##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4717318
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.397500232123424	30.0	18.0	33.0	18.0	33.0
2	31.215768154701465	32.0	31.0	33.0	27.0	34.0
3	31.486700705782397	33.0	31.0	33.0	29.0	34.0
4	32.740819253652184	33.0	33.0	33.0	32.0	34.0
5	32.67282404959767	33.0	33.0	33.0	32.0	34.0
6	36.96206000952236	38.0	37.0	38.0	35.0	38.0
7	37.01107027340535	38.0	38.0	38.0	35.0	38.0
8	37.345634532164254	38.0	38.0	38.0	37.0	38.0
9	37.43698326040347	38.0	38.0	38.0	37.0	38.0
10-11	37.45222052021933	38.0	38.0	38.0	37.0	38.0
12-13	37.480246084745616	38.0	38.0	38.0	37.0	38.0
14-15	37.49033009858568	38.0	38.0	38.0	37.0	38.0
16-17	37.520557867839315	38.0	38.0	38.0	37.5	38.0
18-19	37.52544507281468	38.0	38.0	38.0	37.5	38.0
20-21	37.53290446014644	38.0	38.0	38.0	38.0	38.0
22-23	37.54313181153328	38.0	38.0	38.0	38.0	38.0
24-25	37.553704289856356	38.0	38.0	38.0	38.0	38.0
26-27	37.525899362157446	38.0	38.0	38.0	38.0	38.0
28-29	37.501829907475816	38.0	38.0	38.0	37.0	38.0
30-31	37.50420251878728	38.0	38.0	38.0	38.0	38.0
32-33	37.49372180231804	38.0	38.0	38.0	38.0	38.0
34-35	37.396348287328294	38.0	38.0	38.0	37.0	38.0
36-37	37.43696636453368	38.0	38.0	38.0	37.0	38.0
38-39	37.44126341598025	38.0	38.0	38.0	37.0	38.0
40-41	37.43789212062721	38.0	38.0	38.0	37.0	38.0
42-43	37.405520315806726	38.0	38.0	38.0	37.0	38.0
44-45	37.37598938590659	38.0	38.0	38.0	37.0	38.0
46-47	37.34584865208865	38.0	38.0	38.0	37.0	38.0
48-49	37.286971526047225	38.0	38.0	38.0	37.0	38.0
50-51	37.25321045150078	38.0	38.0	38.0	37.0	38.0
52-53	37.230027542095144	38.0	38.0	38.0	37.0	38.0
54-55	37.203316772402886	38.0	38.0	38.0	37.0	38.0
56-57	37.15759036115652	38.0	38.0	38.0	36.5	38.0
58-59	37.15112992122039	38.0	38.0	38.0	36.0	38.0
60-61	37.1486857628702	38.0	38.0	38.0	36.0	38.0
62-63	37.1558535864985	38.0	38.0	38.0	36.0	38.0
64-65	37.15750446620994	38.0	38.0	38.0	36.0	38.0
66-67	37.15276371984501	38.0	38.0	38.0	36.0	38.0
68-69	37.14146063972485	38.0	38.0	38.0	36.0	38.0
70-71	37.140033020501775	38.0	38.0	38.0	36.0	38.0
72-73	37.129502894689345	38.0	38.0	38.0	36.0	38.0
74-75	37.11618541022885	38.0	38.0	38.0	36.0	38.0
76-77	37.09636792607435	38.0	38.0	38.0	36.0	38.0
78-79	37.080840892967586	38.0	38.0	38.0	36.0	38.0
80-81	37.064999396858724	38.0	38.0	38.0	36.0	38.0
82-83	36.992090155852196	38.0	38.0	38.0	36.0	38.0
84-85	37.00645189676193	38.0	38.0	38.0	36.0	38.0
86-87	36.99639039166631	38.0	38.0	38.0	36.0	38.0
88-89	36.9776794141646	38.0	38.0	38.0	36.0	38.0
90-91	36.9749711438111	38.0	38.0	38.0	36.0	38.0
92-93	36.952604340224234	38.0	38.0	38.0	35.0	38.0
94-95	36.89873520635787	38.0	38.0	38.0	35.0	38.0
96-97	36.83576027627824	38.0	38.0	38.0	35.0	38.0
98-99	36.87039213689703	38.0	38.0	38.0	35.0	38.0
100	35.453037291998044	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	6.0
21	62.0
22	182.0
23	657.0
24	1527.0
25	3299.0
26	5973.0
27	10145.0
28	16346.0
29	24208.0
30	32131.0
31	41411.0
32	53624.0
33	71981.0
34	107487.0
35	200793.0
36	657087.0
37	3490395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.70158467162909	22.082293370936622	14.372297987966892	23.843823969467394
2	14.508983749748245	20.540339421435917	41.50594146517273	23.44473536364311
3	18.294176916047117	25.564006523254832	30.748671320340105	25.393145240357946
4	13.16154645499837	17.95764033715768	38.54751789046234	30.333295317381616
5	13.883906066964322	37.08634864132543	35.16468467887897	13.865060612831273
6	30.385062020410754	37.94280987628987	18.39591903704605	13.276209066253324
7	27.03881739581686	32.75306010745936	22.698045796361406	17.51007670036237
8	24.448701571528566	37.15157638302103	20.719336707849674	17.68038533760073
9	25.70180767970275	17.540263344552987	21.30644997856833	35.45147899717594
10-11	24.54697775303679	26.99614484331987	28.363415398325913	20.093462005317427
12-13	25.353643744178367	24.450386851172638	28.647178333112162	21.548791071536836
14-15	22.510979755869755	25.878571255955187	26.285571589619355	25.324877398555707
16-17	22.052255116148626	29.0212468186372	26.96829003259903	21.958208032615143
18-19	22.051248188059404	28.133093846969825	28.748825073908524	21.06683289106225
20-21	22.880221976740266	27.215734333407315	28.663123925353982	21.240919764498432
22-23	22.629416183604146	27.054183031539168	28.555368255873205	21.76103252898349
24-25	22.37083730106442	27.251149188844355	28.61555751997187	21.762455990119356
26-27	22.268170689118865	27.38688715145586	28.54746230667241	21.79747985275287
28-29	22.204501711929975	27.42864554543985	28.612085279608156	21.754767463022016
30-31	22.491406672965255	27.289508603942636	28.508561480239063	21.710523242853046
32-33	22.271253322576843	27.440261695874707	28.452309986048046	21.8361749955004
34-35	22.393805757123666	27.51893891721911	28.40852514715337	21.678730178503855
36-37	22.41955586224296	27.31999007549286	28.434334040192113	21.82612002207207
38-39	22.21297786826369	27.45336489345019	28.454121484363824	21.8795357539223
40-41	22.580963874280123	27.298107560375644	28.47030499466215	21.650623570682082
42-43	22.30009352317977	27.402204622623906	28.2601696256951	22.03753222850123
44-45	22.362559500496268	27.450501595817233	28.21742368388389	21.969515219802606
46-47	22.417339420600584	27.46526138630595	28.123112753220127	21.99428643987334
48-49	22.374447970008926	27.70955720015788	27.920853073060847	21.995141756772345
50-51	22.335359311137555	27.832964803321943	27.685582791035614	22.14609309450489
52-53	22.383733843419876	27.641002317352093	27.61091078904745	22.364353050180583
54-55	22.565521722870315	27.363872814273844	27.930893852637258	22.139711610218583
56-57	22.3623095833307	27.10298559243402	28.08365233198024	22.45105249225504
58-59	22.176421767901804	26.909575701577577	28.142515142214087	22.771487388306536
60-61	22.41906054690965	27.232519166129503	28.392824568673948	21.955595718286897
62-63	21.95131932149115	27.483971144277007	28.38582138328561	22.178888150946232
64-65	21.76827555920235	27.791932231204385	28.038137282660824	22.401654926932434
66-67	21.878253298142575	28.611266600309925	27.431081234384607	22.07939886716289
68-69	21.911720487241997	28.3468995429679	27.441049802788324	22.30033016700178
70-71	22.03028426598734	27.76159116989545	27.35791424120062	22.85021032291659
72-73	22.186781869474277	27.43310045234538	27.336320976264616	23.043796701915735
74-75	22.22782081104424	27.45566656150144	27.52021847894278	22.79629414851154
76-77	22.527141830430942	27.077635185495907	27.08612855986955	23.3090944242036
78-79	22.702941334010653	27.20753089720055	26.548118275389587	23.54140949339921
80-81	22.53106736517295	27.64925983114596	26.712628290552047	23.10704451312904
82-83	22.57445779136204	27.279713582824648	26.815630348535645	23.330198277277667
84-85	22.57693268933685	27.684926298236746	26.769539658567847	22.96860135385856
86-87	23.10306123524032	27.586912217831284	26.762495313623013	22.547531233305385
88-89	22.806510389860392	27.770924746446855	27.13664110831408	22.28592375537867
90-91	22.785488897719596	27.34140293096633	26.99033422591129	22.882773945402786
92-93	22.844590665508434	26.884801281168297	27.168175367607343	23.102432685715925
94-95	22.04565588886561	27.373053086079924	27.839790355420625	22.74150066963384
96-97	22.23329996375797	27.2507351351086	27.98884269022226	22.52712221091117
98-99	23.45175367408862	29.087145623651733	28.294450989060337	19.16664971319931
100	30.11028537228932	40.352357870446006	29.53735675726467	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	935.0
1	877.0
2	874.0
3	1021.5
4	1291.5
5	1745.5
6	2198.5
7	2789.5
8	3548.5
9	4069.0
10	4296.0
11	4554.5
12	5171.0
13	6480.0
14	9214.0
15	12549.5
16	15005.0
17	16343.0
18	16450.0
19	15377.5
20	13890.0
21	12785.0
22	12883.5
23	14778.0
24	18529.0
25	24042.5
26	31358.5
27	40173.5
28	49222.5
29	59457.5
30	70014.5
31	80390.0
32	92198.0
33	104834.0
34	116468.5
35	127359.5
36	139928.5
37	150879.0
38	159281.5
39	166936.5
40	175142.0
41	181950.0
42	185967.0
43	190684.5
44	196783.0
45	201387.5
46	202892.0
47	203433.5
48	203182.5
49	201033.0
50	197310.0
51	192059.5
52	183694.0
53	172938.0
54	161262.5
55	148618.5
56	134699.5
57	120125.0
58	105391.5
59	90010.0
60	74427.5
61	59278.5
62	46323.0
63	35463.5
64	26265.5
65	19443.0
66	14439.5
67	11101.0
68	8463.5
69	6220.0
70	4527.5
71	3174.0
72	2251.5
73	1540.0
74	964.0
75	633.0
76	414.5
77	252.5
78	159.0
79	103.5
80	70.5
81	45.5
82	25.0
83	13.5
84	6.0
85	3.0
86	4.0
87	5.5
88	5.5
89	4.5
90	3.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009920891489613377
3	8.691379296456165E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.2996939850699144E-5
22-23	1.0599867713650935E-5
24-25	1.5900934211686803E-4
26-27	2.438422154273881E-4
28-29	0.0020362028380425472
30-31	0.0026856743054108484
32-33	0.002255067732874037
34-35	4.3728794867476685E-4
36-37	0.0
38-39	2.1512389845807796E-5
40-41	1.6214584240224037E-4
42-43	0.001991204317366196
44-45	2.302603048887662E-4
46-47	3.9818674604445714E-4
48-49	0.0011936847161836094
50-51	0.0011809316651080243
52-53	0.0
54-55	0.0029669546680914064
56-57	0.052475094083249726
58-59	0.06778264470668591
60-61	0.04432185235560017
62-63	0.006900064599275883
64-65	0.0
66-67	0.0
68-69	1.0591694416906039E-4
70-71	0.001390290071546466
72-73	0.0065019295255618805
74-75	0.011173061277159588
76-77	0.012145883052741847
78-79	0.0037147583895226536
80-81	0.007132026401963751
82-83	0.044139304349951114
84-85	0.03668598829304828
86-87	0.007836781174458568
88-89	0.002063871501023888
90-91	0.0023359414513335467
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	159.0
22-23	350.0
24-25	416.0
26-27	1141.0
28-29	2843.0
30-31	9267.0
32-33	11472.0
34-35	16381.0
36-37	26193.0
38-39	16235.0
40-41	29768.0
42-43	33414.0
44-45	39097.0
46-47	39071.0
48-49	37185.0
50-51	18793.0
52-53	2689.0
54-55	2521.0
56-57	2310.0
58-59	19944.0
60-61	36958.0
62-63	35392.0
64-65	37424.0
66-67	39673.0
68-69	40534.0
70-71	40580.0
72-73	37593.0
74-75	38022.0
76-77	40137.0
78-79	41273.0
80-81	41222.0
82-83	39055.0
84-85	38418.0
86-87	39499.0
88-89	41498.0
90-91	42589.0
92-93	41546.0
94-95	48034.0
96-97	163043.0
98-99	1162942.0
100-101	2362598.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.93486475012753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.76770613685055	78.90878256686423
2	6.733190879723599	11.706980769311265
3	1.4416177722305599	3.759805381507316
4	0.48607664030455805	1.6902802793229255
5	0.22590257649143225	0.9819404966994001
6	0.12159215834783295	0.6342358704386968
7	0.06204726006995819	0.37758491136084626
8	0.04020131452253117	0.27959166726348683
9	0.027252219038159605	0.21322511786009304
>10	0.09195931540026167	1.2377028448142997
>50	0.0017945768192204007	0.10080996076089044
>100	6.230276250802102E-4	0.0931387556182291
>500	3.6122576216013134E-5	0.015921378178269947
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1198486088917476E-5	2.1198486088917476E-5
2	0.0	4.239697217783495E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
3	0.0	4.239697217783495E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
4	0.0	8.47939443556699E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
5	0.0	8.47939443556699E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
6	0.0	8.47939443556699E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
7	0.0	8.47939443556699E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
8	0.0	8.47939443556699E-5	0.0	4.239697217783495E-5	2.1198486088917476E-5
9	0.0	2.331833469780922E-4	0.0	6.359545826675242E-5	2.1198486088917476E-5
10-11	4.239697217783495E-5	2.331833469780922E-4	0.0	6.359545826675242E-5	2.1198486088917476E-5
12-13	6.359545826675242E-5	2.331833469780922E-4	0.0	7.419470131121116E-5	4.239697217783495E-5
14-15	6.359545826675242E-5	2.7558031915592715E-4	0.0	1.0599243044458737E-4	4.239697217783495E-5
16-17	6.359545826675242E-5	2.7558031915592715E-4	0.0	1.2719091653350483E-4	4.239697217783495E-5
18-19	6.359545826675242E-5	2.7558031915592715E-4	0.0	1.2719091653350483E-4	4.239697217783495E-5
20-21	6.359545826675242E-5	2.7558031915592715E-4	0.0	1.2719091653350483E-4	4.239697217783495E-5
22-23	6.359545826675242E-5	2.9677880524484464E-4	0.0	1.5898864566688106E-4	4.239697217783495E-5
24-25	6.359545826675242E-5	3.603742635115971E-4	0.0	1.9078637480025726E-4	4.239697217783495E-5
26-27	6.359545826675242E-5	4.02771235689432E-4	0.0	2.2258410393363346E-4	5.299621522229369E-5
28-29	6.359545826675242E-5	4.02771235689432E-4	0.0	5.723591244007718E-4	6.359545826675242E-5
30-31	6.359545826675242E-5	4.4516820786726693E-4	0.0	0.0017594743453801503	6.359545826675242E-5
32-33	6.359545826675242E-5	4.4516820786726693E-4	0.0	0.004377487377361458	1.0599243044458737E-4
34-35	6.359545826675242E-5	5.193629091784782E-4	0.0	0.00797063076943297	1.0599243044458737E-4
36-37	6.359545826675242E-5	5.935576104896893E-4	0.0	0.016397028989777667	1.0599243044458737E-4
38-39	6.359545826675242E-5	6.147560965786067E-4	0.0	0.18248716749644606	1.0599243044458737E-4
40-41	6.359545826675242E-5	6.783515548453592E-4	0.0	0.3532939691578986	1.0599243044458737E-4
42-43	6.359545826675242E-5	6.889507978898179E-4	0.0	0.372595190741858	1.0599243044458737E-4
44-45	7.419470131121116E-5	8.161417144233227E-4	0.0	0.3976094043267806	1.0599243044458737E-4
46-47	8.47939443556699E-5	9.115349018234514E-4	0.0	0.4240651149657496	1.165916734890461E-4
48-49	8.47939443556699E-5	9.115349018234514E-4	0.0	0.4509659938125859	1.2719091653350483E-4
50-51	8.47939443556699E-5	9.539318740012864E-4	0.0	0.47874660983211226	1.2719091653350483E-4
52-53	8.47939443556699E-5	9.539318740012864E-4	0.0	0.9938062263345401	1.2719091653350483E-4
54-55	8.47939443556699E-5	9.539318740012864E-4	0.0	1.6665401823663362	1.2719091653350483E-4
56-57	8.47939443556699E-5	9.539318740012864E-4	0.0	2.4053180218081547	1.2719091653350483E-4
58-59	8.47939443556699E-5	9.645311170457451E-4	0.0	3.174621681218014	1.2719091653350483E-4
60-61	8.47939443556699E-5	9.751303600902038E-4	0.0	3.377777372651155	1.2719091653350483E-4
62-63	8.47939443556699E-5	9.857296031346625E-4	0.0	3.3849848579213866	1.4838940262242232E-4
64-65	8.47939443556699E-5	0.0010387258183569563	0.0	3.3918213696850623	1.4838940262242232E-4
66-67	8.47939443556699E-5	0.0010387258183569563	0.0	3.397979529893893	1.4838940262242232E-4
68-69	8.47939443556699E-5	0.0010599243044458737	0.0	3.40381971281139	1.4838940262242232E-4
70-71	8.47939443556699E-5	0.0010599243044458737	0.0	3.408695364611841	1.4838940262242232E-4
72-73	8.47939443556699E-5	0.0010599243044458737	0.0	3.4127336762117797	1.9078637480025726E-4
74-75	8.47939443556699E-5	0.0011129205196681673	0.0	3.415722662750317	1.9078637480025726E-4
76-77	8.47939443556699E-5	0.0011977144640238373	0.0	3.419135619010633	1.9078637480025726E-4
78-79	8.47939443556699E-5	0.001208313707068296	0.0	3.4213084638347464	1.9078637480025726E-4
80-81	8.47939443556699E-5	0.001208313707068296	0.0	3.422008013875681	1.9078637480025726E-4
82-83	8.47939443556699E-5	0.001208313707068296	0.0	3.422453182083548	1.9078637480025726E-4
84-85	8.47939443556699E-5	0.0012295121931572134	0.0	3.422612170729215	1.9078637480025726E-4
86-87	8.47939443556699E-5	0.001250710679246131	0.0	3.4226439684583485	2.01385617844716E-4
88	8.47939443556699E-5	0.0013143061375128834	0.0	3.4226439684583485	2.1198486088917475E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	4460	0.0	62.557053	94
GGTATCA	5820	0.0	47.98799	1
TATCTCG	5520	0.0	44.5979	94
GTATCAA	10770	0.0	38.033936	1
TCAACGC	12870	0.0	31.522123	4
ATCAACG	12975	0.0	31.167253	3
TATCAAC	13260	0.0	30.891817	2
TGCCGTC	2500	0.0	30.675188	88-89
CAACGCA	13255	0.0	30.606543	5
ATGCCGT	2670	0.0	30.158188	88-89
GCCGTCT	2735	0.0	30.107643	90-91
TATGCCG	2550	0.0	29.954924	86-87
AACGCAG	13695	0.0	29.74966	6
CCGTCTT	2625	0.0	29.673662	90-91
CTCGTAT	2525	0.0	28.967075	82-83
TCTCGTA	2545	0.0	28.739435	82-83
TCGTATG	2815	0.0	28.081833	84-85
CTATCTC	6905	0.0	26.8245	92-93
CGTATGC	2825	0.0	26.654448	84-85
ACGCAGA	15830	0.0	25.682606	7
>>END_MODULE