##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4790385
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.576088978234527	30.0	18.0	33.0	18.0	33.0
2	31.3267332375164	32.0	32.0	33.0	27.0	34.0
3	31.588936588604046	33.0	31.0	33.0	29.0	34.0
4	32.73693408776121	33.0	33.0	33.0	32.0	34.0
5	32.69559837048588	33.0	33.0	33.0	32.0	34.0
6	36.99423219636835	38.0	37.0	38.0	35.0	38.0
7	37.015874715706566	38.0	38.0	38.0	35.0	38.0
8	37.35685962610521	38.0	38.0	38.0	37.0	38.0
9	37.441496247170114	38.0	38.0	38.0	37.0	38.0
10-11	37.4465419794025	38.0	38.0	38.0	37.0	38.0
12-13	37.48435741594882	38.0	38.0	38.0	37.0	38.0
14-15	37.490130438367686	38.0	38.0	38.0	37.0	38.0
16-17	37.524778906079575	38.0	38.0	38.0	37.5	38.0
18-19	37.52444584307942	38.0	38.0	38.0	38.0	38.0
20-21	37.53133602375967	38.0	38.0	38.0	38.0	38.0
22-23	37.54214222451148	38.0	38.0	38.0	38.0	38.0
24-25	37.55415309373606	38.0	38.0	38.0	38.0	38.0
26-27	37.522626402620816	38.0	38.0	38.0	38.0	38.0
28-29	37.49778094353266	38.0	38.0	38.0	37.0	38.0
30-31	37.50309578093461	38.0	38.0	38.0	38.0	38.0
32-33	37.49204498513241	38.0	38.0	38.0	38.0	38.0
34-35	37.39343799053209	38.0	38.0	38.0	37.0	38.0
36-37	37.43669182874697	38.0	38.0	38.0	37.0	38.0
38-39	37.44525954639015	38.0	38.0	38.0	37.0	38.0
40-41	37.44078700625792	38.0	38.0	38.0	37.0	38.0
42-43	37.404984383522674	38.0	38.0	38.0	37.0	38.0
44-45	37.37384657681695	38.0	38.0	38.0	37.0	38.0
46-47	37.34521462650547	38.0	38.0	38.0	37.0	38.0
48-49	37.28370654983615	38.0	38.0	38.0	37.0	38.0
50-51	37.25253377227324	38.0	38.0	38.0	37.0	38.0
52-53	37.231619596202265	38.0	38.0	38.0	37.0	38.0
54-55	37.20283745445941	38.0	38.0	38.0	37.0	38.0
56-57	37.158233377530834	38.0	38.0	38.0	37.0	38.0
58-59	37.15306574198325	38.0	38.0	38.0	37.0	38.0
60-61	37.15517370128105	38.0	38.0	38.0	36.5	38.0
62-63	37.161659128161965	38.0	38.0	38.0	36.0	38.0
64-65	37.16760488755622	38.0	38.0	38.0	36.0	38.0
66-67	37.166081125273436	38.0	38.0	38.0	36.0	38.0
68-69	37.159504465887764	38.0	38.0	38.0	36.0	38.0
70-71	37.15475874891622	38.0	38.0	38.0	36.0	38.0
72-73	37.1447274092522	38.0	38.0	38.0	36.0	38.0
74-75	37.140299712843394	38.0	38.0	38.0	36.0	38.0
76-77	37.126041144264995	38.0	38.0	38.0	36.0	38.0
78-79	37.11209267821636	38.0	38.0	38.0	36.0	38.0
80-81	37.10272313255422	38.0	38.0	38.0	36.0	38.0
82-83	37.05550444039256	38.0	38.0	38.0	36.0	38.0
84-85	37.04533388050055	38.0	38.0	38.0	36.0	38.0
86-87	37.034757109616635	38.0	38.0	38.0	36.0	38.0
88-89	37.01784024139968	38.0	38.0	38.0	36.0	38.0
90-91	37.01039343550188	38.0	38.0	38.0	36.0	38.0
92-93	36.990953239794635	38.0	38.0	38.0	36.0	38.0
94-95	36.974347925715975	38.0	38.0	38.0	36.0	38.0
96-97	36.95892960541773	38.0	38.0	38.0	35.0	38.0
98-99	36.97195103447105	38.0	38.0	38.0	35.0	38.0
100	35.565559621016945	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	9.0
21	50.0
22	184.0
23	691.0
24	1547.0
25	3218.0
26	5828.0
27	10205.0
28	16187.0
29	24423.0
30	32827.0
31	42572.0
32	54803.0
33	72604.0
34	108596.0
35	199105.0
36	650146.0
37	3567384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8025419668774	21.91715279669588	14.509856723415758	23.770448513010958
2	14.458447573999036	20.51369520545157	41.68793858655634	23.339918633993054
3	18.219069216042506	25.588052144008717	30.819257636552162	25.373621003396625
4	13.041102124359524	18.00064086707018	38.67712094121871	30.281136067351582
5	13.701195206648316	37.03213833543651	35.36064429059459	13.90602216732058
6	30.053722195606408	38.05671151692401	18.504900963074995	13.384665324394597
7	26.766157626161576	32.711775775851	22.887930719555943	17.634135878431483
8	24.134323232892555	37.28775453329952	20.815675566786386	17.762246667021543
9	25.33721193599262	17.637705528887555	21.546076150455548	35.47900638466428
10-11	24.36605826045297	27.008319790580504	28.525692611345434	20.099929337621088
12-13	25.13257285166015	24.494680490190245	28.883962353756537	21.48878430439307
14-15	22.33832980021439	25.938207471842034	26.447321039958165	25.276141687985415
16-17	21.828255975250425	29.084499471336855	27.23499259454094	21.852251958871783
18-19	21.845874600893247	28.144094890076687	29.028825449311483	20.981205059718583
20-21	22.598429314398793	27.251398312245183	28.962658823919906	21.18751354943612
22-23	22.40626111005918	27.062554108532506	28.861666076217794	21.669518705190516
24-25	22.154790883689447	27.25703866572592	28.897312325821655	21.690858124762975
26-27	22.045969701246847	27.387953994803492	28.858480819614762	21.7075954843349
28-29	22.02477997651579	27.328955211660734	28.92779879604787	21.718466015775608
30-31	22.226146334284735	27.242551654391246	28.854633562258066	21.676668449065957
32-33	22.015433870533002	27.43287028650067	28.76227035012607	21.78942549284026
34-35	22.218567761780037	27.44676741219759	28.716686000430418	21.617978825591955
36-37	22.157444171872235	27.312041088153226	28.748103234617233	21.782411505357306
38-39	22.032167180244777	27.46967035246953	28.827748116188566	21.67041435109712
40-41	22.22937283684876	27.342039789990107	28.70833427054119	21.720253102619942
42-43	22.115704663689858	27.442123693927527	28.515868739656085	21.92630290272653
44-45	22.162133715556028	27.517397450729646	28.45657558692285	21.863893246791474
46-47	22.270181218751326	27.52795183482664	28.207736011714157	21.994130934707876
48-49	22.193961767502422	27.613576769847608	28.178773653539036	22.013687809110934
50-51	22.206688359910345	27.781495982953547	28.007594370983274	22.004221286152838
52-53	22.39197807088219	27.67898312241749	27.920891716276643	22.008147090423687
54-55	22.335319555290695	27.610189909854583	27.979012601835368	22.075477933019354
56-57	22.249362708518543	27.720148156458386	28.013775354965265	22.016713780057806
58-59	22.25060425620806	27.68256216745339	27.990304017636703	22.076529558701843
60-61	22.279778179833517	27.717992373333146	28.027130812533706	21.97509863429963
62-63	22.27131894573528	27.77569003799738	28.048070401991843	21.904920614275493
64-65	22.23785831320832	27.74621808635322	28.00878186564181	22.00714173479665
66-67	22.258183475310723	27.91380056135779	27.787966362001352	22.040049601330136
68-69	22.16266216067338	27.68500779534931	28.02760731661137	22.124722727365945
70-71	22.310965436218417	27.50759644212726	27.963467247589712	22.21797087406461
72-73	22.34147999207046	27.70064113323876	27.842045597774185	22.11583327691659
74-75	22.184349651953518	27.840357938102855	27.813571451787084	22.161720958156543
76-77	22.373589299084383	27.484447574554633	27.931714070872975	22.210249055488013
78-79	22.297823061021074	27.570274971892513	27.80510224372162	22.326799723364797
80-81	22.32199697087098	27.67714377344157	27.74314219190566	22.257717063781794
82-83	22.40674709614032	27.5902073325354	27.80895214085787	22.194093430466413
84-85	22.40872024819073	27.57959246769043	27.807149626761134	22.204537657357708
86-87	22.366609555318977	27.608197324544008	27.876372914408602	22.148820205728413
88-89	22.428156674289436	27.59363863599097	27.817929801875042	22.160274887844555
90-91	22.460459689468134	27.63278707527657	27.828665766346965	22.07808746890833
92-93	22.506142572192026	27.62795905310301	27.7723431023081	22.093555272396866
94-95	22.355673134809376	27.65289701722009	27.838055797466577	22.153374050503956
96-97	22.520552953601268	27.743032126576068	27.84969223209294	21.886722687729726
98-99	23.50856519764069	29.674735234961314	28.047419989799554	18.76927957759844
100	29.875378689336273	40.72527924404797	29.39934206661576	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	927.0
1	828.5
2	883.0
3	1120.0
4	1398.5
5	1895.5
6	2413.0
7	3140.0
8	4079.5
9	4806.0
10	5123.5
11	5319.0
12	5885.5
13	7286.0
14	10063.0
15	13201.5
16	15478.0
17	16842.5
18	17020.0
19	16023.5
20	14725.5
21	13821.0
22	14212.5
23	16554.5
24	20800.5
25	27024.5
26	34929.5
27	43918.5
28	54166.0
29	66048.5
30	77385.0
31	88400.5
32	101395.5
33	115278.5
34	127301.0
35	138397.5
36	150942.0
37	162249.0
38	171878.5
39	179368.0
40	186232.5
41	192136.0
42	194777.0
43	198451.0
44	202475.0
45	204452.5
46	204142.5
47	202922.0
48	201733.5
49	198834.5
50	194365.5
51	188635.5
52	180620.5
53	170357.0
54	158737.5
55	147195.5
56	134007.0
57	119734.0
58	106147.0
59	90346.0
60	74688.5
61	60629.0
62	47742.5
63	36915.5
64	27660.5
65	20471.0
66	15244.5
67	11717.0
68	9005.0
69	6687.0
70	4924.0
71	3440.5
72	2324.0
73	1517.0
74	981.5
75	636.5
76	399.0
77	250.5
78	162.5
79	105.5
80	76.5
81	58.5
82	39.0
83	24.5
84	18.5
85	15.0
86	11.0
87	6.5
88	4.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008851063119143868
3	7.515053591725926E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.131308281475389E-5
22-23	1.043810501213795E-5
24-25	1.6702094244280865E-4
26-27	2.1923494312680182E-4
28-29	0.0017857204395127927
30-31	0.0024979444216772662
32-33	0.0020419188130985635
34-35	3.7792553733137957E-4
36-37	1.0537584349411123E-5
38-39	5.298076120214619E-5
40-41	1.2792204601078832E-4
42-43	0.002091667376403375
44-45	1.5128664973120684E-4
46-47	4.358899112081353E-4
48-49	8.242157669399143E-4
50-51	0.0010635640140900075
52-53	0.0
54-55	0.002854826882848245
56-57	0.05177241521318433
58-59	0.0674337248105632
60-61	0.044350346315051685
62-63	0.007362031010175163
64-65	0.0
66-67	0.0
68-69	1.430774821796996E-4
70-71	0.001408623835913864
72-73	0.007267811942570673
74-75	0.011588963382554747
76-77	0.01294964901622903
78-79	0.0038392290928026714
80-81	0.0074168482236143096
82-83	0.043729830317791804
84-85	0.03651184424637517
86-87	0.008069477682232559
88-89	0.002090728133547824
90-91	0.0024060529379501547
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	139.0
22-23	329.0
24-25	343.0
26-27	1011.0
28-29	2646.0
30-31	8470.0
32-33	11149.0
34-35	15619.0
36-37	24985.0
38-39	28407.0
40-41	28367.0
42-43	32521.0
44-45	38102.0
46-47	39025.0
48-49	37350.0
50-51	33647.0
52-53	31481.0
54-55	33759.0
56-57	35393.0
58-59	36729.0
60-61	36258.0
62-63	35143.0
64-65	37001.0
66-67	38985.0
68-69	40427.0
70-71	39772.0
72-73	36885.0
74-75	37883.0
76-77	39928.0
78-79	41083.0
80-81	40742.0
82-83	37796.0
84-85	37749.0
86-87	38659.0
88-89	40733.0
90-91	41821.0
92-93	40751.0
94-95	46956.0
96-97	146515.0
98-99	1112572.0
100-101	2383220.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.5114197123484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.75121877064919	81.2103063372843
2	6.050179602685961	10.710199722968527
3	1.2953668318097549	3.4396427199530475
4	0.43494393419136534	1.5399002044220795
5	0.1962962870212844	0.8687231524258257
6	0.09617030982398302	0.5107302394018286
7	0.05144194255130823	0.3187239557584151
8	0.03681269569620448	0.2606675167607773
9	0.022830901601984407	0.18187159626941127
>10	0.0633345037695186	0.8352422354739184
>50	9.99920252338008E-4	0.06110464407235089
>100	4.042999471235715E-4	0.06288767520952408
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.17502977318107E-5	0.0	0.0	0.0
4	0.0	1.0437574432952675E-4	0.0	0.0	0.0
5	0.0	1.0437574432952675E-4	0.0	0.0	0.0
6	1.252508931954321E-4	1.0437574432952675E-4	0.0	2.087514886590535E-5	0.0
7	1.252508931954321E-4	1.0437574432952675E-4	0.0	2.087514886590535E-5	0.0
8	1.252508931954321E-4	1.252508931954321E-4	0.0	2.087514886590535E-5	0.0
9	1.252508931954321E-4	1.670011909272428E-4	0.0	2.087514886590535E-5	0.0
10-11	1.252508931954321E-4	1.670011909272428E-4	0.0	3.1312723298858024E-5	2.087514886590535E-5
12-13	1.252508931954321E-4	1.670011909272428E-4	0.0	6.262544659771605E-5	2.087514886590535E-5
14-15	1.252508931954321E-4	1.670011909272428E-4	0.0	6.262544659771605E-5	2.087514886590535E-5
16-17	1.8787633979314814E-4	1.670011909272428E-4	0.0	6.262544659771605E-5	2.087514886590535E-5
18-19	1.8787633979314814E-4	1.8787633979314817E-4	0.0	6.262544659771605E-5	2.087514886590535E-5
20-21	1.8787633979314814E-4	2.087514886590535E-4	0.0	6.262544659771605E-5	2.087514886590535E-5
22-23	1.8787633979314814E-4	2.7137693525676953E-4	0.0	6.262544659771605E-5	3.1312723298858024E-5
24-25	1.8787633979314814E-4	2.922520841226749E-4	0.0	7.306302103066872E-5	6.262544659771605E-5
26-27	1.8787633979314814E-4	2.922520841226749E-4	0.0	1.7743876536019547E-4	6.262544659771605E-5
28-29	1.8787633979314814E-4	2.922520841226749E-4	0.0	5.11441147214681E-4	6.262544659771605E-5
30-31	1.8787633979314814E-4	3.444399562874383E-4	0.0	0.0018787633979314815	6.262544659771605E-5
32-33	1.8787633979314814E-4	3.5487753072039094E-4	0.0	0.004947410281219568	6.262544659771605E-5
34-35	1.8787633979314814E-4	4.17502977318107E-4	0.0	0.009195503075431308	6.262544659771605E-5
36-37	1.8787633979314814E-4	5.010035727817284E-4	0.0	0.01615736522221074	6.262544659771605E-5
38-39	1.8787633979314814E-4	5.427538705135391E-4	0.0	0.031208347554528497	8.35005954636214E-5
40-41	1.8787633979314814E-4	6.471296148430659E-4	0.0	0.0470212728204518	8.35005954636214E-5
42-43	1.8787633979314814E-4	7.306302103066872E-4	0.0	0.058022476272783924	8.35005954636214E-5
44-45	1.8787633979314814E-4	8.454435290691667E-4	0.0	0.07102769401624295	8.35005954636214E-5
46-47	1.8787633979314814E-4	9.185065500998354E-4	0.0	0.08445041473702009	8.35005954636214E-5
48-49	1.8787633979314814E-4	9.185065500998354E-4	0.0	0.09805057422315742	8.35005954636214E-5
50-51	2.1918906309200618E-4	0.0010750701665941254	0.0	0.1110244792433176	8.35005954636214E-5
52-53	2.2962663752495885E-4	0.0010855077410270781	0.0	0.14071937850506797	9.393816989657408E-5
54-55	2.2962663752495885E-4	0.0011168204643259363	0.0	0.1783363967614294	1.1481331876247942E-4
56-57	2.505017863908642E-4	0.0011690083364906997	0.0	0.2301172035233076	1.252508931954321E-4
58-59	2.505017863908642E-4	0.0011690083364906997	0.0	0.2641541337491663	1.252508931954321E-4
60-61	2.7137693525676953E-4	0.0012107586342225104	0.0	0.2803323741202429	1.252508931954321E-4
62-63	3.5487753072039094E-4	0.001283821655253179	0.0	0.2933793421614338	1.252508931954321E-4
64-65	3.5487753072039094E-4	0.0015030107183451851	0.0	0.3002368285638837	1.252508931954321E-4
66-67	3.5487753072039094E-4	0.0015030107183451851	0.0	0.30619668356509966	1.252508931954321E-4
68-69	3.5487753072039094E-4	0.0015134482927781378	0.0	0.3122817894595111	1.252508931954321E-4
70-71	3.757526795862963E-4	0.0015447610160769958	0.0	0.31742751365495675	1.252508931954321E-4
72-73	4.592532750499177E-4	0.0015656361649429012	0.0	0.3217382318957662	1.670011909272428E-4
74-75	4.592532750499177E-4	0.0015760737393758538	0.0	0.32486950422565203	1.670011909272428E-4
76-77	4.592532750499177E-4	0.0016491367604065227	0.0	0.32783377536461056	1.670011909272428E-4
78-79	4.592532750499177E-4	0.0016804494837053807	0.0	0.3297542890602739	1.670011909272428E-4
80-81	4.592532750499177E-4	0.0016908870581383334	0.0	0.33047448169614757	1.670011909272428E-4
82-83	4.592532750499177E-4	0.0016908870581383334	0.0	0.33097548526892934	1.8787633979314814E-4
84-85	4.6969084948287037E-4	0.0016908870581383334	0.0	0.33115292403428953	1.8787633979314814E-4
86-87	4.8012842391582304E-4	0.0016908870581383334	0.0	0.331267737353052	1.8787633979314814E-4
88	4.8012842391582304E-4	0.0017535125047360494	0.0	0.3312886125019179	1.8787633979314814E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6290	0.0	49.26663	1
GTATCAA	11520	0.0	35.80472	1
TCAACGC	13390	0.0	30.641235	4
ATCAACG	13545	0.0	30.259047	3
CAACGCA	13725	0.0	29.924484	5
AACGCAG	14165	0.0	28.994955	6
TATCAAC	14205	0.0	28.886538	2
TGGTATC	3040	0.0	24.886576	2
ACGCAGA	16450	0.0	24.863466	7
CGCAGAG	16915	0.0	24.179958	8
GTGGTAT	3315	0.0	23.724648	1
GCAGAGT	19510	0.0	20.963814	9
GAGTACT	10450	0.0	20.162586	12-13
CAGAGTA	19255	0.0	18.500256	10-11
GTACTTT	11655	0.0	17.96808	14-15
AGAGTAC	17725	0.0	17.553381	10-11
GTACATG	14510	0.0	17.350555	1
TACATGG	14995	0.0	16.504316	2
ACATGGG	15025	0.0	15.985913	3
AGTACTT	11710	0.0	15.712016	12-13
>>END_MODULE