FastQCFastQC Report
Fri 27 May 2016
SRR2139477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5274208
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78600.049.9442831
GTATCAA146700.037.4459421
TCAACGC172500.031.2020194
TATCAAC175800.030.8850542
ATCAACG176700.030.4847073
CAACGCA177600.030.3060155
AACGCAG182900.029.4742746
ACGCAGA206000.026.189777
CGCAGAG211350.025.5670178
TGGTATC36300.025.0462972
GTGGTAT39200.024.2771841
CTTATAC53700.023.8929081
GCAGAGT240000.022.6034559
TATACAC71950.021.1964443
GAGTACT139450.020.80642112-13
GTACATG172850.020.0811711
CAGAGTA234100.019.67084710-11
TACATGG172700.019.40982
GTACTTT151550.019.07521214-15
ACATGGG173150.018.9160823
Produced by FastQC (version 0.11.5)