FastQCFastQC Report
Fri 27 May 2016
SRR2139487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5743651
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75900.050.64851
GTATCAA136950.036.6619221
TCAACGC159300.030.8766274
ATCAACG160000.030.7152613
CTTATAC101100.030.461171
CAACGCA162550.030.2331545
TATCAAC167850.029.7355692
AACGCAG167500.029.4157716
TATACAC119650.027.4769863
ACGCAGA192500.025.5955417
TTATACA125450.025.2935432
CGCAGAG197500.024.9475528
GTGGTAT36000.023.7166481
GCAGAGT225550.021.7508499
TGGTATC35650.021.6856082
TACACAT155000.021.5391185
ACACATC148300.020.8510196
GAGTACT135450.020.43019312-13
ACATCTC152100.020.1625338
ATACACA175900.019.8009054
Produced by FastQC (version 0.11.5)