FastQCFastQC Report
Fri 27 May 2016
SRR2139481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4790385
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62900.049.266631
GTATCAA115200.035.804721
TCAACGC133900.030.6412354
ATCAACG135450.030.2590473
CAACGCA137250.029.9244845
AACGCAG141650.028.9949556
TATCAAC142050.028.8865382
TGGTATC30400.024.8865762
ACGCAGA164500.024.8634667
CGCAGAG169150.024.1799588
GTGGTAT33150.023.7246481
GCAGAGT195100.020.9638149
GAGTACT104500.020.16258612-13
CAGAGTA192550.018.50025610-11
GTACTTT116550.017.9680814-15
AGAGTAC177250.017.55338110-11
GTACATG145100.017.3505551
TACATGG149950.016.5043162
ACATGGG150250.015.9859133
AGTACTT117100.015.71201612-13
Produced by FastQC (version 0.11.5)