FastQCFastQC Report
Fri 27 May 2016
SRR2139483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659358
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.043.4539071
GTATCAA93750.037.0175821
TCAACGC110100.030.9015644
ATCAACG112050.030.439833
CAACGCA113650.029.9363145
TATCAAC117350.029.1392252
AACGCAG119950.028.4347386
ACGCAGA138500.024.59577
CGCAGAG141800.024.053228
CGAGACC40800.023.807686-87
AGACCGA42850.023.52915688-89
GAGACCG41850.023.41655288-89
GACCGAG44450.022.52799490-91
ACCGAGG45650.022.43862790-91
ACGAGAC45300.021.31937486-87
GCCCACG45800.020.89980382-83
CACGAGA46300.020.85852284-85
TCTCCGA44200.020.83983474-75
CCCACGA46500.020.8197782-83
GCAGAGT164350.020.7271339
Produced by FastQC (version 0.11.5)