.002473612759402503
34-35	6.884668597517558E-4
36-37	9.469071975078917E-6
38-39	7.622306336489953E-5
40-41	3.070235277886036E-4
42-43	0.002676262306941973
44-45	2.922237506606692E-4
46-47	8.259141985269623E-4
48-49	0.0010817780540777872
50-51	0.0011214714827324933
52-53	0.0
54-55	0.002949864209612875
56-57	0.05148312948099364
58-59	0.06619839843118382
60-61	0.043830369162798186
62-63	0.00825183287119205
64-65	0.0
66-67	0.0
68-69	2.0705210760519827E-4
70-71	0.0014469738750523698
72-73	0.007741668911115782
74-75	0.012406108061276212
76-77	0.013424511763428544
78-79	0.004091558444560539
80-81	0.007561834358427443
82-83	0.045434162117834596
84-85	0.03819307020827103
86-87	0.008577505726480352
88-89	0.00235386866242206
90-91	0.0027511673955820937
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	48.0
20-21	191.0
22-23	482.0
24-25	494.0
26-27	1287.0
28-29	3375.0
30-31	10589.0
32-33	13498.0
34-35	18462.0
36-37	30420.0
38-39	36790.0
40-41	35474.0
42-43	40016.0
44-45	47032.0
46-47	47822.0
48-49	45731.0
50-51	40232.0
52-53	38299.0
54-55	40284.0
56-57	41209.0
58-59	43715.0
60-61	44666.0
62-63	44871.0
64-65	50996.0
66-67	56642.0
68-69	57931.0
70-71	69583.0
72-73	47500.0
74-75	43919.0
76-77	46217.0
78-79	47939.0
80-81	47304.0
82-83	44126.0
84-85	42883.0
86-87	44603.0
88-89	46216.0
90-91	47580.0
92-93	46115.0
94-95	52980.0
96-97	162099.0
98-99	1229133.0
100-101	2526905.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.42121759980478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.14179542855129	77.00021922139368
2	6.944158867126839	11.863570112729109
3	1.6248637587581563	4.163935221207515
4	0.5868316979240034	2.0051151265131675
5	0.260493192497496	1.1125822839798218
6	0.1390307020130077	0.712570310982405
7	0.0833153142942969	0.49818269142104815
8	0.05277070874833645	0.3606190555910056
9	0.03716336756937235	0.2857086097096395
>10	0.12586338749032844	1.6936441789040555
>50	0.0029210451100226955	0.1587190736313154
>100	7.568266768427359E-4	0.12335844036124831
>500	3.570323992130272E-5	0.021775673576036447
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.748366180890904E-5	0.0	0.0	0.0
3	0.0	3.748366180890904E-5	0.0	0.0	0.0
4	0.0	9.37091545222726E-5	0.0	0.0	0.0
5	0.0	9.37091545222726E-5	0.0	0.0	0.0
6	3.748366180890904E-5	9.37091545222726E-5	0.0	0.0	3.748366180890904E-5
7	3.748366180890904E-5	9.37091545222726E-5	0.0	0.0	3.748366180890904E-5
8	3.748366180890904E-5	9.37091545222726E-5	0.0	0.0	3.748366180890904E-5
9	3.748366180890904E-5	2.2490197085345425E-4	0.0	1.874183090445452E-5	3.748366180890904E-5
10-11	3.748366180890904E-5	2.2490197085345425E-4	0.0	1.874183090445452E-5	3.748366180890904E-5
12-13	3.748366180890904E-5	2.2490197085345425E-4	0.0	1.874183090445452E-5	3.748366180890904E-5
14-15	5.622549271336356E-5	2.6238563266236327E-4	0.0	1.874183090445452E-5	7.496732361781808E-5
16-17	7.496732361781808E-5	2.6238563266236327E-4	0.0	1.874183090445452E-5	7.496732361781808E-5
18-19	7.496732361781808E-5	2.90498379019045E-4	0.0	3.748366180890904E-5	7.496732361781808E-5
20-21	7.496732361781808E-5	3.560947871846359E-4	0.0	7.496732361781808E-5	7.496732361781808E-5
22-23	9.37091545222726E-5	3.654657026368631E-4	0.0	8.433823907004534E-5	8.433823907004534E-5
24-25	9.37091545222726E-5	3.9357844899354493E-4	0.0	1.7804739359231794E-4	1.0308006997449986E-4
26-27	9.37091545222726E-5	4.1232027989799945E-4	0.0	2.811274635668178E-4	1.1245098542672713E-4
28-29	9.37091545222726E-5	4.2169119535022674E-4	0.0	6.372222507514537E-4	1.1245098542672713E-4
30-31	9.37091545222726E-5	4.8728760351581755E-4	0.0	0.0020241177376810883	1.1245098542672713E-4
32-33	9.37091545222726E-5	5.060294344202721E-4	0.0	0.005322679976865084	1.1245098542672713E-4
34-35	1.3119281633118163E-4	5.809967580380902E-4	0.0	0.009605188338532942	1.1245098542672713E-4
36-37	1.3119281633118163E-4	6.559640816559083E-4	0.0	0.016342876548684344	1.1245098542672713E-4
38-39	1.3119281633118163E-4	6.747059125603628E-4	0.0	0.03365095738894809	1.1245098542672713E-4
40-41	1.3119281633118163E-4	7.684150670826354E-4	0.0	0.05179304970446007	1.1245098542672713E-4
42-43	1.3119281633118163E-4	8.058987288915444E-4	0.0	0.06368474141333647	1.1245098542672713E-4
44-45	1.3119281633118163E-4	8.808660525093624E-4	0.0	0.07733816522723158	1.1245098542672713E-4
46-47	1.3119281633118163E-4	9.277206297704987E-4	0.0	0.09229414628898629	1.1245098542672713E-4
48-49	1.3119281633118163E-4	9.652042915794079E-4	0.0	0.10688466164810413	1.3119281633118163E-4
50-51	1.3119281633118163E-4	0.0011057680233628167	0.0	0.12112845313548957	1.3119281633118163E-4
52-53	1.3119281633118163E-4	0.0011057680233628167	0.0	0.15518235988888343	1.3119281633118163E-4
54-55	1.3119281633118163E-4	0.0011151389388150438	0.0	0.1975201559020462	1.3119281633118163E-4
56-57	1.3119281633118163E-4	0.0011432516851717259	0.0	0.2551700277641483	1.3119281633118163E-4
58-59	1.3119281633118163E-4	0.0011619935160761803	0.0	0.2935814102028278	1.3119281633118163E-4
60-61	1.4993464723563616E-4	0.0011713644315284075	0.0	0.3111706185066584	1.3119281633118163E-4
62-63	1.5930556268786342E-4	0.0012088480933373166	0.0	0.3260516322447953	1.4993464723563616E-4
64-65	1.6867647814009068E-4	0.0013119281633118165	0.0	0.3329298841867301	1.4993464723563616E-4
66-67	1.7804739359231794E-4	0.0013212990787640436	0.0	0.3395176377496459	1.4993464723563616E-4
68-69	1.874183090445452E-4	0.001330669994216271	0.0	0.345955456665326	1.4993464723563616E-4
70-71	1.874183090445452E-4	0.0013400409096684983	0.0	0.35128750755764326	1.4993464723563616E-4
72-73	2.0616013994899973E-4	0.0013587827405729526	0.0	0.35562624141202454	1.4993464723563616E-4
74-75	2.0616013994899973E-4	0.00136815365602518	0.0	0.35861556344128503	1.4993464723563616E-4
76-77	2.0616013994899973E-4	0.0014618628105474525	0.0	0.3612769034297176	1.4993464723563616E-4
78-79	2.0616013994899973E-4	0.0015087173878085888	0.0	0.3630854901119974	1.4993464723563616E-4
80-81	2.0616013994899973E-4	0.001518088303260816	0.0	0.3636758577854877	1.4993464723563616E-4
82-83	2.0616013994899973E-4	0.001518088303260816	0.0	0.36429433820533474	1.4993464723563616E-4
84-85	2.0616013994899973E-4	0.001518088303260816	0.0	0.3645473529225449	1.6867647814009068E-4
86-87	2.0616013994899973E-4	0.0015743137959741797	0.0	0.36466917482342387	1.6867647814009068E-4
88	2.0616013994899973E-4	0.001686764781400907	0.0	0.3646785457388761	1.6867647814009068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7190	0.0	49.125267	1
GTATCAA	13450	0.0	37.19263	1
CTTATAC	7285	0.0	34.623604	1
TCAACGC	16410	0.0	30.12007	4
ATCAACG	16465	0.0	30.019453	3
TATCAAC	16905	0.0	29.516321	2
CAACGCA	16805	0.0	29.412098	5
AACGCAG	17190	0.0	28.876242	6
TATACAC	9335	0.0	28.012703	3
TTATACA	9610	0.0	26.774502	2
ACGCAGA	19660	0.0	25.205376	7
CGCAGAG	20185	0.0	24.549799	8
TACACAT	11905	0.0	22.284807	5
TGGTATC	3460	0.0	21.8577	2
ACACATC	11750	0.0	21.428267	6
GTACATG	16625	0.0	21.423649	1
GCAGAGT	23275	0.0	21.290556	9
TACATGG	17005	0.0	20.920061	2
ACATCTC	12230	0.0	20.656342	8
GTGGTAT	3625	0.0	20.51314	1
>>END_MODULE