FastQCFastQC Report
Fri 27 May 2016
SRR2139484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6309001
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84150.043.9569971
GTATCAA144350.035.190211
TCAACGC167600.029.4918214
ATCAACG168650.029.2829443
CAACGCA171400.028.813125
AACGCAG178050.027.784846
TATCAAC180200.027.6453532
ACGCAGA205500.024.0319637
CGCAGAG212350.023.256748
GCAGAGT246900.019.9850449
GAGTACT135800.019.21869712-13
GTGGTAT47450.018.8601651
GTACATG190650.018.7313731
TGGTATC44550.018.6530272
TACATGG193650.018.0230712
AGAGTAC218450.017.94541210-11
CAGAGTA239350.017.74922410-11
ACATGGG189800.017.6234573
GTACTTT152650.017.12532614-15
TATACCG4251.8189894E-1217.044245
Produced by FastQC (version 0.11.5)