FastQCFastQC Report
Fri 27 May 2016
SRR2139478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4717318
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT44600.062.55705394
GGTATCA58200.047.987991
TATCTCG55200.044.597994
GTATCAA107700.038.0339361
TCAACGC128700.031.5221234
ATCAACG129750.031.1672533
TATCAAC132600.030.8918172
TGCCGTC25000.030.67518888-89
CAACGCA132550.030.6065435
ATGCCGT26700.030.15818888-89
GCCGTCT27350.030.10764390-91
TATGCCG25500.029.95492486-87
AACGCAG136950.029.749666
CCGTCTT26250.029.67366290-91
CTCGTAT25250.028.96707582-83
TCTCGTA25450.028.73943582-83
TCGTATG28150.028.08183384-85
CTATCTC69050.026.824592-93
CGTATGC28250.026.65444884-85
ACGCAGA158300.025.6826067
Produced by FastQC (version 0.11.5)