##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4310009
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.806794602981107	28.0	18.0	32.0	18.0	33.0
2	30.164130283718666	32.0	29.0	33.0	25.0	33.0
3	31.89602504310316	33.0	31.0	33.0	29.0	33.0
4	32.00853524899832	33.0	32.0	33.0	31.0	33.0
5	32.89126588830789	33.0	33.0	33.0	32.0	34.0
6	36.77494339338966	38.0	37.0	38.0	34.0	38.0
7	36.93847785468662	38.0	38.0	38.0	35.0	38.0
8	36.85970655745731	38.0	38.0	38.0	35.0	38.0
9	37.22181183380359	38.0	38.0	38.0	36.0	38.0
10-11	37.39818478337284	38.0	38.0	38.0	37.0	38.0
12-13	37.472672562864716	38.0	38.0	38.0	37.0	38.0
14-15	37.4236639645068	38.0	38.0	38.0	37.0	38.0
16-17	37.46350239639871	38.0	38.0	38.0	37.0	38.0
18-19	37.485132049608254	38.0	38.0	38.0	37.0	38.0
20-21	37.499004741022944	38.0	38.0	38.0	37.0	38.0
22-23	37.51439766996579	38.0	38.0	38.0	37.5	38.0
24-25	37.52790322997622	38.0	38.0	38.0	38.0	38.0
26-27	37.50300872622378	38.0	38.0	38.0	37.5	38.0
28-29	37.47729596786696	38.0	38.0	38.0	37.0	38.0
30-31	37.47917262638329	38.0	38.0	38.0	37.0	38.0
32-33	37.46699104504931	38.0	38.0	38.0	37.0	38.0
34-35	37.370233602726856	38.0	38.0	38.0	37.0	38.0
36-37	37.41172245268342	38.0	38.0	38.0	37.0	38.0
38-39	37.41349964570717	38.0	38.0	38.0	37.0	38.0
40-41	37.40677582019349	38.0	38.0	38.0	37.0	38.0
42-43	37.36982309292755	38.0	38.0	38.0	37.0	38.0
44-45	37.33800182330826	38.0	38.0	38.0	37.0	38.0
46-47	37.301523791188416	38.0	38.0	38.0	37.0	38.0
48-49	37.242228856422884	38.0	38.0	38.0	37.0	38.0
50-51	37.20684926970375	38.0	38.0	38.0	37.0	38.0
52-53	37.17992629070102	38.0	38.0	38.0	37.0	38.0
54-55	37.14941567566805	38.0	38.0	38.0	36.5	38.0
56-57	37.10063221063797	38.0	38.0	38.0	36.0	38.0
58-59	37.09184165336224	38.0	38.0	38.0	36.0	38.0
60-61	37.08775956150335	38.0	38.0	38.0	36.0	38.0
62-63	37.09238807415809	38.0	38.0	38.0	36.0	38.0
64-65	37.095734053550835	38.0	38.0	38.0	36.0	38.0
66-67	37.08810059626969	38.0	38.0	38.0	36.0	38.0
68-69	37.07661339013159	38.0	38.0	38.0	36.0	38.0
70-71	37.06933417783051	38.0	38.0	38.0	36.0	38.0
72-73	37.05989636930353	38.0	38.0	38.0	36.0	38.0
74-75	37.0477572067919	38.0	38.0	38.0	36.0	38.0
76-77	37.02760677093303	38.0	38.0	38.0	36.0	38.0
78-79	37.006494533084755	38.0	38.0	38.0	36.0	38.0
80-81	36.989615041997865	38.0	38.0	38.0	36.0	38.0
82-83	36.91015404697663	38.0	38.0	38.0	35.0	38.0
84-85	36.925113611119116	38.0	38.0	38.0	35.0	38.0
86-87	36.91461871682747	38.0	38.0	38.0	35.0	38.0
88-89	36.89285597339769	38.0	38.0	38.0	35.0	38.0
90-91	36.888278055586575	38.0	38.0	38.0	35.0	38.0
92-93	36.86705084395881	38.0	38.0	38.0	35.0	38.0
94-95	36.81357827913058	38.0	38.0	38.0	35.0	38.0
96-97	36.745739371166025	38.0	38.0	38.0	34.5	38.0
98-99	36.7820905900832	38.0	38.0	38.0	34.5	38.0
100	35.3396134110936	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	14.0
21	60.0
22	216.0
23	688.0
24	1534.0
25	3364.0
26	6050.0
27	10196.0
28	16529.0
29	24018.0
30	32050.0
31	40995.0
32	53584.0
33	72212.0
34	109559.0
35	203996.0
36	653757.0
37	3081182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.20671209735293	18.157317072887782	14.421965244156102	26.214005585603182
2	14.4666466492574	20.095382800466496	43.10246330154525	22.335507248730853
3	18.358149011004002	25.92851423569082	30.11505629844428	25.598280454860895
4	12.53259563959147	17.93478853524436	38.7943041418243	30.738311683339877
5	13.76229144765127	37.25064147197836	35.48115560779571	13.505911472574649
6	30.318915807368384	37.65384248617578	18.673325276118913	13.35391643033692
7	27.1067879440623	32.424271039805255	22.83577598097823	17.63316503515422
8	24.510575267940276	36.78549163122397	20.901510878515566	17.802422222320185
9	25.726117973303538	17.332005571218065	21.36826164400121	35.57361481147719
10-11	24.639670125978856	26.701521968979648	28.610508701953986	20.04829920308751
12-13	25.35212803499946	24.161852098220677	28.868153175550216	21.617866691229647
14-15	22.433688653550377	25.533983803746118	26.72248480223591	25.309842740467598
16-17	21.922877655243873	28.68324636909111	27.355940556040604	22.037935419624414
18-19	21.904304608180635	27.79271458597882	29.165008704158158	21.137972101682386
20-21	22.738216745387593	26.931350263610888	29.06487799977308	21.265554991228438
22-23	22.450091449460903	26.791901864548063	28.969971048255033	21.788035637735998
24-25	22.191021893279327	26.966969510451218	29.051155866991614	21.790852729277844
26-27	22.106814630565616	27.078143105362084	29.050280820584177	21.76476144348812
28-29	22.086821856951023	27.076994542134646	29.097383216991503	21.738800383922833
30-31	22.318440725823503	26.937161254404884	29.002211097030354	21.742186922741258
32-33	22.092197685774085	27.140753264831712	28.941932949867983	21.82511609952622
34-35	22.271790410831475	27.18270598035358	28.911311938659424	21.634191670155516
36-37	22.21106886583723	27.032365264440976	28.933273701217555	21.823292168504235
38-39	22.07327678996301	27.12805918751694	28.989715581613584	21.808948440906466
40-41	22.44525318619512	26.93149068292121	28.949629169404297	21.673626961479375
42-43	22.121538405844735	27.074479587919182	28.751380160179558	22.05260184605653
44-45	22.15995112815743	27.15571948032749	28.729354892398067	21.95497449911701
46-47	22.328599791772596	27.07528873392896	28.58888404639113	22.007227427907313
48-49	22.23294915863714	27.31739080408947	28.4419073285523	22.00775270872109
50-51	22.161785554571768	27.517459052221295	28.156949991668128	22.163805401538802
52-53	22.203393472010287	27.36999621915842	28.01450499745269	22.412105311378603
54-55	22.38784761201029	27.072784213807292	28.361433084205345	22.177935089977073
56-57	22.211773833366667	26.846578521683274	28.487251831694515	22.45439581325554
58-59	22.04131475390761	26.584179925159095	28.562673377508364	22.81183194342493
60-61	22.16984170272015	26.94300408397788	28.869591152465148	22.01756306083683
62-63	21.75932560738329	27.16376862945421	28.847614936245026	22.229290826917474
64-65	21.601680619902112	27.438379507907477	28.4996469999045	22.46029287228591
66-67	21.649544227454125	28.068947508413167	27.977162866549016	22.304345397583692
68-69	21.769013819904348	28.076885459668656	27.9632615005227	22.190839219904294
70-71	21.874825315240436	27.4974952892936	27.925338577339954	22.702340818126014
72-73	22.004621482869172	26.73911516050061	28.02691733513961	23.229346021490606
74-75	22.06624323781	26.929801755732875	28.26394257287072	22.740012433586404
76-77	22.347340468093485	27.016105414921874	27.411627159838904	23.224926957145737
78-79	22.59813109918526	26.919795037055327	26.95080531985394	23.53126854390547
80-81	22.365938093805894	27.349212881716362	27.16890583590021	23.115943188577535
82-83	22.40084621988199	27.11464092807753	27.17430921552304	23.310203636517436
84-85	22.457342790073216	27.54180588003785	27.100635566608887	22.90021576328005
86-87	22.951636536737674	27.47850087760289	27.09650870566006	22.473353879999376
88-89	22.6593680991557	27.627527709400358	27.443218196931817	22.26988599451213
90-91	22.650185197865664	27.26151710915252	27.258266004784016	22.8300316881978
92-93	22.667446404875527	26.795627920018756	27.495836128154753	23.041089546950964
94-95	21.90096756336049	27.24067072313404	28.190467674253473	22.667894039251998
96-97	22.08561045890536	27.136429118480855	28.30856173317432	22.469398689439465
98-99	23.24274994171122	29.02657496441253	28.61968431155706	19.110990782319195
100	29.93439267388726	40.258716120093666	29.80689120601907	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	749.0
1	682.5
2	841.5
3	1129.5
4	1417.5
5	2088.5
6	2712.5
7	3483.0
8	4398.5
9	4846.0
10	4855.5
11	4889.5
12	5401.5
13	6619.5
14	9358.0
15	12624.0
16	15137.5
17	16750.5
18	16862.5
19	15649.0
20	13958.0
21	12633.5
22	12484.0
23	14129.0
24	17377.5
25	21871.0
26	28227.5
27	35528.5
28	43263.5
29	52487.5
30	61765.0
31	70611.5
32	81502.0
33	93505.0
34	104886.5
35	115856.5
36	127891.0
37	138675.0
38	147023.5
39	154209.0
40	161525.0
41	167506.5
42	171138.0
43	174992.5
44	179530.0
45	183782.0
46	185415.5
47	186379.5
48	186630.0
49	184674.5
50	181086.5
51	175360.0
52	167535.5
53	157843.0
54	146077.5
55	134529.0
56	122062.0
57	108865.5
58	96059.0
59	81484.0
60	67066.5
61	53567.0
62	41639.0
63	31700.5
64	23262.0
65	17152.0
66	12774.0
67	9720.5
68	7570.0
69	5831.0
70	4332.5
71	3077.5
72	2198.5
73	1520.5
74	973.0
75	609.5
76	411.0
77	272.0
78	178.0
79	105.0
80	63.5
81	42.0
82	30.5
83	20.0
84	13.5
85	10.0
86	7.5
87	7.5
88	6.5
89	5.5
90	5.0
91	4.5
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009907171887576104
3	7.424578463757269E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.960631134346213E-5
22-23	1.1601579485437465E-5
24-25	3.3647047575997076E-4
26-27	3.7131628140668534E-4
28-29	0.00257675269964175
30-31	0.003310959380104759
32-33	0.002817065034972931
34-35	8.403515330521346E-4
36-37	1.171707665499021E-5
38-39	3.530967883022329E-5
40-41	4.022135943462964E-4
42-43	0.0028340708665374106
44-45	4.0798366433408004E-4
46-47	8.353238859412447E-4
48-49	0.0011481381596175869
50-51	0.0011823354647803818
52-53	0.0
54-55	0.00347206090662059
56-57	0.05243297147453483
58-59	0.06679968876293162
60-61	0.043498153103038444
62-63	0.00838951356017925
64-65	0.0
66-67	0.0
68-69	1.0322402167498007E-4
70-71	0.0015505576926033709
72-73	0.00849910088459063
74-75	0.01249310456303674
76-77	0.013789757788063885
78-79	0.004250028356080911
80-81	0.0072757619069826664
82-83	0.04426513044743288
84-85	0.037235301856366046
86-87	0.008409741777258763
88-89	0.0020492646057421247
90-91	0.002503000003740115
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	151.0
22-23	322.0
24-25	369.0
26-27	933.0
28-29	2425.0
30-31	8150.0
32-33	10547.0
34-35	14382.0
36-37	23995.0
38-39	15258.0
40-41	27448.0
42-43	30477.0
44-45	35970.0
46-47	37045.0
48-49	34648.0
50-51	17761.0
52-53	2860.0
54-55	2495.0
56-57	2213.0
58-59	18450.0
60-61	34595.0
62-63	33331.0
64-65	35263.0
66-67	36550.0
68-69	37325.0
70-71	37882.0
72-73	34568.0
74-75	34630.0
76-77	36352.0
78-79	38160.0
80-81	37446.0
82-83	35404.0
84-85	34372.0
86-87	35539.0
88-89	37197.0
90-91	38364.0
92-93	37309.0
94-95	43841.0
96-97	147625.0
98-99	1063845.0
100-101	2154485.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.94377445829411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.03826138059395	80.06248325930183
2	6.646608422451338	11.690556640333174
3	1.4205190849324183	3.7477743005699686
4	0.45788462190785045	1.6107240766794113
5	0.18334654946016027	0.8062093796715402
6	0.0924943837701587	0.48805831349650425
7	0.05109626633335066	0.3145518965456783
8	0.029648828535753954	0.20859439117607842
9	0.019366095206872203	0.15328147581099177
>10	0.05915092454971341	0.7668426825368412
>50	0.0011070184613412883	0.06808580753060067
>100	5.164237970408925E-4	0.08283777634734381
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.3201807699241464E-5	0.0
6	1.6241265389469026E-4	0.0	0.0	2.3201807699241464E-5	0.0
7	1.6241265389469026E-4	0.0	0.0	2.3201807699241464E-5	0.0
8	1.856144615939317E-4	0.0	0.0	2.3201807699241464E-5	0.0
9	2.552198846916561E-4	6.960542309772439E-5	0.0	4.640361539848293E-5	2.3201807699241464E-5
10-11	2.552198846916561E-4	6.960542309772439E-5	0.0	1.2760994234582804E-4	2.3201807699241464E-5
12-13	2.6682078854127684E-4	6.960542309772439E-5	0.0	1.3921084619544877E-4	2.3201807699241464E-5
14-15	2.7842169239089754E-4	1.1600903849620731E-4	0.0	1.3921084619544877E-4	3.48027115488622E-5
16-17	3.712289231878634E-4	1.1600903849620731E-4	0.0	1.3921084619544877E-4	5.800451924810366E-5
18-19	3.712289231878634E-4	1.2760994234582804E-4	0.0	1.6241265389469026E-4	6.960542309772439E-5
20-21	3.712289231878634E-4	1.3921084619544877E-4	0.0	1.6241265389469026E-4	6.960542309772439E-5
22-23	3.828298270374842E-4	1.3921084619544877E-4	0.0	1.9721536544355244E-4	1.0440813464658659E-4
24-25	4.060316347367256E-4	1.508117500450695E-4	0.0	2.3201807699241463E-4	1.3921084619544877E-4
26-27	4.1763253858634634E-4	1.6241265389469026E-4	0.0	3.3642621163900127E-4	1.3921084619544877E-4
28-29	4.1763253858634634E-4	1.6241265389469026E-4	0.0	6.148479040298988E-4	1.3921084619544877E-4
30-31	4.292334424359671E-4	1.6241265389469026E-4	0.0	0.001856144615939317	1.3921084619544877E-4
32-33	4.408343462855878E-4	1.6241265389469026E-4	0.0	0.004698366059096396	1.3921084619544877E-4
34-35	4.408343462855878E-4	2.0881626929317317E-4	0.0	0.008352650771726927	1.3921084619544877E-4
36-37	4.408343462855878E-4	2.552198846916561E-4	0.0	0.016995324139694373	1.3921084619544877E-4
38-39	4.524352501352085E-4	2.7842169239089754E-4	0.0	0.1843267612666238	1.508117500450695E-4
40-41	4.6403615398482926E-4	2.7842169239089754E-4	0.0	0.3575050539337621	1.6241265389469026E-4
42-43	4.6403615398482926E-4	2.7842169239089754E-4	0.0	0.37744700765126016	1.6241265389469026E-4
44-45	4.6403615398482926E-4	2.7842169239089754E-4	0.0	0.4041406874092375	1.6241265389469026E-4
46-47	4.6403615398482926E-4	2.7842169239089754E-4	0.0	0.43395501030276273	1.6241265389469026E-4
48-49	4.6403615398482926E-4	2.7842169239089754E-4	0.0	0.4640709566963781	1.6241265389469026E-4
50-51	4.872379616840707E-4	2.7842169239089754E-4	0.0	0.49223795124325725	1.6241265389469026E-4
52-53	4.988388655336914E-4	2.7842169239089754E-4	0.0	0.995775182836045	1.7401355774431098E-4
54-55	5.220406732329329E-4	2.7842169239089754E-4	0.0	1.680576537079157	1.856144615939317E-4
56-57	5.336415770825537E-4	2.7842169239089754E-4	0.0	2.422941576224087	1.856144615939317E-4
58-59	5.336415770825537E-4	2.9002259624051824E-4	0.0	3.1877891670295817	1.856144615939317E-4
60-61	5.684442886314158E-4	3.248253077893805E-4	0.0	3.3893664723205914	1.856144615939317E-4
62-63	6.264488078795195E-4	3.4802711548862197E-4	0.0	3.396593835418905	1.856144615939317E-4
64-65	6.264488078795195E-4	4.408343462855878E-4	0.0	3.403136745190091	1.856144615939317E-4
66-67	6.49650615578761E-4	4.408343462855878E-4	0.0	3.4087631835571575	1.856144615939317E-4
68-69	6.49650615578761E-4	4.524352501352085E-4	0.0	3.4147840526551105	1.856144615939317E-4
70-71	6.960542309772439E-4	4.6403615398482926E-4	0.0	3.4198884503489437	2.0881626929317317E-4
72-73	7.192560386764853E-4	4.6403615398482926E-4	0.0	3.4237515513308674	2.3201807699241463E-4
74-75	7.192560386764853E-4	4.6403615398482926E-4	0.0	3.4271042125434077	2.3201807699241463E-4
76-77	7.308569425261061E-4	4.872379616840707E-4	0.0	3.4303988692367	2.552198846916561E-4
78-79	7.424578463757269E-4	4.872379616840707E-4	0.0	3.432104202102594	2.7842169239089754E-4
80-81	7.424578463757269E-4	4.872379616840707E-4	0.0	3.432939467179767	2.7842169239089754E-4
82-83	7.424578463757269E-4	4.872379616840707E-4	0.0	3.433426705141451	3.01623500090139E-4
84-85	7.424578463757269E-4	4.872379616840707E-4	0.0	3.433728328641541	3.01623500090139E-4
86-87	7.424578463757269E-4	4.872379616840707E-4	0.0	3.4339603467185333	3.248253077893805E-4
88	7.424578463757269E-4	5.800451924810366E-4	0.0	3.4339603467185333	3.248253077893805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	4175	0.0	62.136063	94
GGTATCA	5990	0.0	44.86229	1
TATCTCG	5270	0.0	43.974724	94
GTATCAA	10425	0.0	35.40704	1
TATGCCG	2560	0.0	30.059294	86-87
TCAACGC	12260	0.0	29.4352	4
ATCAACG	12280	0.0	29.352705	3
TCTCGTA	2515	0.0	29.311079	82-83
GCCGTCT	2730	0.0	29.27179	90-91
CCGTCTT	2725	0.0	28.916782	90-91
CAACGCA	12505	0.0	28.8239	5
ATGCCGT	2750	0.0	28.502327	88-89
TATCAAC	12835	0.0	28.489017	2
TGCCGTC	2755	0.0	28.251642	88-89
CTCGTAT	2700	0.0	27.990208	82-83
AACGCAG	12930	0.0	27.876476	6
TCGTATG	2780	0.0	27.770786	84-85
CGTATGC	2935	0.0	26.578186	84-85
CTATCTC	6735	0.0	25.784369	92-93
ACGCAGA	14400	0.0	25.090853	7
>>END_MODULE