##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6082711
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.451676070094404	28.0	18.0	32.0	18.0	33.0
2	29.91602658748706	32.0	27.0	33.0	25.0	33.0
3	31.786566549027235	33.0	31.0	33.0	29.0	33.0
4	31.934466227312132	33.0	32.0	33.0	31.0	33.0
5	32.85660193292103	33.0	33.0	33.0	32.0	34.0
6	36.71429104555518	38.0	37.0	38.0	34.0	38.0
7	36.87766819761781	38.0	38.0	38.0	35.0	38.0
8	36.8114613697741	38.0	38.0	38.0	34.0	38.0
9	37.19981008468099	38.0	38.0	38.0	36.0	38.0
10-11	37.37334841323219	38.0	38.0	38.0	37.0	38.0
12-13	37.45650911246646	38.0	38.0	38.0	37.0	38.0
14-15	37.40811227099232	38.0	38.0	38.0	37.0	38.0
16-17	37.44794294846491	38.0	38.0	38.0	37.0	38.0
18-19	37.472694001079454	38.0	38.0	38.0	37.0	38.0
20-21	37.48888370126568	38.0	38.0	38.0	37.0	38.0
22-23	37.50535254896861	38.0	38.0	38.0	37.0	38.0
24-25	37.51601777825398	38.0	38.0	38.0	37.0	38.0
26-27	37.49317499085322	38.0	38.0	38.0	37.0	38.0
28-29	37.468607126067866	38.0	38.0	38.0	37.0	38.0
30-31	37.46862180525195	38.0	38.0	38.0	37.0	38.0
32-33	37.45155310673394	38.0	38.0	38.0	37.0	38.0
34-35	37.34855955555948	38.0	38.0	38.0	37.0	38.0
36-37	37.38898930239274	38.0	38.0	38.0	37.0	38.0
38-39	37.36953322997783	38.0	38.0	38.0	37.0	38.0
40-41	37.39028698984953	38.0	38.0	38.0	37.0	38.0
42-43	37.35698092554844	38.0	38.0	38.0	37.0	38.0
44-45	37.31831771118179	38.0	38.0	38.0	37.0	38.0
46-47	37.275097931263936	38.0	38.0	38.0	37.0	38.0
48-49	37.208827404260234	38.0	38.0	38.0	37.0	38.0
50-51	37.16940999622872	38.0	38.0	38.0	37.0	38.0
52-53	37.14197632574589	38.0	38.0	38.0	36.5	38.0
54-55	37.10609707120442	38.0	38.0	38.0	36.0	38.0
56-57	37.05715151086498	38.0	38.0	38.0	36.0	38.0
58-59	37.04120870273777	38.0	38.0	38.0	36.0	38.0
60-61	37.03385709898668	38.0	38.0	38.0	36.0	38.0
62-63	37.03834412906182	38.0	38.0	38.0	36.0	38.0
64-65	37.042604690380514	38.0	38.0	38.0	36.0	38.0
66-67	37.0381962006155	38.0	38.0	38.0	36.0	38.0
68-69	37.02539041154022	38.0	38.0	38.0	36.0	38.0
70-71	37.021295009196706	38.0	38.0	38.0	36.0	38.0
72-73	37.00717923698783	38.0	38.0	38.0	36.0	38.0
74-75	37.00552469234417	38.0	38.0	38.0	36.0	38.0
76-77	36.989772813443935	38.0	38.0	38.0	36.0	38.0
78-79	36.97688987733119	38.0	38.0	38.0	36.0	38.0
80-81	36.968631295608795	38.0	38.0	38.0	36.0	38.0
82-83	36.915218116988065	38.0	38.0	38.0	35.0	38.0
84-85	36.903048568437455	38.0	38.0	38.0	35.0	38.0
86-87	36.89008873843349	38.0	38.0	38.0	35.0	38.0
88-89	36.87250559415939	38.0	38.0	38.0	35.0	38.0
90-91	36.860839262741436	38.0	38.0	38.0	35.0	38.0
92-93	36.83685784236395	38.0	38.0	38.0	35.0	38.0
94-95	36.81913133552786	38.0	38.0	38.0	35.0	38.0
96-97	36.80075146449641	38.0	38.0	38.0	35.0	38.0
98-99	36.81855232470725	38.0	38.0	38.0	35.0	38.0
100	35.34948590242141	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	26.0
21	106.0
22	290.0
23	924.0
24	2241.0
25	4680.0
26	8526.0
27	14534.0
28	23606.0
29	35502.0
30	47319.0
31	60795.0
32	78876.0
33	106161.0
34	160303.0
35	300951.0
36	980749.0
37	4257112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95879288034563	17.952554379124702	14.452355208064299	26.636297532465374
2	14.428696759903218	19.976508361168822	43.469084358790724	22.12571052013724
3	18.123912893372307	26.303048337405016	30.460916769965774	25.112121999256903
4	12.48075077050348	18.355795631257184	38.3376589813325	30.825794616906837
5	13.663512864576338	37.20235927697371	35.54906685522295	13.585061003227015
6	29.91309302710584	37.58368595844846	18.76899954641935	13.734221468026345
7	26.8572845232989	32.46282784107284	22.69539026266413	17.984497372964128
8	24.447569513001685	36.69335926036927	21.017536424137198	17.84153480249185
9	25.566330539129673	17.19016405678323	21.218565208835336	36.02494019525175
10-11	24.4994624929575	26.673197197762644	28.622845964570732	20.20449434470913
12-13	25.369050083096173	24.039831910475446	28.88195740353273	21.70916060289565
14-15	22.48198212934989	25.376061759304363	26.48363533957145	25.658320771774296
16-17	22.010276338954785	28.765668794719986	27.113107954660347	22.11094691166488
18-19	22.005155267116915	27.58267654011509	29.071634341989945	21.340533850778048
20-21	22.704787642426336	26.66969222791897	29.05112568305292	21.574394446601776
22-23	22.523641557845405	26.764176960747406	28.94571262374846	21.766468857658733
24-25	22.373230912002125	27.03067148146455	28.987598420600726	21.6084991859326
26-27	22.246295740083625	27.193385845960965	29.007511008831322	21.55280740512409
28-29	22.1046348016582	27.027435809791633	29.10843816629201	21.759491222258156
30-31	22.54157701850562	26.892719694037524	28.99568487024401	21.57001841721284
32-33	22.15890287143112	27.10877230310325	29.023114696843557	21.70921012862207
34-35	22.304233122667043	26.87032746748076	29.102214046300727	21.723225363551467
36-37	22.105687877737175	27.05049322851548	29.04118779406178	21.80263109968557
38-39	22.02888472255754	26.793824493634737	29.317654698409907	21.859636085397817
40-41	22.254098931690038	26.651311008482654	29.283568063610765	21.81102199621654
42-43	22.321093604441685	26.68603347954162	29.096612164707548	21.896260751309143
44-45	22.136057304123135	26.972015828570896	28.997186353260286	21.894740514045687
46-47	22.46328753575817	26.786518215447874	28.516049061057885	22.234145187736072
48-49	22.32673755869195	26.875666560478678	28.479359132397956	22.31823674843142
50-51	22.156892945982776	27.067447464769618	28.587857021619715	22.187802567627887
52-53	22.36080566940434	27.01416252417837	28.659043204958312	21.965988601458978
54-55	22.30590796095324	27.0074079569253	28.421462740626254	22.26522134149521
56-57	22.47508481451994	27.28157706730317	28.14926498841982	22.09407312975707
58-59	22.15521894973225	27.454367478551276	28.52455447112339	21.86585910059308
60-61	22.35125966495547	27.796005478350427	28.077988469915887	21.77474638677822
62-63	22.107281049873492	28.092253715097197	28.069503321493432	21.730961913535882
64-65	22.121309639620872	27.889465067844665	28.11805498580092	21.871170306733543
66-67	22.181774032784574	27.873266028267235	27.977051861305203	21.967908077642992
68-69	22.196146283501264	27.72823045529853	28.191542587208655	21.884080673991548
70-71	22.407573289591348	27.361861081010495	28.286548193695747	21.944017435702413
72-73	22.390440011425603	27.030998620534408	28.303499061951793	22.275062306088195
74-75	22.22460016892751	27.256495952115266	28.384762234674117	22.134141644283105
76-77	22.41346931593182	27.24699315700154	28.08751427575761	22.252023251309033
78-79	22.406841632891968	27.174065334359792	28.021641696742005	22.39745133600623
80-81	22.357309231030587	27.313494181744698	28.04548609182159	22.283710495403124
82-83	22.472683258865423	27.24626272677989	28.025563885757027	22.255490128597664
84-85	22.460474653440528	27.348509285759782	27.936330034332347	22.254686026467343
86-87	22.38186368595196	27.35933167107231	28.04401074117845	22.21479390179728
88-89	22.488211600139742	27.33692901322076	27.946046563694676	22.22881282294482
90-91	22.50811648569152	27.385956407842784	27.91719223962863	22.188734866837066
92-93	22.578918090047676	27.367022955310727	27.876449167382788	22.177609787258802
94-95	22.409866985531746	27.39584763708599	27.93083824226383	22.263447135118426
96-97	22.568943785348306	27.461147141439024	27.93434430626376	22.03556476694891
98-99	23.55387591993201	29.38049663242786	28.241377406955177	18.82425004068495
100	30.105607955452157	40.30504858772247	29.58934345682538	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1485.0
1	1312.0
2	1486.5
3	1956.0
4	2412.5
5	3401.5
6	4347.0
7	5808.5
8	7843.5
9	8957.0
10	8795.5
11	8440.0
12	9080.5
13	11141.0
14	15298.5
15	20176.5
16	24079.0
17	26405.5
18	26323.0
19	24271.0
20	21472.0
21	19265.5
22	19067.0
23	21355.0
24	26102.5
25	33142.5
26	42296.5
27	52880.0
28	64282.0
29	77589.5
30	91108.0
31	105046.5
32	121294.5
33	138728.5
34	154775.0
35	169250.0
36	185916.5
37	199543.5
38	210920.5
39	221029.5
40	229502.5
41	236812.5
42	241339.0
43	255043.0
44	269197.5
45	271695.0
46	266352.0
47	258647.0
48	252982.5
49	248328.5
50	243664.5
51	236544.5
52	227684.5
53	216961.5
54	203217.0
55	189616.0
56	174059.5
57	157042.0
58	140919.5
59	121207.0
60	100852.0
61	82204.5
62	65265.5
63	50543.0
64	37947.5
65	28494.0
66	21341.0
67	16576.5
68	12969.0
69	9605.5
70	7042.5
71	5095.5
72	3622.5
73	2487.5
74	1718.5
75	1180.5
76	747.0
77	468.0
78	287.5
79	178.0
80	115.0
81	67.5
82	47.0
83	31.5
84	21.0
85	14.5
86	9.5
87	6.5
88	4.0
89	2.5
90	2.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00899270078752714
3	8.384419381423842E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.0686176666009879E-4
22-23	0.0
24-25	2.3019425106300415E-4
26-27	3.371066807801997E-4
28-29	0.0019409580305658125
30-31	0.002790408928254965
32-33	0.002424516513060351
34-35	7.440283048207909E-4
36-37	0.0
38-39	6.673837705280991E-5
40-41	2.1823094608822705E-4
42-43	0.00244922585882527
44-45	2.46735875796223E-4
46-47	5.063229872095089E-4
48-49	0.0012378827081030157
50-51	0.0013613312632953416
52-53	0.0
54-55	0.002930129771995183
56-57	0.050999220830372206
58-59	0.0662276306422282
60-61	0.044897878863146896
62-63	0.008543245791285212
64-65	0.0
66-67	0.0
68-69	1.980526706931089E-4
70-71	0.0014509177436549075
72-73	0.007841433263166525
74-75	0.012768984566026827
76-77	0.013651601278617847
78-79	0.004241884185210223
80-81	0.00799025390289688
82-83	0.04493420022411174
84-85	0.03767741580370939
86-87	0.008774905926473965
88-89	0.00243167338433908
90-91	0.0026706271077553525
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	247.0
22-23	509.0
24-25	544.0
26-27	1240.0
28-29	3292.0
30-31	10588.0
32-33	13820.0
34-35	19231.0
36-37	30574.0
38-39	36897.0
40-41	35792.0
42-43	41272.0
44-45	49309.0
46-47	51229.0
48-49	47416.0
50-51	42308.0
52-53	39758.0
54-55	42621.0
56-57	43256.0
58-59	46974.0
60-61	47948.0
62-63	47447.0
64-65	53976.0
66-67	59091.0
68-69	60967.0
70-71	71967.0
72-73	50703.0
74-75	48541.0
76-77	50515.0
78-79	52952.0
80-81	52120.0
82-83	48658.0
84-85	48174.0
86-87	49558.0
88-89	51897.0
90-91	52592.0
92-93	51572.0
94-95	59481.0
96-97	184922.0
98-99	1406135.0
100-101	2976575.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.6845749730835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.6102383134806	78.54509996413871
2	6.765104158732456	11.728603572967252
3	1.5101944094837352	3.927316815384732
4	0.5207554159412754	1.8056584758320309
5	0.2399225342826236	1.0398791455377139
6	0.12000313602057308	0.6241452504828302
7	0.07418692518334007	0.4501603454654444
8	0.04214834484353054	0.29228890868643204
9	0.026690564799396035	0.208229423888447
>10	0.08823274196511342	1.1857130554756814
>50	0.0021044942749607145	0.12764267710456187
>100	3.9014070802509014E-4	0.05037265135585786
>500	2.8820284441751947E-5	0.014889713680322363
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6440038002791847E-5	0.0
2	0.0	0.0	0.0	1.6440038002791847E-5	0.0
3	0.0	0.0	0.0	3.2880076005583694E-5	0.0
4	0.0	0.0	0.0	6.576015201116739E-5	0.0
5	0.0	1.6440038002791847E-5	0.0	6.576015201116739E-5	0.0
6	0.0	3.2880076005583694E-5	0.0	6.576015201116739E-5	0.0
7	0.0	3.2880076005583694E-5	0.0	6.576015201116739E-5	0.0
8	0.0	3.2880076005583694E-5	0.0	6.576015201116739E-5	0.0
9	4.9320114008375544E-5	9.864022801675109E-5	0.0	6.576015201116739E-5	0.0
10-11	5.7540133009771465E-5	9.864022801675109E-5	0.0	9.864022801675109E-5	0.0
12-13	6.576015201116739E-5	9.864022801675109E-5	0.0	9.864022801675109E-5	0.0
14-15	6.576015201116739E-5	1.3152030402233477E-4	0.0	1.1508026601954293E-4	0.0
16-17	6.576015201116739E-5	1.4796034202512663E-4	0.0	1.1508026601954293E-4	0.0
18-19	7.398017101256332E-5	1.5618036102652255E-4	0.0	1.2330028502093886E-4	0.0
20-21	8.220019001395923E-5	1.6440038002791846E-4	0.0	1.726203990293144E-4	0.0
22-23	8.220019001395923E-5	2.13720494036294E-4	0.0	2.466005700418777E-4	0.0
24-25	1.06860247018147E-4	2.466005700418777E-4	0.0	3.041407030516492E-4	0.0
26-27	1.3152030402233477E-4	2.7126062704606546E-4	0.0	3.781208740642125E-4	0.0
28-29	1.3152030402233477E-4	2.794806460474614E-4	0.0	6.41161482108882E-4	0.0
30-31	1.3974032302373072E-4	2.9592068405025326E-4	0.0	0.001841284256312687	0.0
32-33	1.4796034202512663E-4	2.9592068405025326E-4	0.0	0.004512790431766361	0.0
34-35	1.5618036102652255E-4	3.534608170600247E-4	0.0	0.007841898127331711	0.0
36-37	1.6440038002791846E-4	4.110009500697962E-4	0.0	0.01334931085826698	0.0
38-39	1.8084041803071032E-4	4.438810260753799E-4	0.0	0.02730690312263726	0.0
40-41	1.9728045603350217E-4	4.932011400837554E-4	0.0	0.04303179947230766	0.0
42-43	1.9728045603350217E-4	5.014211590851514E-4	0.0	0.05326572312904558	0.0
44-45	2.055004750348981E-4	5.34301235090735E-4	0.0	0.06472442961699151	0.0
46-47	2.13720494036294E-4	5.589612920949228E-4	0.0	0.07725173857511888	8.220019001395923E-6
48-49	2.13720494036294E-4	5.589612920949228E-4	0.0	0.08966396726722674	1.6440038002791847E-5
50-51	2.2194051303768992E-4	6.000613871019024E-4	0.0	0.10264337727043091	1.6440038002791847E-5
52-53	2.3016053203908586E-4	6.082814061032984E-4	0.0	0.1346932313568736	1.6440038002791847E-5
54-55	2.3016053203908586E-4	6.165014251046943E-4	0.0	0.17373010159450286	1.6440038002791847E-5
56-57	2.3016053203908586E-4	6.41161482108882E-4	0.0	0.23219087673243066	1.6440038002791847E-5
58-59	2.3016053203908586E-4	6.41161482108882E-4	0.0	0.27055370541194546	1.6440038002791847E-5
60-61	2.3016053203908586E-4	6.41161482108882E-4	0.0	0.2843386772772864	1.6440038002791847E-5
62-63	2.3016053203908586E-4	6.576015201116738E-4	0.0	0.29429312028797683	1.6440038002791847E-5
64-65	2.3016053203908586E-4	6.904815961172576E-4	0.0	0.3003677143300084	1.6440038002791847E-5
66-67	2.3016053203908586E-4	7.069216341200494E-4	0.0	0.3063765482200289	1.6440038002791847E-5
68-69	2.3016053203908586E-4	7.069216341200494E-4	0.0	0.3123031819200353	1.6440038002791847E-5
70-71	2.3016053203908586E-4	7.069216341200494E-4	0.0	0.3172023132448673	1.6440038002791847E-5
72-73	2.466005700418777E-4	7.069216341200494E-4	0.0	0.3213863029165778	1.6440038002791847E-5
74-75	2.466005700418777E-4	7.069216341200494E-4	0.0	0.3242386495100622	1.6440038002791847E-5
76-77	2.466005700418777E-4	7.315816911242372E-4	0.0	0.3272800565405787	1.6440038002791847E-5
78-79	2.466005700418777E-4	7.562417481284249E-4	0.0	0.32914600085389556	1.6440038002791847E-5
80-81	2.466005700418777E-4	7.562417481284249E-4	0.0	0.32978716233600447	1.6440038002791847E-5
82-83	2.466005700418777E-4	7.562417481284249E-4	0.0	0.3301981632860742	1.6440038002791847E-5
84-85	2.466005700418777E-4	7.562417481284249E-4	0.0	0.33046942391312034	1.6440038002791847E-5
86-87	2.466005700418777E-4	7.562417481284249E-4	0.0	0.3305598441221357	1.6440038002791847E-5
88	2.466005700418777E-4	7.726817861312169E-4	0.0	0.3305598441221357	1.6440038002791847E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8955	0.0	50.607998	1
GTATCAA	16405	0.0	38.76383	1
CTTATAC	7405	0.0	32.786335	1
TCAACGC	19925	0.0	31.783794	4
ATCAACG	20115	0.0	31.44123	3
CAACGCA	20395	0.0	30.988695	5
TATCAAC	20585	0.0	30.913116	2
AACGCAG	20925	0.0	30.264854	6
TATACAC	9520	0.0	27.959936	3
ACGCAGA	23765	0.0	26.558496	7
CGCAGAG	24675	0.0	25.579035	8
TTATACA	9935	0.0	25.294565	2
GTGGTAT	4385	0.0	23.895088	1
TGGTATC	4180	0.0	23.436602	2
GTACATG	21230	0.0	22.85161	1
GCAGAGT	27960	0.0	22.665167	9
TACATGG	21505	0.0	22.519777	2
ACATGGG	21465	0.0	21.864716	3
TACACAT	12355	0.0	20.854786	5
ACACATC	11805	0.0	20.780193	6
>>END_MODULE