##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5743651
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.644084398582017	28.0	18.0	32.0	18.0	33.0
2	29.99378809750105	32.0	28.0	33.0	25.0	33.0
3	31.796976348319212	33.0	31.0	33.0	29.0	33.0
4	31.92335937542166	33.0	32.0	33.0	31.0	33.0
5	32.84057561993234	33.0	33.0	33.0	32.0	34.0
6	36.68985633005905	38.0	37.0	38.0	34.0	38.0
7	36.86743519061308	38.0	38.0	38.0	35.0	38.0
8	36.802788330976234	38.0	38.0	38.0	34.0	38.0
9	37.19267100316506	38.0	38.0	38.0	36.0	38.0
10-11	37.37004102442854	38.0	38.0	38.0	37.0	38.0
12-13	37.449267112503875	38.0	38.0	38.0	37.0	38.0
14-15	37.39894110906111	38.0	38.0	38.0	37.0	38.0
16-17	37.434094620303355	38.0	38.0	38.0	37.0	38.0
18-19	37.45934902729988	38.0	38.0	38.0	37.0	38.0
20-21	37.47656862739239	38.0	38.0	38.0	37.0	38.0
22-23	37.491212851888996	38.0	38.0	38.0	37.0	38.0
24-25	37.50088236177905	38.0	38.0	38.0	37.0	38.0
26-27	37.475830787011986	38.0	38.0	38.0	37.0	38.0
28-29	37.44459114194085	38.0	38.0	38.0	37.0	38.0
30-31	37.441067532003885	38.0	38.0	38.0	37.0	38.0
32-33	37.427912419503954	38.0	38.0	38.0	37.0	38.0
34-35	37.324074303264474	38.0	38.0	38.0	37.0	38.0
36-37	37.36119835796633	38.0	38.0	38.0	37.0	38.0
38-39	37.33218328113884	38.0	38.0	38.0	37.0	38.0
40-41	37.37310277387786	38.0	38.0	38.0	37.0	38.0
42-43	37.343954001071786	38.0	38.0	38.0	37.0	38.0
44-45	37.30631880181468	38.0	38.0	38.0	37.0	38.0
46-47	37.269552092148274	38.0	38.0	38.0	37.0	38.0
48-49	37.208663055371744	38.0	38.0	38.0	37.0	38.0
50-51	37.17211050523173	38.0	38.0	38.0	37.0	38.0
52-53	37.1410552711503	38.0	38.0	38.0	36.5	38.0
54-55	37.10256620443297	38.0	38.0	38.0	36.0	38.0
56-57	37.04851376489738	38.0	38.0	38.0	36.0	38.0
58-59	37.03203110510682	38.0	38.0	38.0	36.0	38.0
60-61	37.0208645623575	38.0	38.0	38.0	36.0	38.0
62-63	37.023975766032265	38.0	38.0	38.0	36.0	38.0
64-65	37.02828693668502	38.0	38.0	38.0	36.0	38.0
66-67	37.02044761336877	38.0	38.0	38.0	36.0	38.0
68-69	37.01006218251803	38.0	38.0	38.0	36.0	38.0
70-71	37.00643913840794	38.0	38.0	38.0	36.0	38.0
72-73	36.99607885027514	38.0	38.0	38.0	36.0	38.0
74-75	36.99194335504346	38.0	38.0	38.0	36.0	38.0
76-77	36.974753866714636	38.0	38.0	38.0	36.0	38.0
78-79	36.95626723962828	38.0	38.0	38.0	36.0	38.0
80-81	36.93904098744356	38.0	38.0	38.0	35.5	38.0
82-83	36.88351687238101	38.0	38.0	38.0	35.0	38.0
84-85	36.87255388330034	38.0	38.0	38.0	35.0	38.0
86-87	36.855824925505075	38.0	38.0	38.0	35.0	38.0
88-89	36.83237915192225	38.0	38.0	38.0	35.0	38.0
90-91	36.81912166235551	38.0	38.0	38.0	35.0	38.0
92-93	36.79510875543494	38.0	38.0	38.0	35.0	38.0
94-95	36.768757094077316	38.0	38.0	38.0	35.0	38.0
96-97	36.7531811251029	38.0	38.0	38.0	35.0	38.0
98-99	36.767767774071764	38.0	38.0	38.0	35.0	38.0
100	35.26805273673203	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	16.0
21	80.0
22	327.0
23	939.0
24	2264.0
25	4662.0
26	8588.0
27	14720.0
28	22994.0
29	33578.0
30	45473.0
31	58048.0
32	75446.0
33	102474.0
34	156635.0
35	292948.0
36	940752.0
37	3983698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.286222125961345	18.250830351635223	14.535597653826807	26.927349868576623
2	14.118275538861457	20.048872721802113	43.72938497646124	22.103466762875186
3	17.912320315564546	26.24326403720586	30.647932111001253	25.196483536228342
4	12.29139792790335	18.576981783886243	38.48712256367944	30.644497724530968
5	13.519606257413622	37.26648781410988	35.80463019079676	13.409275737679744
6	29.758214766182693	37.88377810559869	18.74823174318913	13.609775385029488
7	26.512230635183094	32.59160419043566	22.75281001578961	18.143355158591636
8	24.169539548973294	36.99098360955427	21.14761151051831	17.691865330954126
9	25.154609846594088	17.472510081131322	21.318025764448432	36.054854307826155
10-11	24.22165796633535	26.87311607198975	28.791312355155284	20.113913606519613
12-13	25.062299223960512	24.117943447469216	28.97898914819163	21.840768180378646
14-15	22.24087083285527	25.57374220682977	26.497422980609368	25.687963979705593
16-17	21.890275018450808	28.985439749037678	27.12882450552793	21.995460726983588
18-19	21.902279577920037	27.765727757483873	28.96163085117811	21.370361813417983
20-21	22.50387736724849	26.934705274821248	28.890634216799672	21.670783141130595
22-23	22.336498895857503	27.12104093571528	28.747968745184664	21.79449142324255
24-25	22.306967142180042	27.20126777254898	28.864035190056182	21.627729895214788
26-27	22.263956691195947	27.318020426690005	28.846399991362482	21.57162289075157
28-29	21.892902148249078	27.088986796112312	28.936299490191065	22.081811565447545
30-31	22.48342546228731	27.0716679253415	28.915481996910657	21.529424615460528
32-33	22.409515831317663	26.999164209911967	29.072253786712555	21.519066172057812
34-35	21.947953451475556	27.112861335972738	29.338577703045576	21.60060750950613
36-37	22.223143178516285	27.166457634980134	28.953456617054858	21.656942569448724
38-39	21.887808039383355	27.093091602739644	29.21066829373124	21.808432064145762
40-41	22.10225815351401	26.946034051887008	29.19929426085604	21.752413533742942
42-43	22.231823544551876	26.943013997671205	29.067498360690387	21.757664097086536
44-45	21.950221430885943	27.27564348684413	28.972944907612984	21.801190174656945
46-47	22.35800391085494	26.973746799864063	28.43970288864132	22.228546400639676
48-49	22.244483327133537	27.034604062475605	28.487570551414702	22.23334205897616
50-51	21.978795929365205	27.300428476662532	28.621337187619844	22.099438406352423
52-53	22.137030278305467	27.319715206172702	28.739169077373646	21.80408543814819
54-55	22.13313927812553	27.322057868638872	28.416785625102968	22.12801722813263
56-57	22.321516574598203	27.69136302775095	28.008870340408176	21.978250057242676
58-59	21.845764468673845	27.93724417978452	28.47105311234315	21.74593823919849
60-61	22.32547983714711	28.251961644920993	27.965973882903235	21.456584635028662
62-63	21.809608913719455	28.562822124562764	27.935367273748955	21.692201687968822
64-65	21.775736420154235	28.417972965552234	27.99297616883663	21.813314445456903
66-67	21.91452436530162	28.35316165230658	28.04506020745629	21.68725377493551
68-69	21.868357820553115	28.39681589164742	28.136115592594997	21.59871069520447
70-71	22.104590685600463	27.919636429825673	27.995703379705443	21.980069504868418
72-73	22.11956376301743	27.41402166775424	28.175624908654136	22.290789660574198
74-75	22.066636564043332	27.577235681088485	28.493920850058167	21.862206904810016
76-77	22.208434302264187	27.422650783710644	28.135719037693224	22.233195876331948
78-79	22.14255658131608	27.36227736933996	27.880497579631236	22.61466846971273
80-81	22.370099214317953	27.586388742669705	27.987359802418133	22.056152240594212
82-83	22.456578962480506	27.389573750157382	27.97700839566935	22.176838891692764
84-85	22.276784829999695	27.40366605714183	27.84736540924491	22.47218370361356
86-87	22.164611966373492	27.673020693490912	27.920793917712807	22.241573422422785
88-89	22.485104388521197	27.674477286656902	27.835650701499215	22.00476762332268
90-91	22.529671639578936	27.667939717747764	27.81328172445704	21.989106918216258
92-93	22.342300034564218	27.423994424020133	27.824139536610936	22.409566004804713
94-95	22.394566082540265	27.77056253538429	27.84445776528711	21.99041361678833
96-97	22.743732819585087	27.578624627023473	27.92600380612269	21.751638747268753
98-99	23.43565072763057	29.608285480163204	28.289940264495744	18.666123527710486
100	29.755209449575464	40.67721765696672	29.56757289345782	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	986.0
1	919.5
2	1131.0
3	1516.5
4	1961.0
5	2821.5
6	3557.5
7	4515.5
8	5766.0
9	6492.0
10	6567.5
11	6500.0
12	7236.5
13	9149.5
14	12801.5
15	17162.5
16	20666.5
17	22826.0
18	23163.0
19	21978.0
20	20021.0
21	18513.0
22	18797.5
23	21341.0
24	26246.5
25	33286.0
26	42939.0
27	54040.5
28	65393.5
29	78507.0
30	91640.0
31	104455.5
32	118833.0
33	134075.5
34	149342.0
35	164477.5
36	179786.0
37	192698.5
38	202442.5
39	210473.5
40	218337.0
41	225851.5
42	230291.5
43	233992.0
44	239207.5
45	244788.5
46	261088.0
47	272168.5
48	267163.0
49	258214.0
50	244500.5
51	229823.0
52	216043.5
53	202804.5
54	188425.0
55	173753.5
56	157784.5
57	141207.5
58	125534.0
59	106935.5
60	87516.5
61	70257.5
62	55275.5
63	42740.0
64	31682.0
65	23445.5
66	17635.5
67	13440.5
68	10306.0
69	7715.0
70	5725.0
71	4144.0
72	2929.5
73	2072.5
74	1431.5
75	933.5
76	597.0
77	356.0
78	215.0
79	132.5
80	90.0
81	62.0
82	37.5
83	22.0
84	15.0
85	11.0
86	8.0
87	7.0
88	6.0
89	5.0
90	3.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009924001301611118
3	6.964211439727101E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.1316954913860998E-4
22-23	0.0
24-25	2.176563340561555E-4
26-27	2.699217723168056E-4
28-29	0.0023516226283232565
30-31	0.0028418538825085203
32-33	0.0022272100080860825
34-35	6.742665556082515E-4
36-37	0.0
38-39	5.304105120998572E-5
40-41	2.1352558227759018E-4
42-43	0.0025970360117550805
44-45	2.7070268732874107E-4
46-47	6.645805513505916E-4
48-49	0.0011572823923341614
50-51	0.0011853883475769782
52-53	0.0
54-55	0.003091930906151909
56-57	0.05009067093460401
58-59	0.0653442169176311
60-61	0.04331503782696204
62-63	0.007978401120659988
64-65	0.0
66-67	0.0
68-69	1.3024478204337232E-4
70-71	0.0015227370528520712
72-73	0.007626154973966613
74-75	0.012077011004214898
76-77	0.013035127674982421
78-79	0.0040083364915772975
80-81	0.00789896357452431
82-83	0.043678787087844395
84-85	0.03672434186084877
86-87	0.008819449295018758
88-89	0.0023568361652600116
90-91	0.0024061170952134423
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	31.0
20-21	157.0
22-23	385.0
24-25	459.0
26-27	1276.0
28-29	3182.0
30-31	10412.0
32-33	13794.0
34-35	18558.0
36-37	30442.0
38-39	36169.0
40-41	35919.0
42-43	40140.0
44-45	47902.0
46-47	49328.0
48-49	45835.0
50-51	40637.0
52-53	38381.0
54-55	41933.0
56-57	43201.0
58-59	25517.0
60-61	49996.0
62-63	49352.0
64-65	54377.0
66-67	60039.0
68-69	61421.0
70-71	73356.0
72-73	49315.0
74-75	46166.0
76-77	48209.0
78-79	50118.0
80-81	49351.0
82-83	46453.0
84-85	45943.0
86-87	47322.0
88-89	49535.0
90-91	50776.0
92-93	49411.0
94-95	57407.0
96-97	193474.0
98-99	1307064.0
100-101	2780908.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.48221440577768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44878826634218	78.22211499592593
2	6.86581813647001	11.875423122985529
3	1.536477863789443	3.9863402413790965
4	0.5649238509895058	1.9542346241684816
5	0.23174471703956445	1.0020898153210949
6	0.12303302902780086	0.6384101277224725
7	0.07122819777942299	0.4311980590468038
8	0.0406795269494158	0.28144444572519906
9	0.02743730248969831	0.21355548089672405
>10	0.08738144779769623	1.1504105668974707
>50	0.0017774799415759854	0.10785271991346884
>100	6.79784584101055E-4	0.11655273533075648
>500	3.0396799600033777E-5	0.020373064687011735
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.741052859931775E-5	1.741052859931775E-5
2	0.0	1.741052859931775E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
3	0.0	1.741052859931775E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
4	0.0	1.741052859931775E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
5	0.0	1.741052859931775E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
6	1.044631715959065E-4	1.741052859931775E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
7	1.044631715959065E-4	1.741052859931775E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
8	1.044631715959065E-4	3.48210571986355E-5	0.0	1.741052859931775E-5	1.741052859931775E-5
9	1.2187370019522426E-4	8.705264299658876E-5	0.0	1.741052859931775E-5	3.48210571986355E-5
10-11	1.2187370019522426E-4	8.705264299658876E-5	0.0	3.48210571986355E-5	3.48210571986355E-5
12-13	1.2187370019522426E-4	8.705264299658876E-5	0.0	5.223158579795325E-5	3.48210571986355E-5
14-15	1.2187370019522426E-4	1.044631715959065E-4	0.0	6.9642114397271E-5	3.48210571986355E-5
16-17	1.8281055029283638E-4	1.044631715959065E-4	0.0	8.705264299658876E-5	3.48210571986355E-5
18-19	1.9151581459249525E-4	1.2187370019522425E-4	0.0	8.705264299658876E-5	6.0936850097612123E-5
20-21	2.0022107889215414E-4	1.39284228794542E-4	0.0	8.705264299658876E-5	6.9642114397271E-5
22-23	2.08926343191813E-4	1.39284228794542E-4	0.0	1.0446317159590652E-4	9.575790729624762E-5
24-25	2.2633687179113076E-4	1.39284228794542E-4	0.0	1.39284228794542E-4	1.044631715959065E-4
26-27	2.2633687179113076E-4	1.39284228794542E-4	0.0	1.7410528599317752E-4	1.044631715959065E-4
28-29	2.2633687179113076E-4	1.479894930942009E-4	0.0	4.7878953648123815E-4	1.044631715959065E-4
30-31	2.2633687179113076E-4	1.7410528599317752E-4	0.0	0.0012796738520498548	1.044631715959065E-4
32-33	2.2633687179113076E-4	1.7410528599317752E-4	0.0	0.003551747834260821	1.1316843589556538E-4
34-35	2.2633687179113076E-4	2.2633687179113076E-4	0.0	0.006555064017643133	1.2187370019522426E-4
36-37	2.2633687179113076E-4	2.611579289897663E-4	0.0	0.011595412047145622	1.2187370019522426E-4
38-39	2.4374740039044852E-4	3.4821057198635504E-4	0.0	0.025993919198781405	1.39284228794542E-4
40-41	2.611579289897663E-4	3.743263648853316E-4	0.0	0.04121942645888478	1.39284228794542E-4
42-43	2.78568457589084E-4	4.4396847928260263E-4	0.0	0.05071686980981261	1.479894930942009E-4
44-45	2.872737218887429E-4	5.136105936798737E-4	0.0	0.0611980080266019	1.5669475739385976E-4
46-47	3.133895147877195E-4	5.397263865788502E-4	0.0	0.07226239895146833	1.5669475739385976E-4
48-49	3.133895147877195E-4	5.484316508785091E-4	0.0	0.08368370571262077	1.5669475739385976E-4
50-51	3.133895147877195E-4	6.703053510737334E-4	0.0	0.09466974925879026	1.6540002169351863E-4
52-53	3.133895147877195E-4	6.790106153733923E-4	0.0	0.1286638063489582	1.9151581459249525E-4
54-55	3.133895147877195E-4	6.790106153733923E-4	0.0	0.17161558040347508	2.08926343191813E-4
56-57	3.133895147877195E-4	6.964211439727101E-4	0.0	0.2247176926313942	2.08926343191813E-4
58-59	3.133895147877195E-4	7.05126408272369E-4	0.0	0.434262109588483	2.1763160749147187E-4
60-61	3.133895147877195E-4	7.138316725720278E-4	0.0	0.6220259552678253	2.2633687179113076E-4
62-63	3.4821057198635504E-4	7.138316725720278E-4	0.0	0.628998871971852	2.4374740039044852E-4
64-65	3.4821057198635504E-4	8.182948441679343E-4	0.0	0.6356322833681921	2.4374740039044852E-4
66-67	3.4821057198635504E-4	8.444106370669109E-4	0.0	0.642143821064337	2.4374740039044852E-4
68-69	3.4821057198635504E-4	8.531159013665698E-4	0.0	0.648498664003088	2.4374740039044852E-4
70-71	3.5691583628601393E-4	8.531159013665698E-4	0.0	0.6539481594546743	2.4374740039044852E-4
72-73	4.17852686383626E-4	8.705264299658875E-4	0.0	0.6579525810325175	2.78568457589084E-4
74-75	4.17852686383626E-4	9.227580157638408E-4	0.0	0.6607992024585059	2.78568457589084E-4
76-77	4.17852686383626E-4	9.924001301611118E-4	0.0	0.6640636765708781	2.78568457589084E-4
78-79	4.17852686383626E-4	0.0010185159230600884	0.0	0.6662225821171934	2.78568457589084E-4
80-81	4.265579506832849E-4	0.0010272211873597473	0.0	0.6669799401112637	2.78568457589084E-4
82-83	4.352632149829438E-4	0.0010272211873597473	0.0	0.6676241296694385	2.78568457589084E-4
84-85	4.4396847928260263E-4	0.001062042244558383	0.0	0.6678765823341286	2.78568457589084E-4
86-87	4.7878953648123815E-4	0.0010794527731577005	0.0	0.6679723402414248	2.78568457589084E-4
88	4.8749480078089704E-4	0.0011316843589556538	0.0	0.6679897507700242	2.78568457589084E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7590	0.0	50.6485	1
GTATCAA	13695	0.0	36.661922	1
TCAACGC	15930	0.0	30.876627	4
ATCAACG	16000	0.0	30.715261	3
CTTATAC	10110	0.0	30.46117	1
CAACGCA	16255	0.0	30.233154	5
TATCAAC	16785	0.0	29.735569	2
AACGCAG	16750	0.0	29.415771	6
TATACAC	11965	0.0	27.476986	3
ACGCAGA	19250	0.0	25.595541	7
TTATACA	12545	0.0	25.293543	2
CGCAGAG	19750	0.0	24.947552	8
GTGGTAT	3600	0.0	23.716648	1
GCAGAGT	22555	0.0	21.750849	9
TGGTATC	3565	0.0	21.685608	2
TACACAT	15500	0.0	21.539118	5
ACACATC	14830	0.0	20.851019	6
GAGTACT	13545	0.0	20.430193	12-13
ACATCTC	15210	0.0	20.162533	8
ATACACA	17590	0.0	19.800905	4
>>END_MODULE