FastQCFastQC Report
Fri 27 May 2016
SRR2139485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057879
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.047.6111371
GTATCAA106000.036.3265271
CTTATAC67000.035.279731
TCAACGC124000.030.638534
ATCAACG125500.030.2391823
CAACGCA127300.029.7777425
TATCAAC131050.029.1888222
AACGCAG132650.028.6725466
TATACAC90850.028.1590333
TTATACA90950.027.014142
ACGCAGA152650.024.8326687
CGCAGAG157050.024.190888
ACATCTC112550.021.713388
TACACAT117850.021.7072355
ACACATC110950.021.6447666
GTGGTAT30300.021.2503851
TGGTATC28900.020.5208842
GCAGAGT184700.020.4547969
GAGTACT104050.019.49802412-13
CACATCT122750.019.4605337
Produced by FastQC (version 0.11.5)