FastQCFastQC Report
Fri 27 May 2016
SRR2139486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4310009
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT41750.062.13606394
GGTATCA59900.044.862291
TATCTCG52700.043.97472494
GTATCAA104250.035.407041
TATGCCG25600.030.05929486-87
TCAACGC122600.029.43524
ATCAACG122800.029.3527053
TCTCGTA25150.029.31107982-83
GCCGTCT27300.029.2717990-91
CCGTCTT27250.028.91678290-91
CAACGCA125050.028.82395
ATGCCGT27500.028.50232788-89
TATCAAC128350.028.4890172
TGCCGTC27550.028.25164288-89
CTCGTAT27000.027.99020882-83
AACGCAG129300.027.8764766
TCGTATG27800.027.77078684-85
CGTATGC29350.026.57818684-85
CTATCTC67350.025.78436992-93
ACGCAGA144000.025.0908537
Produced by FastQC (version 0.11.5)