FastQCFastQC Report
Fri 27 May 2016
SRR2139479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444520
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.046.0762631
GTATCAA106200.035.857491
TCAACGC125600.030.3169464
CAACGCA127450.029.8768835
ATCAACG127650.029.7634123
TATCAAC131050.029.4152532
AACGCAG132000.028.9114976
CGAGACC33250.028.31881786-87
AGACCGA35850.027.05862488-89
GAGACCG35500.026.2197588-89
ACCGAGG37350.026.09298390-91
GACCGAG36850.025.98304490-91
ACGCAGA151200.025.2401967
ACGAGAC37100.025.08148286-87
CCCACGA37500.024.84404882-83
CGCAGAG154800.024.68078
TCCGAGC36900.024.51336976-77
TCTCCGA36700.024.44269274-75
ATCTCCG35350.024.42270974-75
CACGAGA38400.024.30956884-85
Produced by FastQC (version 0.11.5)