FastQCFastQC Report
Fri 27 May 2016
SRR2139480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470361
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.047.984591
GTATCAA106600.037.6077271
TCAACGC122900.032.0614244
ATCAACG124300.031.7346233
CAACGCA125550.031.452635
TATCAAC129300.030.7743952
AACGCAG129650.030.5237716
ACGCAGA148600.026.545187
CGCAGAG151700.026.0308348
TGGTATC28100.024.1322172
GTGGTAT29550.023.9583571
GCAGAGT179100.022.0484529
GAGTACT103750.020.24327912-13
GTACATG128800.019.2714141
CAGAGTA174800.019.01679810-11
TACATGG132550.018.9192542
AGAGTAC161400.018.2837510-11
GTACTTT115400.018.25508314-15
ACATGGG132350.018.1726343
GGACCGT5600.017.5146926
Produced by FastQC (version 0.11.5)