##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4444520
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.73594763889014	31.0	18.0	33.0	18.0	33.0
2	31.365658383807475	32.0	32.0	33.0	27.0	34.0
3	31.574988750191245	33.0	31.0	33.0	29.0	34.0
4	32.725432892640825	33.0	33.0	33.0	32.0	34.0
5	32.670114208058465	33.0	33.0	33.0	32.0	34.0
6	36.951461125160876	38.0	37.0	38.0	35.0	38.0
7	36.99543077767678	38.0	38.0	38.0	35.0	38.0
8	37.336861798349425	38.0	38.0	38.0	37.0	38.0
9	37.42757643120067	38.0	38.0	38.0	37.0	38.0
10-11	37.43905899849703	38.0	38.0	38.0	37.0	38.0
12-13	37.46963957862717	38.0	38.0	38.0	37.0	38.0
14-15	37.47879129804793	38.0	38.0	38.0	37.0	38.0
16-17	37.510855727952624	38.0	38.0	38.0	37.5	38.0
18-19	37.51041878538065	38.0	38.0	38.0	37.0	38.0
20-21	37.51633760400891	38.0	38.0	38.0	37.5	38.0
22-23	37.52659619549579	38.0	38.0	38.0	38.0	38.0
24-25	37.537759038663154	38.0	38.0	38.0	38.0	38.0
26-27	37.50734941491109	38.0	38.0	38.0	38.0	38.0
28-29	37.48173069497959	38.0	38.0	38.0	37.0	38.0
30-31	37.48333486079487	38.0	38.0	38.0	37.0	38.0
32-33	37.47376967053989	38.0	38.0	38.0	37.0	38.0
34-35	37.3719910784056	38.0	38.0	38.0	37.0	38.0
36-37	37.41581382250901	38.0	38.0	38.0	37.0	38.0
38-39	37.4230779941055	38.0	38.0	38.0	37.0	38.0
40-41	37.417271300677726	38.0	38.0	38.0	37.0	38.0
42-43	37.38336035593476	38.0	38.0	38.0	37.0	38.0
44-45	37.35285391458039	38.0	38.0	38.0	37.0	38.0
46-47	37.32431505466296	38.0	38.0	38.0	37.0	38.0
48-49	37.266824357672974	38.0	38.0	38.0	37.0	38.0
50-51	37.23575835116024	38.0	38.0	38.0	37.0	38.0
52-53	37.214216598671314	38.0	38.0	38.0	37.0	38.0
54-55	37.18415031665013	38.0	38.0	38.0	37.0	38.0
56-57	37.13642699681962	38.0	38.0	38.0	36.0	38.0
58-59	37.12927760241689	38.0	38.0	38.0	36.0	38.0
60-61	37.130329063151365	38.0	38.0	38.0	36.0	38.0
62-63	37.13544478988136	38.0	38.0	38.0	36.0	38.0
64-65	37.14205945954124	38.0	38.0	38.0	36.0	38.0
66-67	37.139215986022734	38.0	38.0	38.0	36.0	38.0
68-69	37.13019918335416	38.0	38.0	38.0	36.0	38.0
70-71	37.125315626224165	38.0	38.0	38.0	36.0	38.0
72-73	37.11257908435505	38.0	38.0	38.0	36.0	38.0
74-75	37.110908790673136	38.0	38.0	38.0	36.0	38.0
76-77	37.092686753655656	38.0	38.0	38.0	36.0	38.0
78-79	37.077634560329784	38.0	38.0	38.0	36.0	38.0
80-81	37.06718544918512	38.0	38.0	38.0	36.0	38.0
82-83	37.015497527602605	38.0	38.0	38.0	36.0	38.0
84-85	37.00213854339424	38.0	38.0	38.0	36.0	38.0
86-87	36.99086376313474	38.0	38.0	38.0	36.0	38.0
88-89	36.97491522587353	38.0	38.0	38.0	36.0	38.0
90-91	36.96301741773654	38.0	38.0	38.0	36.0	38.0
92-93	36.943277501683134	38.0	38.0	38.0	35.0	38.0
94-95	36.92021020849335	38.0	38.0	38.0	35.0	38.0
96-97	36.904956043511504	38.0	38.0	38.0	35.0	38.0
98-99	36.9189116738263	38.0	38.0	38.0	35.0	38.0
100	35.49322103853579	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	8.0
21	41.0
22	201.0
23	662.0
24	1562.0
25	3254.0
26	6035.0
27	10102.0
28	16049.0
29	23535.0
30	31631.0
31	40675.0
32	52060.0
33	69910.0
34	104423.0
35	189204.0
36	613509.0
37	3281652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.804050831135875	22.207842466678066	14.430534680910426	23.55757202127564
2	14.444666962190233	20.745506954179596	41.72361495829293	23.086211125337243
3	18.195023501009114	25.991418587959476	30.844573843118106	24.968984067913304
4	13.069870312204692	18.175371018692683	38.882488997687034	29.872269671415587
5	13.652160413272973	37.37379964540603	35.332791842538676	13.641248098782322
6	29.90410663018729	38.499658005813906	18.494168099142314	13.102067264856498
7	26.640852105514206	33.12015695733172	22.952377309585735	17.286613627568332
8	24.134237217967293	37.56666636667177	20.807758768100943	17.491337647259996
9	25.479916841413697	17.823139506628387	21.645442027485533	35.05150162447239
10-11	24.345621124440882	27.34177143988552	28.447841386696425	19.864766048977167
12-13	25.128877809077245	24.811363206825483	28.829929891191853	21.22982909290542
14-15	22.312308190760756	26.26155355358959	26.507463123126907	24.918675132522747
16-17	21.76827868926228	29.420972343470158	27.19767263956513	21.61307632770243
18-19	21.768312438688543	28.490556910532522	29.023527850026547	20.71760280075239
20-21	22.641828042126257	27.548533012753662	28.963702812204616	20.84593613291546
22-23	22.37487220779589	27.38778052828766	28.84521074702201	21.392136516894446
24-25	22.112629196360693	27.589673899896383	28.90492522782249	21.392771675920436
26-27	21.991606831611005	27.728176394948168	28.84701794730959	21.43319882613124
28-29	21.988482782935716	27.76166907340391	28.889492261743143	21.360355881917233
30-31	22.255709147685003	27.646346666077708	28.76038854048514	21.337555645752147
32-33	22.00023736770731	27.846416543738727	28.6910593793049	21.462286709249064
34-35	22.15135617243155	27.872311560679762	28.70147491877098	21.274857348117703
36-37	22.135935358010567	27.71892038188855	28.724917312633586	21.420226947467295
38-39	21.97985164055012	27.90073804386335	28.79896452317807	21.320445792408456
40-41	22.24943931709248	27.73986893857017	28.66182450469457	21.34886723964278
42-43	22.124676773545644	27.906404478113416	28.407092233469278	21.561826514871665
44-45	22.160279153228966	27.98133285606834	28.326028137578653	21.53235985312404
46-47	22.24257735040535	27.994844486925626	28.094996125218945	21.667582037450078
48-49	22.21346694203307	28.106161015480126	28.033963709535705	21.6464083329511
50-51	22.169677928235696	28.2233767064126	27.950800176766123	21.656145188585583
52-53	22.34128239255057	28.174421803549183	27.828111441966907	21.656184361933335
54-55	22.35447358745842	28.101302720229832	27.85562367834758	21.68860001396417
56-57	22.265374399867998	28.205717677219795	27.837271414199748	21.69163650871246
58-59	22.223138334837067	28.166082097273552	27.781715684408653	21.829063883480725
60-61	22.415252169652533	28.18434830053817	27.969882136584683	21.430517393224616
62-63	22.183243702171946	28.280671014984975	27.906546851969978	21.6295384308731
64-65	22.13217276482534	28.2814707771559	27.8455595397815	21.740796918237262
66-67	22.241537728935292	28.60253127203733	27.830467429000887	21.32546357002649
68-69	22.13581691615994	28.40369609638518	27.840963831115918	21.61952315633896
70-71	22.1982567738062	28.211240179950316	27.62871910418835	21.961783942055142
72-73	22.28448414618765	28.474049122199663	27.474540966918166	21.76692576469452
74-75	22.13127395438422	28.524778845213888	27.70286162080468	21.64108557959721
76-77	22.24785169524639	27.78485356878792	27.896695752808192	22.070598983157495
78-79	22.197064281731965	27.86650335072235	27.583282840762337	22.35314952678335
80-81	22.46034394661352	28.192595155224424	27.500849431543788	21.846211466618268
82-83	22.531723365528798	27.73920088598426	27.687174872143817	22.041900876343128
84-85	22.301454904037975	27.757197678149325	27.628159190322087	22.313188227490617
86-87	22.179559590075808	28.04607791100163	27.69085053826367	22.083511960658893
88-89	22.50362784701985	28.025664536887255	27.62246374911391	21.84824386697899
90-91	22.547414280372532	28.0332820276471	27.622488702603835	21.796814989376536
92-93	22.374002711141987	27.797620845356708	27.57843648095764	22.249939962543664
94-95	22.432964263231373	28.051013790734224	27.64333778131929	21.87268416471511
96-97	22.82479728457581	27.87972275949541	27.72695979849881	21.568520157429965
98-99	23.465905036487413	29.9463736151509	28.04920481855146	18.53851652981022
100	29.738829755522637	40.91187166658259	29.34929857789477	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1040.0
1	986.0
2	932.0
3	1009.0
4	1316.0
5	1842.5
6	2276.5
7	2739.5
8	3318.0
9	3775.5
10	4059.5
11	4353.5
12	5042.5
13	6494.5
14	9257.0
15	12553.0
16	15174.0
17	16796.0
18	17081.5
19	16069.5
20	14586.5
21	13869.5
22	14592.0
23	16929.0
24	21285.0
25	27868.0
26	36449.5
27	45943.5
28	55836.5
29	67002.0
30	77607.5
31	88147.5
32	100159.0
33	112635.0
34	123836.0
35	134041.5
36	145845.0
37	155721.5
38	162549.0
39	168320.5
40	174062.5
41	178699.5
42	181242.5
43	183910.0
44	187082.5
45	189315.5
46	189125.5
47	188004.5
48	186519.0
49	183168.5
50	178901.0
51	174002.0
52	166518.5
53	156762.0
54	145478.0
55	133854.0
56	120749.5
57	106873.0
58	94317.0
59	80899.0
60	67069.0
61	53941.5
62	42364.5
63	32508.5
64	24267.0
65	18016.5
66	13303.0
67	10166.0
68	7753.0
69	5744.5
70	4230.0
71	3000.5
72	2173.0
73	1530.5
74	979.0
75	627.0
76	419.5
77	263.5
78	176.0
79	117.0
80	81.5
81	53.5
82	30.0
83	21.0
84	18.5
85	14.0
86	11.0
87	10.0
88	7.5
89	3.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009674835527796029
3	6.749885251950717E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.1249987203139556E-5
22-23	1.1250550433179942E-5
24-25	7.876019747656829E-5
26-27	1.1252995125315042E-4
28-29	0.0013845864459793467
30-31	0.001994451818156926
32-33	0.002032603180865887
34-35	3.736996526065381E-4
36-37	1.1371821490604715E-5
38-39	1.144324886651759E-5
40-41	5.760634794303285E-5
42-43	0.0014618068164283882
44-45	2.1055764085604314E-4
46-47	1.7709205634691436E-4
48-49	8.816094948866054E-4
50-51	0.0013099846791883576
52-53	0.0
54-55	0.0027097565149504102
56-57	0.05524502194848171
58-59	0.07307301515639396
60-61	0.04741340598149867
62-63	0.007272814207908117
64-65	0.0
66-67	0.0
68-69	1.551827347827487E-4
70-71	0.0013846963360934948
72-73	0.0069391730036091614
74-75	0.011319158728163345
76-77	0.01361020873245517
78-79	0.0040501148710432545
80-81	0.0076659355635527344
82-83	0.04787711909711085
84-85	0.03963337514595955
86-87	0.00924240096030247
88-89	0.0023786245904216224
90-91	0.002855065429548942
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	168.0
22-23	364.0
24-25	492.0
26-27	1146.0
28-29	2884.0
30-31	8943.0
32-33	11633.0
34-35	16104.0
36-37	25998.0
38-39	29753.0
40-41	29328.0
42-43	33706.0
44-45	38873.0
46-47	38686.0
48-49	37136.0
50-51	34265.0
52-53	30722.0
54-55	32965.0
56-57	35078.0
58-59	18845.0
60-61	35200.0
62-63	33837.0
64-65	35432.0
66-67	37004.0
68-69	38611.0
70-71	38348.0
72-73	35389.0
74-75	36274.0
76-77	38428.0
78-79	39503.0
80-81	39138.0
82-83	37226.0
84-85	36561.0
86-87	37445.0
88-89	38931.0
90-91	39958.0
92-93	39025.0
94-95	44536.0
96-97	152427.0
98-99	1003546.0
100-101	2180570.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.74334401343069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.49924787837804	80.28449983562692
2	6.087639302228009	10.682996590501466
3	1.3948517641735012	3.6716687457484856
4	0.4780759190801181	1.6779191932953539
5	0.21006415642242732	0.9215865770932077
6	0.11174061720078479	0.5882697249192917
7	0.06637678269176239	0.4076884614759627
8	0.03908427399377638	0.2743507918840883
9	0.027869947834964798	0.22008621784677151
>10	0.08300293176933274	1.0920496191640494
>50	0.0014798217678048274	0.08532353521325856
>100	5.666044594832315E-4	0.0935607072311766
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.249961750650239E-5	0.0	0.0	0.0
3	0.0	2.249961750650239E-5	0.0	2.249961750650239E-5	0.0
4	0.0	6.749885251950717E-5	0.0	2.249961750650239E-5	0.0
5	0.0	8.999847002600956E-5	0.0	2.249961750650239E-5	0.0
6	6.749885251950717E-5	8.999847002600956E-5	0.0	2.249961750650239E-5	0.0
7	6.749885251950717E-5	8.999847002600956E-5	0.0	2.249961750650239E-5	0.0
8	6.749885251950717E-5	1.1249808753251195E-4	0.0	2.249961750650239E-5	0.0
9	6.749885251950717E-5	1.3499770503901435E-4	0.0	2.249961750650239E-5	2.249961750650239E-5
10-11	8.999847002600956E-5	1.3499770503901435E-4	0.0	7.874866127275837E-5	2.249961750650239E-5
12-13	1.1249808753251195E-4	1.3499770503901435E-4	0.0	1.3499770503901435E-4	2.249961750650239E-5
14-15	1.1249808753251195E-4	2.137463663117727E-4	0.0	1.5749732254551673E-4	3.374942625975359E-5
16-17	1.1249808753251195E-4	2.249961750650239E-4	0.0	2.024965575585215E-4	4.499923501300478E-5
18-19	1.1249808753251195E-4	2.249961750650239E-4	0.0	2.474957925715263E-4	4.499923501300478E-5
20-21	1.1249808753251195E-4	2.249961750650239E-4	0.0	2.9249502758453105E-4	4.499923501300478E-5
22-23	1.1249808753251195E-4	2.362459838182751E-4	0.0	3.599938801040382E-4	5.6249043766255976E-5
24-25	1.1249808753251195E-4	2.9249502758453105E-4	0.0	4.162429238702942E-4	8.999847002600956E-5
26-27	1.1249808753251195E-4	3.1499464509103346E-4	0.0	5.17491202649555E-4	8.999847002600956E-5
28-29	1.1249808753251195E-4	3.1499464509103346E-4	0.0	7.537371864678301E-4	8.999847002600956E-5
30-31	1.1249808753251195E-4	3.374942625975358E-4	0.0	0.0017099709304941816	8.999847002600956E-5
32-33	1.1249808753251195E-4	3.599938801040382E-4	0.0	0.00404993115117043	8.999847002600956E-5
34-35	1.3499770503901435E-4	3.824934976105406E-4	0.0	0.0069636316182624895	1.1249808753251195E-4
36-37	1.3499770503901435E-4	4.162429238702942E-4	0.0	0.01234104020231656	1.1249808753251195E-4
38-39	1.3499770503901435E-4	4.274927326235454E-4	0.0	0.027269536417880897	1.1249808753251195E-4
40-41	1.3499770503901435E-4	4.7249196763655016E-4	0.0	0.043424261787549615	1.1249808753251195E-4
42-43	1.5749732254551673E-4	4.837417763898014E-4	0.0	0.051816619117475	1.1249808753251195E-4
44-45	1.799969400520191E-4	5.737402464158109E-4	0.0	0.0625826860943364	1.1249808753251195E-4
46-47	2.137463663117727E-4	6.524889076885693E-4	0.0	0.07394499293512011	1.2374789628576314E-4
48-49	2.474957925715263E-4	6.524889076885693E-4	0.0	0.0852623005408908	1.3499770503901435E-4
50-51	2.474957925715263E-4	8.09986230234086E-4	0.0	0.09668085642544078	1.3499770503901435E-4
52-53	2.474957925715263E-4	8.09986230234086E-4	0.0	0.12930530180986924	1.3499770503901435E-4
54-55	2.699954100780287E-4	8.09986230234086E-4	0.0	0.17019835662793734	1.3499770503901435E-4
56-57	2.699954100780287E-4	8.09986230234086E-4	0.0	0.2217224807178278	1.3499770503901435E-4
58-59	2.699954100780287E-4	8.09986230234086E-4	0.0	0.4475623914393455	1.3499770503901435E-4
60-61	2.699954100780287E-4	8.437356564938396E-4	0.0	0.6527139038636343	1.3499770503901435E-4
62-63	3.1499464509103346E-4	8.66235274000342E-4	0.0	0.6602062764932997	1.3499770503901435E-4
64-65	3.1499464509103346E-4	9.674835527796028E-4	0.0	0.6669674115540036	1.3499770503901435E-4
66-67	3.712436888572894E-4	9.674835527796028E-4	0.0	0.6734585512046295	1.3499770503901435E-4
68-69	4.04993115117043E-4	9.674835527796028E-4	0.0	0.6801071881778009	1.3499770503901435E-4
70-71	4.04993115117043E-4	9.674835527796028E-4	0.0	0.6854283477180888	1.3499770503901435E-4
72-73	4.274927326235454E-4	9.674835527796028E-4	0.0	0.6892757823117007	1.799969400520191E-4
74-75	4.274927326235454E-4	0.00103498240529911	0.0	0.6924032291451045	1.799969400520191E-4
76-77	4.274927326235454E-4	0.0010912314490653659	0.0	0.6958569204323526	1.799969400520191E-4
78-79	4.499923501300478E-4	0.0011024812578186171	0.0	0.6981518814180159	1.799969400520191E-4
80-81	4.499923501300478E-4	0.0011024812578186171	0.0	0.6989281182219902	1.799969400520191E-4
82-83	4.499923501300478E-4	0.0011024812578186171	0.0	0.6995693573209256	2.024965575585215E-4
84-85	4.499923501300478E-4	0.0011249808753251195	0.0	0.6998618523485101	2.024965575585215E-4
86-87	4.499923501300478E-4	0.0011249808753251195	0.0	0.6998843519660165	2.024965575585215E-4
88	4.499923501300478E-4	0.0011699801103381242	0.0	0.6998956017747698	2.024965575585215E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6030	0.0	46.076263	1
GTATCAA	10620	0.0	35.85749	1
TCAACGC	12560	0.0	30.316946	4
CAACGCA	12745	0.0	29.876883	5
ATCAACG	12765	0.0	29.763412	3
TATCAAC	13105	0.0	29.415253	2
AACGCAG	13200	0.0	28.911497	6
CGAGACC	3325	0.0	28.318817	86-87
AGACCGA	3585	0.0	27.058624	88-89
GAGACCG	3550	0.0	26.21975	88-89
ACCGAGG	3735	0.0	26.092983	90-91
GACCGAG	3685	0.0	25.983044	90-91
ACGCAGA	15120	0.0	25.240196	7
ACGAGAC	3710	0.0	25.081482	86-87
CCCACGA	3750	0.0	24.844048	82-83
CGCAGAG	15480	0.0	24.6807	8
TCCGAGC	3690	0.0	24.513369	76-77
TCTCCGA	3670	0.0	24.442692	74-75
ATCTCCG	3535	0.0	24.422709	74-75
CACGAGA	3840	0.0	24.309568	84-85
>>END_MODULE