FastQCFastQC Report
Fri 27 May 2016
SRR2139488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6082711
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89550.050.6079981
GTATCAA164050.038.763831
CTTATAC74050.032.7863351
TCAACGC199250.031.7837944
ATCAACG201150.031.441233
CAACGCA203950.030.9886955
TATCAAC205850.030.9131162
AACGCAG209250.030.2648546
TATACAC95200.027.9599363
ACGCAGA237650.026.5584967
CGCAGAG246750.025.5790358
TTATACA99350.025.2945652
GTGGTAT43850.023.8950881
TGGTATC41800.023.4366022
GTACATG212300.022.851611
GCAGAGT279600.022.6651679
TACATGG215050.022.5197772
ACATGGG214650.021.8647163
TACACAT123550.020.8547865
ACACATC118050.020.7801936
Produced by FastQC (version 0.11.5)