##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6309001
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.54093667127331	32.0	18.0	33.0	18.0	33.0
2	28.957972427013406	31.0	27.0	33.0	18.0	33.0
3	31.05021381356573	33.0	30.0	33.0	27.0	33.0
4	32.282519847437015	33.0	32.0	33.0	31.0	33.0
5	32.868718359689595	33.0	33.0	33.0	32.0	34.0
6	36.735370465149714	38.0	37.0	38.0	34.0	38.0
7	37.249876327488295	38.0	38.0	38.0	36.0	38.0
8	37.42559194395436	38.0	38.0	38.0	37.0	38.0
9	37.378911019351555	38.0	38.0	38.0	37.0	38.0
10-11	37.44963743071209	38.0	38.0	38.0	37.0	38.0
12-13	37.49591480489542	38.0	38.0	38.0	37.0	38.0
14-15	37.527087648266345	38.0	38.0	38.0	37.0	38.0
16-17	37.5581615060768	38.0	38.0	38.0	38.0	38.0
18-19	37.554412655823	38.0	38.0	38.0	38.0	38.0
20-21	37.56295372670586	38.0	38.0	38.0	38.0	38.0
22-23	37.56791489272623	38.0	38.0	38.0	38.0	38.0
24-25	37.57781145956753	38.0	38.0	38.0	38.0	38.0
26-27	37.55060497462828	38.0	38.0	38.0	38.0	38.0
28-29	37.52495647501344	38.0	38.0	38.0	38.0	38.0
30-31	37.527409992822015	38.0	38.0	38.0	38.0	38.0
32-33	37.517001918443	38.0	38.0	38.0	38.0	38.0
34-35	37.413830881448774	38.0	38.0	38.0	37.0	38.0
36-37	37.45929566006402	38.0	38.0	38.0	37.0	38.0
38-39	37.463878372318774	38.0	38.0	38.0	37.0	38.0
40-41	37.46029667245044	38.0	38.0	38.0	38.0	38.0
42-43	37.42853764340425	38.0	38.0	38.0	37.0	38.0
44-45	37.39942168490806	38.0	38.0	38.0	37.0	38.0
46-47	37.369698059366954	38.0	38.0	38.0	37.0	38.0
48-49	37.31107421207124	38.0	38.0	38.0	37.0	38.0
50-51	37.27637316917817	38.0	38.0	38.0	37.0	38.0
52-53	37.2532598994962	38.0	38.0	38.0	37.0	38.0
54-55	37.22573897965643	38.0	38.0	38.0	37.0	38.0
56-57	37.18094095927056	38.0	38.0	38.0	37.0	38.0
58-59	37.17382229529004	38.0	38.0	38.0	37.0	38.0
60-61	37.17202424544109	38.0	38.0	38.0	36.5	38.0
62-63	37.178808239988854	38.0	38.0	38.0	36.0	38.0
64-65	37.18487381986086	38.0	38.0	38.0	36.0	38.0
66-67	37.182207340321746	38.0	38.0	38.0	36.0	38.0
68-69	37.174763790922555	38.0	38.0	38.0	36.0	38.0
70-71	37.168792019585055	38.0	38.0	38.0	36.0	38.0
72-73	37.161070342163725	38.0	38.0	38.0	36.0	38.0
74-75	37.15886923607768	38.0	38.0	38.0	36.0	38.0
76-77	37.14596428759411	38.0	38.0	38.0	36.0	38.0
78-79	37.13359434541788	38.0	38.0	38.0	36.0	38.0
80-81	37.12345067837505	38.0	38.0	38.0	36.0	38.0
82-83	37.076360464024255	38.0	38.0	38.0	36.0	38.0
84-85	37.06846058874379	38.0	38.0	38.0	36.0	38.0
86-87	37.0579431945635	38.0	38.0	38.0	36.0	38.0
88-89	37.04288394918905	38.0	38.0	38.0	36.0	38.0
90-91	37.03235840725564	38.0	38.0	38.0	36.0	38.0
92-93	37.01213910426249	38.0	38.0	38.0	36.0	38.0
94-95	36.99703765476523	38.0	38.0	38.0	36.0	38.0
96-97	36.98317139876215	38.0	38.0	38.0	36.0	38.0
98-99	36.99375551405535	38.0	38.0	38.0	35.0	38.0
100	35.617803664541945	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	24.0
21	67.0
22	248.0
23	776.0
24	1993.0
25	4013.0
26	7500.0
27	12859.0
28	20767.0
29	30770.0
30	41446.0
31	53556.0
32	69023.0
33	93032.0
34	140284.0
35	266957.0
36	911683.0
37	4653999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67222370070951	21.28321108207147	14.375445494461008	23.66911972275801
2	14.345276422710098	21.536064984913807	41.883686211027864	22.234972381348225
3	18.679929066008302	26.181366702995927	30.312457005619308	24.826247225376463
4	12.996257252138651	17.999410683244463	39.12292928785398	29.881402776762915
5	13.685621542935245	37.841458576405365	35.244232803259976	13.228687077399417
6	30.116590566398706	38.093273404141165	18.71969904585528	13.070436983604852
7	26.702658630106413	32.88910558105792	23.07252764740408	17.33570814143158
8	24.25304735250478	37.4090446332153	21.007161038649382	17.330746975630532
9	25.63420104070359	17.56331945422104	21.611091835300073	35.191387669775295
10-11	24.375697515343557	27.101604517101833	28.841333199978887	19.681364767575722
12-13	25.23925737212595	24.481617612677507	29.11979249963663	21.15933251555991
14-15	22.298078570600953	26.01875479176497	26.750780353339614	24.932386284294456
16-17	21.82925632758657	29.152539363997565	27.438718110838785	21.57948619757708
18-19	21.834360146717366	28.230198410176193	29.205574701921904	20.729866741184537
20-21	22.544370937031292	27.406353338841438	29.159340691999976	20.889935032127298
22-23	22.36763974385353	27.268617428523513	29.007868334195123	21.355874493427837
24-25	22.15893269889336	27.369442206813726	29.063009188689747	21.408615905603167
26-27	22.049324476705774	27.549325071234442	29.036098591878662	21.365251860181125
28-29	22.044482646788055	27.50041431393181	29.120980461034268	21.33412257824586
30-31	22.24725430797513	27.41983244375777	29.01550489296323	21.31740835530387
32-33	22.051235926891888	27.597948323505655	28.93429119377238	21.416524555830073
34-35	22.209846086675242	27.60702210059768	28.906641246066535	21.27649056666055
36-37	22.177860121464228	27.45855724725476	28.907428669861655	21.456153961419353
38-39	22.05165856999691	27.626577409393636	29.017175106180478	21.304588914428972
40-41	22.253071197528214	27.509504857741305	28.879794085925713	21.357629858804764
42-43	22.145760225739885	27.622932064552185	28.713387355689544	21.517920354018383
44-45	22.19086194106584	27.69252497258543	28.623309948274844	21.493303138073887
46-47	22.289627020552217	27.67016185134437	28.392826881667414	21.647384246436
48-49	22.246158817279046	27.750376761077522	28.361452262150323	21.642012159493113
50-51	22.206799762689045	27.92124653468625	28.269604253396935	21.602349449227766
52-53	22.360357096903748	27.8770618947823	28.157036880616772	21.60554412769718
54-55	22.351724838397924	27.81540019383357	28.15312989243399	21.679745075334505
56-57	22.284965880585542	27.902404483613207	28.166423813479785	21.646205822321466
58-59	22.33064812650071	27.844939586541383	28.21803358976301	21.6063786971949
60-61	22.341944701424023	27.89571951904645	28.24125671312873	21.521079066400794
62-63	22.337237313889794	27.98792745157794	28.23254787054642	21.44228736398585
64-65	22.3362576774059	27.94037404152194	28.201978500143944	21.521389780928214
66-67	22.352830400614852	28.132135874194304	28.07132342089745	21.443710304293393
68-69	22.424528180256015	27.930434109937618	28.170777038284356	21.47426067152201
70-71	22.423001208005797	27.76069398754616	28.152587464415735	21.66371734003231
72-73	22.43636863346025	27.758380916451692	28.156704909103603	21.64854554098445
74-75	22.254774362352773	27.97651273200683	28.068451782286534	21.700261123353865
76-77	22.403433881454454	27.677656458960758	28.036098037013513	21.88281162257128
78-79	22.29993023344055	27.709202912768156	28.042591350052522	21.948275503738774
80-81	22.266848369849797	27.84348862404157	28.020528798653892	21.869134207454742
82-83	22.34257318946532	27.726950204733203	28.052996322924333	21.877480282877144
84-85	22.31418370020449	27.764869906756477	28.002679836629103	21.918266556409932
86-87	22.27939879587566	27.82056751787993	28.048704430217832	21.85132925602658
88-89	22.354214946744356	27.82077498537611	27.974645702463025	21.85036436541651
90-91	22.3584298659389	27.82270924197573	27.986745587299534	21.83211530478584
92-93	22.421919718876715	27.789534347483702	27.947114112747727	21.84143182089186
94-95	22.253892482309634	27.828416405233934	28.049982527329654	21.867708585126778
96-97	22.38818495383766	27.877989261099955	28.029370409819204	21.704455375243178
98-99	23.384588668764007	29.82551170177925	28.300380602842313	18.48951902661443
100	29.675971138665364	40.75062411281984	29.573404748514793	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1262.0
1	1115.0
2	1105.0
3	1380.0
4	1805.5
5	2456.5
6	3039.5
7	4020.0
8	5345.0
9	6167.0
10	6407.5
11	6568.5
12	7454.5
13	9518.5
14	13266.0
15	17471.0
16	20581.5
17	22328.0
18	22377.5
19	21140.0
20	19683.5
21	18928.0
22	19922.5
23	23369.0
24	29505.5
25	38124.5
26	48817.5
27	61212.5
28	74546.0
29	90108.0
30	105353.0
31	120073.0
32	137698.5
33	156059.5
34	172304.0
35	187684.5
36	205741.0
37	221534.5
38	234313.0
39	244570.5
40	252986.5
41	260164.0
42	262917.5
43	266874.5
44	272563.5
45	275639.0
46	274986.0
47	271624.5
48	267173.5
49	261182.5
50	253707.5
51	244912.0
52	232458.5
53	217481.5
54	201136.5
55	183819.5
56	166289.5
57	148607.5
58	130621.0
59	110697.0
60	91383.5
61	73679.0
62	58139.5
63	44880.5
64	33361.5
65	24918.5
66	18661.0
67	14345.5
68	11097.0
69	8333.0
70	6167.5
71	4373.5
72	3071.5
73	2092.5
74	1393.0
75	944.5
76	623.0
77	382.0
78	236.0
79	155.5
80	111.0
81	79.0
82	56.5
83	38.5
84	22.0
85	12.0
86	6.0
87	4.5
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009240765693332431
3	5.864636889421955E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.5477262168580815E-5
22-23	7.925715753556843E-6
24-25	7.133640027012717E-5
26-27	1.0305153020424497E-4
28-29	0.001474856550377135
30-31	0.0020714137756158886
32-33	0.0016143275947830976
34-35	4.3852256485629135E-4
36-37	0.0
38-39	2.4154336548812293E-5
40-41	1.0533258358140507E-4
42-43	0.0020467125026980316
44-45	1.7253465235253463E-4
46-47	4.5576547468802853E-4
48-49	8.274424722710654E-4
50-51	8.425967720286183E-4
52-53	0.0
54-55	0.003017871699416559
56-57	0.04884560996095453
58-59	0.06399326302560496
60-61	0.042328179578046764
62-63	0.007229289191187479
64-65	0.0
66-67	0.0
68-69	8.171525773673251E-5
70-71	0.0011921269369426291
72-73	0.006990900625227262
74-75	0.01146769805541674
76-77	0.012437590285770149
78-79	0.004042064160142006
80-81	0.007119251998785391
82-83	0.042626862126582996
84-85	0.035806188827407284
86-87	0.008679016025991767
88-89	0.002277953245962955
90-91	0.0024859523399305434
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	50.0
20-21	266.0
22-23	455.0
24-25	488.0
26-27	1349.0
28-29	3510.0
30-31	12015.0
32-33	15088.0
34-35	21373.0
36-37	34510.0
38-39	39429.0
40-41	38380.0
42-43	43874.0
44-45	51155.0
46-47	52141.0
48-49	49216.0
50-51	44362.0
52-53	41890.0
54-55	45425.0
56-57	47115.0
58-59	49031.0
60-61	48505.0
62-63	47367.0
64-65	49536.0
66-67	51963.0
68-69	54666.0
70-71	53258.0
72-73	50208.0
74-75	50956.0
76-77	52782.0
78-79	54659.0
80-81	54635.0
82-83	51085.0
84-85	50384.0
86-87	51926.0
88-89	54184.0
90-91	55912.0
92-93	54026.0
94-95	61531.0
96-97	190122.0
98-99	1441719.0
100-101	3138455.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.89941162088174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.20667819472298	80.17013349211254
2	6.437089351202846	11.316327330435472
3	1.4018469870699817	3.696645760378716
4	0.4657199334507185	1.6374603252173745
5	0.20284194331065417	0.8914843734521375
6	0.1055206479662969	0.5565121722055014
7	0.05861404137317334	0.36064978245967694
8	0.03396337816834519	0.23882887661240337
9	0.020901515132676855	0.16535077939325266
>10	0.0657787558085799	0.875010807063116
>50	7.79924062788452E-4	0.04338944643180978
>100	2.653277309339263E-4	0.04820685423791769
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5850369971410688E-5	0.0	4.7551109914232066E-5	0.0
3	0.0	1.5850369971410688E-5	0.0	4.7551109914232066E-5	0.0
4	0.0	4.7551109914232066E-5	0.0	4.7551109914232066E-5	0.0
5	0.0	4.7551109914232066E-5	0.0	4.7551109914232066E-5	0.0
6	1.426533297426962E-4	4.7551109914232066E-5	0.0	6.340147988564275E-5	0.0
7	1.426533297426962E-4	4.7551109914232066E-5	0.0	9.510221982846413E-5	0.0
8	1.426533297426962E-4	6.340147988564275E-5	0.0	9.510221982846413E-5	0.0
9	1.426533297426962E-4	1.1095258979987481E-4	0.0	1.1095258979987481E-4	0.0
10-11	1.5057851472840154E-4	1.1095258979987481E-4	0.0	1.268029597712855E-4	0.0
12-13	1.5850369971410688E-4	1.1095258979987481E-4	0.0	1.268029597712855E-4	7.925184985705344E-6
14-15	1.5850369971410688E-4	1.426533297426962E-4	0.0	1.3472814475699085E-4	4.755110991423206E-5
16-17	2.2190517959974962E-4	1.426533297426962E-4	0.0	1.5057851472840154E-4	6.340147988564275E-5
18-19	2.2983036458545497E-4	1.5850369971410688E-4	0.0	1.9020443965692824E-4	6.340147988564275E-5
20-21	2.377555495711603E-4	1.9020443965692827E-4	0.0	2.53605919542571E-4	6.340147988564275E-5
22-23	2.377555495711603E-4	1.9812962464263358E-4	0.0	3.3285776939962446E-4	7.925184985705344E-5
24-25	2.377555495711603E-4	2.2190517959974962E-4	0.0	3.6455850934244584E-4	9.510221982846413E-5
26-27	2.377555495711603E-4	2.377555495711603E-4	0.0	4.358851742137939E-4	9.510221982846413E-5
28-29	2.53605919542571E-4	2.4568073455686566E-4	0.0	8.400696084847664E-4	9.510221982846413E-5
30-31	2.53605919542571E-4	2.853066594853924E-4	0.0	0.0020605480962833894	9.510221982846413E-5
32-33	2.53605919542571E-4	2.853066594853924E-4	0.0	0.004660008771594742	1.1095258979987481E-4
34-35	2.6153110452827635E-4	3.5663332435674044E-4	0.0	0.008250117570119262	1.1095258979987481E-4
36-37	2.694562895139817E-4	4.2795998922808855E-4	0.0	0.014257407789283912	1.1095258979987481E-4
38-39	2.853066594853924E-4	4.4381035919949924E-4	0.0	0.028641618538339114	1.1095258979987481E-4
40-41	2.9323184447109773E-4	5.07211839085142E-4	0.0	0.04414328037037876	1.1095258979987481E-4
42-43	3.011570294568031E-4	5.151370240708474E-4	0.0	0.05342367198863972	1.1095258979987481E-4
44-45	3.1700739942821377E-4	5.785385039564901E-4	0.0	0.06546995316691184	1.1887777478558016E-4
46-47	3.3285776939962446E-4	6.340147988564275E-4	0.0	0.07793626914942635	1.268029597712855E-4
48-49	3.3285776939962446E-4	6.498651688278382E-4	0.0	0.08983197181297006	1.426533297426962E-4
50-51	3.3285776939962446E-4	7.608177586277131E-4	0.0	0.10185447743628508	1.5057851472840154E-4
52-53	3.3285776939962446E-4	7.608177586277131E-4	0.0	0.13261212036580752	1.6642888469981223E-4
54-55	3.3285776939962446E-4	7.608177586277131E-4	0.0	0.1718972623399489	1.8227925467122292E-4
56-57	3.3285776939962446E-4	7.608177586277131E-4	0.0	0.22946580607611256	1.9812962464263358E-4
58-59	3.3285776939962446E-4	7.608177586277131E-4	0.0	0.26693608068852737	2.0605480962833893E-4
60-61	3.4870813937103515E-4	7.84593313584829E-4	0.0	0.2804802218290978	2.0605480962833893E-4
62-63	3.883340642995618E-4	7.925184985705343E-4	0.0	0.2905689823159007	2.0605480962833893E-4
64-65	3.9625924928526717E-4	9.827229382274626E-4	0.0	0.2962751155056086	2.0605480962833893E-4
66-67	3.9625924928526717E-4	9.90648123213168E-4	0.0	0.30188614647548795	2.0605480962833893E-4
68-69	3.9625924928526717E-4	0.0010461244181131054	0.0	0.3075843544802101	2.0605480962833893E-4
70-71	4.1210961925667786E-4	0.0010936755280273375	0.0	0.31250589435633314	2.0605480962833893E-4
72-73	4.2795998922808855E-4	0.0011095258979987482	0.0	0.31662699054889987	2.853066594853924E-4
74-75	4.2795998922808855E-4	0.0011491518229272749	0.0	0.31968611195338215	2.853066594853924E-4
76-77	4.358851742137939E-4	0.0012284036727843283	0.0	0.32307809112726404	2.853066594853924E-4
78-79	4.4381035919949924E-4	0.001268029597712855	0.0	0.32532091847821865	2.853066594853924E-4
80-81	4.4381035919949924E-4	0.001268029597712855	0.0	0.3260421103119179	2.853066594853924E-4
82-83	4.517355441852046E-4	0.001291805152669971	0.0	0.32647007030114594	2.853066594853924E-4
84-85	4.5966072917090993E-4	0.0013155807076270871	0.0	0.32662857400086004	3.090822144425084E-4
86-87	4.755110991423206E-4	0.0013155807076270871	0.0	0.32676330214561705	3.1700739942821377E-4
88	4.755110991423206E-4	0.0013631318175413192	0.0	0.32677122733060276	3.3285776939962446E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8415	0.0	43.956997	1
GTATCAA	14435	0.0	35.19021	1
TCAACGC	16760	0.0	29.491821	4
ATCAACG	16865	0.0	29.282944	3
CAACGCA	17140	0.0	28.81312	5
AACGCAG	17805	0.0	27.78484	6
TATCAAC	18020	0.0	27.645353	2
ACGCAGA	20550	0.0	24.031963	7
CGCAGAG	21235	0.0	23.25674	8
GCAGAGT	24690	0.0	19.985044	9
GAGTACT	13580	0.0	19.218697	12-13
GTGGTAT	4745	0.0	18.860165	1
GTACATG	19065	0.0	18.731373	1
TGGTATC	4455	0.0	18.653027	2
TACATGG	19365	0.0	18.023071	2
AGAGTAC	21845	0.0	17.945412	10-11
CAGAGTA	23935	0.0	17.749224	10-11
ACATGGG	18980	0.0	17.623457	3
GTACTTT	15265	0.0	17.125326	14-15
TATACCG	425	1.8189894E-12	17.04424	5
>>END_MODULE