##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4659358
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.957390910936656	32.0	18.0	33.0	18.0	33.0
2	29.235836997285894	31.0	27.0	33.0	18.0	33.0
3	31.10635842963773	33.0	30.0	33.0	28.0	33.0
4	32.266541656597326	33.0	32.0	33.0	31.0	33.0
5	32.84940371613428	33.0	33.0	33.0	32.0	34.0
6	36.663749383498754	38.0	37.0	38.0	34.0	38.0
7	37.19725957953864	38.0	38.0	38.0	36.0	38.0
8	37.40684510612836	38.0	38.0	38.0	37.0	38.0
9	37.386981854581684	38.0	38.0	38.0	37.0	38.0
10-11	37.443888406943614	38.0	38.0	38.0	37.0	38.0
12-13	37.49249156214225	38.0	38.0	38.0	37.0	38.0
14-15	37.52085416059465	38.0	38.0	38.0	37.0	38.0
16-17	37.55121810773072	38.0	38.0	38.0	38.0	38.0
18-19	37.547941905301116	38.0	38.0	38.0	38.0	38.0
20-21	37.55522419870039	38.0	38.0	38.0	38.0	38.0
22-23	37.56115977872079	38.0	38.0	38.0	38.0	38.0
24-25	37.57183403105886	38.0	38.0	38.0	38.0	38.0
26-27	37.54463736627588	38.0	38.0	38.0	38.0	38.0
28-29	37.519637693014204	38.0	38.0	38.0	38.0	38.0
30-31	37.52341201079567	38.0	38.0	38.0	38.0	38.0
32-33	37.514566569790716	38.0	38.0	38.0	38.0	38.0
34-35	37.40947784380411	38.0	38.0	38.0	37.0	38.0
36-37	37.45634219992134	38.0	38.0	38.0	37.0	38.0
38-39	37.46132443272752	38.0	38.0	38.0	37.0	38.0
40-41	37.464688757700415	38.0	38.0	38.0	38.0	38.0
42-43	37.438391970201	38.0	38.0	38.0	37.0	38.0
44-45	37.41050481519045	38.0	38.0	38.0	37.0	38.0
46-47	37.38797569273417	38.0	38.0	38.0	37.0	38.0
48-49	37.33210344221372	38.0	38.0	38.0	37.0	38.0
50-51	37.3028209993932	38.0	38.0	38.0	37.0	38.0
52-53	37.2807931912718	38.0	38.0	38.0	37.0	38.0
54-55	37.25479538795905	38.0	38.0	38.0	37.0	38.0
56-57	37.20947047813169	38.0	38.0	38.0	37.0	38.0
58-59	37.205015053980645	38.0	38.0	38.0	37.0	38.0
60-61	37.20445764677963	38.0	38.0	38.0	37.0	38.0
62-63	37.210355108949216	38.0	38.0	38.0	37.0	38.0
64-65	37.21909084789098	38.0	38.0	38.0	37.0	38.0
66-67	37.21627391313995	38.0	38.0	38.0	37.0	38.0
68-69	37.20713596657497	38.0	38.0	38.0	36.0	38.0
70-71	37.203580106673876	38.0	38.0	38.0	36.0	38.0
72-73	37.1912558567557	38.0	38.0	38.0	36.0	38.0
74-75	37.19135818218791	38.0	38.0	38.0	36.0	38.0
76-77	37.1754963074703	38.0	38.0	38.0	36.0	38.0
78-79	37.16550134569353	38.0	38.0	38.0	36.0	38.0
80-81	37.150398920677645	38.0	38.0	38.0	36.0	38.0
82-83	37.10347979451359	38.0	38.0	38.0	36.0	38.0
84-85	37.09373466101644	38.0	38.0	38.0	36.0	38.0
86-87	37.086482402577985	38.0	38.0	38.0	36.0	38.0
88-89	37.067608529441436	38.0	38.0	38.0	36.0	38.0
90-91	37.05804678599291	38.0	38.0	38.0	36.0	38.0
92-93	37.03992963028344	38.0	38.0	38.0	36.0	38.0
94-95	37.0215199703823	38.0	38.0	38.0	36.0	38.0
96-97	37.00455682617969	38.0	38.0	38.0	36.0	38.0
98-99	37.01207432106246	38.0	38.0	38.0	36.0	38.0
100	35.64148294353583	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	12.0
21	56.0
22	180.0
23	565.0
24	1345.0
25	2961.0
26	5395.0
27	9122.0
28	14732.0
29	21860.0
30	29754.0
31	38614.0
32	50406.0
33	67896.0
34	103864.0
35	195724.0
36	659339.0
37	3457526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49731744158744	21.54303232333725	14.38206722900451	23.577583006070792
2	14.271792915758263	21.861549032061557	41.89115310124733	21.975504950932844
3	18.503045727332392	26.572216424993034	30.384451444998128	24.540286402676443
4	12.899244917432831	18.3640321263144	39.20089420044564	29.53582875580713
5	13.482909018796152	38.26748234413411	35.24859004180404	13.001018595265702
6	29.778694833064982	38.640967274890656	18.69098704156238	12.889350850481977
7	26.465362824663828	33.25249959329161	23.122284228857282	17.159853353187284
8	24.017386086237632	37.9127982868026	21.007422052566042	17.062393574393724
9	25.403800266045234	17.87937308101245	21.725525276229043	34.991301376713274
10-11	24.152522729526257	27.573530516435955	28.846055615387357	19.427891138650434
12-13	24.90125249014993	24.9471815644988	29.119505305237332	21.03206064011394
14-15	22.09699276166373	26.528933814486887	26.599351241093732	24.774722182755653
16-17	21.672567336530054	29.72894548991513	27.30262409542259	21.295863078132225
18-19	21.698064411448957	28.746481811442692	29.02571555995483	20.529738217153522
20-21	22.430497159877458	27.93432111992644	28.94406377258759	20.691117947608507
22-23	22.250349715639732	27.789308869604223	28.822503282524625	21.137838132231415
24-25	22.025890046826945	27.936761728630376	28.855678303858284	21.18166992068439
26-27	21.92119437408726	28.11011484052479	28.828412838559732	21.140277946828217
28-29	21.885221901939317	28.03484695190635	28.89025892236989	21.18967222378445
30-31	22.10689998470594	27.970532229997268	28.808577827785896	21.113989957510892
32-33	21.9415250988561	28.16853043893283	28.715162050114667	21.174782412096405
34-35	22.029281373868955	28.181699254759447	28.708553981409334	21.080465389962267
36-37	22.068030065933343	28.006153894229318	28.730188043372824	21.195627996464516
38-39	21.82876447416898	28.161255034742812	28.884185464168183	21.125795026920027
40-41	22.060799308369692	28.111388186854956	28.7321096780201	21.09570282675525
42-43	22.02577323696304	28.144138295677063	28.549277887056878	21.280810580303015
44-45	21.968427783264957	28.298554986595605	28.476390940379464	21.25662628975997
46-47	22.146682674410954	28.265498714242838	28.19644503692327	21.391373574422943
48-49	22.05623705769344	28.31910262278464	28.199670159483553	21.424990160038366
50-51	21.984538509383665	28.465654137032026	28.155761391187777	21.39404596239653
52-53	22.19097126084315	28.430117993605812	28.049218241061176	21.329692504489863
54-55	22.1731154676023	28.36481964599557	28.030479668859748	21.431585217542377
56-57	22.12747078251211	28.473858742681152	28.028022260428003	21.370648214378736
58-59	22.032378584453358	28.41779243470143	28.001474693061446	21.54835428778377
60-61	22.29742024987819	28.51760420872582	28.05618073729019	21.128794804105794
62-63	21.977221663595557	28.671620535864633	28.089087856204685	21.262069944335124
64-65	22.031309785281756	28.649231405120148	27.96823514121295	21.351223668385146
66-67	22.0869244511318	28.98688205613294	27.94101213771268	20.98518135502258
68-69	22.04867079223885	28.950947564894452	27.87003048810303	21.130351154763673
70-71	22.108409054676788	28.705419676896128	27.659965848124514	21.52620542030257
72-73	22.14553560790062	28.700805498263705	27.582971431260194	21.570687462575485
74-75	21.956746354564814	28.935143548591498	27.790170815261163	21.31793928158253
76-77	22.107103892288382	28.048390402984	28.017190858598035	21.82731484612958
78-79	22.039886367046964	28.07413806377695	27.736423224028385	22.149552345147697
80-81	22.29336922461088	28.438910237975485	27.66288630118925	21.604834236224384
82-83	22.344036702146973	28.04069822085798	27.784048066098897	21.83121701089615
84-85	22.116364150567122	28.0011728059071	27.77737898658883	22.105084056936946
86-87	22.050908033827792	28.29094769520082	27.844879963261963	21.813264307709424
88-89	22.348611492749903	28.28957971906268	27.78409770087923	21.577711087308188
90-91	22.433440661603452	28.26059843416052	27.75980602786815	21.546154876367876
92-93	22.190879610628762	27.97258905078336	27.769610344981917	22.066920993605965
94-95	22.316255742342946	28.266087490801535	27.82917646050197	21.58848030635355
96-97	22.725503574230455	28.065181090373382	27.92173842575851	21.287576909637657
98-99	23.250518825410165	30.177180887301002	28.282849458823243	18.289450828465583
100	29.37692823490279	40.97471669356443	29.648355071532784	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	924.0
1	798.0
2	723.5
3	845.0
4	1098.0
5	1574.5
6	1974.0
7	2433.5
8	3048.0
9	3508.0
10	3771.5
11	4126.0
12	4941.5
13	6249.0
14	8670.5
15	11707.0
16	14012.0
17	15270.0
18	15429.0
19	14775.5
20	13964.5
21	13720.5
22	14824.0
23	17880.5
24	23199.0
25	30804.5
26	40530.5
27	51492.0
28	62705.5
29	74823.0
30	86614.5
31	97353.0
32	110599.5
33	124973.5
34	137196.0
35	148905.0
36	161381.5
37	171651.5
38	179560.0
39	185989.5
40	191777.0
41	196229.0
42	197722.5
43	199622.0
44	202342.0
45	203290.5
46	202971.5
47	200308.0
48	195196.0
49	189577.0
50	183657.5
51	176922.0
52	167156.5
53	155940.5
54	143676.0
55	131245.5
56	118026.5
57	104436.5
58	91726.5
59	77855.0
60	64240.5
61	51541.5
62	40314.0
63	30686.5
64	22597.5
65	16669.5
66	12390.5
67	9441.5
68	7125.5
69	5214.5
70	3795.5
71	2694.5
72	1936.0
73	1337.5
74	884.5
75	581.0
76	370.5
77	229.0
78	153.0
79	107.0
80	74.0
81	46.5
82	30.5
83	23.5
84	12.5
85	5.5
86	3.5
87	3.5
88	5.0
89	6.5
90	6.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011568117324318072
3	9.443361081075976E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.511842688278167E-5
22-23	0.0
24-25	1.073223691370058E-4
26-27	2.039334468562854E-4
28-29	0.0017286173788105008
30-31	0.0023751978615051145
32-33	0.001971105107079477
34-35	4.53708107753083E-4
36-37	0.0
38-39	0.0
40-41	1.978846787457673E-4
42-43	0.001870895998662697
44-45	2.567457432392985E-4
46-47	4.957328666834615E-4
48-49	9.095202439879007E-4
50-51	0.0010322387664609108
52-53	0.0
54-55	0.002889691503293258
56-57	0.05151245407305671
58-59	0.0686423757078893
60-61	0.044625882773622574
62-63	0.007516839223227875
64-65	0.0
66-67	0.0
68-69	1.1141037864426444E-4
70-71	0.0014265199570141986
72-73	0.0072219197279195495
74-75	0.011802581137635978
76-77	0.013074475075313752
78-79	0.0038624316669289465
80-81	0.0077069856830863735
82-83	0.04531777689882286
84-85	0.03892545249826953
86-87	0.009120956563387171
88-89	0.002371351100727412
90-91	0.0029435353198430133
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	133.0
22-23	280.0
24-25	377.0
26-27	1056.0
28-29	2983.0
30-31	9654.0
32-33	12430.0
34-35	17748.0
36-37	28029.0
38-39	30990.0
40-41	30889.0
42-43	35543.0
44-45	41079.0
46-47	40328.0
48-49	39027.0
50-51	35694.0
52-53	33207.0
54-55	36413.0
56-57	38134.0
58-59	20752.0
60-61	38122.0
62-63	36569.0
64-65	39162.0
66-67	41026.0
68-69	42835.0
70-71	43734.0
72-73	38968.0
74-75	39920.0
76-77	41655.0
78-79	43208.0
80-81	42388.0
82-83	40028.0
84-85	39632.0
86-87	40276.0
88-89	42125.0
90-91	42900.0
92-93	41853.0
94-95	46966.0
96-97	157965.0
98-99	1032452.0
100-101	2272804.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.85203877913173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.5982817739972	83.20144404838163
2	5.568101142211373	10.006104795122079
3	1.115859988459717	3.0078688486549185
4	0.3616010619276836	1.2996237055560576
5	0.1401802192574167	0.6297739248392297
6	0.07490913432513349	0.40384430653758496
7	0.044110636099883395	0.2774401409793223
8	0.02462141235157694	0.17698272779287072
9	0.01585537843274921	0.12821742700174715
>10	0.05533928656093396	0.7612569565609881
>50	8.460938528045782E-4	0.05537514911150492
>100	2.938725235431634E-4	0.052067969462052235
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.1462184275172675E-5	0.0
4	0.0	4.292436855034535E-5	0.0	2.1462184275172675E-5	0.0
5	0.0	8.58487371006907E-5	0.0	2.1462184275172675E-5	0.0
6	0.0	8.58487371006907E-5	0.0	2.1462184275172675E-5	0.0
7	0.0	8.58487371006907E-5	0.0	2.1462184275172675E-5	0.0
8	0.0	8.58487371006907E-5	0.0	2.1462184275172675E-5	2.1462184275172675E-5
9	0.0	1.0731092137586337E-4	0.0	8.58487371006907E-5	2.1462184275172675E-5
10-11	0.0	1.0731092137586337E-4	0.0	1.0731092137586337E-4	2.1462184275172675E-5
12-13	0.0	1.0731092137586337E-4	0.0	1.2877310565103604E-4	2.1462184275172675E-5
14-15	1.0731092137586338E-5	1.5023528992620872E-4	0.0	1.2877310565103604E-4	2.1462184275172675E-5
16-17	3.219327641275901E-5	1.6096638206379506E-4	0.0	1.5023528992620872E-4	2.1462184275172675E-5
18-19	4.292436855034535E-5	1.8242856633896774E-4	0.0	1.716974742013814E-4	3.219327641275901E-5
20-21	4.292436855034535E-5	1.9315965847655406E-4	0.0	2.038907506141404E-4	4.292436855034535E-5
22-23	4.292436855034535E-5	2.1462184275172674E-4	0.0	2.1462184275172674E-4	5.3655460687931685E-5
24-25	5.3655460687931685E-5	2.4681511916448573E-4	0.0	2.575462113020721E-4	6.438655282551802E-5
26-27	8.58487371006907E-5	2.575462113020721E-4	0.0	3.433949484027628E-4	6.438655282551802E-5
28-29	8.58487371006907E-5	2.575462113020721E-4	0.0	6.224033439800076E-4	6.438655282551802E-5
30-31	1.0731092137586337E-4	3.6485713267793543E-4	0.0	0.001781361294839332	6.438655282551802E-5
32-33	1.0731092137586337E-4	3.863193169531081E-4	0.0	0.004421209960685571	6.438655282551802E-5
34-35	1.0731092137586337E-4	3.9705040909069445E-4	0.0	0.007833697260438026	8.58487371006907E-5
36-37	1.0731092137586337E-4	4.1851259336586714E-4	0.0	0.013435327356258093	8.58487371006907E-5
38-39	1.2877310565103604E-4	4.292436855034535E-4	0.0	0.028598360546667586	8.58487371006907E-5
40-41	1.2877310565103604E-4	4.7216805405379884E-4	0.0	0.044072595409067085	8.58487371006907E-5
42-43	1.2877310565103604E-4	4.8289914619138513E-4	0.0	0.052614544750585815	8.58487371006907E-5
44-45	1.2877310565103604E-4	5.258235147417305E-4	0.0	0.06390365367932663	8.58487371006907E-5
46-47	1.2877310565103604E-4	5.580167911544895E-4	0.0	0.07554688864860781	8.58487371006907E-5
48-49	1.2877310565103604E-4	5.580167911544895E-4	0.0	0.08692184631444932	1.0731092137586337E-4
50-51	1.2877310565103604E-4	6.653277125303529E-4	0.0	0.09804998886112636	1.0731092137586337E-4
52-53	1.2877310565103604E-4	6.653277125303529E-4	0.0	0.1317134248967347	1.180420135134497E-4
54-55	1.2877310565103604E-4	6.760588046679393E-4	0.0	0.17257742375666346	1.5023528992620872E-4
56-57	1.2877310565103604E-4	7.082520810806983E-4	0.0	0.22601826260184343	1.8242856633896774E-4
58-59	1.2877310565103604E-4	7.082520810806983E-4	0.0	0.46231691147149456	1.9315965847655406E-4
60-61	1.2877310565103604E-4	7.082520810806983E-4	0.0	0.6774109222772751	1.9315965847655406E-4
62-63	1.2877310565103604E-4	7.082520810806983E-4	0.0	0.6853197371826762	1.9315965847655406E-4
64-65	1.2877310565103604E-4	7.511764496310435E-4	0.0	0.6925739554676846	1.9315965847655406E-4
66-67	1.2877310565103604E-4	7.726386339062162E-4	0.0	0.6991199216716123	2.038907506141404E-4
68-69	1.2877310565103604E-4	8.048319103189752E-4	0.0	0.7053761483878251	2.1462184275172674E-4
70-71	1.2877310565103604E-4	8.370251867317343E-4	0.0	0.7105914591666921	2.575462113020721E-4
72-73	1.2877310565103604E-4	8.58487371006907E-4	0.0	0.7146800052711124	2.7900839557724476E-4
74-75	1.2877310565103604E-4	9.121428316948387E-4	0.0	0.7179959127416267	3.0047057985241744E-4
76-77	1.2877310565103604E-4	9.443361081075977E-4	0.0	0.7214727865942046	3.0047057985241744E-4
78-79	1.5023528992620872E-4	9.443361081075977E-4	0.0	0.7237263159430978	3.0047057985241744E-4
80-81	1.5023528992620872E-4	9.443361081075977E-4	0.0	0.7245740722219671	3.112016719900038E-4
82-83	1.5023528992620872E-4	9.55067200245184E-4	0.0	0.725014046999608	3.6485713267793543E-4
84-85	1.5023528992620872E-4	9.979915687955293E-4	0.0	0.7252715932109102	3.6485713267793543E-4
86-87	1.5023528992620872E-4	0.0010301848452082883	0.0	0.725432559592974	3.6485713267793543E-4
88	1.5023528992620872E-4	0.0010945713980338063	0.0	0.7254432906851116	3.6485713267793543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4950	0.0	43.453907	1
GTATCAA	9375	0.0	37.017582	1
TCAACGC	11010	0.0	30.901564	4
ATCAACG	11205	0.0	30.43983	3
CAACGCA	11365	0.0	29.936314	5
TATCAAC	11735	0.0	29.139225	2
AACGCAG	11995	0.0	28.434738	6
ACGCAGA	13850	0.0	24.5957	7
CGCAGAG	14180	0.0	24.05322	8
CGAGACC	4080	0.0	23.8076	86-87
AGACCGA	4285	0.0	23.529156	88-89
GAGACCG	4185	0.0	23.416552	88-89
GACCGAG	4445	0.0	22.527994	90-91
ACCGAGG	4565	0.0	22.438627	90-91
ACGAGAC	4530	0.0	21.319374	86-87
GCCCACG	4580	0.0	20.899803	82-83
CACGAGA	4630	0.0	20.858522	84-85
TCTCCGA	4420	0.0	20.839834	74-75
CCCACGA	4650	0.0	20.81977	82-83
GCAGAGT	16435	0.0	20.727133	9
>>END_MODULE