##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5274208
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.345365787621574	30.0	18.0	33.0	18.0	34.0
2	30.175004664207403	32.0	28.0	33.0	25.0	33.0
3	31.969388958493862	33.0	31.0	33.0	29.0	34.0
4	32.15096029583968	33.0	32.0	33.0	31.0	34.0
5	32.77491103877587	33.0	33.0	33.0	32.0	34.0
6	36.437414679132864	38.0	37.0	38.0	34.0	38.0
7	37.109198575406964	38.0	38.0	38.0	36.0	38.0
8	36.721281944132656	38.0	37.0	38.0	34.0	38.0
9	37.21457932641261	38.0	38.0	38.0	36.0	38.0
10-11	37.40407820472761	38.0	38.0	38.0	37.0	38.0
12-13	37.406520087944955	38.0	38.0	38.0	37.0	38.0
14-15	37.38062406715852	38.0	38.0	38.0	36.5	38.0
16-17	37.44704323378979	38.0	38.0	38.0	37.0	38.0
18-19	37.4615620961479	38.0	38.0	38.0	37.0	38.0
20-21	37.48723947143331	38.0	38.0	38.0	37.0	38.0
22-23	37.500900914571176	38.0	38.0	38.0	37.0	38.0
24-25	37.517656615915854	38.0	38.0	38.0	37.0	38.0
26-27	37.48897400844483	38.0	38.0	38.0	37.0	38.0
28-29	37.46560015362776	38.0	38.0	38.0	37.0	38.0
30-31	37.470570482317825	38.0	38.0	38.0	37.0	38.0
32-33	37.457561945624505	38.0	38.0	38.0	37.0	38.0
34-35	37.35525752860276	38.0	38.0	38.0	37.0	38.0
36-37	37.3865020690121	38.0	38.0	38.0	37.0	38.0
38-39	37.38113225400925	38.0	38.0	38.0	37.0	38.0
40-41	37.39468539905701	38.0	38.0	38.0	37.0	38.0
42-43	37.36061599491899	38.0	38.0	38.0	37.0	38.0
44-45	37.32317826641106	38.0	38.0	38.0	37.0	38.0
46-47	37.28797453126134	38.0	38.0	38.0	37.0	38.0
48-49	37.21899877266235	38.0	38.0	38.0	37.0	38.0
50-51	37.17654834629707	38.0	38.0	38.0	37.0	38.0
52-53	37.15228376017954	38.0	38.0	38.0	36.5	38.0
54-55	37.11567916850598	38.0	38.0	38.0	36.0	38.0
56-57	37.06200173001648	38.0	38.0	38.0	36.0	38.0
58-59	37.049084435004275	38.0	38.0	38.0	36.0	38.0
60-61	37.04652572663997	38.0	38.0	38.0	36.0	38.0
62-63	37.05234648238474	38.0	38.0	38.0	36.0	38.0
64-65	37.05800180667926	38.0	38.0	38.0	36.0	38.0
66-67	37.05505759602647	38.0	38.0	38.0	36.0	38.0
68-69	37.04361004159942	38.0	38.0	38.0	36.0	38.0
70-71	37.04093290228124	38.0	38.0	38.0	36.0	38.0
72-73	37.03181426075145	38.0	38.0	38.0	36.0	38.0
74-75	37.02765339810277	38.0	38.0	38.0	36.0	38.0
76-77	37.016828394436615	38.0	38.0	38.0	36.0	38.0
78-79	37.000693656599466	38.0	38.0	38.0	36.0	38.0
80-81	36.990167014034455	38.0	38.0	38.0	36.0	38.0
82-83	36.939329931331216	38.0	38.0	38.0	36.0	38.0
84-85	36.93022489864805	38.0	38.0	38.0	35.0	38.0
86-87	36.91694097867685	38.0	38.0	38.0	35.0	38.0
88-89	36.90102806814358	38.0	38.0	38.0	35.0	38.0
90-91	36.8872541982971	38.0	38.0	38.0	35.0	38.0
92-93	36.868952757829526	38.0	38.0	38.0	35.0	38.0
94-95	36.853680532091616	38.0	38.0	38.0	35.0	38.0
96-97	36.83796097946566	38.0	38.0	38.0	35.0	38.0
98-99	36.8606623782333	38.0	38.0	38.0	35.0	38.0
100	35.41164360553094	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	5.0
20	22.0
21	115.0
22	331.0
23	900.0
24	2006.0
25	4098.0
26	7528.0
27	12732.0
28	20423.0
29	30350.0
30	40314.0
31	51934.0
32	66712.0
33	90055.0
34	137222.0
35	260211.0
36	846604.0
37	3702641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.046801339651374	18.556833556810805	13.50248226842779	22.89388283511003
2	14.83054591569144	19.95127628598484	43.22731652855673	21.990861269766988
3	18.66020371016427	26.05929664629059	29.9824047810457	25.298094862499433
4	12.781501980960932	18.129319131896203	38.38195232345785	30.707226563685015
5	13.906770457289511	37.177695684356785	35.28400851843537	13.631525339918335
6	30.33837497497254	37.29153268130494	18.638722629065825	13.731369714656683
7	27.200387242975626	32.46233368118967	22.396026095292413	17.94125298054229
8	24.58791917194013	36.69593614813826	20.731397017334167	17.984747662587445
9	25.659511342745677	17.597902851006257	21.15860807916563	35.583977727082434
10-11	24.748682645811463	26.733283935711295	28.237452902881344	20.280580515595897
12-13	25.6976402902578	24.092859060545205	28.50974023019191	21.69976041900509
14-15	22.723753026046754	25.407691543450696	26.3281140978892	25.54044133261335
16-17	22.143068684435654	28.749596147895573	26.973007132066083	22.134328035602692
18-19	22.162777804743385	27.58562043817764	28.940686449984526	21.31091530709445
20-21	22.966195508970994	26.64288267851998	28.928081402519634	21.46284040998939
22-23	22.68619637037696	26.67594320008358	28.82009472720374	21.817765702335716
24-25	22.54673699133356	26.842049771629405	28.87488560611031	21.736327630926723
26-27	22.440531754012184	26.943952508091506	28.894988520361238	21.720527217535075
28-29	22.394509930848606	26.90549562797542	28.9173820253433	21.78261241583267
30-31	22.55262128908746	26.914134582352077	28.912914631754	21.62032949680646
32-33	22.413669256056988	27.030274165087402	28.81893527469264	21.73712130416296
34-35	22.667938728128057	26.931726320701816	28.8269306388169	21.573404312353222
36-37	22.373807059520296	26.999354788105308	28.799722952505718	21.827115199868675
38-39	22.23862067517162	26.890289115536486	28.97179227394596	21.89929793534593
40-41	22.54237800934146	26.761773748761915	28.843694126221624	21.852154115675003
42-43	22.553416139730768	26.823817769436264	28.643559908448164	21.979206182384804
44-45	22.43046221499035	27.053074550896937	28.57441821818487	21.942045015927846
46-47	22.642702598206846	26.938150267839333	28.172462099326985	22.24668503462684
48-49	22.598040743093723	27.003177728468682	28.134063131166137	22.264718397271462
50-51	22.42930097105232	27.19405213655861	28.164144589970437	22.21250230241863
52-53	22.60614481321748	27.1148467196693	28.19902851691809	22.079979950195128
54-55	22.636031074768727	27.08035838716138	27.980294879484617	22.303315658585273
56-57	22.675289912151094	27.315518016150335	27.797827775938444	22.211364295760127
58-59	22.513037088801102	27.416367178307166	28.082952306146392	21.98764342674534
60-61	22.659519201015847	27.5977809407061	27.80707814852405	21.935621709754
62-63	22.47969557863348	27.814987636777456	27.784502252431064	21.920814532158
64-65	22.488685480080758	27.7603558380699	27.75629317382601	21.994665508023335
66-67	22.695408542635885	27.737388519024847	27.547027764127975	22.02017517421129
68-69	22.528101006296747	27.666385254742526	27.805338302010345	22.000175436950382
70-71	22.69889581958101	27.322809951682313	27.82182199148851	22.156472237248163
72-73	22.874419916264983	27.15208846890269	27.78921630630185	22.184275308530477
74-75	22.602393132647872	27.18712696054212	27.895372237028592	22.315107669781415
76-77	22.63614449167421	27.254055599033034	27.812146350421692	22.29765355887107
78-79	22.697808889506437	27.202864736959516	27.676022596189704	22.423303777344348
80-81	22.62510419711859	27.26264689078818	27.71618383199865	22.396065080094584
82-83	22.658546986869865	27.249526571191858	27.82202082711019	22.269905614828094
84-85	22.779276644203634	27.250767224469502	27.697339952510884	22.272616178815984
86-87	22.605214884068452	27.3355980017913	27.75074597556361	22.308441138576633
88-89	22.70420698112397	27.327548499857528	27.720254116445638	22.247990402572864
90-91	22.718772309425887	27.296294868900866	27.68326992638922	22.30166289528403
92-93	22.765784038957488	27.329012560069916	27.683601209150016	22.221602191822583
94-95	22.626655269642658	27.435269117026827	27.64320640169003	22.294869211640485
96-97	22.737494406686622	27.49059325170794	27.74463998771156	22.02727235389388
98-99	23.812889319581654	29.39207752909685	27.91741458597657	18.877618565344925
100	30.45803972311085	40.36467283876697	29.17728743812218	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	843.0
1	772.5
2	952.0
3	1309.5
4	1741.5
5	2591.5
6	3370.5
7	4346.0
8	5659.5
9	6581.0
10	6658.0
11	6429.5
12	6913.0
13	8572.5
14	12043.5
15	16136.5
16	19635.5
17	22333.5
18	22965.5
19	21584.0
20	19408.0
21	17387.5
22	16735.5
23	18334.0
24	22284.0
25	28641.5
26	37366.5
27	47185.0
28	57107.5
29	68375.0
30	80130.0
31	91358.5
32	103700.5
33	116983.5
34	130071.0
35	143335.0
36	157387.5
37	169028.0
38	177920.0
39	186122.5
40	194488.0
41	201428.5
42	206584.5
43	214964.0
44	224703.5
45	228594.0
46	225750.0
47	222527.5
48	221731.0
49	221033.5
50	217990.5
51	212977.5
52	205645.0
53	195044.5
54	182907.0
55	171097.0
56	157877.0
57	143231.5
58	129060.0
59	111959.0
60	93384.5
61	75662.5
62	59611.0
63	46099.5
64	34949.5
65	26655.0
66	20052.5
67	15245.5
68	11756.5
69	8894.0
70	6558.5
71	4693.0
72	3265.5
73	2155.5
74	1489.0
75	996.5
76	629.0
77	382.0
78	244.0
79	173.5
80	111.5
81	64.0
82	44.5
83	30.5
84	18.0
85	11.5
86	12.0
87	11.5
88	6.5
89	3.5
90	3.5
91	3.0
92	2.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007394475151529859
3	9.100892494190596E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.63615300314364E-5
22-23	0.0
24-25	2.275546709578421E-4
26-27	3.1292490816839266E-4
28-29	0.0022860852630122125
30-31	0.003389691785865289
32-33	0.002663878220426599
34-35	6.486822687471561E-4
36-37	0.0
38-39	2.8900659378177267E-5
40-41	3.200049959567854E-4
42-43	0.002489245240343461
44-45	2.853298353863307E-4
46-47	6.056644118740408E-4
48-49	0.0012723977062376343
50-51	0.0012023456854987008
52-53	0.0
54-55	0.0031378555300550677
56-57	0.05392392118054737
58-59	0.06997845942984227
60-61	0.046462023575089294
62-63	0.007949311796280549
64-65	0.0
66-67	0.0
68-69	1.2001269516109904E-4
70-71	0.0014124552511003358
72-73	0.007869842406684879
74-75	0.01219273353648546
76-77	0.013299380662973556
78-79	0.0037579986760812004
80-81	0.008059854757700905
82-83	0.04642893860817403
84-85	0.03902341416704037
86-87	0.008835976264029507
88-89	0.0020931923094420638
90-91	0.0028506313230795433
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	32.0
20-21	196.0
22-23	433.0
24-25	505.0
26-27	1358.0
28-29	3358.0
30-31	9933.0
32-33	12994.0
34-35	18016.0
36-37	28754.0
38-39	34186.0
40-41	33468.0
42-43	38141.0
44-45	45246.0
46-47	45991.0
48-49	43042.0
50-51	38135.0
52-53	35178.0
54-55	37776.0
56-57	38736.0
58-59	40794.0
60-61	40669.0
62-63	40199.0
64-65	43674.0
66-67	47973.0
68-69	49946.0
70-71	55415.0
72-73	42742.0
74-75	41758.0
76-77	43546.0
78-79	45259.0
80-81	45510.0
82-83	42895.0
84-85	41644.0
86-87	43064.0
88-89	45078.0
90-91	46240.0
92-93	45210.0
94-95	51799.0
96-97	162173.0
98-99	1221138.0
100-101	2572004.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.32895151738288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68306172208864	78.2857363885403
2	6.629570924172061	11.446478137878021
3	1.5067428840103516	3.902266001486739
4	0.5566314522964887	1.9221363863341598
5	0.23232113357561007	1.0028019938456139
6	0.13332720884839966	0.6905998889172885
7	0.07230767703912824	0.43695721618118366
8	0.041478596145674895	0.2864642972535258
9	0.026518051349589577	0.20603480123647694
>10	0.1149542000583318	1.52523880152278
>50	0.002215189101945487	0.1258265051515898
>100	8.021266400778051E-4	0.12417830184555119
>500	6.883467361726223E-5	0.0452812798067869
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.688057808869123E-5	0.0	1.896019269623041E-5	0.0
3	0.0	5.688057808869123E-5	0.0	3.792038539246082E-5	0.0
4	0.0	7.584077078492164E-5	0.0	3.792038539246082E-5	0.0
5	0.0	7.584077078492164E-5	0.0	3.792038539246082E-5	0.0
6	5.688057808869123E-5	7.584077078492164E-5	0.0	3.792038539246082E-5	0.0
7	5.688057808869123E-5	7.584077078492164E-5	0.0	3.792038539246082E-5	0.0
8	5.688057808869123E-5	9.480096348115205E-5	0.0	3.792038539246082E-5	0.0
9	5.688057808869123E-5	1.706417342660737E-4	0.0	5.688057808869123E-5	0.0
10-11	6.636067443680643E-5	1.706417342660737E-4	0.0	6.636067443680643E-5	0.0
12-13	7.584077078492164E-5	1.801218306141889E-4	0.0	8.532086713303685E-5	0.0
14-15	7.584077078492164E-5	3.033630831396866E-4	0.0	1.1376115617738247E-4	0.0
16-17	1.1376115617738247E-4	3.3180337218403216E-4	0.0	1.1376115617738247E-4	0.0
18-19	1.2324125252549767E-4	3.602436612283778E-4	0.0	1.1376115617738247E-4	0.0
20-21	1.6116163791795847E-4	3.981640466208386E-4	0.0	1.1376115617738247E-4	0.0
22-23	1.896019269623041E-4	3.981640466208386E-4	0.0	1.2324125252549767E-4	0.0
24-25	1.896019269623041E-4	3.981640466208386E-4	0.0	1.3272134887361287E-4	0.0
26-27	2.2752231235476493E-4	3.981640466208386E-4	0.0	2.0856211965853452E-4	0.0
28-29	2.4648250505099534E-4	3.981640466208386E-4	0.0	8.152882859379076E-4	0.0
30-31	2.4648250505099534E-4	4.929650101019907E-4	0.0	0.0022467828345033034	0.0
32-33	2.4648250505099534E-4	4.929650101019907E-4	0.0	0.005479495689210589	1.896019269623041E-5
34-35	2.4648250505099534E-4	5.024451064501059E-4	0.0	0.009897220587432275	1.896019269623041E-5
36-37	2.6544269774722573E-4	5.308853954944515E-4	0.0	0.016201484658928886	1.896019269623041E-5
38-39	2.7492279409534095E-4	5.593256845387971E-4	0.0	0.03258309114847196	1.896019269623041E-5
40-41	2.8440289044345617E-4	6.162062626274883E-4	0.0	0.05053839363180216	1.896019269623041E-5
42-43	2.8440289044345617E-4	6.541266480199492E-4	0.0	0.062037750502065905	2.8440289044345613E-5
44-45	3.3180337218403216E-4	6.825669370642948E-4	0.0	0.07566064895430746	3.792038539246082E-5
46-47	3.412834685321474E-4	7.204873224567555E-4	0.0	0.09011779588518315	4.7400481740576026E-5
48-49	3.6024366122837777E-4	7.299674188048708E-4	0.0	0.10411041809500118	5.688057808869123E-5
50-51	3.886839502727234E-4	8.152882859379076E-4	0.0	0.11842536358065514	5.688057808869123E-5
52-53	3.981640466208386E-4	8.342484786341381E-4	0.0	0.15070509164598742	6.636067443680643E-5
54-55	3.981640466208386E-4	8.342484786341381E-4	0.0	0.1904266953445901	7.584077078492164E-5
56-57	3.981640466208386E-4	8.532086713303685E-4	0.0	0.2467195074596982	7.584077078492164E-5
58-59	3.981640466208386E-4	8.532086713303685E-4	0.0	0.28484845497181754	7.584077078492164E-5
60-61	3.981640466208386E-4	8.532086713303685E-4	0.0	0.3013817430029305	7.584077078492164E-5
62-63	4.6452472105764503E-4	8.721688640265988E-4	0.0	0.3147865992391654	7.584077078492164E-5
64-65	4.929650101019907E-4	9.480096348115205E-4	0.0	0.32079698032387044	7.584077078492164E-5
66-67	5.214052991463364E-4	9.764499238558662E-4	0.0	0.32698748323918964	7.584077078492164E-5
68-69	5.308853954944515E-4	9.859300202039814E-4	0.0	0.3330642249983315	7.584077078492164E-5
70-71	5.308853954944515E-4	9.859300202039814E-4	0.0	0.33823087750805425	7.584077078492164E-5
72-73	5.782858772350275E-4	9.954101165520965E-4	0.0	0.34196603546921167	9.480096348115205E-5
74-75	5.877659735831427E-4	0.001023850405596442	0.0	0.3447721439882538	9.480096348115205E-5
76-77	5.877659735831427E-4	0.0010807309836851334	0.0	0.3474550112547704	9.480096348115205E-5
78-79	5.877659735831427E-4	0.0010996911763813638	0.0	0.3495975130294444	9.480096348115205E-5
80-81	5.877659735831427E-4	0.0011091712727294791	0.0	0.3502042391957238	9.480096348115205E-5
82-83	5.877659735831427E-4	0.0011186513690775942	0.0	0.35048864208616726	9.480096348115205E-5
84-85	5.877659735831427E-4	0.0011281314654257094	0.0	0.3505739629533003	1.0428105982926726E-4
86-87	5.877659735831427E-4	0.0011755319471662853	0.0	0.35063084353138896	1.1376115617738247E-4
88	6.067261662793731E-4	0.0012324125252549767	0.0	0.35063084353138896	1.1376115617738247E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7860	0.0	49.944283	1
GTATCAA	14670	0.0	37.445942	1
TCAACGC	17250	0.0	31.202019	4
TATCAAC	17580	0.0	30.885054	2
ATCAACG	17670	0.0	30.484707	3
CAACGCA	17760	0.0	30.306015	5
AACGCAG	18290	0.0	29.474274	6
ACGCAGA	20600	0.0	26.18977	7
CGCAGAG	21135	0.0	25.567017	8
TGGTATC	3630	0.0	25.046297	2
GTGGTAT	3920	0.0	24.277184	1
CTTATAC	5370	0.0	23.892908	1
GCAGAGT	24000	0.0	22.603455	9
TATACAC	7195	0.0	21.196444	3
GAGTACT	13945	0.0	20.806421	12-13
GTACATG	17285	0.0	20.081171	1
CAGAGTA	23410	0.0	19.670847	10-11
TACATGG	17270	0.0	19.4098	2
GTACTTT	15155	0.0	19.075212	14-15
ACATGGG	17315	0.0	18.916082	3
>>END_MODULE