##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4181004
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.49367759514222	32.0	18.0	33.0	18.0	33.0
2	28.972084217092355	31.0	27.0	33.0	18.0	33.0
3	31.019323349128584	32.0	30.0	33.0	27.0	33.0
4	32.26015425959889	33.0	32.0	33.0	31.0	33.0
5	32.86295444826171	33.0	33.0	33.0	32.0	34.0
6	36.70645423922101	38.0	37.0	38.0	34.0	38.0
7	37.22715668294027	38.0	38.0	38.0	36.0	38.0
8	37.41124284980354	38.0	38.0	38.0	37.0	38.0
9	37.365610748040424	38.0	38.0	38.0	37.0	38.0
10-11	37.4309913360523	38.0	38.0	38.0	37.0	38.0
12-13	37.47972556352494	38.0	38.0	38.0	37.0	38.0
14-15	37.511377291196084	38.0	38.0	38.0	37.0	38.0
16-17	37.545540377383034	38.0	38.0	38.0	37.5	38.0
18-19	37.54121223036381	38.0	38.0	38.0	38.0	38.0
20-21	37.55043107509031	38.0	38.0	38.0	38.0	38.0
22-23	37.55540226669812	38.0	38.0	38.0	38.0	38.0
24-25	37.567264910603875	38.0	38.0	38.0	38.0	38.0
26-27	37.53896487955059	38.0	38.0	38.0	38.0	38.0
28-29	37.51307707054457	38.0	38.0	38.0	37.5	38.0
30-31	37.51851964551031	38.0	38.0	38.0	38.0	38.0
32-33	37.50701262110192	38.0	38.0	38.0	38.0	38.0
34-35	37.40858016825503	38.0	38.0	38.0	37.0	38.0
36-37	37.448939259945476	38.0	38.0	38.0	37.0	38.0
38-39	37.45654316751184	38.0	38.0	38.0	37.0	38.0
40-41	37.45337159646219	38.0	38.0	38.0	37.0	38.0
42-43	37.42680773210624	38.0	38.0	38.0	37.0	38.0
44-45	37.400715144047794	38.0	38.0	38.0	37.0	38.0
46-47	37.37754130085592	38.0	38.0	38.0	37.0	38.0
48-49	37.32095316533076	38.0	38.0	38.0	37.0	38.0
50-51	37.28599299949491	38.0	38.0	38.0	37.0	38.0
52-53	37.265233831255756	38.0	38.0	38.0	37.0	38.0
54-55	37.23639754340542	38.0	38.0	38.0	37.0	38.0
56-57	37.19013169755091	38.0	38.0	38.0	37.0	38.0
58-59	37.18410787597881	38.0	38.0	38.0	37.0	38.0
60-61	37.181989113048374	38.0	38.0	38.0	36.5	38.0
62-63	37.185315118621816	38.0	38.0	38.0	36.0	38.0
64-65	37.19155106687734	38.0	38.0	38.0	36.0	38.0
66-67	37.186362334357554	38.0	38.0	38.0	36.0	38.0
68-69	37.17403874927105	38.0	38.0	38.0	36.0	38.0
70-71	37.16913192897485	38.0	38.0	38.0	36.0	38.0
72-73	37.15870112279781	38.0	38.0	38.0	36.0	38.0
74-75	37.15002191118904	38.0	38.0	38.0	36.0	38.0
76-77	37.13041850591267	38.0	38.0	38.0	36.0	38.0
78-79	37.11229129398083	38.0	38.0	38.0	36.0	38.0
80-81	37.097132558084525	38.0	38.0	38.0	36.0	38.0
82-83	37.01922407981677	38.0	38.0	38.0	36.0	38.0
84-85	37.03722305207579	38.0	38.0	38.0	36.0	38.0
86-87	37.02917704202398	38.0	38.0	38.0	36.0	38.0
88-89	37.008304766236634	38.0	38.0	38.0	36.0	38.0
90-91	37.00750369809049	38.0	38.0	38.0	36.0	38.0
92-93	36.98699508300439	38.0	38.0	38.0	36.0	38.0
94-95	36.93135391495089	38.0	38.0	38.0	35.0	38.0
96-97	36.85801671140737	38.0	38.0	38.0	35.0	38.0
98-99	36.89345541391674	38.0	38.0	38.0	35.0	38.0
100	35.520675951261204	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	19.0
21	44.0
22	186.0
23	493.0
24	1324.0
25	2707.0
26	5115.0
27	8438.0
28	13785.0
29	20205.0
30	27147.0
31	35495.0
32	45975.0
33	63012.0
34	95936.0
35	182787.0
36	616961.0
37	3061372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88467746024639	21.181132570071686	13.9162746555612	24.01791531412072
2	14.32764317022553	21.616878935488838	41.83169287854128	22.223785015744348
3	18.726946227516667	26.14043672951787	30.16905581602043	24.963561226945032
4	12.86726824466085	17.950353551443623	39.02251707962968	30.159861124265845
5	13.542656261510395	37.98613921440879	35.3957087819098	13.07549574217102
6	30.25084405563831	38.32335486883055	18.568745688834547	12.857055386696592
7	26.788063345550494	32.84213552534272	23.11896855396455	17.25083257514224
8	24.073093448367906	37.85526155918531	20.974483640771453	17.09716135167534
9	25.55821520381229	17.421365777215232	21.523562283126253	35.49685673584622
10-11	24.322052789234355	27.170543247507055	28.938910366983624	19.568493596274962
12-13	25.018141575564144	24.56504466391326	29.231818481876605	21.184995278645992
14-15	22.133583225464506	26.15384964951002	26.600656206021327	25.111910919004142
16-17	21.78267468770659	29.28507124126167	27.358787506541493	21.57346656449025
18-19	21.842169966830934	28.44270180081148	28.974177015855524	20.740951216502065
20-21	22.47893579655905	27.63640233988903	28.93416416176399	20.950497701787924
22-23	22.305588287669096	27.52672349676481	28.75707889675828	21.41060931880781
24-25	22.132625103873494	27.620374848764495	28.808072878170083	21.438927169191924
26-27	22.071637528502517	27.754330115677483	28.74817872768287	21.42585362813713
28-29	22.024551630423403	27.748112032055516	28.81299164616589	21.41434469135519
30-31	22.199208301975222	27.684512098900676	28.74084025607868	21.375439343045425
32-33	21.97057825282074	27.90061392988387	28.677268020423814	21.451539796871575
34-35	22.149089989876593	27.913973457445667	28.650215709762595	21.286720842915148
36-37	22.119032069262033	27.715406820962745	28.661504898908923	21.504056210866302
38-39	21.93052054207562	27.837881264843112	28.71851677827023	21.513081414811037
40-41	22.303653486261197	27.6931878589144	28.7151963373316	21.287962317492802
42-43	22.007617568883198	27.748916996029884	28.56506923195619	21.678396203130728
44-45	22.01312734942438	27.852136339186362	28.494754572368215	21.639981739021046
46-47	22.112084104862156	27.84968241391812	28.45013342448212	21.588100056737606
48-49	22.04015239855906	28.085296087233115	28.276828533347242	21.59772298086059
50-51	21.998678144945504	28.259045164661895	27.996685115425358	21.74559157496724
52-53	22.031679974643076	28.03153896013746	27.953197568129077	21.983583497090386
54-55	22.243448150814835	27.73547084402536	28.31615262467831	21.704928380481494
56-57	21.990823698362398	27.406894678089795	28.51212602930458	22.090155594243228
58-59	21.844932623332962	27.176255404485257	28.453496648865173	22.525315323316615
60-61	22.077095627906687	27.52267663351356	28.825173305844302	21.57505443273545
62-63	21.573124285115306	27.81228085915114	28.831788497971182	21.782806357762368
64-65	21.373081315224727	28.218947273978323	28.389482371552766	22.018489039244184
66-67	21.474125063524717	29.074331645378116	27.758317876095635	21.69322541500153
68-69	21.663835979597533	28.966080450341554	27.61187042995146	21.758213140109458
70-71	21.669429993500135	28.390294837322553	27.598879663300924	22.341395505876385
72-73	21.83980805021408	27.6922223482432	27.615012375839964	22.85295722570275
74-75	21.87060834046027	27.839903444315716	27.846239009977058	22.443249205246957
76-77	22.27793753441566	27.503727356719203	27.23379238612573	22.984542722739405
78-79	22.449853589756756	27.50827549997857	26.719398971697416	23.32247193856726
80-81	22.224880995272994	28.018706637704	26.936339676199584	22.820072690823423
82-83	22.16500929505149	27.698660390120228	27.00344087589298	23.1328894389353
84-85	22.191504327438828	28.142028596718703	27.0038641134237	22.66260296241877
86-87	22.8744800379273	27.957555188539416	27.057372118213824	22.110592655319465
88-89	22.51380548139347	28.194929579817096	27.452747019399848	21.838517919389588
90-91	22.49730880446975	27.68699548566842	27.21095021748238	22.604745492379443
92-93	22.476702745149723	27.164159843604992	27.53514952160773	22.823987889637547
94-95	21.639493358833175	27.696112205066132	28.24611071599798	22.418283720102714
96-97	21.80526197309569	27.533903156770002	28.463100786762674	22.197734083371635
98-99	22.98729216944981	29.33987551213228	28.84688975851553	18.82594255990238
100	29.54158080723513	40.53641133547752	29.922007857287348	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	973.0
1	822.5
2	719.0
3	859.0
4	1110.5
5	1484.5
6	1821.5
7	2338.0
8	3017.5
9	3399.5
10	3507.0
11	3703.5
12	4204.0
13	5277.0
14	7449.0
15	9994.0
16	11840.5
17	12761.5
18	12919.0
19	12679.0
20	12303.0
21	12153.0
22	12900.5
23	15433.5
24	19882.0
25	25607.0
26	32989.0
27	41722.5
28	50708.0
29	60705.5
30	70357.0
31	79381.0
32	90062.0
33	101517.0
34	111923.5
35	122328.5
36	133349.0
37	142529.5
38	150537.0
39	157270.0
40	163620.0
41	169769.0
42	173036.5
43	176011.5
44	178954.5
45	181314.0
46	182060.5
47	180887.0
48	179005.0
49	176117.5
50	172247.0
51	166043.0
52	157144.0
53	146761.0
54	135775.5
55	124201.5
56	111591.0
57	98563.0
58	85997.0
59	72648.5
60	59398.0
61	47477.5
62	37447.0
63	28818.5
64	21161.0
65	15553.5
66	11546.5
67	8714.5
68	6558.5
69	4874.0
70	3588.0
71	2588.5
72	1849.0
73	1241.0
74	788.5
75	510.0
76	332.0
77	214.0
78	143.5
79	89.5
80	59.0
81	44.0
82	35.5
83	29.0
84	20.0
85	10.0
86	4.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010906471268623517
3	4.30518602708823E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.076304744446985E-4
22-23	0.0
24-25	2.2724144080163124E-4
26-27	3.708019537196101E-4
28-29	0.0018905946386804416
30-31	0.0023360799643765773
32-33	0.0025340569447031545
34-35	6.024366393288952E-4
36-37	0.0
38-39	4.869086099319131E-5
40-41	1.8364238510322354E-4
42-43	0.0021214968936721793
44-45	2.4885436781098616E-4
46-47	3.895590141693923E-4
48-49	0.0011040309311396873
50-51	9.734496725238449E-4
52-53	0.0
54-55	0.0028912459753856023
56-57	0.053766205715519946
58-59	0.0692529861491455
60-61	0.04559691729076826
62-63	0.007663212031399815
64-65	0.0
66-67	0.0
68-69	1.0761352217712916E-4
70-71	0.0011961133926369977
72-73	0.007318140434428432
74-75	0.011768626311598848
76-77	0.01265510022237419
78-79	0.004017861374445298
80-81	0.007808840723247392
82-83	0.04570219374867437
84-85	0.038763448901186215
86-87	0.008822954435637064
88-89	0.002326209763281316
90-91	0.0027295774448229493
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	15.0
20-21	110.0
22-23	252.0
24-25	329.0
26-27	1068.0
28-29	3180.0
30-31	10013.0
32-33	12334.0
34-35	17712.0
36-37	27990.0
38-39	16339.0
40-41	30192.0
42-43	33526.0
44-45	39016.0
46-47	39377.0
48-49	37233.0
50-51	18537.0
52-53	2205.0
54-55	2038.0
56-57	1883.0
58-59	19599.0
60-61	36070.0
62-63	33948.0
64-65	34650.0
66-67	36910.0
68-69	38351.0
70-71	37633.0
72-73	34944.0
74-75	34810.0
76-77	37051.0
78-79	37931.0
80-81	38493.0
82-83	35428.0
84-85	34198.0
86-87	35303.0
88-89	37251.0
90-91	38010.0
92-93	37120.0
94-95	41839.0
96-97	142246.0
98-99	999508.0
100-101	2066362.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.73755490410161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.29731458240371	82.82535334839591
2	5.834261352292891	10.471046968525227
3	1.1454726176311758	3.083757357474679
4	0.3765646462852441	1.351679624838628
5	0.15501017417617896	0.6955117007914606
6	0.06530243108142912	0.35160482967246337
7	0.04189696918931642	0.2631812101059215
8	0.019000816388044444	0.13640694430759134
9	0.01882368864732993	0.15202726141387354
>10	0.045628989656028435	0.6137704892417092
>50	5.079454330957331E-4	0.03369083428631586
>100	2.157868155550188E-4	0.021969430946217366
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3917700150490168E-5
2	0.0	2.3917700150490168E-5	0.0	0.0	4.7835400300980337E-5
3	0.0	2.3917700150490168E-5	0.0	0.0	4.7835400300980337E-5
4	0.0	9.567080060196067E-5	0.0	2.3917700150490168E-5	4.7835400300980337E-5
5	0.0	9.567080060196067E-5	0.0	2.3917700150490168E-5	4.7835400300980337E-5
6	4.7835400300980337E-5	9.567080060196067E-5	0.0	2.3917700150490168E-5	4.7835400300980337E-5
7	4.7835400300980337E-5	9.567080060196067E-5	0.0	2.3917700150490168E-5	4.7835400300980337E-5
8	4.7835400300980337E-5	9.567080060196067E-5	0.0	2.3917700150490168E-5	4.7835400300980337E-5
9	4.7835400300980337E-5	9.567080060196067E-5	0.0	2.3917700150490168E-5	4.7835400300980337E-5
10-11	4.7835400300980337E-5	9.567080060196067E-5	0.0	5.9794250376225426E-5	4.7835400300980337E-5
12-13	4.7835400300980337E-5	9.567080060196067E-5	0.0	8.371195052671559E-5	4.7835400300980337E-5
14-15	4.7835400300980337E-5	1.6742390105343118E-4	0.0	9.567080060196067E-5	4.7835400300980337E-5
16-17	8.371195052671559E-5	1.6742390105343118E-4	0.0	1.1958850075245085E-4	4.7835400300980337E-5
18-19	9.567080060196067E-5	1.6742390105343118E-4	0.0	1.1958850075245085E-4	4.7835400300980337E-5
20-21	9.567080060196067E-5	1.6742390105343118E-4	0.0	1.1958850075245085E-4	4.7835400300980337E-5
22-23	9.567080060196067E-5	2.152593013544115E-4	0.0	1.4350620090294102E-4	4.7835400300980337E-5
24-25	9.567080060196067E-5	2.272181514296566E-4	0.0	1.554650509781861E-4	4.7835400300980337E-5
26-27	9.567080060196067E-5	2.391770015049017E-4	0.0	3.3484780210686236E-4	4.7835400300980337E-5
28-29	9.567080060196067E-5	2.391770015049017E-4	0.0	9.088726057186265E-4	4.7835400300980337E-5
30-31	9.567080060196067E-5	3.109301019563722E-4	0.0	0.002511358515801468	4.7835400300980337E-5
32-33	9.567080060196067E-5	3.109301019563722E-4	0.0	0.005931589637321562	4.7835400300980337E-5
34-35	9.567080060196067E-5	3.3484780210686236E-4	0.0	0.010332446465011753	4.7835400300980337E-5
36-37	9.567080060196067E-5	3.707243523325976E-4	0.0	0.020617057529722527	4.7835400300980337E-5
38-39	9.567080060196067E-5	4.066009025583329E-4	0.0	0.21834468467382476	4.7835400300980337E-5
40-41	9.567080060196067E-5	5.022717031602935E-4	0.0	0.42147771205193774	4.7835400300980337E-5
42-43	1.1958850075245085E-4	5.381482533860288E-4	0.0	0.4440440621439252	5.9794250376225426E-5
44-45	1.1958850075245085E-4	5.859836536870092E-4	0.0	0.4742640762840695	7.175310045147051E-5
46-47	1.1958850075245085E-4	6.218602039127444E-4	0.0	0.5066964776881342	7.175310045147051E-5
48-49	1.1958850075245085E-4	6.218602039127444E-4	0.0	0.5396311507953593	7.175310045147051E-5
50-51	1.3154735082769592E-4	6.457779040632346E-4	0.0	0.5722429349505526	7.175310045147051E-5
52-53	1.4350620090294102E-4	6.457779040632346E-4	0.0	1.1496760108337614	7.175310045147051E-5
54-55	1.4350620090294102E-4	6.457779040632346E-4	0.0	1.9148630328983183	7.175310045147051E-5
56-57	1.4350620090294102E-4	6.457779040632346E-4	0.0	2.7532142997232247	7.175310045147051E-5
58-59	1.4350620090294102E-4	6.457779040632346E-4	0.0	3.614335217091397	7.175310045147051E-5
60-61	1.4350620090294102E-4	6.696956042137247E-4	0.0	3.839376857807359	7.175310045147051E-5
62-63	1.4350620090294102E-4	6.696956042137247E-4	0.0	3.8472338223067952	7.175310045147051E-5
64-65	1.4350620090294102E-4	7.653664048156854E-4	0.0	3.8544210912020174	7.175310045147051E-5
66-67	1.6742390105343118E-4	8.132018051166658E-4	0.0	3.8609147467928757	7.175310045147051E-5
68-69	1.6742390105343118E-4	8.371195052671559E-4	0.0	3.8673486081333577	7.175310045147051E-5
70-71	1.7938275112867625E-4	8.371195052671559E-4	0.0	3.872801843767669	7.175310045147051E-5
72-73	2.152593013544115E-4	8.49078355342401E-4	0.0	3.8770233178442304	1.4350620090294102E-4
74-75	2.152593013544115E-4	8.61037205417646E-4	0.0	3.8801326188637946	1.4350620090294102E-4
76-77	2.152593013544115E-4	8.849549055681362E-4	0.0	3.883182125632982	1.4350620090294102E-4
78-79	2.152593013544115E-4	9.088726057186264E-4	0.0	3.8851314181952468	1.4350620090294102E-4
80-81	2.152593013544115E-4	9.088726057186264E-4	0.0	3.8858011137994604	1.4350620090294102E-4
82-83	2.152593013544115E-4	9.088726057186264E-4	0.0	3.886494727103825	1.4350620090294102E-4
84-85	2.152593013544115E-4	9.088726057186264E-4	0.0	3.8869372045566086	1.4350620090294102E-4
86-87	2.152593013544115E-4	9.088726057186264E-4	0.0	3.8870209165071357	1.4350620090294102E-4
88	2.391770015049017E-4	9.567080060196068E-4	0.0	3.8870328753572108	1.4350620090294102E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	4380	0.0	65.04804	94
TATCTCG	5445	0.0	48.804802	94
GGTATCA	4935	0.0	47.593662	1
GCCGTCT	2510	0.0	37.21338	90-91
CCGTCTT	2485	0.0	36.908466	90-91
TGCCGTC	2435	0.0	36.85511	88-89
TATGCCG	2530	0.0	35.070312	86-87
TCTCGTA	2450	0.0	34.987885	82-83
ATGCCGT	2695	0.0	34.121727	88-89
GTATCAA	8435	0.0	33.739506	1
CTCGTAT	2685	0.0	32.224934	82-83
TCGTATG	2930	0.0	30.871813	84-85
CGTATGC	2885	0.0	30.696241	84-85
TCAACGC	9565	0.0	29.256798	4
ATCAACG	9785	0.0	28.599005	3
CAACGCA	9875	0.0	28.338358	5
CTATCTC	6950	0.0	28.256159	92-93
AACGCAG	10130	0.0	27.625002	6
TATCAAC	10425	0.0	26.929045	2
GTATGCC	3465	0.0	26.23916	86-87
>>END_MODULE