FastQCFastQC Report
Fri 27 May 2016
SRR2139482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181004
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT43800.065.0480494
TATCTCG54450.048.80480294
GGTATCA49350.047.5936621
GCCGTCT25100.037.2133890-91
CCGTCTT24850.036.90846690-91
TGCCGTC24350.036.8551188-89
TATGCCG25300.035.07031286-87
TCTCGTA24500.034.98788582-83
ATGCCGT26950.034.12172788-89
GTATCAA84350.033.7395061
CTCGTAT26850.032.22493482-83
TCGTATG29300.030.87181384-85
CGTATGC28850.030.69624184-85
TCAACGC95650.029.2567984
ATCAACG97850.028.5990053
CAACGCA98750.028.3383585
CTATCTC69500.028.25615992-93
AACGCAG101300.027.6250026
TATCAAC104250.026.9290452
GTATGCC34650.026.2391686-87
Produced by FastQC (version 0.11.5)