##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139485_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5057879
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.632371592914737	32.0	18.0	33.0	18.0	33.0
2	29.042498644194534	31.0	27.0	33.0	18.0	33.0
3	31.005897135933857	33.0	30.0	33.0	27.0	33.0
4	32.236160453818684	33.0	32.0	33.0	31.0	33.0
5	32.84633202969071	33.0	33.0	33.0	32.0	34.0
6	36.64827094519264	38.0	37.0	38.0	34.0	38.0
7	37.18276712432227	38.0	38.0	38.0	36.0	38.0
8	37.38827045882276	38.0	38.0	38.0	37.0	38.0
9	37.360268602708764	38.0	38.0	38.0	37.0	38.0
10-11	37.411557888197805	38.0	38.0	38.0	37.0	38.0
12-13	37.463417768594304	38.0	38.0	38.0	37.0	38.0
14-15	37.49496775229301	38.0	38.0	38.0	37.0	38.0
16-17	37.52904972617969	38.0	38.0	38.0	37.5	38.0
18-19	37.524051979100335	38.0	38.0	38.0	37.0	38.0
20-21	37.53561011635773	38.0	38.0	38.0	37.5	38.0
22-23	37.54061914232048	38.0	38.0	38.0	38.0	38.0
24-25	37.5512953796011	38.0	38.0	38.0	38.0	38.0
26-27	37.521651070534105	38.0	38.0	38.0	37.5	38.0
28-29	37.49575089950507	38.0	38.0	38.0	37.0	38.0
30-31	37.49681275978474	38.0	38.0	38.0	37.0	38.0
32-33	37.47918765518297	38.0	38.0	38.0	37.0	38.0
34-35	37.375099639158904	38.0	38.0	38.0	37.0	38.0
36-37	37.4143294915879	38.0	38.0	38.0	37.0	38.0
38-39	37.40311867395785	38.0	38.0	38.0	37.0	38.0
40-41	37.43777091176479	38.0	38.0	38.0	37.0	38.0
42-43	37.414653773350935	38.0	38.0	38.0	37.0	38.0
44-45	37.38767488891042	38.0	38.0	38.0	37.0	38.0
46-47	37.3628042733922	38.0	38.0	38.0	37.0	38.0
48-49	37.306275298819486	38.0	38.0	38.0	37.0	38.0
50-51	37.272346009575074	38.0	38.0	38.0	37.0	38.0
52-53	37.24964280283118	38.0	38.0	38.0	37.0	38.0
54-55	37.21694454850775	38.0	38.0	38.0	37.0	38.0
56-57	37.16883000910866	38.0	38.0	38.0	37.0	38.0
58-59	37.152972724341055	38.0	38.0	38.0	36.0	38.0
60-61	37.147879343607144	38.0	38.0	38.0	36.0	38.0
62-63	37.15372238894552	38.0	38.0	38.0	36.0	38.0
64-65	37.15956037446804	38.0	38.0	38.0	36.0	38.0
66-67	37.15318797438087	38.0	38.0	38.0	36.0	38.0
68-69	37.13951082428834	38.0	38.0	38.0	36.0	38.0
70-71	37.135458151572905	38.0	38.0	38.0	36.0	38.0
72-73	37.125897397959164	38.0	38.0	38.0	36.0	38.0
74-75	37.12603478823996	38.0	38.0	38.0	36.0	38.0
76-77	37.115304160144234	38.0	38.0	38.0	36.0	38.0
78-79	37.10516357526812	38.0	38.0	38.0	36.0	38.0
80-81	37.09623212676226	38.0	38.0	38.0	36.0	38.0
82-83	37.04767055352542	38.0	38.0	38.0	36.0	38.0
84-85	37.04317292106519	38.0	38.0	38.0	36.0	38.0
86-87	37.03285283096001	38.0	38.0	38.0	36.0	38.0
88-89	37.01886974512621	38.0	38.0	38.0	36.0	38.0
90-91	37.01006137514942	38.0	38.0	38.0	36.0	38.0
92-93	36.992057044299656	38.0	38.0	38.0	36.0	38.0
94-95	36.97934641947889	38.0	38.0	38.0	36.0	38.0
96-97	36.96462386653188	38.0	38.0	38.0	35.0	38.0
98-99	36.97857769303137	38.0	38.0	38.0	35.0	38.0
100	35.60315010514656	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	12.0
20	24.0
21	84.0
22	230.0
23	727.0
24	1697.0
25	3398.0
26	6472.0
27	10962.0
28	17561.0
29	25368.0
30	34187.0
31	44522.0
32	57275.0
33	77988.0
34	119702.0
35	228150.0
36	760597.0
37	3668919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97226900841242	21.385031156340435	14.361968722462517	24.28073111278463
2	14.089465650062483	21.1933696632235	42.32538320388503	22.391781482828986
3	18.457557738131484	26.119688341021735	30.35217037484015	25.07058354600663
4	12.799891021513169	18.408309095571486	38.422666892584814	30.369132990330534
5	13.54743757215228	37.75456075560527	35.35549189689986	13.34250977534259
6	29.806406993919783	38.014313905097374	18.608748845118676	13.570530255864169
7	26.526198036766	32.62907238389847	22.652044463697134	18.192685115638394
8	23.978529340065272	37.59761749935101	21.013828128351825	17.41002503223189
9	25.029859354088934	17.667346332326257	21.2113417501684	36.091452563416404
10-11	24.266970403997405	27.070764247226954	28.661272837883235	20.000992510892413
12-13	25.13275821742671	24.159474752163902	28.855366053636317	21.85240097677307
14-15	22.18848058642763	25.798521475108437	26.18133015835294	25.83166778011099
16-17	22.024637204646453	29.162411754017842	26.82316045915689	21.989790582178813
18-19	22.09940174527702	28.038294312695104	28.52591965920893	21.336384282818944
20-21	22.635669229564698	27.192438396335493	28.465586727551973	21.70630564654784
22-23	22.36668266888536	27.462881838773562	28.336472483565434	21.833963008775648
24-25	22.460171725833057	27.475263260247974	28.475825713910304	21.588739300008672
26-27	22.48523974731966	27.587691404723596	28.452977600169593	21.47409124778715
28-29	22.431344885468565	27.45309452823319	28.437537987322013	21.67802259897623
30-31	22.309711416018065	27.644141997235362	28.59735959547037	21.448786991276204
32-33	22.341830080598633	27.738669336255594	28.432987348390782	21.48651323475499
34-35	22.584533481157095	27.469870744076896	28.544942406733092	21.40065336803292
36-37	22.089873883579372	27.681417478079172	28.47408561499187	21.754623023349577
38-39	22.04504207019221	27.32235954948883	28.746016261143286	21.886582119175674
40-41	22.286892469518477	27.201755923549964	28.695632901220797	21.81571870571076
42-43	22.37467498649609	27.214012317116378	28.57642735150206	21.834885344885475
44-45	22.123401444624786	27.500549742927777	28.51120371464968	21.86484509779775
46-47	22.52049858375494	27.23720138410972	28.0715698843076	22.170730147827737
48-49	22.32928630474065	27.266638185020703	28.10274806060275	22.301327449635895
50-51	22.0953702455818	27.453260704275763	28.333642316866094	22.117726733276346
52-53	22.267858560437258	27.47744688864354	28.469444783626262	21.78524976729293
54-55	22.24054083837101	27.49175689046209	28.148132124069065	22.119570147097832
56-57	22.48348023807816	27.795936216347954	27.738108407318645	21.98247513825524
58-59	22.035702998136383	28.11370249681223	28.23100144948069	21.619593055570693
60-61	22.352539043281425	28.578530898034227	27.54147027615847	21.527459782525877
62-63	22.00171858183612	28.96064398790848	27.535509944847654	21.502127485407748
64-65	21.993440753526166	28.904746478931592	27.514022295704915	21.58779047183733
66-67	22.091735748018763	28.9848644723003	27.331871408761383	21.591528370919562
68-69	22.193185742609494	28.546449525981256	27.57202924785785	21.688335483551406
70-71	22.27160598843144	28.11561634844758	27.696692957159886	21.9160847059611
72-73	22.375203056128054	27.795321072944866	27.812304538403904	22.017171332523173
74-75	22.23957825437212	28.013790841992197	27.719718615576827	22.026912288058856
76-77	22.41460957954276	27.69205479109416	27.74666165773027	22.14667397163281
78-79	22.355365037561235	27.67980324714623	27.758049491235386	22.206782224057147
80-81	22.278587984429947	27.763457052769407	27.74459021749678	22.213364745303863
82-83	22.423021827197196	27.691326723878618	27.80891682723321	22.076734621690978
84-85	22.426920609813823	27.694174260944926	27.774515266108253	22.104389863133
86-87	22.37626509802431	27.677186580378667	27.85256282330283	22.093985498294195
88-89	22.446037342873307	27.677553578476456	27.796598361904852	22.079810716745385
90-91	22.474089707684865	27.712201227365078	27.765029420083987	22.048679644866066
92-93	22.49198926916383	27.699688006217084	27.777715923628783	22.030606800990302
94-95	22.3260959027347	27.7820720496078	27.81952805172347	22.07230399593403
96-97	22.49082659391927	27.855534351324863	27.855305512551066	21.798333542204805
98-99	23.493345262060064	29.765463573939765	28.074313602717282	18.666877561282888
100	29.84090068401466	40.80209761769977	29.35700169828557	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	921.0
1	821.5
2	835.0
3	1006.0
4	1284.0
5	1802.0
6	2277.0
7	2953.0
8	3813.5
9	4387.0
10	4631.0
11	4786.0
12	5322.0
13	6682.0
14	9364.5
15	12512.0
16	14947.5
17	16275.5
18	16230.0
19	15303.0
20	14306.5
21	13906.5
22	14689.0
23	17330.5
24	22028.5
25	28642.0
26	37210.0
27	47076.0
28	57987.5
29	70164.0
30	82256.0
31	93977.0
32	106612.5
33	120669.0
34	133735.0
35	145670.0
36	158319.0
37	169445.5
38	179347.0
39	187818.0
40	195547.0
41	203448.0
42	211283.5
43	225577.5
44	239293.0
45	242412.5
46	236635.0
47	227196.5
48	219498.0
49	213191.0
50	207878.5
51	201277.0
52	191693.0
53	180456.5
54	167280.0
55	153513.0
56	139307.5
57	124739.5
58	109948.0
59	93398.5
60	77153.5
61	62153.5
62	49109.0
63	37993.5
64	28407.0
65	20993.0
66	15577.5
67	12021.0
68	9184.5
69	6760.5
70	5034.5
71	3654.5
72	2596.0
73	1780.5
74	1184.5
75	793.0
76	541.0
77	350.0
78	207.0
79	137.5
80	105.0
81	75.0
82	49.0
83	34.5
84	24.5
85	16.0
86	10.0
87	5.0
88	4.0
89	5.0
90	4.5
91	4.5
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010261218190470748
3	8.501587325438193E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.0874246946041887E-4
22-23	0.0
24-25	1.779600616097733E-4
26-27	3.5595337959936263E-4
28-29	0.002383655364183966
30-31	0.0030888844315670526
32-33	0.002311694156314973
34-35	6.466584570888392E-4
36-37	9.989893225024243E-6
38-39	2.0104024252690696E-5
40-41	1.9228653689619372E-4
42-43	0.0026186866216700902
44-45	3.081221301633181E-4
46-47	5.595932047560863E-4
48-49	0.0010664819770819297
50-51	8.33088591589918E-4
52-53	0.0
54-55	0.003330438386354412
56-57	0.0545247910728773
58-59	0.07121356784548953
60-61	0.04657220136290174
62-63	0.007785920460500595
64-65	0.0
66-67	0.0
68-69	1.2612354580984904E-4
70-71	0.0011388079005379124
72-73	0.007931273920510016
74-75	0.012500495555359516
76-77	0.014440294312676258
78-79	0.004155140303415149
80-81	0.008032499443620451
82-83	0.04788000330206919
84-85	0.040568037803642674
86-87	0.009578190915389986
88-89	0.002074532577076569
90-91	0.0027196907944683044
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	146.0
22-23	316.0
24-25	366.0
26-27	1160.0
28-29	3080.0
30-31	10235.0
32-33	12799.0
34-35	17988.0
36-37	29268.0
38-39	33774.0
40-41	32974.0
42-43	37137.0
44-45	42711.0
46-47	43293.0
48-49	41421.0
50-51	37619.0
52-53	35203.0
54-55	38178.0
56-57	38911.0
58-59	40766.0
60-61	41960.0
62-63	42223.0
64-65	47823.0
66-67	53631.0
68-69	55167.0
70-71	65143.0
72-73	44602.0
74-75	40907.0
76-77	42552.0
78-79	44395.0
80-81	43963.0
82-83	40931.0
84-85	40454.0
86-87	41508.0
88-89	43344.0
90-91	44050.0
92-93	42637.0
94-95	49131.0
96-97	150558.0
98-99	1138936.0
100-101	2446585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.17011410990688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.25646082447125	80.46092564159876
2	6.496426937428924	11.455814087595622
3	1.348176709700088	3.566066830037267
4	0.45099829622668575	1.5905828496672183
5	0.1988753517340142	0.8767431228017949
6	0.0901535955822015	0.4769311685940661
7	0.04861450216921714	0.3000442342559333
8	0.03164412414611315	0.2232052829496685
9	0.018170153075119145	0.14418580230249572
>10	0.05904604715096338	0.7786330047200406
>50	0.001017165743251384	0.05918465553351566
>100	4.1629257217490025E-4	0.06768331994365724
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.977113331497254E-5	0.0	0.0	0.0	0.0
6	1.977113331497254E-5	0.0	0.0	0.0	0.0
7	1.977113331497254E-5	0.0	0.0	0.0	0.0
8	1.977113331497254E-5	0.0	0.0	0.0	0.0
9	1.977113331497254E-5	9.88556665748627E-5	0.0	0.0	0.0
10-11	2.965669997245881E-5	9.88556665748627E-5	0.0	0.0	0.0
12-13	3.954226662994508E-5	9.88556665748627E-5	0.0	0.0	0.0
14-15	5.9313399944917624E-5	1.5816906651978032E-4	0.0	0.0	0.0
16-17	5.9313399944917624E-5	1.5816906651978032E-4	0.0	0.0	0.0
18-19	7.908453325989016E-5	1.5816906651978032E-4	0.0	0.0	0.0
20-21	1.0874123323234897E-4	1.8782576649223914E-4	0.0	0.0	0.0
22-23	1.1862679988983525E-4	2.1748246646469794E-4	0.0	0.0	0.0
24-25	1.2851236654732153E-4	2.4713916643715676E-4	0.0	1.977113331497254E-5	0.0
26-27	1.383979332048078E-4	2.5702473309464305E-4	0.0	1.0874123323234897E-4	0.0
28-29	1.383979332048078E-4	2.5702473309464305E-4	0.0	4.4485049958688217E-4	0.0
30-31	1.4828349986229406E-4	3.262236996970469E-4	0.0	0.0017596308650325563	0.0
32-33	1.5816906651978032E-4	3.361092663545332E-4	0.0	0.004853813228825759	0.0
34-35	1.680546331772666E-4	3.855370996419645E-4	0.0	0.009420945024584416	0.0
36-37	1.977113331497254E-4	4.349649329293959E-4	0.0	0.016232100451592457	0.0
38-39	1.977113331497254E-4	4.349649329293959E-4	0.0	0.03318584726918141	0.0
40-41	1.977113331497254E-4	4.74507199559341E-4	0.0	0.05218590638487002	0.0
42-43	1.977113331497254E-4	5.041638995317998E-4	0.0	0.0645033224400979	0.0
44-45	2.0759689980721167E-4	5.535917328192311E-4	0.0	0.07918338892646502	0.0
46-47	2.1748246646469794E-4	5.733628661342036E-4	0.0	0.09443681827896634	0.0
48-49	2.2736803312218423E-4	5.733628661342036E-4	0.0	0.1094431084650305	0.0
50-51	2.372535997796705E-4	6.919896660240389E-4	0.0	0.12462733885092941	0.0
52-53	2.372535997796705E-4	6.919896660240389E-4	0.0	0.158772086085887	0.0
54-55	2.372535997796705E-4	6.919896660240389E-4	0.0	0.1998663866810574	0.0
56-57	2.372535997796705E-4	7.117607993390115E-4	0.0	0.2577958072939269	0.0
58-59	2.372535997796705E-4	7.117607993390115E-4	0.0	0.2964187162247258	0.0
60-61	2.372535997796705E-4	7.513030659689565E-4	0.0	0.31445987537463826	0.0
62-63	2.372535997796705E-4	7.908453325989016E-4	0.0	0.32859623569484364	0.0
64-65	2.372535997796705E-4	8.303875992288467E-4	0.0	0.33494276948894985	0.0
66-67	2.372535997796705E-4	8.303875992288467E-4	0.0	0.3410322785499614	0.0
68-69	2.372535997796705E-4	8.501587325438192E-4	0.0	0.3478236628436544	0.0
70-71	2.372535997796705E-4	8.897009991737643E-4	0.0	0.35371546057151626	0.0
72-73	2.372535997796705E-4	8.897009991737643E-4	0.0	0.3584308758671372	0.0
74-75	2.372535997796705E-4	9.094721324887369E-4	0.0	0.36170299843076514	0.0
76-77	2.372535997796705E-4	9.687855324336545E-4	0.0	0.36480706636121585	0.0
78-79	2.372535997796705E-4	0.0010182133657210858	0.0	0.3666260106261933	0.0
80-81	2.372535997796705E-4	0.0010280989323785722	0.0	0.3672883435922449	0.0
82-83	2.372535997796705E-4	0.0010280989323785722	0.0	0.36791113429166655	0.0
84-85	2.372535997796705E-4	0.0010280989323785722	0.0	0.36819781572473365	0.0
86-87	2.372535997796705E-4	0.0010280989323785722	0.0	0.3683856414912259	0.0
88	2.372535997796705E-4	0.0010478700656935446	0.0	0.36839552705788337	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5890	0.0	47.611137	1
GTATCAA	10600	0.0	36.326527	1
CTTATAC	6700	0.0	35.27973	1
TCAACGC	12400	0.0	30.63853	4
ATCAACG	12550	0.0	30.239182	3
CAACGCA	12730	0.0	29.777742	5
TATCAAC	13105	0.0	29.188822	2
AACGCAG	13265	0.0	28.672546	6
TATACAC	9085	0.0	28.159033	3
TTATACA	9095	0.0	27.01414	2
ACGCAGA	15265	0.0	24.832668	7
CGCAGAG	15705	0.0	24.19088	8
ACATCTC	11255	0.0	21.71338	8
TACACAT	11785	0.0	21.707235	5
ACACATC	11095	0.0	21.644766	6
GTGGTAT	3030	0.0	21.250385	1
TGGTATC	2890	0.0	20.520884	2
GCAGAGT	18470	0.0	20.454796	9
GAGTACT	10405	0.0	19.498024	12-13
CACATCT	12275	0.0	19.460533	7
>>END_MODULE